Ras-specific guanine nucleotide-releasing factor 1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• GTPase activator activity Source: UniProtKB <p>Traceable Author Statement</p> <p>Used for information from review articles where the original experiments are traceable through that article and also for information from text books or dictionaries.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#tas">GO evidence code guide</a></p> Traceable author statementⁱ
  • "The NMDA receptor is coupled to the ERK pathway by a direct interaction between NR2B and RasGRF1."
    Krapivinsky G., Krapivinsky L., Manasian Y., Ivanov A., Tyzio R., Pellegrino C., Ben-Ari Y., Clapham D.E., Medina I.
    Neuron 40:775-784(2003) [PubMed] [Europe PMC] [Abstract]
• protein C-terminus binding Source: UniProtKB <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "The NMDA receptor is coupled to the ERK pathway by a direct interaction between NR2B and RasGRF1."
    Krapivinsky G., Krapivinsky L., Manasian Y., Ivanov A., Tyzio R., Pellegrino C., Ben-Ari Y., Clapham D.E., Medina I.
    Neuron 40:775-784(2003) [PubMed] [Europe PMC] [Abstract]
• protein kinase binding Source: UniProtKBInferred from physical interactionⁱ
  • Ref.3

    "Requirement for Plk2 in orchestrated ras and rap signaling, homeostatic structural plasticity, and memory."
    Lee K.J., Lee Y., Rozeboom A., Lee J.Y., Udagawa N., Hoe H.S., Pak D.T.
    Neuron 69:957-973(2011) [PubMed] [Europe PMC] [Abstract]

    Cited for: PHOSPHORYLATION AT SER-71; SER-575; SER-611; SER-760; SER-781 AND SER-854, UBIQUITINATION, MUTAGENESIS OF SER-71 AND SER-575.
• Ras GTPase binding Source: RGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "p75-Ras-GRF1 is a c-Jun/AP-1 target protein: its up regulation results in increased Ras activity and is necessary for c-Jun-induced nonadherent growth of Rat1a cells."
    Leaner V.D., Donninger H., Ellis C.A., Clark G.J., Birrer M.J.
    Mol. Cell. Biol. 25:3324-3337(2005) [PubMed] [Europe PMC] [Abstract]
• receptor tyrosine kinase binding Source: RGDInferred from physical interactionⁱ
  • "Activity-dependent interaction of the intracellular domain of rat trkA with intermediate filament proteins, the beta-6 proteasomal subunit, Ras-GRF1, and the p162 subunit of eIF3."
    MacDonald J.I., Verdi J.M., Meakin S.O.
    J. Mol. Neurosci. 13:141-158(1999) [PubMed] [Europe PMC] [Abstract]
• Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processⁱ

• activation of GTPase activity Source: RGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • Ref.1

    "Molecular cloning of cDNAs encoding a guanine-nucleotide-releasing factor for Ras p21."
    Shou C., Farusworth C.L., Neel B.G., Feig L.A.
    Nature 358:351-354(1992) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
• cellular response to antibiotic Source: RGD <p>Inferred from Expression Pattern</p> <p>Covers cases where the annotation is inferred from the timing or location of expression of a gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iep">GO evidence code guide</a></p> Inferred from expression patternⁱ
  • "Hypoinsulinemia alleviates the GRF1/Ras/Akt anti-apoptotic pathway and induces alterations of mitochondrial ras trafficking in neuronal cells."
    Zhuravliova E., Barbakadze T., Narmania N., Sepashvili M., Mikeladze D.G.
    Neurochem. Res. 34:1076-1082(2009) [PubMed] [Europe PMC] [Abstract]
• cellular response to insulin stimulus Source: RGDInferred from expression patternⁱ
  • "Hypoinsulinemia alleviates the GRF1/Ras/Akt anti-apoptotic pathway and induces alterations of mitochondrial ras trafficking in neuronal cells."
    Zhuravliova E., Barbakadze T., Narmania N., Sepashvili M., Mikeladze D.G.
    Neurochem. Res. 34:1076-1082(2009) [PubMed] [Europe PMC] [Abstract]
• neuron projection development Source: HGNC-UCL <p>Inferred from Sequence or Structural Similarity <br />Used for any analysis based on sequence alignment, structure comparison, or evaluation of sequence features such as composition.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iss">GO evidence code guide</a></p> Inferred from sequence or structural similarityⁱ
  • "SCLIP, a microtubule-destabilizing factor, interacts with RasGRF1 and inhibits its ability to promote Rac activation and neurite outgrowth."
    Baldassa S., Gnesutta N., Fascio U., Sturani E., Zippel R.
    J. Biol. Chem. 282:2333-2345(2007) [PubMed] [Europe PMC] [Abstract]
• positive regulation of fibroblast proliferation Source: RGDInferred from mutant phenotypeⁱ
  • "p75-Ras-GRF1 is a c-Jun/AP-1 target protein: its up regulation results in increased Ras activity and is necessary for c-Jun-induced nonadherent growth of Rat1a cells."
    Leaner V.D., Donninger H., Ellis C.A., Clark G.J., Birrer M.J.
    Mol. Cell. Biol. 25:3324-3337(2005) [PubMed] [Europe PMC] [Abstract]
• positive regulation of GTPase activity Source: RGDInferred from mutant phenotypeⁱ
  • "p75-Ras-GRF1 is a c-Jun/AP-1 target protein: its up regulation results in increased Ras activity and is necessary for c-Jun-induced nonadherent growth of Rat1a cells."
    Leaner V.D., Donninger H., Ellis C.A., Clark G.J., Birrer M.J.
    Mol. Cell. Biol. 25:3324-3337(2005) [PubMed] [Europe PMC] [Abstract]
• positive regulation of Ras protein signal transduction Source: UniProtKBInferred from direct assayⁱ
  • Ref.3

