Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hematopoietic progenitor cell antigen CD34

Gene

CD34

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

GO - Molecular functioni

  • carbohydrate binding Source: HGNC
  • sulfate binding Source: Ensembl
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
SIGNORiP28906

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietic progenitor cell antigen CD34
Alternative name(s):
CD_antigen: CD34
Gene namesi
Name:CD34
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000174059.16
HGNCiHGNC:1662 CD34
MIMi142230 gene
neXtProtiNX_P28906

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 290ExtracellularSequence analysisAdd BLAST259
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21
Topological domaini312 – 385CytoplasmicSequence analysisAdd BLAST74

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi947
OpenTargetsiENSG00000174059
PharmGKBiPA26211

Polymorphism and mutation databases

BioMutaiCD34
DMDMi3183511

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 312 PublicationsAdd BLAST31
ChainiPRO_000002090032 – 385Hematopoietic progenitor cell antigen CD34Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi51N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi126N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi142N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi194N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi249N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei329PhosphotyrosineBy similarity1
Modified residuei339PhosphotyrosineBy similarity1
Modified residuei346PhosphoserineBy similarity1

Post-translational modificationi

Highly glycosylated.
Phosphorylated on serine residues by PKC.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP28906
MaxQBiP28906
PaxDbiP28906
PeptideAtlasiP28906
PRIDEiP28906
ProteomicsDBi54505
54506 [P28906-2]

PTM databases

GlyConnecti1305
iPTMnetiP28906
PhosphoSitePlusiP28906

Expressioni

Tissue specificityi

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Developmental stagei

On early hematopoietic progenitor cells.

Gene expression databases

BgeeiENSG00000174059 Expressed in 166 organ(s), highest expression level in esophagus
CleanExiHS_CD34
ExpressionAtlasiP28906 baseline and differential
GenevisibleiP28906 HS

Organism-specific databases

HPAiCAB000018
HPA036722
HPA036723

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107385, 1 interactor
IntActiP28906, 2 interactors
STRINGi9606.ENSP00000310036

Structurei

3D structure databases

ProteinModelPortaliP28906
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CD34 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPJJ Eukaryota
ENOG410YPR6 LUCA
GeneTreeiENSGT00390000008414
HOVERGENiHBG003461
InParanoidiP28906
KOiK06474
OMAiGMPRGWT
OrthoDBiEOG091G0K1S
PhylomeDBiP28906
TreeFamiTF335795

Family and domain databases

InterProiView protein in InterPro
IPR008083 CD34
IPR013836 CD34/Podocalyxin
PANTHERiPTHR16677 PTHR16677, 1 hit
PfamiView protein in Pfam
PF06365 CD34_antigen, 1 hit
PRINTSiPR01700 CD34ANTIGEN

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Both isoforms are expressed on the cell surface. CD34-T/CD34-F ratio increases with cell differentiation.

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform CD34-F (identifier: P28906-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS
60 70 80 90 100
NVSTNVSYQE TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT
110 120 130 140 150
SVYGNTNSSV QSQTSVISTV FTTPANVSTP ETTLKPSLSP GNVSDLSTTS
160 170 180 190 200
TSLATSPTKP YTSSSPILSD IKAEIKCSGI REVKLTQGIC LEQNKTSSCA
210 220 230 240 250
EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP QCLLLVLANR
260 270 280 290 300
TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL
310 320 330 340 350
LAVLGITGYF LMNRRSWSPT GERLGEDPYY TENGGGQGYS SGPGTSPEAQ
360 370 380
GKASVNRGAQ ENGTGQATSR NGHSARQHVV ADTEL
Length:385
Mass (Da):40,716
Last modified:July 15, 1998 - v2
Checksum:iBC581A8FCFFE2BB3
GO
Isoform CD34-T (identifier: P28906-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     325-328: GEDP → ELEP
     329-385: Missing.

Show »
Length:328
Mass (Da):35,011
Checksum:i7FFBEBC98D4CC61D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5JTA5Q5JTA5_HUMAN
Hematopoietic progenitor cell antig...
CD34
227Annotation score:

