UniProtKB - P28738 (KIF5C_MOUSE)
Protein
Kinesin heavy chain isoform 5C
Gene
Kif5c
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in synaptic transmission (By similarity). Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Mediates dendritic trafficking of mRNAs (PubMed:19608740). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 86 – 93 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- apolipoprotein receptor binding Source: MGI
- ATPase activity Source: MGI
- ATP binding Source: UniProtKB-KW
- ATP-dependent microtubule motor activity, plus-end-directed Source: GO_Central
- microtubule binding Source: MGI
- microtubule motor activity Source: GO_Central
GO - Biological processi
- anterograde axonal protein transport Source: UniProtKB
- anterograde dendritic transport of messenger ribonucleoprotein complex Source: MGI
- anterograde dendritic transport of neurotransmitter receptor complex Source: GO_Central
- ATP metabolic process Source: MGI
- axon guidance Source: GO_Central
- cytoskeleton-dependent intracellular transport Source: GO_Central
- dendritic transport of messenger ribonucleoprotein complex Source: MGI
- microtubule-based movement Source: GO_Central
- motor neuron axon guidance Source: MGI
- mRNA transport Source: UniProtKB
- synaptic vesicle transport Source: GO_Central
Keywordsi
Molecular function | Motor protein |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Kinesin heavy chain isoform 5CAlternative name(s): Kinesin heavy chain neuron-specific 2 |
Gene namesi | Name:Kif5c Synonyms:Nkhc2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1098269, Kif5c |
Subcellular locationi
Cytoskeleton
- cytoskeleton Curated
Other locations
- dendrite By similarity
Note: Abundant in distal regions of dendrites.By similarity
Cytoskeleton
- ciliary rootlet Source: MGI
- kinesin complex Source: MGI
- microtubule Source: GO_Central
Other locations
- axon cytoplasm Source: GOC
- axonal growth cone Source: ARUK-UCL
- cytoplasm Source: MGI
- dendrite Source: MGI
- dendrite cytoplasm Source: GOC
- distal axon Source: ARUK-UCL
- neuron projection Source: MGI
- neuronal cell body Source: ARUK-UCL
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoskeleton, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000125356 | 1 – 956 | Kinesin heavy chain isoform 5CAdd BLAST | 956 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 403 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P28738 |
jPOSTi | P28738 |
MaxQBi | P28738 |
PaxDbi | P28738 |
PeptideAtlasi | P28738 |
PRIDEi | P28738 |
PTM databases
iPTMneti | P28738 |
PhosphoSitePlusi | P28738 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026764, Expressed in substantia nigra and 260 other tissues |
ExpressionAtlasi | P28738, baseline and differential |
Genevisiblei | P28738, MM |
Interactioni
Subunit structurei
Binary interactionsi
P28738
With | #Exp. | IntAct |
---|---|---|
Mapk8ip3 [Q9ESN9] | 8 | EBI-2506834,EBI-301496 |
GO - Molecular functioni
- apolipoprotein receptor binding Source: MGI
- microtubule binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200947, 22 interactors |
CORUMi | P28738 |
IntActi | P28738, 17 interactors |
MINTi | P28738 |
STRINGi | 10090.ENSMUSP00000028102 |
Miscellaneous databases
RNActi | P28738, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28738 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28738 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 327 | Kinesin motorPROSITE-ProRule annotationAdd BLAST | 320 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 174 – 315 | Microtubule-bindingAdd BLAST | 142 | |
Regioni | 859 – 956 | GlobularAdd BLAST | 98 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 406 – 923 | Add BLAST | 518 |
Domaini
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Sequence similaritiesi
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0240, Eukaryota |
GeneTreei | ENSGT00940000158539 |
HOGENOMi | CLU_001485_11_1_1 |
InParanoidi | P28738 |
OMAi | AAKQHEM |
OrthoDBi | 1334528at2759 |
TreeFami | TF105225 |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR027640, Kinesin-like_fam IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase |
PANTHERi | PTHR24115, PTHR24115, 1 hit |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P28738-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADPAECSIK VMCRFRPLNE AEILRGDKFI PKFKGEETVV IGQGKPYVFD
60 70 80 90 100
RVLPPNTTQE QVYNACAKQI VKDVLEGYNG TIFAYGQTSS GKTHTMEGKL
110 120 130 140 150
HDPQLMGIIP RIAHDIFDHI YSMDENLEFH IKVSYFEIYL DKIRDLLDVS
160 170 180 190 200
KTNLAVHEDK NRVPYVKGCT ERFVSSPEEV MDVIDEGKAN RHVAVTNMNE
210 220 230 240 250