    "Requirement for Plk2 in orchestrated ras and rap signaling, homeostatic structural plasticity, and memory."
    Lee K.J., Lee Y., Rozeboom A., Lee J.Y., Udagawa N., Hoe H.S., Pak D.T.
    Neuron 69:957-973(2011) [PubMed] [Europe PMC] [Abstract]

    Cited for: PHOSPHORYLATION AT SER-71; SER-575; SER-611; SER-760; SER-781 AND SER-854, UBIQUITINATION, MUTAGENESIS OF SER-71 AND SER-575.
• regulation of Rac protein signal transduction Source: HGNC-UCLInferred from sequence or structural similarityⁱ
  • "SCLIP, a microtubule-destabilizing factor, interacts with RasGRF1 and inhibits its ability to promote Rac activation and neurite outgrowth."
    Baldassa S., Gnesutta N., Fascio U., Sturani E., Zippel R.
    J. Biol. Chem. 282:2333-2345(2007) [PubMed] [Europe PMC] [Abstract]
• regulation of Ras protein signal transduction Source: HGNC-UCLInferred from sequence or structural similarityⁱ
  • "SCLIP, a microtubule-destabilizing factor, interacts with RasGRF1 and inhibits its ability to promote Rac activation and neurite outgrowth."
    Baldassa S., Gnesutta N., Fascio U., Sturani E., Zippel R.
    J. Biol. Chem. 282:2333-2345(2007) [PubMed] [Europe PMC] [Abstract]
• regulation of Rho protein signal transduction Source: InterPro
• regulation of synaptic plasticity Source: UniProtKBInferred from direct assayⁱ
  • Ref.3

    "Requirement for Plk2 in orchestrated ras and rap signaling, homeostatic structural plasticity, and memory."
    Lee K.J., Lee Y., Rozeboom A., Lee J.Y., Udagawa N., Hoe H.S., Pak D.T.
    Neuron 69:957-973(2011) [PubMed] [Europe PMC] [Abstract]

    Cited for: PHOSPHORYLATION AT SER-71; SER-575; SER-611; SER-760; SER-781 AND SER-854, UBIQUITINATION, MUTAGENESIS OF SER-71 AND SER-575.
• response to amphetamine Source: RGDInferred from expression patternⁱ
  • "Amphetamine alters Ras-guanine nucleotide-releasing factor expression in the rat striatum in vivo."
    Parelkar N.K., Jiang Q., Chu X.P., Guo M.L., Mao L.M., Wang J.Q.
    Eur. J. Pharmacol. 619:50-56(2009) [PubMed] [Europe PMC] [Abstract]
• small GTPase mediated signal transduction Source: InterPro

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationⁱ

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

GO - Molecular functionⁱ

• protein C-terminus binding Source: UniProtKBInferred from physical interactionⁱ
  • "The NMDA receptor is coupled to the ERK pathway by a direct interaction between NR2B and RasGRF1."
    Krapivinsky G., Krapivinsky L., Manasian Y., Ivanov A., Tyzio R., Pellegrino C., Ben-Ari Y., Clapham D.E., Medina I.
    Neuron 40:775-784(2003) [PubMed] [Europe PMC] [Abstract]
• protein kinase binding Source: UniProtKBInferred from physical interactionⁱ
  • Ref.3

    "Requirement for Plk2 in orchestrated ras and rap signaling, homeostatic structural plasticity, and memory."
    Lee K.J., Lee Y., Rozeboom A., Lee J.Y., Udagawa N., Hoe H.S., Pak D.T.
    Neuron 69:957-973(2011) [PubMed] [Europe PMC] [Abstract]

