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UniProtKB - P28841 (NEC2_RAT)
Protein
Neuroendocrine convertase 2
Gene
Pcsk2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells (By similarity).
By similarityCatalytic activityi
- Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds. EC:3.4.21.94
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 166 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 207 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 383 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- endopeptidase activity Source: RGD
- serine-type endopeptidase activity Source: BHF-UCL
GO - Biological processi
- enkephalin processing Source: RGD
- insulin processing Source: RGD
- islet amyloid polypeptide processing Source: BHF-UCL
- nervous system development Source: RGD
- peptide hormone processing Source: RGD
- protein autoprocessing Source: RGD
- protein processing Source: RGD
- proteolysis Source: RGD
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BRENDAi | 3.4.21.94, 5301 |
Protein family/group databases
MEROPSi | S08.073 |
Names & Taxonomyi
Protein namesi | Recommended name: Neuroendocrine convertase 2 (EC:3.4.21.94)Short name: NEC 2 Alternative name(s): KEX2-like endoprotease 2 Prohormone convertase 2 Proprotein convertase 2 Short name: PC2 |
Gene namesi | Name:Pcsk2 Synonyms:Nec-2, Nec2, Rpc2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3273, Pcsk2 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Other locations
- secretory vesicle By similarity
Note: Localized in the secretion granules.By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB
Nucleus
- nucleoplasm Source: Ensembl
Other locations
- dendrite Source: RGD
- membrane Source: RGD
- neuron projection Source: GO_Central
- neuronal cell body Source: RGD
- perikaryon Source: RGD
- secretory granule lumen Source: Reactome
- transport vesicle Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasmic vesicle, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
PropeptideiPRO_0000027071 | 25 – 108 | Sequence analysisAdd BLAST | 84 | |
ChainiPRO_0000027072 | 109 – 637 | Neuroendocrine convertase 2Add BLAST | 529 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 224 ↔ 375 | By similarity | ||
Disulfide bondi | 316 ↔ 346 | By similarity | ||
Glycosylationi | 374 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 467 ↔ 493 | By similarity | ||
Glycosylationi | 513 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 523 | N-linked (GlcNAc...) asparagineCombined sources | 1 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P28841 |
PTM databases
GlyGeni | P28841, 3 sites, 5 N-linked glycans (1 site) |
iPTMneti | P28841 |
PhosphoSitePlusi | P28841 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000005438, Expressed in frontal cortex and 13 other tissues |
Genevisiblei | P28841, RN |
Interactioni
Binary interactionsi
P28841
With | #Exp. | IntAct |
---|---|---|
Scg5 [P27682] | 4 | EBI-988244,EBI-988232 |
Protein-protein interaction databases
BioGRIDi | 247192, 2 interactors |
IntActi | P28841, 3 interactors |
STRINGi | 10116.ENSRNOP00000007249 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 128 – 452 | Peptidase S8PROSITE-ProRule annotationAdd BLAST | 325 | |
Domaini | 460 – 596 | P/Homo BPROSITE-ProRule annotationAdd BLAST | 137 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3526, Eukaryota |
GeneTreei | ENSGT00940000156965 |
HOGENOMi | CLU_002976_4_4_1 |
InParanoidi | P28841 |
OMAi | PFMTDVI |
OrthoDBi | 308083at2759 |
PhylomeDBi | P28841 |
TreeFami | TF314277 |
Family and domain databases
CDDi | cd04059, Peptidases_S8_Protein_convertases_Kexins_Furin-like, 1 hit |
Gene3Di | 3.30.70.850, 1 hit 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR034182, Kexin/furin IPR002884, P_dom IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR023827, Peptidase_S8_Asp-AS IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel IPR032815, S8_pro-domain IPR038466, S8_pro-domain_sf |