HSSRSHSIFL INIKQENVET EKKLSGKLYL VDLAGSEKVS KTGAEGAVLD
260 270 280 290 300
EAKNINKSLS ALGNVISALA EGTKTHVPYR DSKMTRILQD SLGGNCRTTI
310 320 330 340 350
VICCSPSVFN EAETKSTLMF GQRAKTIKNT VSVNLELTAE EWKKKYEKEK
360 370 380 390 400
EKNKALKSVL QHLEMELNRW RNGEAVPEDE QISAKDQKSL EPCDNTPIID
410 420 430 440 450
NITPVVDGIS AEKEKYDEEI TSLYRQLDDK DDEINQQSQL AEKLKQQMLD
460 470 480 490 500
QDELLASTRR DYEKIQEELT RLQIENEAAK DEVKEVLQAL EELAVNYDQK
510 520 530 540 550
SQEVEDKTRA NEQLTDELAQ KTTTLTTTQR ELSQLQELSN HQKKRATEIL
560 570 580 590 600
NLLLKDLGEI GGIIGTNDVK TLADVNGVIE EEFTMARLYI SKMKSEVKSL
610 620 630 640 650
VNRSKQLESA QMDSNRKMNA SERELAACQL LISQHEAKIK SLTDYMQNME
660 670 680 690 700
QKRRQLEESQ DSLSEELAKL RAQEKMHEVS FQDKEKEHLT RLQDAEEVKK
710 720 730 740 750
ALEQQMESHR EAHQKQLSRL RDEIEEKQRI IDEIRDLNQK LQLEQERLSS
760 770 780 790 800
DYNKLKIEDQ EREVKLEKLL LLNDKREQAR EDLKGLEETV SRELQTLHNL
810 820 830 840 850
RKLFVQDLTT RVKKSVELDS DDGGGSAAQK QKISFLENNL EQLTKVHKQL
860 870 880 890 900
VRDNADLRCE LPKLEKRLRA TAERVKALES ALKEAKENAM RDRKRYQQEV
910 920 930 940 950
DRIKEAVRAK NMARRAHSAQ IAKPIRPGHY PASSPTAVHA VRGGGGGSSN
STHYQK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2ARD4 | A2ARD4_MOUSE | Kinesin heavy chain isoform 5C | Kif5c | 243 | Annotation score: |
Sequence cautioni
The sequence CAA43677 differs from that shown. Chimeric cDNA. The C-terminus (up to position 300) corresponds to KIF5C sequence.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 387 | Q → H in AAC79804 (PubMed:9782088).Curated | 1 | |
Sequence conflicti | 792 | R → I in AAC79804 (PubMed:9782088).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61435 mRNA Translation: CAA43677.1 Sequence problems. AF067180 mRNA Translation: AAC79804.1 AL845332 Genomic DNA No translation available. AL929069 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL26880.1 BC067051 mRNA Translation: AAH67051.1 |
CCDSi | CCDS16024.1 |
PIRi | S37711 |
RefSeqi | NP_032475.2, NM_008449.2 |
Genome annotation databases
Ensembli | ENSMUST00000028102; ENSMUSP00000028102; ENSMUSG00000026764 |
GeneIDi | 16574 |
KEGGi | mmu:16574 |
UCSCi | uc008jpy.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61435 mRNA Translation: CAA43677.1 Sequence problems. AF067180 mRNA Translation: AAC79804.1 AL845332 Genomic DNA No translation available. AL929069 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL26880.1 BC067051 mRNA Translation: AAH67051.1 |
CCDSi | CCDS16024.1 |
PIRi | S37711 |
RefSeqi | NP_032475.2, NM_008449.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1VFV | X-ray | 1.85 | A | 329-334 | [»] | |
1VFW | X-ray | 2.30 | A | 329-334 | [»] | |
1VFX | X-ray | 2.55 | A | 329-334 | [»] | |
1VFZ | X-ray | 2.24 | A | 329-334 | [»] | |
3J6H | electron microscopy | 8.10 | K | 1-345 | [»] | |
3WRD | X-ray | 2.86 | A/B | 1-334 | [»] | |
3X2T | X-ray | 2.70 | A/B | 1-334 | [»] | |
5HLE | X-ray | 2.90 | A | 10-320 | [»] | |
SMRi | P28738 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200947, 22 interactors |
CORUMi | P28738 |
IntActi | P28738, 17 interactors |
MINTi | P28738 |
STRINGi | 10090.ENSMUSP00000028102 |
PTM databases
iPTMneti | P28738 |
PhosphoSitePlusi | P28738 |
Proteomic databases
EPDi | P28738 |
jPOSTi | P28738 |
MaxQBi | P28738 |
PaxDbi | P28738 |
PeptideAtlasi | P28738 |
PRIDEi | P28738 |
Protocols and materials databases
Antibodypediai | 33631, 151 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028102; ENSMUSP00000028102; ENSMUSG00000026764 |
GeneIDi | 16574 |
KEGGi | mmu:16574 |
UCSCi | uc008jpy.1, mouse |
Organism-specific databases
CTDi | 3800 |
MGIi | MGI:1098269, Kif5c |
Phylogenomic databases
eggNOGi | KOG0240, Eukaryota |
GeneTreei | ENSGT00940000158539 |
HOGENOMi | CLU_001485_11_1_1 |
InParanoidi | P28738 |
OMAi | AAKQHEM |
OrthoDBi | 1334528at2759 |
TreeFami | TF105225 |
Miscellaneous databases
BioGRID-ORCSi | 16574, 0 hits in 17 CRISPR screens |
ChiTaRSi | Kif5c, mouse |
EvolutionaryTracei | P28738 |
PROi | PR:P28738 |
RNActi | P28738, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026764, Expressed in substantia nigra and 260 other tissues |
ExpressionAtlasi | P28738, baseline and differential |
Genevisiblei | P28738, MM |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR027640, Kinesin-like_fam IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase |
PANTHERi | PTHR24115, PTHR24115, 1 hit |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KIF5C_MOUSE | |
Accessioni | P28738Primary (citable) accession number: P28738 Secondary accession number(s): Q6NXI9, Q9Z2F8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 179 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families