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UniProtKB - P28862 (MMP3_MOUSE)
Protein
Stromelysin-1
Gene
Mmp3
Organism
Mus musculus (Mouse)
Status
Functioni
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Catalytic activityi
- Preferential cleavage where P1', P2' and P3' are hydrophobic residues. EC:3.4.24.17
Cofactori
Protein has several cofactor binding sites:- Ca2+By similarityNote: Binds 4 Ca2+ ions per subunit.By similarity
- Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 92 | Zinc 2; in inhibited formBy similarity | 1 | |
Metal bindingi | 124 | Calcium 1By similarity | 1 | |
Metal bindingi | 158 | Calcium 2By similarity | 1 | |
Metal bindingi | 168 | Zinc 1By similarity | 1 | |
Metal bindingi | 170 | Zinc 1By similarity | 1 | |
Metal bindingi | 175 | Calcium 3By similarity | 1 | |
Metal bindingi | 176 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 178 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 180 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 183 | Zinc 1By similarity | 1 | |
Metal bindingi | 190 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 192 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 194 | Calcium 2By similarity | 1 | |
Metal bindingi | 196 | Zinc 1By similarity | 1 | |
Metal bindingi | 198 | Calcium 3By similarity | 1 | |
Metal bindingi | 199 | Calcium 1By similarity | 1 | |
Metal bindingi | 201 | Calcium 1By similarity | 1 | |
Metal bindingi | 201 | Calcium 3By similarity | 1 | |
Metal bindingi | 218 | Zinc 2; catalyticBy similarity | 1 | |
Active sitei | 219 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 222 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 228 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 297 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 389 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 438 | Calcium 4; via carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- endopeptidase activity Source: MGI
- metalloendopeptidase activity Source: GO_Central
- metallopeptidase activity Source: MGI
- peptidase activity Source: UniProtKB
- protein-containing complex binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- cellular response to amino acid stimulus Source: MGI
- cellular response to UV-A Source: UniProtKB
- collagen catabolic process Source: GO_Central
- extracellular matrix organization Source: GO_Central
- negative regulation of hydrogen peroxide metabolic process Source: MGI
- negative regulation of protein kinase B signaling Source: ParkinsonsUK-UCL
- positive regulation of cell migration Source: MGI
- positive regulation of oxidative stress-induced cell death Source: ParkinsonsUK-UCL
- positive regulation of protein-containing complex assembly Source: MGI
- protein catabolic process Source: ParkinsonsUK-UCL
- proteolysis Source: ParkinsonsUK-UCL
- regulation of cell migration Source: MGI
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Collagen degradation |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.24.17, 3474 |
Reactomei | R-MMU-1442490, Collagen degradation R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1592389, Activation of Matrix Metalloproteinases R-MMU-2022090, Assembly of collagen fibrils and other multimeric structures R-MMU-2179392, EGFR Transactivation by Gastrin R-MMU-9009391, Extra-nuclear estrogen signaling |
Protein family/group databases
MEROPSi | M10.005 |
Names & Taxonomyi
Protein namesi | Recommended name: Stromelysin-1 (EC:3.4.24.17)Short name: SL-1 Alternative name(s): EMS-2 Matrix metalloproteinase-3 Short name: MMP-3 Transin-1 |
Gene namesi | Name:Mmp3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97010, Mmp3 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix Curated
Cytosol
- cytosol Source: ParkinsonsUK-UCL
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: BHF-UCL
Mitochondrion
- mitochondrion Source: ParkinsonsUK-UCL
Nucleus
- nucleus Source: MGI
Other locations
- cell body Source: MGI
- dendrite Source: MGI
- extracellular matrix Source: InterPro
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | CuratedAdd BLAST | 17 | |
PropeptideiPRO_0000028730 | 18 – 99 | Activation peptideAdd BLAST | 82 | |
ChainiPRO_0000028731 | 100 – 477 | Stromelysin-1Add BLAST | 378 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 290 ↔ 477 | By similarity |
