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Protein

Adenosine receptor A3

Gene

Adora3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase (PubMed:1323836). May play a role during reproduction (PubMed:1647979).1 Publication1 Publication

GO - Molecular functioni

  • G-protein coupled adenosine receptor activity Source: RGD

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: RGD
  • regulation of norepinephrine secretion Source: RGD

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-417973 Adenosine P1 receptors
R-RNO-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine receptor A3
Alternative name(s):
TGPCR1
Gene namesi
Name:Adora3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2051 Adora3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16ExtracellularBy similarityAdd BLAST16
Transmembranei17 – 39Helical; Name=1By similarityAdd BLAST23
Topological domaini40 – 50CytoplasmicBy similarityAdd BLAST11
Transmembranei51 – 74Helical; Name=2By similarityAdd BLAST24
Topological domaini75 – 86ExtracellularBy similarityAdd BLAST12
Transmembranei87 – 108Helical; Name=3By similarityAdd BLAST22
Topological domaini109 – 128CytoplasmicBy similarityAdd BLAST20
Transmembranei129 – 150Helical; Name=4By similarityAdd BLAST22
Topological domaini151 – 179ExtracellularBy similarityAdd BLAST29
Transmembranei180 – 200Helical; Name=5By similarityAdd BLAST21
Topological domaini201 – 233CytoplasmicBy similarityAdd BLAST33
Transmembranei234 – 257Helical; Name=6By similarityAdd BLAST24
Topological domaini258 – 263ExtracellularBy similarity6
Transmembranei264 – 286Helical; Name=7By similarityAdd BLAST23
Topological domaini287 – 320CytoplasmicBy similarityAdd BLAST34

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi302 – 305CRLC → ARLA: Promotes agonist-independent phosphorylation. 1 Publication4
Mutagenesisi307T → A: Small decrease in agonist-stimulated phosphorylation. 1 Publication1
Mutagenesisi318T → A or E: Strong decrease in agonist-stimulated phosphorylation. 1 Publication1
Mutagenesisi319T → A: 50% decrease in agonist-stimulated phosphorylation. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3360
GuidetoPHARMACOLOGYi21

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690131 – 320Adenosine receptor A3Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi4N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi5N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi85 ↔ 168PROSITE-ProRule annotation
Lipidationi305S-palmitoyl cysteineSequence analysis1
Modified residuei307Phosphothreonine1 Publication1
Modified residuei318Phosphothreonine1 Publication1
Modified residuei319Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylation on Thr-318 and Thr-319 may be crucial for rapid desensitization. Phosphorylation on Thr-318 may be necessary for phosphorylation on Thr-319 to occur.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP28647

PTM databases

iPTMnetiP28647
PhosphoSitePlusiP28647

Expressioni

Tissue specificityi

Testis, particularly in spermatocytes and spermatids but not in spermatogonia. Low levels in the brain.1 Publication

Developmental stagei

Expressed during spermiogenesis.1 Publication

Gene expression databases

BgeeiENSRNOG00000015788 Expressed in 9 organ(s), highest expression level in testis
GenevisibleiP28647 RN

Interactioni

Protein-protein interaction databases

DIPiDIP-217N
STRINGi10116.ENSRNOP00000046340

Chemistry databases

BindingDBiP28647

Structurei

3D structure databases

ProteinModelPortaliP28647
SMRiP28647
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00550000074524
HOGENOMiHOG000015770
HOVERGENiHBG106962
InParanoidiP28647
KOiK04268
OMAiMRMDYMV
OrthoDBiEOG091G0I3U
PhylomeDBiP28647
TreeFamiTF325296

Family and domain databases

InterProiView protein in InterPro
IPR000466 Adeno_A3_rcpt
IPR001634 Adenosn_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00555 ADENOSINEA3R
PR00424 ADENOSINER
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform A (identifier: P28647-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKANNTTTSA LWLQITYITM EAAIGLCAVV GNMLVIWVVK LNRTLRTTTF
60 70 80 90 100
YFIVSLALAD IAVGVLVIPL AIAVSLEVQM HFYACLFMSC VLLVFTHASI
110 120 130 140 150
MSLLAIAVDR YLRVKLTVRY RTVTTQRRIW LFLGLCWLVS FLVGLTPMFG
160 170 180 190 200
WNRKVTLELS QNSSTLSCHF RSVVGLDYMV FFSFITWILI PLVVMCIIYL
210 220 230 240 250
DIFYIIRNKL SQNLTGFRET RAFYGREFKT AKSLFLVLFL FALCWLPLSI
260 270 280 290 300
INFVSYFNVK IPEIAMCLGI LLSHANSMMN PIVYACKIKK FKETYFVILR
310 320
ACRLCQTSDS LDSNLEQTTE
Length:320
Mass (Da):36,629
Last modified:November 1, 1997 - v3
Checksum:i59DBA40C594ACDAB
GO
Isoform B (identifier: P28647-2) [UniParc]FASTAAdd to basket
Also known as: A3i

