UniProtKB - P29012 (ALR2_ECOLI)
Protein
Alanine racemase, catabolic
Gene
dadX
Organism
Escherichia coli (strain K12)
Status
Functioni
Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA.By similarity
Catalytic activityi
- EC:5.1.1.1
Cofactori
pyridoxal 5'-phosphateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 35 | Proton acceptor; specific for D-alanineBy similarity | 1 | |
Binding sitei | 130 | SubstrateBy similarity | 1 | |
Active sitei | 253 | Proton acceptor; specific for L-alanineBy similarity | 1 | |
Binding sitei | 301 | Substrate; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- alanine racemase activity Source: EcoCyc
- pyridoxal phosphate binding Source: GO_Central
GO - Biological processi
- D-alanine biosynthetic process Source: GO_Central
- L-alanine oxidation to pyruvate via D-alanine Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:ALARACECAT-MONOMER MetaCyc:ALARACECAT-MONOMER |
SABIO-RKi | P29012 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dadX Synonyms:alnB, dadB Ordered Locus Names:b1190, JW1179 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000114517 | 1 – 356 | Alanine racemase, catabolicAdd BLAST | 356 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 35 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
jPOSTi | P29012 |
PaxDbi | P29012 |
PRIDEi | P29012 |
Expressioni
Inductioni
By alanine.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260105, 648 interactors |
DIPi | DIP-9395N |
IntActi | P29012, 2 interactors |
STRINGi | 511145.b1190 |
Family & Domainsi
Sequence similaritiesi
Belongs to the alanine racemase family.Curated
Phylogenomic databases
eggNOGi | COG0787, Bacteria |
HOGENOMi | CLU_028393_1_0_6 |
InParanoidi | P29012 |
PhylomeDBi | P29012 |
Family and domain databases
Gene3Di | 2.40.37.10, 1 hit 3.20.20.10, 1 hit |
HAMAPi | MF_01201, Ala_racemase, 1 hit |
InterProi | View protein in InterPro IPR000821, Ala_racemase IPR009006, Ala_racemase/Decarboxylase_C IPR011079, Ala_racemase_C IPR001608, Ala_racemase_N IPR020622, Ala_racemase_pyridoxalP-BS IPR029066, PLP-binding_barrel |
Pfami | View protein in Pfam PF00842, Ala_racemase_C, 1 hit PF01168, Ala_racemase_N, 1 hit |
PRINTSi | PR00992, ALARACEMASE |
SMARTi | View protein in SMART SM01005, Ala_racemase_C, 1 hit |
SUPFAMi | SSF50621, SSF50621, 1 hit SSF51419, SSF51419, 1 hit |
TIGRFAMsi | TIGR00492, alr, 1 hit |
PROSITEi | View protein in PROSITE PS00395, ALANINE_RACEMASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P29012-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRPIQASLD LQALKQNLSI VRQAATHARV WSVVKANAYG HGIERIWSAI
60 70 80 90 100
GATDGFALLN LEEAITLRER GWKGPILMLE GFFHAQDLEI YDQHRLTTCV
110 120 130 140 150
HSNWQLKALQ NARLKAPLDI YLKVNSGMNR LGFQPDRVLT VWQQLRAMAN
160 170 180 190 200
VGEMTLMSHF AEAEHPDGIS GAMARIEQAA EGLECRRSLS NSAATLWHPE
210 220 230 240 250
AHFDWVRPGI ILYGASPSGQ WRDIANTGLR PVMTLSSEII GVQTLKAGER
260 270 280 290 300
VGYGGRYTAR DEQRIGIVAA GYADGYPRHA PTGTPVLVDG VRTMTVGTVS
310 320 330 340 350
MDMLAVDLTP CPQAGIGTPV ELWGKEIKID DVAAAAGTVG YELMCALALR
VPVVTV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 172 | A → R in AAC36881 (PubMed:7906689).Curated | 1 | |
Sequence conflicti | 215 | A → R in AAC36881 (PubMed:7906689).Curated | 1 | |
Sequence conflicti | 281 | P → L in AAC36881 (PubMed:7906689).Curated | 1 | |
Sequence conflicti | 349 | L → V in AAC36881 (PubMed:7906689).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02948 Unassigned DNA Translation: AAC36881.1 U00096 Genomic DNA Translation: AAC74274.1 AP009048 Genomic DNA Translation: BAA36045.1 |
PIRi | C64865, C53383 |
RefSeqi | NP_415708.1, NC_000913.3 WP_000197881.1, NZ_SSZK01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC74274; AAC74274; b1190 BAA36045; BAA36045; BAA36045 |
GeneIDi | 945754 |
KEGGi | ecj:JW1179 eco:b1190 |
PATRICi | fig|1411691.4.peg.1097 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02948 Unassigned DNA Translation: AAC36881.1 U00096 Genomic DNA Translation: AAC74274.1 AP009048 Genomic DNA Translation: BAA36045.1 |
PIRi | C64865, C53383 |
RefSeqi | NP_415708.1, NC_000913.3 WP_000197881.1, NZ_SSZK01000010.1 |
3D structure databases
SMRi | P29012 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260105, 648 interactors |
DIPi | DIP-9395N |
IntActi | P29012, 2 interactors |
STRINGi | 511145.b1190 |
Proteomic databases
jPOSTi | P29012 |
PaxDbi | P29012 |
PRIDEi | P29012 |
Genome annotation databases
EnsemblBacteriai | AAC74274; AAC74274; b1190 BAA36045; BAA36045; BAA36045 |
GeneIDi | 945754 |
KEGGi | ecj:JW1179 eco:b1190 |
PATRICi | fig|1411691.4.peg.1097 |
Organism-specific databases
EchoBASEi | EB1380 |
Phylogenomic databases
eggNOGi | COG0787, Bacteria |
HOGENOMi | CLU_028393_1_0_6 |
InParanoidi | P29012 |
PhylomeDBi | P29012 |
Enzyme and pathway databases
BioCyci | EcoCyc:ALARACECAT-MONOMER MetaCyc:ALARACECAT-MONOMER |
SABIO-RKi | P29012 |
Miscellaneous databases
PROi | PR:P29012 |
Family and domain databases
Gene3Di | 2.40.37.10, 1 hit 3.20.20.10, 1 hit |
HAMAPi | MF_01201, Ala_racemase, 1 hit |
InterProi | View protein in InterPro IPR000821, Ala_racemase IPR009006, Ala_racemase/Decarboxylase_C IPR011079, Ala_racemase_C IPR001608, Ala_racemase_N IPR020622, Ala_racemase_pyridoxalP-BS IPR029066, PLP-binding_barrel |
Pfami | View protein in Pfam PF00842, Ala_racemase_C, 1 hit PF01168, Ala_racemase_N, 1 hit |
PRINTSi | PR00992, ALARACEMASE |
SMARTi | View protein in SMART SM01005, Ala_racemase_C, 1 hit |
SUPFAMi | SSF50621, SSF50621, 1 hit SSF51419, SSF51419, 1 hit |
TIGRFAMsi | TIGR00492, alr, 1 hit |
PROSITEi | View protein in PROSITE PS00395, ALANINE_RACEMASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALR2_ECOLI | |
Accessioni | P29012Primary (citable) accession number: P29012 Secondary accession number(s): O87498, P78246 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families