Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

General transcription factor IIE subunit 1

Gene

GTF2E1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri129 – 157C4-typeSequence analysisAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIE subunit 1
Alternative name(s):
General transcription factor IIE 56 kDa subunit
Transcription initiation factor IIE subunit alpha
Short name:
TFIIE-alpha
Gene namesi
Name:GTF2E1
Synonyms:TF2E1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000153767.9
HGNCiHGNC:4650 GTF2E1
MIMi189962 gene
neXtProtiNX_P29083

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2960
OpenTargetsiENSG00000153767
PharmGKBiPA29036

Polymorphism and mutation databases

BioMutaiGTF2E1
DMDMi116242812

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002112222 – 439General transcription factor IIE subunit 1Add BLAST438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei67N6-acetyllysineCombined sources1
Modified residuei268PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP29083
MaxQBiP29083
PaxDbiP29083
PeptideAtlasiP29083
PRIDEiP29083
ProteomicsDBi54517

PTM databases

iPTMnetiP29083
PhosphoSitePlusiP29083

Miscellaneous databases

PMAP-CutDBiP29083

Expressioni

Gene expression databases

BgeeiENSG00000153767 Expressed in 216 organ(s), highest expression level in oocyte
CleanExiHS_GTF2E1
ExpressionAtlasiP29083 baseline and differential
GenevisibleiP29083 HS

Organism-specific databases

HPAiCAB009884
HPA056359
HPA066881

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains (PubMed:1956398). Interacts with TAF6/TAFII80 (PubMed:7667268). Interacts with ATF7IP (PubMed:19106100). Interacts with SND1 (PubMed:7651391).4 Publications
(Microbial infection) Interacts with varicella-zoster virus IE63 protein.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi109215, 52 interactors
CORUMiP29083
DIPiDIP-717N
IntActiP29083, 12 interactors
MINTiP29083
STRINGi9606.ENSP00000283875

Structurei

Secondary structure

1439
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP29083
SMRiP29083
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29083

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 104HTH TFE/IIEalpha-typePROSITE-ProRule annotationAdd BLAST91

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi378 – 393Asp/Glu-rich (acidic)Add BLAST16

Sequence similaritiesi

Belongs to the TFIIE alpha subunit family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri129 – 157C4-typeSequence analysisAdd BLAST29

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2593 Eukaryota
COG1675 LUCA
GeneTreeiENSGT00390000016696
HOGENOMiHOG000007666
HOVERGENiHBG008614
InParanoidiP29083
KOiK03136
OMAiEFRCTYC
OrthoDBiEOG091G0D8N
PhylomeDBiP29083
TreeFamiTF313429

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR017919 TFIIE/TFIIEa_HTH
IPR002853 TFIIE_asu
IPR021600 TFIIE_asu_C
IPR024550 TFIIEa/SarR/Rpc3_HTH_dom
IPR013083 Znf_RING/FYVE/PHD
IPR013137 Znf_TFIIB
PfamiView protein in Pfam
PF08271 TF_Zn_Ribbon, 1 hit
PF11521 TFIIE-A_C, 1 hit
PF02002 TFIIE_alpha, 1 hit
SMARTiView protein in SMART
SM00531 TFIIE, 1 hit
PROSITEiView protein in PROSITE
PS51344 HTH_TFE_IIE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P29083-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE
60 70 80 90 100
LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV
110 120 130 140 150
NVVKYKLDHM RRRIETDERD STNRASFKCP VCSSTFTDLE ANQLFDPMTG
160 170 180 190 200
TFRCTFCHTE VEEDESAMPK KDARTLLARF NEQIEPIYAL LRETEDVNLA
210 220 230 240 250
YEILEPEPTE IPALKQSKDH AATTAGAASL AGGHHREAWA TKGPSYEDLY
260 270 280 290 300
TQNVVINMDD QEDLHRASLE GKSAKERPIW LRESTVQGAY GSEDMKEGGI
310 320 330 340 350
DMDAFQEREE GHAGPDDNEE VMRALLIHEK KTSSAMAGSV GAAAPVTAAN
360 370 380 390 400
GSDSESETSE SDDDSPPRPA AVAVHKREED EEEDDEFEEV ADDPIVMVAG
410 420 430
RPFSYSEVSQ RPELVAQMTP EEKEAYIAMG QRMFEDLFE
Length:439
Mass (Da):49,452
Last modified:October 17, 2006 - v2
Checksum:i9234FFE3F150340B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PER7E9PER7_HUMAN
General transcription factor IIE su...
GTF2E1
122Annotation score:
C9J329C9J329_HUMAN
General transcription factor IIE su...
GTF2E1
49Annotation score:
C9IYL4C9IYL4_HUMAN
General transcription factor IIE su...
GTF2E1
129Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti352S → D in CAA45068 (PubMed:1956403).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020321366P → S. Corresponds to variant dbSNP:rs3732401Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67859 mRNA Translation: AAB20413.1
X63468 mRNA Translation: CAA45068.1
CCDSiCCDS3002.1
PIRiS29291
RefSeqiNP_005504.2, NM_005513.2
XP_011511046.1, XM_011512744.2
XP_011511047.1, XM_011512745.2
UniGeneiHs.445272

