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Protein

MARCKS-related protein

Gene

Marcksl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the control of cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation (PubMed:22751924). When unphosphorylated, induces cell migration (PubMed:22751924). When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration (PubMed:22751924). May be involved in coupling the protein kinase C and calmodulin signal transduction systems (Probable).1 Publication1 Publication

GO - Molecular functioni

GO - Biological processi

  • positive regulation of cell proliferation Source: MGI

Keywordsi

Molecular functionActin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MARCKS-related protein
Alternative name(s):
Brain protein F52
MARCKS-like protein 1
Macrophage myristoylated alanine-rich C kinase substrate
Short name:
Mac-MARCKS
Short name:
MacMARCKS
Gene namesi
Name:Marcksl1
Synonyms:Mlp, Mrp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:97143 Marcksl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120S → A: Complete loss of MAPK-mediated phosphorylation; when associated with A-148 and A-183. In neurons, induces the generation of large, flat lamellipodial protrusions; when associated with A-148 and A-183. 1 Publication1
Mutagenesisi120S → D: Induction of F-actin-bundling; when associated with D-148 and D-183. In neurons, induces the generation of long and prominent filopodia; when associated with D-148 and D-183. 1 Publication1
Mutagenesisi148T → A: Complete loss of MAPK-mediated phosphorylation; when associated with A-120 and A-183. In neurons, induces the generation of large, flat lamellipodial protrusions; when associated with A-120 and A-183. 1 Publication1
Mutagenesisi148T → D: Induction of F-actin-bundling; when associated with D-120 and D-183. In neurons, induces the generation of long and prominent filopodia; when associated with D-120 and D-183. 1 Publication1
Mutagenesisi183T → A: Complete loss of MAPK-mediated phosphorylation; when associated with A-120 and A-148. In neurons, induces the generation of large, flat lamellipodial protrusions; when associated with A-120 and A-148. 1 Publication1
Mutagenesisi183T → D: Induction of F-actin-bundling; when associated with D-120 and D-148. In neurons, induces the generation of long and prominent filopodia; when associated with D-120 and D-148. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001571532 – 200MARCKS-related proteinAdd BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei14PhosphothreonineCombined sources1
Modified residuei22PhosphoserineCombined sources1
Modified residuei36PhosphoserineBy similarity1
Modified residuei48PhosphoserineCombined sources1
Modified residuei71PhosphoserineCombined sources1
Modified residuei85PhosphothreonineCombined sources1
Modified residuei93Phosphoserine; by PKCBy similarity1
Modified residuei101Phosphoserine; by PKCBy similarity1
Modified residuei104Phosphoserine; by PKCBy similarity1
Modified residuei119PhosphoserineCombined sources1
Modified residuei120Phosphoserine; by MAPK8Combined sources1 Publication1
Modified residuei132PhosphoserineCombined sources1
Modified residuei135PhosphoserineCombined sources1
Modified residuei148Phosphothreonine; by MAPK8Combined sources1 Publication1
Modified residuei151PhosphoserineBy similarity1
Modified residuei162PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei170PhosphothreonineCombined sources1
Modified residuei183Phosphothreonine; by MAPK8Combined sources1 Publication1
Modified residuei192PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated (PubMed:1618855, PubMed:22751924). Phosphorylation at Ser-120 and Thr-183 is non-redundantly catalyzed by MAPK8 in vivo (PubMed:22751924). Phosphorylation at Thr-148 is preferentially catalyzed by MAPK8 in vivo, but this modification can also be catalyzed by other kinases in the absence of MAPK8 (PubMed:22751924). May be phosphorylated by protein kinase C, which disrupts the interaction with calmodulin (PubMed:1618855).2 Publications

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP28667
MaxQBiP28667
PaxDbiP28667
PeptideAtlasiP28667
PRIDEiP28667

PTM databases

iPTMnetiP28667
PhosphoSitePlusiP28667
SwissPalmiP28667

Miscellaneous databases

PMAP-CutDBiP28667

Expressioni

Tissue specificityi

Expressed at high levels in brain cortex and hippocampus, including dentate gyrus, anterior olfactory nucleus, primary olfactory cortex, entorhinal cortex, medial preoptic area and dorsomedial hypothalamic nucleus (at protein level) (PubMed:1864362, PubMed:8406449, PubMed:9598313, PubMed:22751924). Expressed in neuronal cells (at protein level) (PubMed:22751924). Detected in the retina (PubMed:9598313). Strongly expressed in testis and uterus; expressed at lower levels in cerebellum, cerebrum, adipose tissue, spleen, kidney, thyroid, liver, lung, skeletal muscle and heart (PubMed:8406449). Detected in T-cells and B-cells (PubMed:8406449).4 Publications

Developmental stagei

Expressed in the developing neural tube as early as 8.5 dpc. Remains most highly expressed in the developing brain and spinal cord during later development at least until 14.5 dpc. Also detected in the lung, adrenal gland, gut and kidney, particularly the kidney cortex. Undetectable in the liver.1 Publication

Inductioni

Up-regulated in peritoneal macrophages in response to bacterial lipopolysaccharide (LPS).1 Publication

