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Entry version 178 (12 Aug 2020)
Sequence version 2 (01 Mar 2004)
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Protein

Bifunctional glutamate/proline--tRNA ligase

Gene

GluProRS

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei217ATPBy similarity1
Binding sitei404ATPBy similarity1
Binding sitei1353L-prolineBy similarity1
Binding sitei1443L-prolineBy similarity1
Binding sitei1476ATPBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi1648ZincBy similarity1
Metal bindingi1653ZincBy similarity1
Metal bindingi1695ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi438 – 442ATPBy similarity5
Nucleotide bindingi1353 – 1355ATPBy similarity3
Nucleotide bindingi1364 – 1365ATPBy similarity2
Nucleotide bindingi1438 – 1441ATPBy similarity4

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, Multifunctional enzyme, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Bifunctional glutamate/proline--tRNA ligase
Alternative name(s):
Bifunctional aminoacyl-tRNA synthetase
Including the following 2 domains:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase1 Publication
Proline--tRNA ligase (EC:6.1.1.15By similarity)
Alternative name(s):
Prolyl-tRNA synthetase
Short name:
ProRS
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:GluProRS1 PublicationImported
Synonyms:Aats-gluproImported
ORF Names:CG5394Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3R

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0005674, GluProRS

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001197411 – 1714Bifunctional glutamate/proline--tRNA ligaseAdd BLAST1714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1110Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P28668

PRoteomics IDEntifications database

More...
PRIDEi
P28668

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P28668

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0005674, Expressed in eye disc (Drosophila) and 37 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
P28668, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
P28668, DM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.

By similarity

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
67770, 47 interactors

Protein interaction database and analysis system

More...
IntActi
P28668, 10 interactors

STRING: functional protein association networks

More...
STRINGi
7227.FBpp0083898

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P28668

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini744 – 800WHEP-TRS 1Add BLAST57
Domaini816 – 872WHEP-TRS 2Add BLAST57
Domaini890 – 946WHEP-TRS 3Add BLAST57
Domaini969 – 1025WHEP-TRS 4Add BLAST57
Domaini1044 – 1100WHEP-TRS 5Add BLAST57
Domaini1118 – 1174WHEP-TRS 6Add BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni170 – 754Glutamate--tRNA ligaseAdd BLAST585
Regioni1207 – 1714Proline--tRNA ligaseAdd BLAST508
Regioni1322 – 1324L-proline bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi209 – 220'HIGH' regionAdd BLAST12
Motifi438 – 442'KMSKS' region5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1174 – 1180Poly-Gly7

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1147, Eukaryota
KOG4163, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00550000074815

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_001882_0_0_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
P28668

KEGG Orthology (KO)

More...
KOi
K14163

Identification of Orthologs from Complete Genome Data

More...
OMAi
WDPKGNN

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P28668

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00778, ProRS_core_arch_euk, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.40.240.10, 2 hits
3.30.110.30, 1 hit
3.40.50.620, 1 hit
3.40.50.800, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_02076, Glu_tRNA_synth_type2, 1 hit
MF_01571, Pro_tRNA_synth_type3, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002314, aa-tRNA-synt_IIb
IPR001412, aa-tRNA-synth_I_CS
IPR006195, aa-tRNA-synth_II
IPR004154, Anticodon-bd
IPR036621, Anticodon-bd_dom_sf
IPR004526, Glu-tRNA-synth_arc/euk
IPR000924, Glu/Gln-tRNA-synth
IPR020058, Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059, Glu/Gln-tRNA-synth_Ib_codon-bd
IPR036282, Glutathione-S-Trfase_C_sf
IPR004046, GST_C
IPR004499, Pro-tRNA-ligase_IIa_arc-type
IPR016061, Pro-tRNA_ligase_II_C
IPR017449, Pro-tRNA_synth_II
IPR033721, ProRS_core_arch_euk
IPR020056, Rbsml_L25/Gln-tRNA_synth_N
IPR011035, Ribosomal_L25/Gln-tRNA_synth
IPR014729, Rossmann-like_a/b/a_fold
IPR009068, S15_NS1_RNA-bd
IPR000738, WHEP-TRS_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF14497, GST_C_3, 1 hit
PF03129, HGTP_anticodon, 1 hit
PF09180, ProRS-C_1, 1 hit
PF00749, tRNA-synt_1c, 1 hit
PF03950, tRNA-synt_1c_C, 1 hit
PF00587, tRNA-synt_2b, 1 hit
PF00458, WHEP-TRS, 6 hits

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR00987, TRNASYNTHGLU

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00946, ProRS-C_1, 1 hit
SM00991, WHEP-TRS, 6 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47060, SSF47060, 6 hits
SSF47616, SSF47616, 1 hit
SSF50715, SSF50715, 1 hit
SSF64586, SSF64586, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00463, gltX_arch, 1 hit
TIGR00408, proS_fam_I, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00178, AA_TRNA_LIGASE_I, 1 hit
PS50862, AA_TRNA_LIGASE_II, 1 hit
PS00762, WHEP_TRS_1, 6 hits
PS51185, WHEP_TRS_2, 6 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Isoform A (identifier: P28668-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSIKLKANLN NPPISGLATA HLINGTVPVE IVWSKEETSL QFPDNRLLVC
60 70 80 90 100
HSNNDVLRAL ARAAPDYKLY GETAIERTQI DHWLSFSLTC EDDISWALSF
110 120 130 140 150
LDKSIAPVTY LVANKLTIAD FALFNEMHSR YEFLAAKGIP QHVQRWYDLI
160 170 180 190 200
TAQPLIQKVL QSLPEDAKVK RSPQSSKEQT PAKTGERKQE GKFVDLPGAE
210 220 230 240 250
MGKVVVRFPP EASGYLHIGH AKAALLNQYY ALAFQGTLIM RFDDTNPAKE
260 270 280 290 300
TVEFENVILG DLEQLQIKPD VFTHTSNYFD LMLDYCVRLI KESKAYVDDT
310 320 330 340 350
PPEQMKLERE QRVESANRSN SVEKNLSLWE EMVKGSEKGQ KYCVRAKIDM
360 370 380 390 400
SSPNGCMRDP TIYRCKNEPH PRTGTKYKVY PTYDFACPIV DAIENVTHTL
410 420 430 440 450
RTTEYHDRDD QFYWFIDALK LRKPYIWSYS RLNMTNTVLS KRKLTWFVDS
460 470 480 490 500
GLVDGWDDPR FPTVRGIIRR GMTVEGLKEF IIAQGSSKSV VFMNWDKIWA
510 520 530 540 550
FNKKVIDPIA PRYTALEKEK RVIVNVAGAK VERIQVSVHP KDESLGKKTV
560 570 580 590 600
LLGPRIYIDY VDAEALKEGE NATFINWGNI LIRKVNKDAS GNITSVDAAL
610 620 630 640 650
NLENKDFKKT LKLTWLAVED DPSAYPPTFC VYFDNIISKA VLGKDEDFKQ
660 670 680 690 700
FIGHKTRDEV PMLGDPELKK CKKGDIIQLQ RRGFFKVDVA YAPPSGYTNV
710 720 730 740 750
PSPIVLFSIP DGHTKDVPTS GLKVNAPDAK ATKKASSPVS SSGQASELDS
760 770 780 790 800
QISQQGDLVR DLKSKKAAKD QIDVAVKKLL ALKADYKSAT GKDWKPGQTS
810 820 830 840 850
ASSAPVPAAS SSSANDAVSV NASIVKQGDL VRDLKGKKAS KPEIDAAVKT
860 870 880 890 900
LLELKAQYKT LTGQDWKPGT VPTTAAPSAS AAPSVGVNDS VAQILSQITA
910 920 930 940 950
QGDKVRELKS AKADKATVDA AVKTLLSLKA DYKAATGSDW KPGTTAPAPA
960 970 980 990 1000
AAPVKVKQEK NPDPASVLTV NTLLNKIAQQ GDKIRQLKSA KSEKSLVEAE
1010 1020 1030 1040 1050
VKLLLALKTD YKSLTGQEWK PGTVAPAPTT VNVIDLTGGD SGSDVGSVLS
1060 1070 1080 1090 1100
KIQAQGDKIR KLKSEKAAKN VIDPEVKTLL ALKGEYKTLS GKDWTPDAKS
1110 1120 1130 1140 1150
EPAVVKKEAS PVSMASPAKD ELTQEINAQG EKVRAAKGNK AAKEVIDAEV
1160 1170 1180 1190 1200
AKLLALKAKY KEVTGTDFPV AGRGGGGGGG SAKKAPKEAQ PKPAKPVKKE
1210 1220 1230 1240 1250
PAADASGAVK KQTRLGLEAT KEDNLPDWYS QVITKGEMIE YYDVSGCYIL
1260 1270 1280 1290 1300
RQWSFAIWKA IKTWFDAEIT RMGVKECYFP IFVSKAVLEK EKTHIADFAP
1310 1320 1330 1340 1350
EVAWVTKSGD SDLAEPIAVR PTSETVMYPA YAKWVQSYRD LPIRLNQWNN
1360 1370 1380 1390 1400
VVRWEFKQPT PFLRTREFLW QEGHTAFADK EEAAKEVLDI LDLYALVYTH
1410 1420 1430 1440 1450
LLAIPVVKGR KTEKEKFAGG DYTTTVEAFI SASGRAIQGA TSHHLGQNFS
1460 1470 1480 1490 1500
KMFEIVYEDP ETQQKKYVYQ NSWGITTRTI GVMIMVHADN QGLVLPPHVA
1510 1520 1530 1540 1550
CIQAIVVPCG ITVNTKDDER AQLLDACKAL EKRLVGGGVR CEGDYRDNYS
1560 1570 1580 1590 1600
PGWKFNHWEL KGVPLRLEVG PKDLKAQQLV AVRRDTVEKI TIPLADVEKK
1610 1620 1630 1640 1650
IPALLETIHE SMLNKAQEDM TSHTKKVTNW TDFCGFLEQK NILLAPFCGE
1660 1670 1680 1690 1700
ISCEDKIKAD SARGEEAEPG APAMGAKSLC IPFDQPAPIA ASDKCINPSC
1710
TNKPKFYTLF GRSY
Length:1,714
Mass (Da):189,412
Last modified:March 1, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3F8CF3DB128765A8
GO
Isoform B (identifier: P28668-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-718: Missing.
     719-732: TSGLKVNAPDAKAT → MLNYLACGSLSSTS

