UniProtKB - P28834 (IDH1_YEAST)
Protein
Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial
Gene
IDH1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Performs an essential role in the oxidative function of the citric acid cycle. Also binds RNA; specifically to the 5'-untranslated leaders of mitochondrial mRNAs.
Miscellaneous
Present with 10500 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.1.1.41
Cofactori
Activity regulationi
Allosterically regulated by several compounds including AMP, NAD+, and citrate.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | SubstrateBy similarity | 1 | |
Binding sitei | 140 | SubstrateBy similarity | 1 | |
Sitei | 194 | Critical for catalysisBy similarity | 1 | |
Metal bindingi | 228 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 228 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
- magnesium ion binding Source: InterPro
- NAD binding Source: InterPro
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- glutamate biosynthetic process Source: SGD
- isocitrate metabolic process Source: SGD
- tricarboxylic acid cycle Source: GO_Central
Keywordsi
Molecular function | Allosteric enzyme, Oxidoreductase, RNA-binding |
Biological process | Tricarboxylic acid cycle |
Ligand | Magnesium, Manganese, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | MetaCyc:YNL037C-MONOMER |
BRENDAi | 1.1.1.41, 984 |
Reactomei | R-SCE-71403, Citric acid cycle (TCA cycle) |
Protein family/group databases
MoonProti | P28834 |
Names & Taxonomyi
Protein namesi | Recommended name: Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial (EC:1.1.1.41)Alternative name(s): Isocitric dehydrogenase NAD(+)-specific ICDH |
Gene namesi | Name:IDH1 Ordered Locus Names:YNL037C ORF Names:N2690 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YNL037C |
SGDi | S000004982, IDH1 |
Subcellular locationi
Mitochondrion
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrial intermembrane space Source: Reactome
- mitochondrial isocitrate dehydrogenase complex (NAD+) Source: SGD
- mitochondrial matrix Source: SGD
- mitochondrial nucleoid Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 11 | Mitochondrion1 PublicationAdd BLAST | 11 | |
ChainiPRO_0000014431 | 12 – 360 | Isocitrate dehydrogenase [NAD] subunit 1, mitochondrialAdd BLAST | 349 |
Proteomic databases
MaxQBi | P28834 |
PaxDbi | P28834 |
PRIDEi | P28834 |
Interactioni
Subunit structurei
Octamer of two non-identical subunits IDH1 and IDH2.
Binary interactionsi
P28834
With | #Exp. | IntAct |
---|---|---|
IDH2 [P28241] | 6 | EBI-8878,EBI-8883 |
Protein-protein interaction databases
BioGRIDi | 35787, 262 interactors |
ComplexPortali | CPX-558, Mitochondrial isocitrate dehydrogenase complex (NAD+) |
DIPi | DIP-4376N |
IntActi | P28834, 26 interactors |
MINTi | P28834 |
STRINGi | 4932.YNL037C |
Miscellaneous databases
RNActi | P28834, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28834 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28834 |
Family & Domainsi
Sequence similaritiesi
Belongs to the isocitrate and isopropylmalate dehydrogenases family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0784, Eukaryota |
GeneTreei | ENSGT00950000182989 |
HOGENOMi | CLU_031953_0_0_1 |
InParanoidi | P28834 |
OMAi | TSMQLVA |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004434, Isocitrate_DH_NAD IPR024084, IsoPropMal-DH-like_dom |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00175, mito_nad_idh, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P28834-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNRTIAKRT LATAAQAERT LPKKYGGRFT VTLIPGDGVG KEITDSVRTI
60 70 80 90 100
FEAENIPIDW ETINIKQTDH KEGVYEAVES LKRNKIGLKG LWHTPADQTG
110 120 130 140 150
HGSLNVALRK QLDIYANVAL FKSLKGVKTR IPDIDLIVIR ENTEGEFSGL
160 170 180 190 200
EHESVPGVVE SLKVMTRPKT ERIARFAFDF AKKYNRKSVT AVHKANIMKL
210 220 230 240 250
GDGLFRNIIT EIGQKEYPDI DVSSIIVDNA SMQAVAKPHQ FDVLVTPSMY
260 270 280 290 300
GTILGNIGAA LIGGPGLVAG ANFGRDYAVF EPGSRHVGLD IKGQNVANPT
310 320 330 340 350
AMILSSTLML NHLGLNEYAT RISKAVHETI AEGKHTTRDI GGSSSTTDFT
360
NEIINKLSTM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95203 Genomic DNA Translation: AAA34711.1 Z71313 Genomic DNA Translation: CAA95904.1 BK006947 Genomic DNA Translation: DAA10508.1 |
PIRi | S31264 |
RefSeqi | NP_014361.1, NM_001182876.1 |
Genome annotation databases
EnsemblFungii | YNL037C_mRNA; YNL037C; YNL037C |
GeneIDi | 855691 |
KEGGi | sce:YNL037C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95203 Genomic DNA Translation: AAA34711.1 Z71313 Genomic DNA Translation: CAA95904.1 BK006947 Genomic DNA Translation: DAA10508.1 |
PIRi | S31264 |
RefSeqi | NP_014361.1, NM_001182876.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3BLV | X-ray | 3.20 | A/C/E/G | 12-360 | [»] | |
3BLW | X-ray | 4.30 | A/C/E/G/I/K/M/O | 12-360 | [»] | |
3BLX | X-ray | 2.70 | A/C/E/G/I/K/M/O | 12-360 | [»] | |
SMRi | P28834 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35787, 262 interactors |
ComplexPortali | CPX-558, Mitochondrial isocitrate dehydrogenase complex (NAD+) |
DIPi | DIP-4376N |
IntActi | P28834, 26 interactors |
MINTi | P28834 |
STRINGi | 4932.YNL037C |
Protein family/group databases
MoonProti | P28834 |
Proteomic databases
MaxQBi | P28834 |
PaxDbi | P28834 |
PRIDEi | P28834 |
Genome annotation databases
EnsemblFungii | YNL037C_mRNA; YNL037C; YNL037C |
GeneIDi | 855691 |
KEGGi | sce:YNL037C |
Organism-specific databases
EuPathDBi | FungiDB:YNL037C |
SGDi | S000004982, IDH1 |
Phylogenomic databases
eggNOGi | KOG0784, Eukaryota |
GeneTreei | ENSGT00950000182989 |
HOGENOMi | CLU_031953_0_0_1 |
InParanoidi | P28834 |
OMAi | TSMQLVA |
Enzyme and pathway databases
BioCyci | MetaCyc:YNL037C-MONOMER |
BRENDAi | 1.1.1.41, 984 |
Reactomei | R-SCE-71403, Citric acid cycle (TCA cycle) |
Miscellaneous databases
EvolutionaryTracei | P28834 |
PROi | PR:P28834 |
RNActi | P28834, protein |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004434, Isocitrate_DH_NAD IPR024084, IsoPropMal-DH-like_dom |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00175, mito_nad_idh, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IDH1_YEAST | |
Accessioni | P28834Primary (citable) accession number: P28834 Secondary accession number(s): D6W1E2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD