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UniProtKB - P29059 (CHI3_TOBAC)
Protein
Endochitinase 3
Gene
CHN14
Organism
Nicotiana tabacum (Common tobacco)
Status
Functioni
Defense against chitin-containing fungal pathogens.
Catalytic activityi
- Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. EC:3.2.1.14
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 150 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- chitinase activity Source: GO_Central
- chitin binding Source: UniProtKB-KW
GO - Biological processi
- cell wall macromolecule catabolic process Source: InterPro
- chitin catabolic process Source: UniProtKB-KW
- defense response to fungus Source: GO_Central
- polysaccharide catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation |
Ligand | Chitin-binding |
Protein family/group databases
CAZyi | CBM18, Carbohydrate-Binding Module Family 18 GH19, Glycoside Hydrolase Family 19 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CHN14 |
Organismi | Nicotiana tabacum (Common tobacco) |
Taxonomic identifieri | 4097 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana |
Proteomesi |
|
Subcellular locationi
Vacuole
- Vacuole Curated
Note: Vacuolar and protoplast.Curated
Vacuole
- vacuole Source: UniProtKB-SubCell
Keywords - Cellular componenti
VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Add BLAST | 23 | |
ChainiPRO_0000005334 | 24 – 327 | Endochitinase 3Add BLAST | 304 | |
PropeptideiPRO_0000005335 | 328 – 334 | Removed in mature formCurated | 7 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 26 ↔ 41 | PROSITE-ProRule annotation | ||
Disulfide bondi | 35 ↔ 47 | PROSITE-ProRule annotation | ||
Disulfide bondi | 40 ↔ 54 | PROSITE-ProRule annotation | ||
Disulfide bondi | 59 ↔ 63 | PROSITE-ProRule annotation | ||
Modified residuei | 73 | 4-hydroxyprolineBy similarity | 1 | |
Modified residuei | 74 | 4-hydroxyprolineBy similarity | 1 | |
Modified residuei | 76 | 4-hydroxyprolineBy similarity | 1 | |
Disulfide bondi | 106 ↔ 168 | PROSITE-ProRule annotation | ||
Disulfide bondi | 180 ↔ 188 | PROSITE-ProRule annotation | ||
Disulfide bondi | 287 ↔ 319 | PROSITE-ProRule annotation |
Post-translational modificationi
The 4-hydroxyproline residues are not glycosylated in this plant vacuolar protein.By similarity
Keywords - PTMi
Disulfide bond, HydroxylationProteomic databases
PRIDEi | P29059 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 65 | Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST | 42 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 84 | DisorderedSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 66 – 80 | Pro residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
OMAi | GWASAPN |
OrthoDBi | 1132954at2759 |
PhylomeDBi | P29059 |
Family and domain databases
Gene3Di | 3.30.60.10, 1 hit |
InterProi | View protein in InterPro IPR001002, Chitin-bd_1 IPR018371, Chitin-binding_1_CS IPR036861, Endochitinase-like_sf IPR016283, Glyco_hydro_19 IPR000726, Glyco_hydro_19_cat IPR023346, Lysozyme-like_dom_sf |
Pfami | View protein in Pfam PF00187, Chitin_bind_1, 1 hit PF00182, Glyco_hydro_19, 1 hit |
PIRSFi | PIRSF001060, Endochitinase, 1 hit |
PRINTSi | PR00451, CHITINBINDNG |
SMARTi | View protein in SMART SM00270, ChtBD1, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit SSF57016, SSF57016, 1 hit |
PROSITEi | View protein in PROSITE PS00026, CHIT_BIND_I_1, 1 hit PS50941, CHIT_BIND_I_2, 1 hit PS00773, CHITINASE_19_1, 1 hit PS00774, CHITINASE_19_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P29059-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLLEFTALS SLLVLFLLLA VSAEQCGKQA GGARCPSGMC CSNFGWCGNT
60 70 80 90 100
QDYCGPGKCQ SQCPSGPGPT PRPPTPTPGP STGDISNIIS SSMFDQMLKH
110 120 130 140 150
RNDNTCQGKS FYTYNAFITA ARSFRGFGTT GDTTRRKREV AAFFAQTSHE
160 170 180 190 200
TTGGWDTAPD GRYAWGYCYL REQGNPPSYC VQSSQWPCAP GQKYYGRGPI
210 220 230 240 250
QISYNYNYGP CGRAIGQNLL NNPDLVATNA VVSFKSAIWF WMTAQSPKPS
260 270 280 290 300
CHDVITGRWT PSAADRAANR LPGYGVITNI INGGLECGHG SDARVQDRIG
310 320 330
FYRRYCSILG VSPGDNIDCG NQKSFNSGLL LETM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64518 Genomic DNA Translation: CAA45821.1 |
PIRi | S20982 |
RefSeqi | XP_016461053.1, XM_016605567.1 |
Genome annotation databases
GeneIDi | 107784434 |
KEGGi | nta:107784434 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64518 Genomic DNA Translation: CAA45821.1 |
PIRi | S20982 |
RefSeqi | XP_016461053.1, XM_016605567.1 |
3D structure databases
SMRi | P29059 |
ModBasei | Search... |
Protein family/group databases
CAZyi | CBM18, Carbohydrate-Binding Module Family 18 GH19, Glycoside Hydrolase Family 19 |
Proteomic databases
PRIDEi | P29059 |
Genome annotation databases
GeneIDi | 107784434 |
KEGGi | nta:107784434 |
Phylogenomic databases
OMAi | GWASAPN |
OrthoDBi | 1132954at2759 |
PhylomeDBi | P29059 |
Family and domain databases
Gene3Di | 3.30.60.10, 1 hit |
InterProi | View protein in InterPro IPR001002, Chitin-bd_1 IPR018371, Chitin-binding_1_CS IPR036861, Endochitinase-like_sf IPR016283, Glyco_hydro_19 IPR000726, Glyco_hydro_19_cat IPR023346, Lysozyme-like_dom_sf |
Pfami | View protein in Pfam PF00187, Chitin_bind_1, 1 hit PF00182, Glyco_hydro_19, 1 hit |
PIRSFi | PIRSF001060, Endochitinase, 1 hit |
PRINTSi | PR00451, CHITINBINDNG |
SMARTi | View protein in SMART SM00270, ChtBD1, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit SSF57016, SSF57016, 1 hit |
PROSITEi | View protein in PROSITE PS00026, CHIT_BIND_I_1, 1 hit PS50941, CHIT_BIND_I_2, 1 hit PS00773, CHITINASE_19_1, 1 hit PS00774, CHITINASE_19_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHI3_TOBAC | |
Accessioni | P29059Primary (citable) accession number: P29059 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | September 29, 2021 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families