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Protein

Tripeptidyl-peptidase 2

Gene

TPP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Stimulates adipogenesis (By similarity).By similarity

Miscellaneous

The limitation of proteolytic products to tripeptides is achieved by tailoring the size of the substrate-binding cleft: the two negatively charged residues Glu-305 and Glu-331 that are blocking position P4 limit the number of residues that can be accommodated in the binding cleft and thus create a molecular ruler. At the same time, they orient substrates so that the tripeptides are removed exclusively from the N-terminus (By similarity).By similarity

Catalytic activityi

Release of an N-terminal tripeptide from a polypeptide.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Charge relay systemBy similarity1
Active sitei264Charge relay systemBy similarity1
Active sitei449Charge relay systemBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: Reactome
  • endopeptidase activity Source: ProtInc
  • identical protein binding Source: IntAct
  • serine-type endopeptidase activity Source: InterPro
  • tripeptidyl-peptidase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.14.10 2681
ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP29144

Protein family/group databases

MEROPSiS08.A56

Names & Taxonomyi

Protein namesi
Recommended name:
Tripeptidyl-peptidase 2 (EC:3.4.14.10)
Short name:
TPP-2
Alternative name(s):
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase II
Short name:
TPP-II
Gene namesi
Name:TPP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000134900.11
HGNCiHGNC:12016 TPP2
MIMi190470 gene
neXtProtiNX_P29144

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi7174
MalaCardsiTPP2
OpenTargetsiENSG00000134900
PharmGKBiPA36695

Chemistry databases

ChEMBLiCHEMBL6156
GuidetoPHARMACOLOGYi2423

Polymorphism and mutation databases

BioMutaiTPP2
DMDMi34223721

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000764222 – 1249Tripeptidyl-peptidase 2Add BLAST1248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei401N6-acetyllysineBy similarity1
Modified residuei915PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP29144
MaxQBiP29144
PaxDbiP29144
PeptideAtlasiP29144
PRIDEiP29144
ProteomicsDBi54528

PTM databases

iPTMnetiP29144
PhosphoSitePlusiP29144
SwissPalmiP29144

Expressioni

Gene expression databases

BgeeiENSG00000134900 Expressed in 228 organ(s), highest expression level in testis
CleanExiHS_TPP2
ExpressionAtlasiP29144 baseline and differential
GenevisibleiP29144 HS

Organism-specific databases

HPAiHPA021069
HPA049630

Interactioni

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113027, 39 interactors
DIPiDIP-50761N
IntActiP29144, 6 interactors
MINTiP29144
STRINGi9606.ENSP00000365233

Chemistry databases

BindingDBiP29144

Structurei

3D structure databases

ProteinModelPortaliP29144
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 509Peptidase S8Add BLAST470

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Phylogenomic databases

eggNOGiKOG1114 Eukaryota
COG1404 LUCA
GeneTreeiENSGT00390000014623
HOGENOMiHOG000008178
HOVERGENiHBG017992
InParanoidiP29144
KOiK01280
PhylomeDBiP29144
TreeFamiTF105647

Family and domain databases

CDDicd04857 Peptidases_S8_Tripeptidyl_Amin, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR022229 Peptidase_S8A_TPPII
IPR034051 TPP_II_domain
PfamiView protein in Pfam
PF00082 Peptidase_S8, 1 hit
PF12580 TPPII, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 2 hits
PROSITEiView protein in PROSITE
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P29144-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATAATEEPF PFHGLLPKKE TGAASFLCRY PEYDGRGVLI AVLDTGVDPG
60 70 80 90 100
APGMQVTTDG KPKIVDIIDT TGSGDVNTAT EVEPKDGEIV GLSGRVLKIP
110 120 130 140 150
ASWTNPSGKY HIGIKNGYDF YPKALKERIQ KERKEKIWDP VHRVALAEAC
160 170 180 190 200
RKQEEFDVAN NGSSQANKLI KEELQSQVEL LNSFEKKYSD PGPVYDCLVW
210 220 230 240 250
HDGEVWRACI DSNEDGDLSK STVLRNYKEA QEYGSFGTAE MLNYSVNIYD
260 270 280 290 300
DGNLLSIVTS GGAHGTHVAS IAAGHFPEEP ERNGVAPGAQ ILSIKIGDTR
310 320 330 340 350
LSTMETGTGL IRAMIEVINH KCDLVNYSYG EATHWPNSGR ICEVINEAVW
360 370 380 390 400
KHNIIYVSSA GNNGPCLSTV GCPGGTTSSV IGVGAYVSPD MMVAEYSLRE
410 420 430 440 450
KLPANQYTWS SRGPSADGAL GVSISAPGGA IASVPNWTLR GTQLMNGTSM
460 470 480 490 500
SSPNACGGIA LILSGLKANN IDYTVHSVRR ALENTAVKAD NIEVFAQGHG
510 520 530 540 550
IIQVDKAYDY LVQNTSFANK LGFTVTVGNN RGIYLRDPVQ VAAPSDHGVG
560 570 580 590 600
IEPVFPENTE NSEKISLQLH LALTSNSSWV QCPSHLELMN QCRHINIRVD
610 620 630 640 650
PRGLREGLHY TEVCGYDIAS PNAGPLFRVP ITAVIAAKVN ESSHYDLAFT
660 670 680 690 700
DVHFKPGQIR RHFIEVPEGA TWAEVTVCSC SSEVSAKFVL HAVQLVKQRA
710 720 730 740 750
YRSHEFYKFC SLPEKGTLTE AFPVLGGKAI EFCIARWWAS LSDVNIDYTI
760 770 780 790 800
SFHGIVCTAP QLNIHASEGI NRFDVQSSLK YEDLAPCITL KNWVQTLRPV
810 820 830 840 850
SAKTKPLGSR DVLPNNRQLY EMVLTYNFHQ PKSGEVTPSC PLLCELLYES
860 870 880 890 900
EFDSQLWIIF DQNKRQMGSG DAYPHQYSLK LEKGDYTIRL QIRHEQISDL
910 920 930 940 950
ERLKDLPFIV SHRLSNTLSL DIHENHSFAL LGKKKSSNLT LPPKYNQPFF
960 970 980 990 1000
VTSLPDDKIP KGAGPGCYLA GSLTLSKTEL GKKADVIPVH YYLIPPPTKT
1010 1020 1030 1040 1050
KNGSKDKEKD SEKEKDLKEE FTEALRDLKI QWMTKLDSSD IYNELKETYP
1060 1070 1080 1090 1100
NYLPLYVARL HQLDAEKERM KRLNEIVDAA NAVISHIDQT ALAVYIAMKT
1110 1120 1130 1140 1150
DPRPDAATIK NDMDKQKSTL VDALCRKGCA LADHLLHTQA QDGAISTDAE
1160 1170 1180 1190 1200
GKEEEGESPL DSLAETFWET TKWTDLFDNK VLTFAYKHAL VNKMYGRGLK
1210 1220 1230 1240
FATKLVEEKP TKENWKNCIQ LMKLLGWTHC ASFTENWLPI MYPPDYCVF
Length:1,249
Mass (Da):138,350
Last modified:January 23, 2007 - v4
Checksum:iA26A6249DBF7F3DD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5VZU9Q5VZU9_HUMAN
Tripeptidyl-peptidase 2
TPP2
1,262Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252G → R in AAA36760 (PubMed:1670990).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL158063 Genomic DNA No translation available.
CH471085 Genomic DNA Translation: EAX09059.1
BC039905 mRNA Translation: AAH39905.1
M73047 mRNA Translation: AAA36760.1
M55169 mRNA Translation: AAA63263.1
CCDSiCCDS9502.1
PIRiS54376
RefSeqiNP_001317517.1, NM_001330588.1
NP_003282.2, NM_003291.3
UniGeneiHs.432424
Hs.733961