Gene expression databases

BgeeiENSMUSG00000047945 Expressed in 319 organ(s), highest expression level in neocortex
CleanExiMM_MARCKSL1
GenevisibleiP28667 MM

Interactioni

Subunit structurei

Binds to filamentous actin (F-actin), but not to monomeric G-actin, independently of its phosphorylation status (PubMed:22751924). Interacts with calmodulin (PubMed:1618855).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201439, 1 interactor
STRINGi10090.ENSMUSP00000055637

Structurei

3D structure databases

ProteinModelPortaliP28667
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 110Effector domain involved in lipid-binding and calmodulin-binding2 PublicationsAdd BLAST24

Sequence similaritiesi

Belongs to the MARCKS family.Curated

Phylogenomic databases

eggNOGiENOG410IDYZ Eukaryota
ENOG410Z8K4 LUCA
GeneTreeiENSGT00730000111349
HOGENOMiHOG000059262
HOVERGENiHBG003515
InParanoidiP28667
KOiK13536
OMAiEIGACGE
OrthoDBiEOG091G0ULD
PhylomeDBiP28667
TreeFamiTF332815

Family and domain databases

InterProiView protein in InterPro
IPR002101 MARCKS
PANTHERiPTHR14353 PTHR14353, 1 hit
PfamiView protein in Pfam
PF02063 MARCKS, 2 hits
PRINTSiPR00963 MARCKS
PROSITEiView protein in PROSITE
PS00826 MARCKS_1, 1 hit
PS00827 MARCKS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28667-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSQSSKAPR GDVTAEEAAG ASPAKANGQE NGHVRSNGDL TPKGEGESPP
60 70 80 90 100
VNGTDEAAGA TGDAIEPAPP SQEAEAKGEV APKETPKKKK KFSFKKPFKL
110 120 130 140 150
SGLSFKRNRK EGGGDSSASS PTEEEQEQGE MSACSDEGTA QEGKAAATPE
160 170 180 190 200
SQEPQAKGAE ASAASKEGDT EEEAGPQAAE PSTPSGPESG PTPASAEQNE
Length:200
Mass (Da):20,165
Last modified:January 23, 2007 - v2
Checksum:iAA50A0E2029921AF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134C → S in AAH06757 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61399 mRNA Translation: CAA43671.1
S65597 Genomic DNA Translation: AAP13962.1
AK079390 mRNA Translation: BAC37630.1
AK083913 mRNA Translation: BAC39058.1
AK152990 mRNA Translation: BAE31636.1
AK169355 mRNA Translation: BAE41104.1
BC006757 mRNA Translation: AAH06757.1
CCDSiCCDS18695.1
PIRiS17185
RefSeqiNP_034937.1, NM_010807.4
UniGeneiMm.424974

Genome annotation databases

EnsembliENSMUST00000062356; ENSMUSP00000055637; ENSMUSG00000047945
GeneIDi17357
KEGGimmu:17357
UCSCiuc008uxf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61399 mRNA Translation: CAA43671.1
S65597 Genomic DNA Translation: AAP13962.1
AK079390 mRNA Translation: BAC37630.1
AK083913 mRNA Translation: BAC39058.1
AK152990 mRNA Translation: BAE31636.1
AK169355 mRNA Translation: BAE41104.1
BC006757 mRNA Translation: AAH06757.1
CCDSiCCDS18695.1
PIRiS17185
RefSeqiNP_034937.1, NM_010807.4
UniGeneiMm.424974

3D structure databases

ProteinModelPortaliP28667
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201439, 1 interactor
STRINGi10090.ENSMUSP00000055637

PTM databases

iPTMnetiP28667
PhosphoSitePlusiP28667
SwissPalmiP28667

Proteomic databases

EPDiP28667
MaxQBiP28667
PaxDbiP28667
PeptideAtlasiP28667
PRIDEiP28667

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062356; ENSMUSP00000055637; ENSMUSG00000047945
GeneIDi17357
KEGGimmu:17357
UCSCiuc008uxf.2 mouse

Organism-specific databases

CTDi65108
MGIiMGI:97143 Marcksl1

Phylogenomic databases

eggNOGiENOG410IDYZ Eukaryota
ENOG410Z8K4 LUCA
GeneTreeiENSGT00730000111349
HOGENOMiHOG000059262
HOVERGENiHBG003515
InParanoidiP28667
KOiK13536
OMAiEIGACGE
OrthoDBiEOG091G0ULD
PhylomeDBiP28667
TreeFamiTF332815

Miscellaneous databases

PMAP-CutDBiP28667
PROiPR:P28667
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047945 Expressed in 319 organ(s), highest expression level in neocortex
CleanExiMM_MARCKSL1
GenevisibleiP28667 MM

Family and domain databases

InterProiView protein in InterPro
IPR002101 MARCKS
PANTHERiPTHR14353 PTHR14353, 1 hit
PfamiView protein in Pfam
PF02063 MARCKS, 2 hits
PRINTSiPR00963 MARCKS
PROSITEiView protein in PROSITE
PS00826 MARCKS_1, 1 hit
PS00827 MARCKS_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMRP_MOUSE
AccessioniPrimary (citable) accession number: P28667
Secondary accession number(s): Q3TEZ4, Q91W07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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