The sequence of this isoform differs from the canonical sequence as follows:
     119-119: R → RLSFLVVNLPFSSPHSNR

Show »
Length:337
Mass (Da):38,525
Checksum:i80350BBDE3302AA6
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JZ55A0A0G2JZ55_RAT
Adenosine receptor A3
Adora3 LOC100363178, LOC100911796, Tmigd3
319Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18I → V in AAA40680 (PubMed:1323836).Curated1
Sequence conflicti68I → V in CAA63702 (PubMed:8612733).Curated1
Sequence conflicti74 – 83VSLEVQMHFY → SAWRSRCTSM in CAA41937 (PubMed:1647979).Curated10
Sequence conflicti132F → L in CAA63702 (PubMed:8612733).Curated1
Sequence conflicti172S → F in AAA40680 (PubMed:1323836).Curated1
Sequence conflicti288I → N (PubMed:1323836).Curated1
Sequence conflicti291 – 295FKETY → VQRNH (PubMed:1323836).Curated5

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001859119R → RLSFLVVNLPFSSPHSNR in isoform B. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59249 mRNA Translation: CAA41937.1
M94152 mRNA Translation: AAA40680.1
X93219 mRNA Translation: CAA63702.1
PIRiA46152
S17177
S68678
RefSeqiNP_001289679.1, NM_001302750.1 [P28647-2]
NP_001289680.1, NM_001302751.1 [P28647-1]
NP_001289684.1, NM_001302755.1 [P28647-2]
NP_037028.2, NM_012896.3 [P28647-1]
UniGeneiRn.10457
Rn.188812

Genome annotation databases

EnsembliENSRNOT00000021236; ENSRNOP00000021236; ENSRNOG00000015788 [P28647-1]
ENSRNOT00000050538; ENSRNOP00000046340; ENSRNOG00000050743 [P28647-1]
GeneIDi100911796
25370
KEGGirno:100911796
rno:25370
UCSCiRGD:2051 rat [P28647-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59249 mRNA Translation: CAA41937.1
M94152 mRNA Translation: AAA40680.1
X93219 mRNA Translation: CAA63702.1
PIRiA46152
S17177
S68678
RefSeqiNP_001289679.1, NM_001302750.1 [P28647-2]
NP_001289680.1, NM_001302751.1 [P28647-1]
NP_001289684.1, NM_001302755.1 [P28647-2]
NP_037028.2, NM_012896.3 [P28647-1]
UniGeneiRn.10457
Rn.188812

3D structure databases

ProteinModelPortaliP28647
SMRiP28647
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-217N
STRINGi10116.ENSRNOP00000046340

Chemistry databases

BindingDBiP28647
ChEMBLiCHEMBL3360
GuidetoPHARMACOLOGYi21

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP28647
PhosphoSitePlusiP28647

Proteomic databases

PaxDbiP28647

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021236; ENSRNOP00000021236; ENSRNOG00000015788 [P28647-1]
ENSRNOT00000050538; ENSRNOP00000046340; ENSRNOG00000050743 [P28647-1]
GeneIDi100911796
25370
KEGGirno:100911796
rno:25370
UCSCiRGD:2051 rat [P28647-1]

Organism-specific databases

CTDi140
RGDi2051 Adora3

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00550000074524
HOGENOMiHOG000015770
HOVERGENiHBG106962
InParanoidiP28647
KOiK04268
OMAiMRMDYMV
OrthoDBiEOG091G0I3U
PhylomeDBiP28647
TreeFamiTF325296

Enzyme and pathway databases

ReactomeiR-RNO-417973 Adenosine P1 receptors
R-RNO-418594 G alpha (i) signalling events

Miscellaneous databases

PROiPR:P28647

Gene expression databases

BgeeiENSRNOG00000015788 Expressed in 9 organ(s), highest expression level in testis
GenevisibleiP28647 RN

Family and domain databases

InterProiView protein in InterPro
IPR000466 Adeno_A3_rcpt
IPR001634 Adenosn_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00555 ADENOSINEA3R
PR00424 ADENOSINER
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAA3R_RAT
AccessioniPrimary (citable) accession number: P28647
Secondary accession number(s): Q63792
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 1, 1997
Last modified: September 12, 2018
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
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