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Protein

Cytosol aminopeptidase

Gene

LAP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of N-terminal proline from a peptide.

Cofactori

Note: Binds 2 zinc ions per subunit. One zinc ion is tightly bound and essential for enzyme activity, while the second metal coordination site can be occupied by zinc, magnesium or manganese to give enzymes of different activities.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi282Zinc 2By similarity1
Metal bindingi287Zinc 1By similarity1
Metal bindingi287Zinc 2By similarity1
Active sitei294By similarity1
Metal bindingi305Zinc 2By similarity1
Metal bindingi364Zinc 1By similarity1
Metal bindingi366Zinc 1By similarity1
Metal bindingi366Zinc 2By similarity1
Active sitei368By similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • manganese ion binding Source: InterPro
  • metalloexopeptidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMagnesium, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

SIGNORiP28838

Protein family/group databases

MEROPSiM17.001

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase 3
Short name:
LAP-3
Leucyl aminopeptidase
Peptidase S
Proline aminopeptidase (EC:3.4.11.5)
Prolyl aminopeptidase
Gene namesi
Name:LAP3
Synonyms:LAPEP, PEPS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000002549.12
HGNCiHGNC:18449 LAP3
MIMi170250 gene
neXtProtiNX_P28838

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi51056
OpenTargetsiENSG00000002549
PharmGKBiPA38537

Chemistry databases

ChEMBLiCHEMBL3965
DrugBankiDB07448 (2S)-3-[(R)-[(1S)-1-amino-3-phenylpropyl](hydroxy)phosphoryl]-2-benzylpropanoic acid
DB02386 Leucine Phosphonic Acid

Polymorphism and mutation databases

BioMutaiLAP3
DMDMi124028615

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001658251 – 519Cytosol aminopeptidaseAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42PhosphoserineBy similarity1
Modified residuei45N6-succinyllysineBy similarity1
Modified residuei54PhosphoserineBy similarity1
Modified residuei61N6-succinyllysineBy similarity1
Modified residuei103N6-succinyllysineBy similarity1
Modified residuei180PhosphoserineBy similarity1
Modified residuei194PhosphoserineCombined sources1
Modified residuei221N6-acetyllysine; alternateCombined sources1
Modified residuei221N6-succinyllysine; alternateBy similarity1
Modified residuei238PhosphoserineCombined sources1
Modified residuei455N6-acetyllysine; alternateBy similarity1
Modified residuei455N6-succinyllysine; alternateBy similarity1
Modified residuei476N6-succinyllysineBy similarity1
Modified residuei489N6-acetyllysine; alternateBy similarity1
Modified residuei489N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP28838
MaxQBiP28838
PaxDbiP28838
PeptideAtlasiP28838
PRIDEiP28838
ProteomicsDBi54502
54503 [P28838-2]

2D gel databases

REPRODUCTION-2DPAGEiIPI00789806
P28838
UCD-2DPAGEiP28838

PTM databases

iPTMnetiP28838
PhosphoSitePlusiP28838

Expressioni

Gene expression databases

BgeeiENSG00000002549 Expressed in 229 organ(s), highest expression level in epithelium of bronchus
CleanExiHS_LAP3
ExpressionAtlasiP28838 baseline and differential
GenevisibleiP28838 HS

Organism-specific databases

HPAiHPA029606
HPA029607

Interactioni

Subunit structurei

Homohexamer.

Binary interactionsi

Protein-protein interaction databases

BioGridi119248, 32 interactors
IntActiP28838, 20 interactors
MINTiP28838
STRINGi9606.ENSP00000226299

Chemistry databases

BindingDBiP28838

Structurei

3D structure databases

ProteinModelPortaliP28838
SMRiP28838
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiKOG2597 Eukaryota
COG0260 LUCA
GeneTreeiENSGT00530000063255
HOGENOMiHOG000243129
HOVERGENiHBG003320
InParanoidiP28838
KOiK11142
OMAiMKNTGPR
OrthoDBiEOG091G0ERB
PhylomeDBiP28838
TreeFamiTF314954

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00181 Cytosol_peptidase_M17, 1 hit
InterProiView protein in InterPro
IPR011356 Leucine_aapep/pepB
IPR000819 Peptidase_M17_C
IPR023042 Peptidase_M17_leu_NH2_pept
IPR008283 Peptidase_M17_N
PANTHERiPTHR11963 PTHR11963, 1 hit
PfamiView protein in Pfam
PF00883 Peptidase_M17, 1 hit
PF02789 Peptidase_M17_N, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P28838-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFLLPLPAAG RVVVRRLAVR RFGSRSLSTA DMTKGLVLGI YSKEKEDDVP
60 70 80 90 100
QFTSAGENFD KLLAGKLRET LNISGPPLKA GKTRTFYGLH QDFPSVVLVG
110 120 130 140 150
LGKKAAGIDE QENWHEGKEN IRAAVAAGCR QIQDLELSSV EVDPCGDAQA
160 170 180 190 200
AAEGAVLGLY EYDDLKQKKK MAVSAKLYGS GDQEAWQKGV LFASGQNLAR
210 220 230 240 250
QLMETPANEM TPTRFAEIIE KNLKSASSKT EVHIRPKSWI EEQAMGSFLS
260 270 280 290 300
VAKGSDEPPV FLEIHYKGSP NANEPPLVFV GKGITFDSGG ISIKASANMD
310 320 330 340 350
LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVV
360 370 380 390 400
RAKNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVILNAA TLTGAMDVAL
410 420 430 440 450
GSGATGVFTN SSWLWNKLFE ASIETGDRVW RMPLFEHYTR QVVDCQLADV
460 470 480 490 500
NNIGKYRSAG ACTAAAFLKE FVTHPKWAHL DIAGVMTNKD EVPYLRKGMT
510
GRPTRTLIEF LLRFSQDNA
Length:519
Mass (Da):56,166
Last modified:January 23, 2007 - v3
Checksum:iD960F8F5B9024585
GO
Isoform 2 (identifier: P28838-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Show »
Length:488
Mass (Da):52,771
Checksum:i0CADB3E07FD10B8B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y9Q1H0Y9Q1_HUMAN
Cytosol aminopeptidase
LAP3
208Annotation score:
H0Y983H0Y983_HUMAN
Cytosol aminopeptidase
LAP3
119Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20R → V in AAD17527 (Ref. 1) Curated1
Sequence conflicti22F → S in AAD17527 (Ref. 1) Curated1
Sequence conflicti29T → A in CAG33409 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0226311 – 31Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061738 mRNA Translation: AAD17527.1
CR457128 mRNA Translation: CAG33409.1
AK022055 mRNA Translation: BAG51065.1
AK298613 mRNA Translation: BAG60795.1
BC065564 mRNA Translation: AAH65564.1
BC006199 mRNA Translation: AAH06199.3
CCDSiCCDS3422.1 [P28838-1]
PIRiPT0431
RefSeqiNP_056991.2, NM_015907.2 [P28838-1]
UniGeneiHs.570791