Genome annotation databases

EnsembliENST00000376065; ENSP00000365233; ENSG00000134900
GeneIDi7174
KEGGihsa:7174
UCSCiuc001vpi.5 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL158063 Genomic DNA No translation available.
CH471085 Genomic DNA Translation: EAX09059.1
BC039905 mRNA Translation: AAH39905.1
M73047 mRNA Translation: AAA36760.1
M55169 mRNA Translation: AAA63263.1
CCDSiCCDS9502.1
PIRiS54376
RefSeqiNP_001317517.1, NM_001330588.1
NP_003282.2, NM_003291.3
UniGeneiHs.432424
Hs.733961

3D structure databases

ProteinModelPortaliP29144
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113027, 39 interactors
DIPiDIP-50761N
IntActiP29144, 6 interactors
MINTiP29144
STRINGi9606.ENSP00000365233

Chemistry databases

BindingDBiP29144
ChEMBLiCHEMBL6156
GuidetoPHARMACOLOGYi2423

Protein family/group databases

MEROPSiS08.A56

PTM databases

iPTMnetiP29144
PhosphoSitePlusiP29144
SwissPalmiP29144

Polymorphism and mutation databases

BioMutaiTPP2
DMDMi34223721

Proteomic databases

EPDiP29144
MaxQBiP29144
PaxDbiP29144
PeptideAtlasiP29144
PRIDEiP29144
ProteomicsDBi54528

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376065; ENSP00000365233; ENSG00000134900
GeneIDi7174
KEGGihsa:7174
UCSCiuc001vpi.5 human

Organism-specific databases

CTDi7174
DisGeNETi7174
EuPathDBiHostDB:ENSG00000134900.11
GeneCardsiTPP2
HGNCiHGNC:12016 TPP2
HPAiHPA021069
HPA049630
MalaCardsiTPP2
MIMi190470 gene
neXtProtiNX_P29144
OpenTargetsiENSG00000134900
PharmGKBiPA36695
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1114 Eukaryota
COG1404 LUCA
GeneTreeiENSGT00390000014623
HOGENOMiHOG000008178
HOVERGENiHBG017992
InParanoidiP29144
KOiK01280
PhylomeDBiP29144
TreeFamiTF105647

Enzyme and pathway databases

BRENDAi3.4.14.10 2681
ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP29144

Miscellaneous databases

ChiTaRSiTPP2 human
GeneWikiiTripeptidyl_peptidase_II
GenomeRNAii7174
PROiPR:P29144
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134900 Expressed in 228 organ(s), highest expression level in testis
CleanExiHS_TPP2
ExpressionAtlasiP29144 baseline and differential
GenevisibleiP29144 HS

Family and domain databases

CDDicd04857 Peptidases_S8_Tripeptidyl_Amin, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR022229 Peptidase_S8A_TPPII
IPR034051 TPP_II_domain
PfamiView protein in Pfam
PF00082 Peptidase_S8, 1 hit
PF12580 TPPII, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 2 hits
PROSITEiView protein in PROSITE
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPP2_HUMAN
AccessioniPrimary (citable) accession number: P29144
Secondary accession number(s): Q5VZU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 171 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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