UniProtKB - P29131 (FTSN_ECOLI)
Protein
Cell division protein FtsN
Gene
ftsN
Organism
Escherichia coli (strain K12)
Status
Functioni
Essential cell division protein that activates septal peptidoglycan synthesis and constriction of the cell. Acts on both sides of the membrane, via interaction with FtsA in the cytoplasm and interaction with the FtsQBL complex in the periplasm. These interactions may induce a conformational switch in both FtsA and FtsQBL, leading to septal peptidoglycan synthesis by FtsI and associated synthases (Probable) (PubMed:25496160). Required for full FtsI activity (PubMed:25496160). Required for recruitment of AmiC to the septal ring (PubMed:12787347).2 Publications2 Publications
GO - Molecular functioni
- peptidoglycan binding Source: InterPro
GO - Biological processi
- cell division Source: CACAO
- division septum assembly Source: EcoCyc
- FtsZ-dependent cytokinesis Source: UniProtKB-UniRule
Keywordsi
Biological process | Cell cycle, Cell division, Septation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11529-MONOMER MetaCyc:EG11529-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division protein FtsNUniRule annotationCurated |
Gene namesi | Name:ftsNUniRule annotation Synonyms:msgA Ordered Locus Names:b3933, JW3904 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication Note: Localizes to the septum (PubMed:9282742, PubMed:19684127, PubMed:24750258). Localizes to the midcell via interaction with the early cell division protein FtsA and via the periplasmic SPOR domain (PubMed:9282742, PubMed:19684127, PubMed:24750258). FtsA-dependent localization precedes SPOR-dependent localization, and both are needed for efficient localization (PubMed:24750258). Localization depends upon FtsZ, FtsA, ZipA, FtsQ and FtsI (PubMed:9282742, PubMed:11948172).4 Publications
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | CytoplasmicUniRule annotation1 PublicationAdd BLAST | 33 | |
Transmembranei | 34 – 54 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 55 – 319 | PeriplasmicUniRule annotation1 PublicationAdd BLAST | 265 |
GO - Cellular componenti
- cell septum Source: CACAO
- division septum Source: EcoCyc
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Depletion does not affect localization of FtsZ, FtsA, ZipA, FtsQ, FtsL and FtsI to the division site (PubMed:11703663). Cells containing low levels of FtsN stop dividing while their mean cell length increases (PubMed:20345660). Absence of FtsN is followed by an inverse sequential disassembly of already assembled divisome compounds (PubMed:20345660).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 5 | D → N: Causes significant impairment of the interaction with FtsA. 1 Publication | 1 | |
Mutagenesisi | 83 | W → L or T: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 85 | Y → S or W: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 89 | L → S: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 251 | Q → A: Reduces septal localization by a factor of at least 3. 1 Publication | 1 | |
Mutagenesisi | 251 | Q → E: Severe localization defects. Binds peptidoglycan poorly. 1 Publication | 1 | |
Mutagenesisi | 252 | C → A: Severely reduces stability of the protein. 1 Publication | 1 | |
Mutagenesisi | 254 | S → A: Reduces septal localization by a factor of at least 3. 1 Publication | 1 | |
Mutagenesisi | 254 | S → E: Binds peptidoglycan poorly. 1 Publication | 1 | |
Mutagenesisi | 263 | T → D: Intermediate localization defects. 1 Publication | 1 | |
Mutagenesisi | 270 | F → A: Intermediate localization defects. 1 Publication | 1 | |
Mutagenesisi | 283 | W → A: Reduces septal localization by a factor of at least 3. 1 Publication | 1 | |
Mutagenesisi | 283 | W → D: Severe localization defects. 1 Publication | 1 | |
Mutagenesisi | 285 | R → A: Reduces septal localization by a factor of at least 3. Binds peptidoglycan poorly. 1 Publication | 1 | |
Mutagenesisi | 312 | C → A: Severely reduces stability of the protein. 1 Publication | 1 | |
Mutagenesisi | 313 | I → A: Reduces septal localization by a factor of at least 3. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087376 | 1 – 319 | Cell division protein FtsNAdd BLAST | 319 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 252 ↔ 312 | UniRule annotation1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P29131 |
PaxDbi | P29131 |
PRIDEi | P29131 |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP29131
Protein-protein interaction databases
BioGRIDi | 4261592, 198 interactors 852725, 3 interactors |
ComplexPortali | CPX-1936, Divisome complex |
DIPi | DIP-9705N |
IntActi | P29131, 24 interactors |
MINTi | P29131 |
STRINGi | 511145.b3933 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P29131 |
SMRi | P29131 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P29131 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 115 – 120 | 1-1 | 6 | |
Repeati | 145 – 150 | 1-2 | 6 | |
Repeati | 197 – 200 | 2-1 | 4 | |
Repeati | 220 – 223 | 2-2 | 4 | |
Domaini | 242 – 316 | SPORUniRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 4 – 6 | Mediates interaction with FtsAUniRule annotation1 Publication | 3 | |
Regioni | 115 – 150 | 2 X 6 AA repeatsAdd BLAST | 36 | |
Regioni | 197 – 223 | 2 X 4 AA repeatsAdd BLAST | 27 |
Domaini
The cytoplasmic region is required for interaction with FtsA (PubMed:24750258). The periplasmic region is composed of a membrane-proximal region containing three short partially formed helices (H1, H2 and H3), followed by an unstructured glutamine-rich linker, and a C-terminal globular SPOR domain (PubMed:15101973, PubMed:19684127). Essential function of FtsN is accomplished by a small region of at most 35 residues that is centered about the H2 helix (PubMed:19684127). The SPOR domain, which exhibits a ribonucleoprotein (RNP) fold, binds peptidoglycan and is a strong septal localization determinant, but it seems not essential for cell division (PubMed:15466024, PubMed:19684127, PubMed:24056104).5 Publications
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3087, Bacteria |
HOGENOMi | CLU_058902_0_0_6 |
InParanoidi | P29131 |
Family and domain databases
DisProti | DP02271 |
Gene3Di | 3.30.70.1070, 1 hit |
HAMAPi | MF_02039, FtsN_entero, 1 hit |
InterProi | View protein in InterPro IPR011930, FtsN IPR007730, SPOR-like_dom IPR036680, SPOR-like_sf |
Pfami | View protein in Pfam PF05036, SPOR, 1 hit |
SUPFAMi | SSF110997, SSF110997, 1 hit |
TIGRFAMsi | TIGR02223, ftsN, 1 hit |
PROSITEi | View protein in PROSITE PS51724, SPOR, 1 hit |
i Sequence
Sequence statusi: Complete.