Show »
Length:996
Mass (Da):107,835
Checksum:i0E0D298DF6D006C9
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAN71400 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti102 – 106DKSIA → TSPLP (PubMed:1756734).Curated5
Sequence conflicti102 – 106DKSIA → TSPLP (PubMed:9063462).Curated5
Sequence conflicti233 – 234AF → VC (PubMed:1756734).Curated2
Sequence conflicti233 – 234AF → VC (PubMed:9063462).Curated2
Sequence conflicti341 – 345KYCVR → NTACA (PubMed:1756734).Curated5
Sequence conflicti341 – 345KYCVR → NTACA (PubMed:9063462).Curated5
Sequence conflicti583R → K (PubMed:1756734).Curated1
Sequence conflicti583R → K (PubMed:9063462).Curated1
Sequence conflicti692A → L (PubMed:1756734).Curated1
Sequence conflicti692A → L (PubMed:9063462).Curated1
Sequence conflicti753S → T (PubMed:1756734).Curated1
Sequence conflicti753S → T (PubMed:9063462).Curated1
Sequence conflicti802S → T (PubMed:1756734).Curated1
Sequence conflicti802S → T (PubMed:9063462).Curated1
Sequence conflicti873T → P (PubMed:1756734).Curated1
Sequence conflicti873T → P (PubMed:9063462).Curated1
Sequence conflicti887V → G (PubMed:1756734).Curated1
Sequence conflicti887V → G (PubMed:9063462).Curated1
Sequence conflicti1035D → G in AAN71400 (PubMed:12537569).Curated1
Sequence conflicti1202Missing (PubMed:1756734).Curated1
Sequence conflicti1202Missing (PubMed:9063462).Curated1
Sequence conflicti1461E → EK (PubMed:1756734).Curated1
Sequence conflicti1461E → EK (PubMed:9063462).Curated1
Sequence conflicti1587V → G (PubMed:1756734).Curated1
Sequence conflicti1587V → G (PubMed:9063462).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0096091 – 718Missing in isoform B. 1 PublicationAdd BLAST718
Alternative sequenceiVSP_009610719 – 732TSGLK…DAKAT → MLNYLACGSLSSTS in isoform B. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
M74104 mRNA Translation: AAA28594.1
U59923 Genomic DNA Translation: AAC47469.1
AE014297 Genomic DNA Translation: AAF56211.1
AE014297 Genomic DNA Translation: AAN13964.1
AY058703 mRNA Translation: AAL13932.1
BT001645 mRNA Translation: AAN71400.1 Different initiation.