    Cited for: PHOSPHORYLATION AT SER-71; SER-575; SER-611; SER-760; SER-781 AND SER-854, UBIQUITINATION, MUTAGENESIS OF SER-71 AND SER-575.
• Ras GTPase binding Source: RGDInferred from direct assayⁱ
  • "p75-Ras-GRF1 is a c-Jun/AP-1 target protein: its up regulation results in increased Ras activity and is necessary for c-Jun-induced nonadherent growth of Rat1a cells."
    Leaner V.D., Donninger H., Ellis C.A., Clark G.J., Birrer M.J.
    Mol. Cell. Biol. 25:3324-3337(2005) [PubMed] [Europe PMC] [Abstract]
• receptor tyrosine kinase binding Source: RGDInferred from physical interactionⁱ
  • "Activity-dependent interaction of the intracellular domain of rat trkA with intermediate filament proteins, the beta-6 proteasomal subunit, Ras-GRF1, and the p162 subunit of eIF3."
    MacDonald J.I., Verdi J.M., Meakin S.O.
    J. Mol. Neurosci. 13:141-158(1999) [PubMed] [Europe PMC] [Abstract]
• Rho guanyl-nucleotide exchange factor activity Source: InterPro

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)ⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

        10         20         30         40         50
MQKAIRLNDG HVVSLGLLAQ RDGTRKGYLS KRSSDNPKWQ TKWFALLQNL 
        60         70         80         90        100
LFYFESDSSS RPSGLYLLEG SICKRMPSPK RGTSSKESDK QHHYFTVNFS 
       110        120        130        140        150
NDSQKSLELR TDDSKDCDEW VAAIARASYK ILATEHEALM QKYLHLLQVV 
       160        170        180        190        200
ETEKTVAKQL RQQLEDGEVE IERLKAEIAN LIKDNERIQS NQLVAPEDED 
       210        220        230        240        250
SDIKKIKKVQ SFLRGWLCRR KWKNIIQDYI RSPHADSMRK RNQVVFSMLE 
       260        270        280        290        300
AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH 
       310        320        330        340        350
QIFYQGLKAR IASWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC 
       360        370        380        390        400
KQNRDFDKLL KQYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP 
       410        420        430        440        450
HEHVERNSLD YAKSKLEELS RVMHDEVSET ENIRKNLAIE RMITEGCEIL 
       460        470        480        490        500
LDTSQTFVRQ GSLIQVPMSE KGKINKGRLG SLSLKKEGER QCFLFSKHLI 
       510        520        530        540        550
ICTRGSGSKL HLTKNGVISL IDCTLLDDPE NMDDDGKGQE VDHLDFKIWV 
       560        570        580        590        600
EPKDSPPFTV ILVASSRQEK AAWTSDIIQC VDNIRCNGLM MNAFEENSKV 
       610        620        630        640        650
TVPQMIKSDA SLYCDDVDIR FSKTMNSCKV LQIRYASVER LLERLTDLRF 
       660        670        680        690        700
LSIDFLNTFL HSYRVFTDAV VVLDKLISIY KKPITAIPAR SLELLFSSSH 
       710        720        730        740        750
NTKLLYGDAP KSPRASRKFS SPPPLAIGTS SPVRRRKLSL NIPIITGGKA 
       760        770        780        790        800
LELASLGCPS DGYTNIHSPI SPFGKTTLDT SKLCVASSLT RTPEEIDMTT 
       810        820        830        840        850
LEESSGFRKP TSDILKEESD DDQSDVDDTE VSPPTPKSFR NRITQEFPLF 
       860        870        880        890        900
NYNSGIMMTC RDLMDSNRSP LSATSAFAIA TAGANESPAN KEIYRRMSLA 
       910        920        930        940        950
NTGYSSDQRN IDKEFVIRRA ATNRVLNVLR HWVTKHSQDF ETDDLLKYKV 
       960        970        980        990       1000
ICFLEEVMHD PDLLPQERKA AANIMRTLTQ EEITENHSML DELLLMTEGV 
      1010       1020       1030       1040       1050
KTEPFENHSA MEIAEQLTLL DHLVFKSIPY EEFFGQGWMK ADKNERTPYI 
      1060       1070       1080       1090       1100
MKTTRHFNHI SNLIASEILR NEEVSARAST IEKWVAVADI CRCLHNYNAV 
      1110       1120       1130       1140       1150
LEITSSINRS AIFRLKKTWL KVSKQTKSLF DKLQKLVSSD GRFKNLRETL 
      1160       1170       1180       1190       1200
RNCDPPCVPY LGMYLTDLAF LEEGTPNYTE DGLVNFSKMR MISHIIREIR 
      1210       1220       1230       1240 
QFQQTTYKIE PQPKVTQYLV DETFVLDDES LYEASLRIEP KLPT       

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