Keywords - PTMi
Disulfide bond, ZymogenProteomic databases
CPTACi | non-CPTAC-3423 |
MaxQBi | P28862 |
PaxDbi | P28862 |
PeptideAtlasi | P28862 |
PRIDEi | P28862 |
ProteomicsDBi | 295693 |
PTM databases
iPTMneti | P28862 |
PhosphoSitePlusi | P28862 |
Expressioni
Developmental stagei
Present in unfertilized eggs and at the zygote and cleavage stages. Levels increase at the blastocyst stage and with endoderm differentiation.1 Publication
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034497 |
Miscellaneous databases
RNActi | P28862, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 287 – 336 | Hemopexin 1Add BLAST | 50 | |
Repeati | 337 – 383 | Hemopexin 2Add BLAST | 47 | |
Repeati | 385 – 433 | Hemopexin 3Add BLAST | 49 | |
Repeati | 434 – 477 | Hemopexin 4Add BLAST | 44 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 90 – 97 | Cysteine switchBy similarity | 8 |
Domaini
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Sequence similaritiesi
Belongs to the peptidase M10A family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG1565, Eukaryota |
InParanoidi | P28862 |
OrthoDBi | 1075463at2759 |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf |
Pfami | View protein in Pfam PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P28862-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKGLPVLLWL CVVVCSSYPL HDSARDDDAG MELLQKYLEN YYGLAKDVKQ
60 70 80 90 100
FIKKKDSSLI VKKIQEMQKF LGLEMTGKLD SNTMELMHKP RCGVPDVGGF
110 120 130 140 150
STFPGSPKWR KSHITYRIVN YTPDLPRQSV DSAIEKALKV WEEVTPLTFS
160 170 180 190 200
RISEGEADIM ISFAVGEHGD FVPFDGPGTV LAHAYAPGPG INGDAHFDDD
210 220 230 240 250
ERWTEDVTGT NLFLVAAHEL GHSLGLYHSA KAEALMYPVY KSSTDLSRFH
260 270 280 290 300
LSQDDVDGIQ SLYGTPTASP DVLVVPTKSN SLEPETSPMC SSTLFFDAVS
310 320 330 340 350
TLRGEVLFFK DRHFWRKSLR TPEPEFYLIS SFWPSLPSNM DAAYEVTNRD
360 370 380 390 400
TVFIFKGNQF WAIRGHEELA GYPKSIHTLG LPATVKKIDA AISNKEKRKT
410 420 430 440 450
YFFVEDKYWR FDEKKQSMEP GFPRKIAEDF PGVDSRVDAV FEAFGFLYFF
460 470
SGSSQLEFDP NAKKVTHILK SNSWFNC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ922W6 | Q922W6_MOUSE | Matrix metallopeptidase 3 | Mmp3 | 479 | Annotation score: |
Sequence cautioni
The sequence CAA44860 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 468 | I → T (PubMed:2744464).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66402 mRNA Translation: CAA47029.1 X63162 mRNA Translation: CAA44860.1 Different initiation. |
PIRi | JC1476, KCMSS1 |
RefSeqi | NP_034939.1, NM_010809.2 |
Genome annotation databases
GeneIDi | 17392 |
KEGGi | mmu:17392 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66402 mRNA Translation: CAA47029.1 X63162 mRNA Translation: CAA44860.1 Different initiation. |
PIRi | JC1476, KCMSS1 |
RefSeqi | NP_034939.1, NM_010809.2 |
3D structure databases
AlphaFoldDBi | P28862 |
SMRi | P28862 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034497 |
Protein family/group databases
MEROPSi | M10.005 |
PTM databases
iPTMneti | P28862 |
PhosphoSitePlusi | P28862 |
Proteomic databases
CPTACi | non-CPTAC-3423 |
MaxQBi | P28862 |
PaxDbi | P28862 |
PeptideAtlasi | P28862 |
PRIDEi | P28862 |
ProteomicsDBi | 295693 |
Protocols and materials databases
ABCDi | P28862, 1 sequenced antibody |
DNASUi | 17392 |
Genome annotation databases
GeneIDi | 17392 |
KEGGi | mmu:17392 |
Organism-specific databases
CTDi | 4314 |
MGIi | MGI:97010, Mmp3 |
Phylogenomic databases
eggNOGi | KOG1565, Eukaryota |
InParanoidi | P28862 |
OrthoDBi | 1075463at2759 |
Enzyme and pathway databases
BRENDAi | 3.4.24.17, 3474 |
Reactomei | R-MMU-1442490, Collagen degradation R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1592389, Activation of Matrix Metalloproteinases R-MMU-2022090, Assembly of collagen fibrils and other multimeric structures R-MMU-2179392, EGFR Transactivation by Gastrin R-MMU-9009391, Extra-nuclear estrogen signaling |
Miscellaneous databases
BioGRID-ORCSi | 17392, 2 hits in 74 CRISPR screens |
ChiTaRSi | Mmp3, mouse |
PROi | PR:P28862 |
RNActi | P28862, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf |
Pfami | View protein in Pfam PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MMP3_MOUSE | |
Accessioni | P28862Primary (citable) accession number: P28862 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families