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Protein

Transcription initiation factor IIB

Gene

SUA7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Miscellaneous

Present with 6750 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24ZincBy similarity1
Metal bindingi45ZincBy similarity1
Metal bindingi48ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri20 – 53TFIIB-typePROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34230-MONOMER
ReactomeiR-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Protein family/group databases

MoonDBiP29055 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIB
Alternative name(s):
General transcription factor TFIIB
Transcription factor E
Gene namesi
Name:SUA7
Ordered Locus Names:YPR086W
ORF Names:P9513.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

SGDiS000006290 SUA7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001193081 – 345Transcription initiation factor IIBAdd BLAST345

Proteomic databases

MaxQBiP29055
PaxDbiP29055
PRIDEiP29055

Interactioni

Subunit structurei

Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.

Binary interactionsi

WithEntry#Exp.IntActNotes
RPB5P204344EBI-19123,EBI-15781

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36257, 403 interactors
DIPiDIP-701N
IntActiP29055, 61 interactors
MINTiP29055
STRINGi4932.YPR086W

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP29055
SMRiP29055
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29055

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati136 – 2121Add BLAST77
Repeati242 – 3182Add BLAST77

Sequence similaritiesi

Belongs to the TFIIB family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri20 – 53TFIIB-typePROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000006671
HOGENOMiHOG000108634
InParanoidiP29055
KOiK03124
OMAiNLGLPNM
OrthoDBiEOG092C36CN

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR000812 TFIIB
IPR023486 TFIIB_CS
IPR013150 TFIIB_cyclin
IPR013137 Znf_TFIIB
PANTHERiPTHR11618 PTHR11618, 1 hit
PfamiView protein in Pfam
PF08271 TF_Zn_Ribbon, 1 hit
PF00382 TFIIB, 2 hits
PRINTSiPR00685 TIFACTORIIB
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00782 TFIIB, 1 hit
PS51134 ZF_TFIIB, 1 hit

Sequencei

Sequence statusi: Complete.

P29055-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL
60 70 80 90 100
VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE
110 120 130 140 150
TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA
160 170 180 190 200
KEAYKLCHDE KTLKGKSMES IMAASILIGC RRAEVARTFK EIQSLIHVKT
210 220 230 240 250
KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP RFCSHLGLPM
260 270 280 290 300
QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ
310 320 330 340
TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK
Length:345
Mass (Da):38,200
Last modified:December 1, 1992 - v1
Checksum:i8F1F6D24602436E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10R → L in AAT93251 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81380 Genomic DNA Translation: AAA35126.1
U51033 Genomic DNA Translation: AAB68135.1
AY693232 Genomic DNA Translation: AAT93251.1
BK006949 Genomic DNA Translation: DAA11504.1
PIRiS26707
RefSeqiNP_015411.1, NM_001184183.1

Genome annotation databases

EnsemblFungiiYPR086W; YPR086W; YPR086W
GeneIDi856201
KEGGisce:YPR086W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81380 Genomic DNA Translation: AAA35126.1
U51033 Genomic DNA Translation: AAB68135.1
AY693232 Genomic DNA Translation: AAT93251.1
BK006949 Genomic DNA Translation: DAA11504.1
PIRiS26707
RefSeqiNP_015411.1, NM_001184183.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K1FX-ray4.30M1-68[»]
3K7AX-ray3.80M1-345[»]
4BBRX-ray3.40M1-345[»]
4BBSX-ray3.60M1-345[»]
4V1Nelectron microscopy7.80M1-345[»]
4V1Oelectron microscopy9.70M1-345[»]
5FMFelectron microscopy6.00P1-345[»]
5FYWelectron microscopy4.35M1-345[»]
5FZ5electron microscopy8.80M1-345[»]
5OQJelectron microscopy4.70M1-345[»]
5OQMelectron microscopy5.80M1-345[»]
5SVAelectron microscopy15.30c1-345[»]
ProteinModelPortaliP29055
SMRiP29055
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36257, 403 interactors
DIPiDIP-701N
IntActiP29055, 61 interactors
MINTiP29055
STRINGi4932.YPR086W

Protein family/group databases

MoonDBiP29055 Predicted

Proteomic databases

MaxQBiP29055
PaxDbiP29055
PRIDEiP29055

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR086W; YPR086W; YPR086W
GeneIDi856201
KEGGisce:YPR086W

Organism-specific databases

SGDiS000006290 SUA7

Phylogenomic databases

GeneTreeiENSGT00390000006671
HOGENOMiHOG000108634
InParanoidiP29055
KOiK03124
OMAiNLGLPNM
OrthoDBiEOG092C36CN

Enzyme and pathway databases

BioCyciYEAST:G3O-34230-MONOMER
ReactomeiR-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Miscellaneous databases

EvolutionaryTraceiP29055
PROiPR:P29055

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR000812 TFIIB
IPR023486 TFIIB_CS
IPR013150 TFIIB_cyclin
IPR013137 Znf_TFIIB
PANTHERiPTHR11618 PTHR11618, 1 hit
PfamiView protein in Pfam
PF08271 TF_Zn_Ribbon, 1 hit
PF00382 TFIIB, 2 hits
PRINTSiPR00685 TIFACTORIIB
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00782 TFIIB, 1 hit
PS51134 ZF_TFIIB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTF2B_YEAST
AccessioniPrimary (citable) accession number: P29055
Secondary accession number(s): D6W488, E9P939
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 7, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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