P29131-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQRDYVRRS QPAPSRRKKS TSRKKQRNLP AVSPAMVAIA AAVLVTFIGG
60 70 80 90 100
LYFITHHKKE ESETLQSQKV TGNGLPPKPE ERWRYIKELE SRQPGVRAPT
110 120 130 140 150
EPSAGGEVKT PEQLTPEQRQ LLEQMQADMR QQPTQLVEVP WNEQTPEQRQ
160 170 180 190 200
QTLQRQRQAQ QLAEQQRLAQ QSRTTEQSWQ QQTRTSQAAP VQAQPRQSKP
210 220 230 240 250
ASSQQPYQDL LQTPAHTTAQ SKPQQAAPVA RAADAPKPTA EKKDERRWMV
260 270 280 290 300
QCGSFRGAEQ AETVRAQLAF EGFDSKITTN NGWNRVVIGP VKGKENADST
310
LNRLKMAGHT NCIRLAAGG
Sequence cautioni
The sequence AAA23935 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 29 | L → V (PubMed:8509333).Curated | 1 | |
Sequence conflicti | 29 | L → V (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L14281 Genomic DNA Translation: AAA23814.1 L06547 Genomic DNA Translation: AAA23935.1 Frameshift. L19201 Genomic DNA Translation: AAB03065.1 U00096 Genomic DNA Translation: AAC76915.1 AP009048 Genomic DNA Translation: BAE77377.1 |
PIRi | S40876 |
RefSeqi | NP_418368.1, NC_000913.3 WP_000068828.1, NZ_SSZK01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC76915; AAC76915; b3933 BAE77377; BAE77377; BAE77377 |
GeneIDi | 57732226 948428 |
KEGGi | ecj:JW3904 eco:b3933 |
PATRICi | fig|511145.12.peg.4051 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L14281 Genomic DNA Translation: AAA23814.1 L06547 Genomic DNA Translation: AAA23935.1 Frameshift. L19201 Genomic DNA Translation: AAB03065.1 U00096 Genomic DNA Translation: AAC76915.1 AP009048 Genomic DNA Translation: BAE77377.1 |
PIRi | S40876 |
RefSeqi | NP_418368.1, NC_000913.3 WP_000068828.1, NZ_SSZK01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UTA | NMR | - | A | 243-319 | [»] | |
6YN0 | X-ray | 2.40 | B | 75-93 | [»] | |
BMRBi | P29131 | |||||
SMRi | P29131 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261592, 198 interactors 852725, 3 interactors |
ComplexPortali | CPX-1936, Divisome complex |
DIPi | DIP-9705N |
IntActi | P29131, 24 interactors |
MINTi | P29131 |
STRINGi | 511145.b3933 |
Proteomic databases
jPOSTi | P29131 |
PaxDbi | P29131 |
PRIDEi | P29131 |
Genome annotation databases
EnsemblBacteriai | AAC76915; AAC76915; b3933 BAE77377; BAE77377; BAE77377 |
GeneIDi | 57732226 948428 |
KEGGi | ecj:JW3904 eco:b3933 |
PATRICi | fig|511145.12.peg.4051 |
Organism-specific databases
EchoBASEi | EB1491 |
Phylogenomic databases
eggNOGi | COG3087, Bacteria |
HOGENOMi | CLU_058902_0_0_6 |
InParanoidi | P29131 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11529-MONOMER MetaCyc:EG11529-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P29131 |
PROi | PR:P29131 |
Family and domain databases
DisProti | DP02271 |
Gene3Di | 3.30.70.1070, 1 hit |
HAMAPi | MF_02039, FtsN_entero, 1 hit |
InterProi | View protein in InterPro IPR011930, FtsN IPR007730, SPOR-like_dom IPR036680, SPOR-like_sf |
Pfami | View protein in Pfam PF05036, SPOR, 1 hit |
SUPFAMi | SSF110997, SSF110997, 1 hit |
TIGRFAMsi | TIGR02223, ftsN, 1 hit |
PROSITEi | View protein in PROSITE PS51724, SPOR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FTSN_ECOLI | |
Accessioni | P29131Primary (citable) accession number: P29131 Secondary accession number(s): Q2M8M9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | August 29, 2003 | |
Last modified: | February 10, 2021 | |
This is version 175 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families