Genome annotation databases

EnsembliENST00000283875; ENSP00000283875; ENSG00000153767
GeneIDi2960
KEGGihsa:2960
UCSCiuc003edz.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67859 mRNA Translation: AAB20413.1
X63468 mRNA Translation: CAA45068.1
CCDSiCCDS3002.1
PIRiS29291
RefSeqiNP_005504.2, NM_005513.2
XP_011511046.1, XM_011512744.2
XP_011511047.1, XM_011512745.2
UniGeneiHs.445272

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VD4NMR-A113-174[»]
2JTXNMR-A336-439[»]
2RNQNMR-A378-439[»]
2RNRNMR-A378-439[»]
5GPYX-ray2.10A1-217[»]
5IY6electron microscopy7.20Q1-439[»]
5IY7electron microscopy8.60Q1-439[»]
5IY8electron microscopy7.90Q1-439[»]
5IY9electron microscopy6.30Q1-439[»]
5IYAelectron microscopy5.40Q1-439[»]
5IYBelectron microscopy3.90Q1-439[»]
5IYCelectron microscopy3.90Q1-439[»]
5IYDelectron microscopy3.90Q1-439[»]
ProteinModelPortaliP29083
SMRiP29083
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109215, 52 interactors
CORUMiP29083
DIPiDIP-717N
IntActiP29083, 12 interactors
MINTiP29083
STRINGi9606.ENSP00000283875

PTM databases

iPTMnetiP29083
PhosphoSitePlusiP29083

Polymorphism and mutation databases

BioMutaiGTF2E1
DMDMi116242812

Proteomic databases

EPDiP29083
MaxQBiP29083
PaxDbiP29083
PeptideAtlasiP29083
PRIDEiP29083
ProteomicsDBi54517

Protocols and materials databases

DNASUi2960
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000283875; ENSP00000283875; ENSG00000153767
GeneIDi2960
KEGGihsa:2960
UCSCiuc003edz.5 human

Organism-specific databases

CTDi2960
DisGeNETi2960
EuPathDBiHostDB:ENSG00000153767.9
GeneCardsiGTF2E1
HGNCiHGNC:4650 GTF2E1
HPAiCAB009884
HPA056359
HPA066881
MIMi189962 gene
neXtProtiNX_P29083
OpenTargetsiENSG00000153767
PharmGKBiPA29036
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2593 Eukaryota
COG1675 LUCA
GeneTreeiENSGT00390000016696
HOGENOMiHOG000007666
HOVERGENiHBG008614
InParanoidiP29083
KOiK03136
OMAiEFRCTYC
OrthoDBiEOG091G0D8N
PhylomeDBiP29083
TreeFamiTF313429

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Miscellaneous databases

ChiTaRSiGTF2E1 human
EvolutionaryTraceiP29083
GeneWikiiGTF2E1
GenomeRNAii2960
PMAP-CutDBiP29083
PROiPR:P29083
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000153767 Expressed in 216 organ(s), highest expression level in oocyte
CleanExiHS_GTF2E1
ExpressionAtlasiP29083 baseline and differential
GenevisibleiP29083 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR017919 TFIIE/TFIIEa_HTH
IPR002853 TFIIE_asu
IPR021600 TFIIE_asu_C
IPR024550 TFIIEa/SarR/Rpc3_HTH_dom
IPR013083 Znf_RING/FYVE/PHD
IPR013137 Znf_TFIIB
PfamiView protein in Pfam
PF08271 TF_Zn_Ribbon, 1 hit
PF11521 TFIIE-A_C, 1 hit
PF02002 TFIIE_alpha, 1 hit
SMARTiView protein in SMART
SM00531 TFIIE, 1 hit
PROSITEiView protein in PROSITE
PS51344 HTH_TFE_IIE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiT2EA_HUMAN
AccessioniPrimary (citable) accession number: P29083
Secondary accession number(s): Q16103
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 17, 2006
Last modified: September 12, 2018
This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again