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Entry version 129 (31 Jul 2019)
Sequence version 2 (01 Oct 1994)
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Protein

tRNA (adenine(37)-N6)-methyltransferase

Gene

trmO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m6t6A37) of the tRNA anticodon loop of tRNA(Thr)(GGU) that read codons starting with adenosine (PubMed:9537379, PubMed:25063302). The methyl group of m6t6A37 appears to slightly improve the efficiency of the tRNA decoding ability (PubMed:9537379, PubMed:25063302). Binds to tRNA (PubMed:25063302).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei92S-adenosyl-L-methionineBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • tRNA methylation Source: EcoCyc

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG11503-MONOMER
ECOL316407:JW0191-MONOMER
MetaCyc:EG11503-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
tRNA (adenine(37)-N6)-methyltransferaseCurated (EC:2.1.1.-1 Publication)
Alternative name(s):
tRNA (m6t6A37) methyltransferase1 Publication
tRNA methyltransferase O1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:trmO1 Publication
Synonyms:tsaA1 Publication, yaeB
Ordered Locus Names:b0195, JW0191
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG11503 tsaA

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi92R → A: Inhibits tRNA m(6)t(6)A37 formation. 1 Publication1
Mutagenesisi136K → A: Inhibits tRNA m(6)t(6)A37 formation. 1 Publication1
Mutagenesisi194D → A: Inhibits tRNA m(6)t(6)A37 formation; when associated with A-196. 1 Publication1
Mutagenesisi196R → A: Inhibits tRNA m(6)t(6)A37 formation; when associated with A-194. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001556171 – 235tRNA (adenine(37)-N6)-methyltransferaseAdd BLAST235

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P28634

PRoteomics IDEntifications database

More...
PRIDEi
P28634

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

By similarity

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4259756, 15 interactors

Database of interacting proteins

More...
DIPi
DIP-11195N

Protein interaction database and analysis system

More...
IntActi
P28634, 6 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b0195

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P28634

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini6 – 147TsaA-likePROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni23 – 25S-adenosyl-L-methionine bindingBy similarity3
Regioni64 – 65S-adenosyl-L-methionine bindingBy similarity2
Regioni127 – 130S-adenosyl-L-methionine bindingBy similarity4

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4108R96 Bacteria
COG1720 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000225518

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P28634

KEGG Orthology (KO)

More...
KOi
K22900

Database for complete collections of gene phylogenies

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PhylomeDBi
P28634

Family and domain databases

Conserved Domains Database

More...
CDDi
cd09281 UPF0066, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.40.30.70, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR023370 TrmO-like_N
IPR041369 TrmO_C
IPR023368 UPF0066_cons_site
IPR040372 YaeB-like
IPR036413 YaeB-like_sf
IPR036414 YaeB_N_sf

The PANTHER Classification System

More...
PANTHERi
PTHR12818 PTHR12818, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01980 TrmO, 1 hit
PF18389 TrmO_C, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF118196 SSF118196, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00104 tRNA_TsaA, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS01318 TSAA_1, 1 hit
PS51668 TSAA_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P28634-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSFQFEQIG VIRSPYKEKF AVPRQPGLVK SANGELHLIA PYNQADAVRG
60 70 80 90 100
LEAFSHLWIL FVFHQTMEGG WRPTVRPPRL GGNARMGVFA TRSTFRPNPI
110 120 130 140 150
GMSLVELKEV VCHKDSVILK LGSLDLVDGT PVVDIKPYLP FAESLPDASA
160 170 180 190 200
SYAQSAPAAE MAVSFTAEVE KQLLTLEKRY PQLTLFIREV LAQDPRPAYR
210 220 230
KGEETGKTYA VWLHDFNVRW RVTDAGFEVF ALEPR
Length:235
Mass (Da):26,362
Last modified:October 1, 1994 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB19F8D50DECCB572
GO

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA24509 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D15061 Genomic DNA Translation: BAA03655.1
L04474 Genomic DNA Translation: AAA24509.1 Frameshift.
U70214 Genomic DNA Translation: AAB08623.1
U00096 Genomic DNA Translation: AAC73306.1
AP009048 Genomic DNA Translation: BAA77871.1

Protein sequence database of the Protein Information Resource

More...
PIRi
C47040
C64744

NCBI Reference Sequences

More...
RefSeqi
NP_414737.1, NC_000913.3
WP_000094011.1, NZ_STEB01000032.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC73306; AAC73306; b0195
BAA77871; BAA77871; BAA77871

Database of genes from NCBI RefSeq genomes

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GeneIDi
949112

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW0191
eco:b0195

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.2083

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA Translation: BAA03655.1
L04474 Genomic DNA Translation: AAA24509.1 Frameshift.
U70214 Genomic DNA Translation: AAB08623.1
U00096 Genomic DNA Translation: AAC73306.1
AP009048 Genomic DNA Translation: BAA77871.1
PIRiC47040
C64744
RefSeqiNP_414737.1, NC_000913.3
WP_000094011.1, NZ_STEB01000032.1

3D structure databases

SMRiP28634
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4259756, 15 interactors
DIPiDIP-11195N
IntActiP28634, 6 interactors
STRINGi511145.b0195

Proteomic databases

PaxDbiP28634
PRIDEiP28634

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73306; AAC73306; b0195
BAA77871; BAA77871; BAA77871
GeneIDi949112
KEGGiecj:JW0191
eco:b0195
PATRICifig|1411691.4.peg.2083

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB1466
EcoGeneiEG11503 tsaA

Phylogenomic databases

eggNOGiENOG4108R96 Bacteria
COG1720 LUCA
HOGENOMiHOG000225518
InParanoidiP28634
KOiK22900
PhylomeDBiP28634

Enzyme and pathway databases

BioCyciEcoCyc:EG11503-MONOMER
ECOL316407:JW0191-MONOMER
MetaCyc:EG11503-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P28634

Family and domain databases

CDDicd09281 UPF0066, 1 hit
Gene3Di2.40.30.70, 1 hit
InterProiView protein in InterPro
IPR023370 TrmO-like_N
IPR041369 TrmO_C
IPR023368 UPF0066_cons_site
IPR040372 YaeB-like
IPR036413 YaeB-like_sf
IPR036414 YaeB_N_sf
PANTHERiPTHR12818 PTHR12818, 1 hit
PfamiView protein in Pfam
PF01980 TrmO, 1 hit
PF18389 TrmO_C, 1 hit
SUPFAMiSSF118196 SSF118196, 1 hit
TIGRFAMsiTIGR00104 tRNA_TsaA, 1 hit
PROSITEiView protein in PROSITE
PS01318 TSAA_1, 1 hit
PS51668 TSAA_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTRMO_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P28634
Secondary accession number(s): Q47676
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1994
Last modified: July 31, 2019
This is version 129 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
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