Sequence cautioni

The sequence AAA03181 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187Q → E AA sequence (PubMed:2462139).Curated1
Sequence conflicti325 – 327GED → ELE (PubMed:7678811).Curated3
Sequence conflicti361E → K (PubMed:1370171).Curated1
Sequence conflicti361E → K (PubMed:1374051).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050774367A → S. Corresponds to variant dbSNP:rs28362497Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004159325 – 328GEDP → ELEP in isoform CD34-T. 1 Publication4
Alternative sequenceiVSP_004160329 – 385Missing in isoform CD34-T. 1 PublicationAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81104 mRNA Translation: AAA03181.1 Different initiation.
M81945
, M81938, M81939, M81940, M81941, M81942, M81943, M81944 Genomic DNA Translation: AAA03659.1
S53910 mRNA Translation: AAB25222.1
S53911 mRNA Translation: AAB25223.1
AF523361 mRNA Translation: AAM82157.1
AK291529 mRNA Translation: BAF84218.1
AL356275 Genomic DNA No translation available.
CH471100 Genomic DNA Translation: EAW93459.1
BC039146 mRNA Translation: AAH39146.2
CCDSiCCDS31011.1 [P28906-1]
CCDS31012.1 [P28906-2]
PIRiA38078
I67604
RefSeqiNP_001020280.1, NM_001025109.1 [P28906-1]
NP_001764.1, NM_001773.2 [P28906-2]
UniGeneiHs.374990

Genome annotation databases

EnsembliENST00000310833; ENSP00000310036; ENSG00000174059 [P28906-1]
ENST00000356522; ENSP00000348916; ENSG00000174059 [P28906-2]
GeneIDi947
KEGGihsa:947
UCSCiuc001hgw.2 human [P28906-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

CD34 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81104 mRNA Translation: AAA03181.1 Different initiation.
M81945
, M81938, M81939, M81940, M81941, M81942, M81943, M81944 Genomic DNA Translation: AAA03659.1
S53910 mRNA Translation: AAB25222.1
S53911 mRNA Translation: AAB25223.1
AF523361 mRNA Translation: AAM82157.1
AK291529 mRNA Translation: BAF84218.1
AL356275 Genomic DNA No translation available.
CH471100 Genomic DNA Translation: EAW93459.1
BC039146 mRNA Translation: AAH39146.2
CCDSiCCDS31011.1 [P28906-1]
CCDS31012.1 [P28906-2]
PIRiA38078
I67604
RefSeqiNP_001020280.1, NM_001025109.1 [P28906-1]
NP_001764.1, NM_001773.2 [P28906-2]
UniGeneiHs.374990

3D structure databases

ProteinModelPortaliP28906
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107385, 1 interactor
IntActiP28906, 2 interactors
STRINGi9606.ENSP00000310036

PTM databases

GlyConnecti1305
iPTMnetiP28906
PhosphoSitePlusiP28906

Polymorphism and mutation databases

BioMutaiCD34
DMDMi3183511

Proteomic databases

EPDiP28906
MaxQBiP28906
PaxDbiP28906
PeptideAtlasiP28906
PRIDEiP28906
ProteomicsDBi54505
54506 [P28906-2]

Protocols and materials databases

DNASUi947
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310833; ENSP00000310036; ENSG00000174059 [P28906-1]
ENST00000356522; ENSP00000348916; ENSG00000174059 [P28906-2]
GeneIDi947
KEGGihsa:947
UCSCiuc001hgw.2 human [P28906-1]

Organism-specific databases

CTDi947
DisGeNETi947
EuPathDBiHostDB:ENSG00000174059.16
GeneCardsiCD34
HGNCiHGNC:1662 CD34
HPAiCAB000018
HPA036722
HPA036723
MIMi142230 gene
neXtProtiNX_P28906
OpenTargetsiENSG00000174059
PharmGKBiPA26211
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPJJ Eukaryota
ENOG410YPR6 LUCA
GeneTreeiENSGT00390000008414
HOVERGENiHBG003461
InParanoidiP28906
KOiK06474
OMAiGMPRGWT
OrthoDBiEOG091G0K1S
PhylomeDBiP28906
TreeFamiTF335795

Enzyme and pathway databases

ReactomeiR-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
SIGNORiP28906

Miscellaneous databases

ChiTaRSiCD34 human
GeneWikiiCD34
GenomeRNAii947
PROiPR:P28906
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174059 Expressed in 166 organ(s), highest expression level in esophagus
CleanExiHS_CD34
ExpressionAtlasiP28906 baseline and differential
GenevisibleiP28906 HS

Family and domain databases

InterProiView protein in InterPro
IPR008083 CD34
IPR013836 CD34/Podocalyxin
PANTHERiPTHR16677 PTHR16677, 1 hit
PfamiView protein in Pfam
PF06365 CD34_antigen, 1 hit
PRINTSiPR01700 CD34ANTIGEN
ProtoNetiSearch...

Entry informationi

Entry nameiCD34_HUMAN
AccessioniPrimary (citable) accession number: P28906
Secondary accession number(s): A8K664
, Q15970, Q15971, Q5JTA3, Q5JTA4, Q9UJB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 15, 1998
Last modified: November 7, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again