Genome annotation databases

EnsembliENST00000226299; ENSP00000226299; ENSG00000002549 [P28838-1]
ENST00000606142; ENSP00000476028; ENSG00000002549 [P28838-2]
ENST00000618908; ENSP00000481000; ENSG00000002549 [P28838-1]
GeneIDi51056
KEGGihsa:51056
UCSCiuc003gph.1 human [P28838-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061738 mRNA Translation: AAD17527.1
CR457128 mRNA Translation: CAG33409.1
AK022055 mRNA Translation: BAG51065.1
AK298613 mRNA Translation: BAG60795.1
BC065564 mRNA Translation: AAH65564.1
BC006199 mRNA Translation: AAH06199.3
CCDSiCCDS3422.1 [P28838-1]
PIRiPT0431
RefSeqiNP_056991.2, NM_015907.2 [P28838-1]
UniGeneiHs.570791

3D structure databases

ProteinModelPortaliP28838
SMRiP28838
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119248, 32 interactors
IntActiP28838, 20 interactors
MINTiP28838
STRINGi9606.ENSP00000226299

Chemistry databases

BindingDBiP28838
ChEMBLiCHEMBL3965
DrugBankiDB07448 (2S)-3-[(R)-[(1S)-1-amino-3-phenylpropyl](hydroxy)phosphoryl]-2-benzylpropanoic acid
DB02386 Leucine Phosphonic Acid

Protein family/group databases

MEROPSiM17.001

PTM databases

iPTMnetiP28838
PhosphoSitePlusiP28838

Polymorphism and mutation databases

BioMutaiLAP3
DMDMi124028615

2D gel databases

REPRODUCTION-2DPAGEiIPI00789806
P28838
UCD-2DPAGEiP28838

Proteomic databases

EPDiP28838
MaxQBiP28838
PaxDbiP28838
PeptideAtlasiP28838
PRIDEiP28838
ProteomicsDBi54502
54503 [P28838-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000226299; ENSP00000226299; ENSG00000002549 [P28838-1]
ENST00000606142; ENSP00000476028; ENSG00000002549 [P28838-2]
ENST00000618908; ENSP00000481000; ENSG00000002549 [P28838-1]
GeneIDi51056
KEGGihsa:51056
UCSCiuc003gph.1 human [P28838-1]

Organism-specific databases

CTDi51056
DisGeNETi51056
EuPathDBiHostDB:ENSG00000002549.12
GeneCardsiLAP3
H-InvDBiHIX0004121
HGNCiHGNC:18449 LAP3
HPAiHPA029606
HPA029607
MIMi170250 gene
neXtProtiNX_P28838
OpenTargetsiENSG00000002549
PharmGKBiPA38537
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2597 Eukaryota
COG0260 LUCA
GeneTreeiENSGT00530000063255
HOGENOMiHOG000243129
HOVERGENiHBG003320
InParanoidiP28838
KOiK11142
OMAiMKNTGPR
OrthoDBiEOG091G0ERB
PhylomeDBiP28838
TreeFamiTF314954

Enzyme and pathway databases

SIGNORiP28838

Miscellaneous databases

ChiTaRSiLAP3 human
GenomeRNAii51056
PROiPR:P28838
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000002549 Expressed in 229 organ(s), highest expression level in epithelium of bronchus
CleanExiHS_LAP3
ExpressionAtlasiP28838 baseline and differential
GenevisibleiP28838 HS

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00181 Cytosol_peptidase_M17, 1 hit
InterProiView protein in InterPro
IPR011356 Leucine_aapep/pepB
IPR000819 Peptidase_M17_C
IPR023042 Peptidase_M17_leu_NH2_pept
IPR008283 Peptidase_M17_N
PANTHERiPTHR11963 PTHR11963, 1 hit
PfamiView protein in Pfam
PF00883 Peptidase_M17, 1 hit
PF02789 Peptidase_M17_N, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMPL_HUMAN
AccessioniPrimary (citable) accession number: P28838
Secondary accession number(s): B3KMQ3
, Q6IAM6, Q6P0L6, Q9UQE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 184 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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