UniProtKB - P28905 (HOLC_ECOLI)
DNA polymerase III subunit chi
holC
Functioni
Catalytic activityi
- EC:2.7.7.7
GO - Molecular functioni
- DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- DNA replication Source: UniProtKB-KW
- positive regulation of DNA-dependent DNA replication initiation Source: EcoliWiki
- response to radiation Source: EcoCyc
Keywordsi
Molecular function | DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11413-MONOMER MetaCyc:EG11413-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:holC Ordered Locus Names:b4259, JW4216 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- DNA polymerase III, clamp loader chi/psi subcomplex Source: EcoliWiki
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105517 | 1 – 147 | DNA polymerase III subunit chiAdd BLAST | 147 |
Proteomic databases
jPOSTi | P28905 |
PaxDbi | P28905 |
PRIDEi | P28905 |
Interactioni
Subunit structurei
The DNA polymerase III holoenzyme complex contains at least 10 different subunits organized into 3 functionally essential subassemblies: the Pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The Pol III core (subunits alpha, epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities (PubMed:2040637). The polymerase is tethered to the template via the dimeric beta sliding clamp processivity factor. The clamp-loading complex (also called gamma complex) assembles the beta sliding clamp onto the primed template and plays a central role in the organization and communication at the replication fork. The clamp-loading complex contains delta, delta', psi and chi, and 3 copies of either or both of two different DnaX proteins, gamma and tau. The DNA replisome complex has a single clamp loader (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase (PubMed:20413500, PubMed:22157955). The clamp loader hydrolyzes ATP to assemble the beta processivity factor onto the primed template (PubMed:2040637) and plays a central role in the organization and communication at the replication fork.
3 PublicationsBinary interactionsi
Hide detailsP28905
With | #Exp. | IntAct |
---|---|---|
dnaX [P06710] | 20 | EBI-549169,EBI-549140 |
holB [P28631] | 6 | EBI-549169,EBI-549161 |
holD [P28632] | 25 | EBI-549169,EBI-549176 |
ssb [P0AGE0] | 10 | EBI-549169,EBI-1118620 |
Protein-protein interaction databases
BioGRIDi | 4262725, 159 interactors 853072, 5 interactors |
ComplexPortali | CPX-1926, DNA polymerase III clamp loader complex |
DIPi | DIP-9933N |
IntActi | P28905, 41 interactors |
MINTi | P28905 |
STRINGi | 511145.b4259 |
Structurei
Secondary structure
3D structure databases
SMRi | P28905 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28905 |
Family & Domainsi
Phylogenomic databases
eggNOGi | COG2927, Bacteria |
HOGENOMi | CLU_131584_0_0_6 |
InParanoidi | P28905 |
Family and domain databases
Gene3Di | 3.40.50.10110, 1 hit |
InterProi | View protein in InterPro IPR007459, DNA_pol3_chi IPR036768, PolIII_chi_sf |
PANTHERi | PTHR38767, PTHR38767, 1 hit |
Pfami | View protein in Pfam PF04364, DNA_pol3_chi, 1 hit |
SUPFAMi | SSF102400, SSF102400, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKNATFYLLD NDTTVDGLSA VEQLVCEIAA ERWRSGKRVL IACEDEKQAY
60 70 80 90 100
RLDEALWARP AESFVPHNLA GEGPRGGAPV EIAWPQKRSS SRRDILISLR
110 120 130 140
TSFADFATAF TEVVDFVPYE DSLKQLARER YKAYRVAGFN LNTATWK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04574 Genomic DNA Translation: AAA70368.1 Z14155 Genomic DNA Translation: CAA78524.1 U14003 Genomic DNA Translation: AAA97156.1 U00096 Genomic DNA Translation: AAC77216.1 AP009048 Genomic DNA Translation: BAE78256.1 X15130 Genomic DNA Translation: CAA33226.1 |
PIRi | A46739 |
RefSeqi | NP_418680.1, NC_000913.3 WP_000786400.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77216; AAC77216; b4259 BAE78256; BAE78256; BAE78256 |
GeneIDi | 948787 |
KEGGi | ecj:JW4216 eco:b4259 |
PATRICi | fig|1411691.4.peg.2445 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04574 Genomic DNA Translation: AAA70368.1 Z14155 Genomic DNA Translation: CAA78524.1 U14003 Genomic DNA Translation: AAA97156.1 U00096 Genomic DNA Translation: AAC77216.1 AP009048 Genomic DNA Translation: BAE78256.1 X15130 Genomic DNA Translation: CAA33226.1 |
PIRi | A46739 |
RefSeqi | NP_418680.1, NC_000913.3 WP_000786400.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EM8 | X-ray | 2.10 | A/C | 1-147 | [»] | |
3SXU | X-ray | 1.85 | A | 1-147 | [»] | |
SMRi | P28905 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262725, 159 interactors 853072, 5 interactors |
ComplexPortali | CPX-1926, DNA polymerase III clamp loader complex |
DIPi | DIP-9933N |
IntActi | P28905, 41 interactors |
MINTi | P28905 |
STRINGi | 511145.b4259 |
Proteomic databases
jPOSTi | P28905 |
PaxDbi | P28905 |
PRIDEi | P28905 |
Genome annotation databases
EnsemblBacteriai | AAC77216; AAC77216; b4259 BAE78256; BAE78256; BAE78256 |
GeneIDi | 948787 |
KEGGi | ecj:JW4216 eco:b4259 |
PATRICi | fig|1411691.4.peg.2445 |
Organism-specific databases
EchoBASEi | EB1385 |
Phylogenomic databases
eggNOGi | COG2927, Bacteria |
HOGENOMi | CLU_131584_0_0_6 |
InParanoidi | P28905 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11413-MONOMER MetaCyc:EG11413-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P28905 |
PROi | PR:P28905 |
Family and domain databases
Gene3Di | 3.40.50.10110, 1 hit |
InterProi | View protein in InterPro IPR007459, DNA_pol3_chi IPR036768, PolIII_chi_sf |
PANTHERi | PTHR38767, PTHR38767, 1 hit |
Pfami | View protein in Pfam PF04364, DNA_pol3_chi, 1 hit |
SUPFAMi | SSF102400, SSF102400, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HOLC_ECOLI | |
Accessioni | P28905Primary (citable) accession number: P28905 Secondary accession number(s): P11649, Q2M650 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references