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UniProtKB - P28706 (RAD13_SCHPO)
Protein
DNA repair protein rad13
Gene
rad13
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair (Probable).
CuratedCofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 30 | Magnesium 1By similarity | 1 | |
Metal bindingi | 77 | Magnesium 1By similarity | 1 | |
Metal bindingi | 777 | Magnesium 1By similarity | 1 | |
Metal bindingi | 779 | Magnesium 1By similarity | 1 | |
Metal bindingi | 798 | Magnesium 2By similarity | 1 | |
Metal bindingi | 800 | Magnesium 2By similarity | 1 | |
Metal bindingi | 849 | Magnesium 2By similarity | 1 |
GO - Molecular functioni
- endodeoxyribonuclease activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- single-stranded DNA binding Source: GO_Central
- single-stranded DNA endodeoxyribonuclease activity Source: PomBase
GO - Biological processi
- interstrand cross-link repair Source: PomBase
- nucleotide-excision repair Source: PomBase
- nucleotide-excision repair, DNA incision, 3'-to lesion Source: GO_Central
- nucleotide-excision repair involved in interstrand cross-link repair Source: PomBase
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-SPO-5696395, Formation of Incision Complex in GG-NER R-SPO-5696400, Dual Incision in GG-NER R-SPO-6782135, Dual incision in TC-NER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rad13 ORF Names:SPBC3E7.08c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPBC3E7.08c, rad13 |
VEuPathDBi | FungiDB:SPBC3E7.08c |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154036 | 1 – 1112 | DNA repair protein rad13Add BLAST | 1112 |
Proteomic databases
MaxQBi | P28706 |
PaxDbi | P28706 |
PTM databases
iPTMneti | P28706 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 277566, 74 interactors |
STRINGi | 4896.SPBC3E7.08c.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 395 – 414 | UIMPROSITE-ProRule annotationAdd BLAST | 20 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 95 | N-domainAdd BLAST | 95 | |
Regioni | 660 – 681 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 742 – 870 | I-domainAdd BLAST | 129 | |
Regioni | 1056 – 1112 | DisorderedSequence analysisAdd BLAST | 57 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1069 – 1100 | Polar residuesSequence analysisAdd BLAST | 32 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2520, Eukaryota |
HOGENOMi | CLU_003018_0_0_1 |
InParanoidi | P28706 |
OMAi | WTVVQPC |
PhylomeDBi | P28706 |
Family and domain databases
InterProi | View protein in InterPro IPR036279, 5-3_exonuclease_C_sf IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR003903, UIM_dom IPR006086, XPG-I_dom IPR006084, XPG/Rad2 IPR001044, XPG/Rad2_eukaryotes IPR019974, XPG_CS IPR006085, XPG_DNA_repair_N |
Pfami | View protein in Pfam PF00867, XPG_I, 1 hit PF00752, XPG_N, 1 hit |
PRINTSi | PR00853, XPGRADSUPER PR00066, XRODRMPGMNTG |
SMARTi | View protein in SMART SM00279, HhH2, 1 hit SM00484, XPGI, 1 hit SM00485, XPGN, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00600, rad2, 1 hit |
PROSITEi | View protein in PROSITE PS50330, UIM, 1 hit PS00841, XPG_1, 1 hit PS00842, XPG_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P28706-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGVSGLWDIL EPVKRPVKLE TLVNKRLAID ASIWIYQFLK AVRDKEGNQL
60 70 80 90 100
KSSHVVGFFR RICKLLFFGI KPVFVFDGGA PSLKRQTIQK RQARRLDREE
110 120 130 140 150
NATVTANKLL ALQMRHQAML LEENNKKATA LANASVQNER QMPSSMTLDN
160 170 180 190 200
SEIKPVLNQR KNYLKPDPYQ LPEMDVSFDK LGSSYDPRIM SQDELTQYVS
210 220 230 240 250
SFTKIEDINL FDFSNIDFDS ELFQSLPDTD KYSILSAARL RSRLRMGLSS
260 270 280 290 300
EQLSEMFPNR MDFSRFQIER LKERNDLTQR LMDFTGMNEF GPSRVVSEKN
310 320 330 340 350
REYILVKNEG AEGGWALGVI SGSTNNEPII IDDEATKLSS NLIDEDEDEA