Protein sequence database of the Protein Information Resource

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PIRi
S18644

NCBI Reference Sequences

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RefSeqi
NP_524471.2, NM_079747.3 [P28668-1]
NP_732925.1, NM_170103.2 [P28668-2]

Genome annotation databases

Ensembl metazoan genome annotation project

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EnsemblMetazoai
FBtr0084511; FBpp0083898; FBgn0005674 [P28668-1]
FBtr0084512; FBpp0083899; FBgn0005674 [P28668-2]

Database of genes from NCBI RefSeq genomes

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GeneIDi
42834

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
dme:Dmel_CG5394

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74104 mRNA Translation: AAA28594.1
U59923 Genomic DNA Translation: AAC47469.1
AE014297 Genomic DNA Translation: AAF56211.1
AE014297 Genomic DNA Translation: AAN13964.1
AY058703 mRNA Translation: AAL13932.1
BT001645 mRNA Translation: AAN71400.1 Different initiation.
PIRiS18644
RefSeqiNP_524471.2, NM_079747.3 [P28668-1]
NP_732925.1, NM_170103.2 [P28668-2]

3D structure databases

SMRiP28668
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi67770, 47 interactors
IntActiP28668, 10 interactors
STRINGi7227.FBpp0083898

PTM databases

iPTMnetiP28668

Proteomic databases

PaxDbiP28668
PRIDEiP28668

Genome annotation databases

EnsemblMetazoaiFBtr0084511; FBpp0083898; FBgn0005674 [P28668-1]
FBtr0084512; FBpp0083899; FBgn0005674 [P28668-2]
GeneIDi42834
KEGGidme:Dmel_CG5394

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
42834
FlyBaseiFBgn0005674, GluProRS

Phylogenomic databases

eggNOGiKOG1147, Eukaryota
KOG4163, Eukaryota
GeneTreeiENSGT00550000074815
HOGENOMiCLU_001882_0_0_1
InParanoidiP28668
KOiK14163
OMAiWDPKGNN
PhylomeDBiP28668

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

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BioGRID-ORCSi
42834, 0 hits in 5 CRISPR screens

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

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GenomeRNAii
42834

Protein Ontology

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PROi
PR:P28668

Gene expression databases

BgeeiFBgn0005674, Expressed in eye disc (Drosophila) and 37 other tissues
ExpressionAtlasiP28668, baseline and differential
GenevisibleiP28668, DM

Family and domain databases

CDDicd00778, ProRS_core_arch_euk, 1 hit
Gene3Di2.40.240.10, 2 hits
3.30.110.30, 1 hit
3.40.50.620, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_02076, Glu_tRNA_synth_type2, 1 hit
MF_01571, Pro_tRNA_synth_type3, 1 hit
InterProiView protein in InterPro
IPR002314, aa-tRNA-synt_IIb
IPR001412, aa-tRNA-synth_I_CS
IPR006195, aa-tRNA-synth_II
IPR004154, Anticodon-bd
IPR036621, Anticodon-bd_dom_sf
IPR004526, Glu-tRNA-synth_arc/euk
IPR000924, Glu/Gln-tRNA-synth
IPR020058, Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059, Glu/Gln-tRNA-synth_Ib_codon-bd
IPR036282, Glutathione-S-Trfase_C_sf
IPR004046, GST_C
IPR004499, Pro-tRNA-ligase_IIa_arc-type
IPR016061, Pro-tRNA_ligase_II_C
IPR017449, Pro-tRNA_synth_II
IPR033721, ProRS_core_arch_euk
IPR020056, Rbsml_L25/Gln-tRNA_synth_N
IPR011035, Ribosomal_L25/Gln-tRNA_synth
IPR014729, Rossmann-like_a/b/a_fold
IPR009068, S15_NS1_RNA-bd
IPR000738, WHEP-TRS_dom
PfamiView protein in Pfam
PF14497, GST_C_3, 1 hit
PF03129, HGTP_anticodon, 1 hit
PF09180, ProRS-C_1, 1 hit
PF00749, tRNA-synt_1c, 1 hit
PF03950, tRNA-synt_1c_C, 1 hit
PF00587, tRNA-synt_2b, 1 hit
PF00458, WHEP-TRS, 6 hits
PRINTSiPR00987, TRNASYNTHGLU
SMARTiView protein in SMART
SM00946, ProRS-C_1, 1 hit
SM00991, WHEP-TRS, 6 hits
SUPFAMiSSF47060, SSF47060, 6 hits
SSF47616, SSF47616, 1 hit
SSF50715, SSF50715, 1 hit
SSF64586, SSF64586, 1 hit
TIGRFAMsiTIGR00463, gltX_arch, 1 hit
TIGR00408, proS_fam_I, 1 hit
PROSITEiView protein in PROSITE
PS00178, AA_TRNA_LIGASE_I, 1 hit
PS50862, AA_TRNA_LIGASE_II, 1 hit
PS00762, WHEP_TRS_1, 6 hits
PS51185, WHEP_TRS_2, 6 hits

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYEP_DROME
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P28668
Secondary accession number(s): Q8IGR4
, Q8IMX9, Q95TL3, Q9VCF5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: March 1, 2004
Last modified: August 12, 2020
This is version 178 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. SIMILARITY comments
    Index of protein domains and families
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