Pfami | View protein in Pfam PF01483, P_proprotein, 1 hit PF00082, Peptidase_S8, 1 hit PF16470, S8_pro-domain, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51829, P_HOMO_B, 1 hit PS51892, SUBTILASE, 1 hit PS00136, SUBTILASE_ASP, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P28841-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEGGCGSQWK AAGLLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA
60 70 80 90 100
EHGFGVRKLP FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ
110 120 130 140 150
EGFDRKKRGY RDINEIDINM NDPLFTKQWY LFNTGQADGT PGLDLNVAEA
160 170 180 190 200
WELGYTGKGV TIGIMDDGID YLHPDLAYNY NSDASYDFSS NDPYPYPRYT
210 220 230 240 250
DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML DQPFMTDIIE
260 270 280 290 300
ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG
310 320 330 340 350
SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL
360 370 380 390 400
ASTFSNGRKR NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN
410 420 430 440 450
VDLTWRDMQH LTVLTSKRNQ LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA
460 470 480 490 500
MVKMAKDWKT VPERFHCVGG SVQNPEKIPP TGKLVLTLQT NACEGKENFV
510 520 530 540 550
RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR DDDSKVGFDK
560 570 580 590 600
WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID
610 620 630
QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 320 | G → GI in AAA41477 (PubMed:1954888).Curated | 1 | |
Sequence conflicti | 342 | Y → H in AAA41477 (PubMed:1954888).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76706 mRNA Translation: AAA40946.1 M83746 mRNA Translation: AAA41477.1 |
PIRi | B41556, KXRTC2 |
RefSeqi | NP_036878.1, NM_012746.1 |
Genome annotation databases
Ensembli | ENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438 |
GeneIDi | 25121 |
KEGGi | rno:25121 |
UCSCi | RGD:3273, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76706 mRNA Translation: AAA40946.1 M83746 mRNA Translation: AAA41477.1 |
PIRi | B41556, KXRTC2 |
RefSeqi | NP_036878.1, NM_012746.1 |
3D structure databases
SMRi | P28841 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247192, 2 interactors |
IntActi | P28841, 3 interactors |
STRINGi | 10116.ENSRNOP00000007249 |
Protein family/group databases
MEROPSi | S08.073 |
PTM databases
GlyGeni | P28841, 3 sites, 5 N-linked glycans (1 site) |
iPTMneti | P28841 |
PhosphoSitePlusi | P28841 |
Proteomic databases
PaxDbi | P28841 |
Genome annotation databases
Ensembli | ENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438 |
GeneIDi | 25121 |
KEGGi | rno:25121 |
UCSCi | RGD:3273, rat |
Organism-specific databases
CTDi | 5126 |
RGDi | 3273, Pcsk2 |
Phylogenomic databases
eggNOGi | KOG3526, Eukaryota |
GeneTreei | ENSGT00940000156965 |
HOGENOMi | CLU_002976_4_4_1 |
InParanoidi | P28841 |
OMAi | PFMTDVI |
OrthoDBi | 308083at2759 |
PhylomeDBi | P28841 |
TreeFami | TF314277 |
Enzyme and pathway databases
BRENDAi | 3.4.21.94, 5301 |
Miscellaneous databases
PROi | PR:P28841 |
Gene expression databases
Bgeei | ENSRNOG00000005438, Expressed in frontal cortex and 13 other tissues |
Genevisiblei | P28841, RN |
Family and domain databases
CDDi | cd04059, Peptidases_S8_Protein_convertases_Kexins_Furin-like, 1 hit |
Gene3Di | 3.30.70.850, 1 hit 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR034182, Kexin/furin IPR002884, P_dom IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR023827, Peptidase_S8_Asp-AS IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel IPR032815, S8_pro-domain IPR038466, S8_pro-domain_sf |
Pfami | View protein in Pfam PF01483, P_proprotein, 1 hit PF00082, Peptidase_S8, 1 hit PF16470, S8_pro-domain, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51829, P_HOMO_B, 1 hit PS51892, SUBTILASE, 1 hit PS00136, SUBTILASE_ASP, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NEC2_RAT | |
Accessioni | P28841Primary (citable) accession number: P28841 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families