360 370 380 390 400
FYDVPLPSRS HSMNPRELVA AKLKEIKENS FSENQQSDEA DYNVTDDLIL
410 420 430 440 450
QLATQQSLEE NKKSKELFSL SASEFDKLNS EKKTFEILST DIPAEDSMNS
460 470 480 490 500
LLNDEENLKL EHVGDVSNDS LAFAEKKHPE NGTSIFMDAL PSASREKKTN
510 520 530 540 550
DLIDPLPFQP MDWGKSIFFE KLKKPTETFM DSKTDIPSEA PDNSKLVEDT
560 570 580 590 600
NLHTINATVN IESDLDAAKP GIENPIISPL LPVKDDEKDL DLRELNPLEP
610 620 630 640 650
FENMKEQADD GTVTNPLNVS SDKAMSVYLL SSENAKDTGD IKSESIDAVL
660 670 680 690 700
PTLETSSPSL SIPTDFQKEA SPNKGAAALS SKVEPEVVEK LLDEEEEEMI
710 720 730 740 750
IRMAEEEKEY DRFVSELNQR HETEEWNQEA FEKRLKELKN QKRSEKRDAD
760 770 780 790 800
EVTQVMIKEC QELLRLFGLP YIVAPQEAEA QCSKLLELKL VDGIVTDDSD
810 820 830 840 850
VFLFGGTRVY RNMFNQNKFV ELYLMDDMKR EFNVNQMDLI KLAHLLGSDY
860 870 880 890 900
TMGLSRVGPV LALEILHEFP GDTGLFEFKK WFQRLSTGHA SKNDVNTPVK
910 920 930 940 950
KRINKLVGKI ILPSEFPNPL VDEAYLHPAV DDSKQSFQWG IPDLDELRQF
960 970 980 990 1000
LMATVGWSKQ RTNEVLLPVI QDMHKKQFVG TQSNLTQFFE GGNTNVYAPR
1010 1020 1030 1040 1050
VAYHFKSKRL ENALSSFKNQ ISNQSPMSEE IQADADAFGE SKGSDELQSR
1060 1070 1080 1090 1100
ILRRKKMMAS KNSSDSDSDS EDNFLASLTP KTNSSSISIE NLPRKTKLST
1110
SLLKKPSKRR RK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 8 | D → N in CAA47291 (PubMed:8464724).Curated | 1 | |
Sequence conflicti | 738 – 743 | LKNQKR → AQKSKKG in CAA47291 (PubMed:8464724).Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66795 mRNA Translation: CAA47291.1 CU329671 Genomic DNA Translation: CAA19011.1 |
PIRi | S30301 T40382 |
RefSeqi | NP_596095.1, NM_001022011.2 |
Genome annotation databases
EnsemblFungii | SPBC3E7.08c.1; SPBC3E7.08c.1:pep; SPBC3E7.08c |
GeneIDi | 2541051 |
KEGGi | spo:SPBC3E7.08c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66795 mRNA Translation: CAA47291.1 CU329671 Genomic DNA Translation: CAA19011.1 |
PIRi | S30301 T40382 |
RefSeqi | NP_596095.1, NM_001022011.2 |
3D structure databases
SMRi | P28706 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 277566, 74 interactors |
STRINGi | 4896.SPBC3E7.08c.1 |
PTM databases
iPTMneti | P28706 |
Proteomic databases
MaxQBi | P28706 |
PaxDbi | P28706 |
Genome annotation databases
EnsemblFungii | SPBC3E7.08c.1; SPBC3E7.08c.1:pep; SPBC3E7.08c |
GeneIDi | 2541051 |
KEGGi | spo:SPBC3E7.08c |
Organism-specific databases
PomBasei | SPBC3E7.08c, rad13 |
VEuPathDBi | FungiDB:SPBC3E7.08c |
Phylogenomic databases
eggNOGi | KOG2520, Eukaryota |
HOGENOMi | CLU_003018_0_0_1 |
InParanoidi | P28706 |
OMAi | WTVVQPC |
PhylomeDBi | P28706 |
Enzyme and pathway databases
Reactomei | R-SPO-5696395, Formation of Incision Complex in GG-NER R-SPO-5696400, Dual Incision in GG-NER R-SPO-6782135, Dual incision in TC-NER |
Miscellaneous databases
PROi | PR:P28706 |
Family and domain databases
InterProi | View protein in InterPro IPR036279, 5-3_exonuclease_C_sf IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR003903, UIM_dom IPR006086, XPG-I_dom IPR006084, XPG/Rad2 IPR001044, XPG/Rad2_eukaryotes IPR019974, XPG_CS IPR006085, XPG_DNA_repair_N |
Pfami | View protein in Pfam PF00867, XPG_I, 1 hit PF00752, XPG_N, 1 hit |
PRINTSi | PR00853, XPGRADSUPER PR00066, XRODRMPGMNTG |
SMARTi | View protein in SMART SM00279, HhH2, 1 hit SM00484, XPGI, 1 hit SM00485, XPGN, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00600, rad2, 1 hit |
PROSITEi | View protein in PROSITE PS50330, UIM, 1 hit PS00841, XPG_1, 1 hit PS00842, XPG_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD13_SCHPO | |
Accessioni | P28706Primary (citable) accession number: P28706 Secondary accession number(s): O59728 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | February 21, 2001 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - SIMILARITY comments
Index of protein domains and families