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Protein

DNA polymerase III subunit psi

Gene

holD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the beta sliding clamp loading complex, which hydrolyzes ATP to load the beta clamp onto primed DNA to form the DNA replication pre-initiation complex (PubMed:2040637). DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The exact function of the psi subunit is unknown.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • response to radiation Source: EcoCyc

Keywordsi

Molecular functionDNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG11414-MONOMER
MetaCyc:EG11414-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit psi (EC:2.7.7.7)
Gene namesi
Name:holD
Ordered Locus Names:b4372, JW4334
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11414 holD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001055201 – 137DNA polymerase III subunit psiAdd BLAST137

Proteomic databases

PaxDbiP28632
PRIDEiP28632

Interactioni

Subunit structurei

The DNA polymerase III holoenzyme complex contains at least 10 different subunits organized into 3 functionally essential subassemblies: the Pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The Pol III core (subunits alpha, epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities (PubMed:2040637). The polymerase is tethered to the template via the dimeric beta sliding clamp processivity factor. The clamp-loading complex (also called gamma complex) assembles the beta sliding clamp onto the primed template and plays a central role in the organization and communication at the replication fork. The clamp-loading complex contains delta, delta', psi and chi, and 3 copies of either or both of two different DnaX proteins, gamma and tau. The DNA replisome complex has a single clamp loader (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase (PubMed:20413500, PubMed:22157955). The clamp loader hydrolyzes ATP to assemble the beta processivity factor onto the primed template (PubMed:2040637) and plays a central role in the organization and communication at the replication fork.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4262785, 117 interactors
ComplexPortaliCPX-1926 DNA polymerase III clamp loader complex
CPX-1964 DNA polymerase III clamp loader chi-psi subcomplex
DIPiDIP-9934N
IntActiP28632, 23 interactors
MINTiP28632
STRINGi316385.ECDH10B_4529

Structurei

Secondary structure

1137
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 12Combined sources9
Beta strandi16 – 20Combined sources5
Helixi22 – 24Combined sources3
Beta strandi39 – 42Combined sources4
Helixi52 – 61Combined sources10
Helixi65 – 67Combined sources3
Beta strandi68 – 71Combined sources4
Helixi73 – 76Combined sources4
Beta strandi87 – 91Combined sources5
Beta strandi97 – 100Combined sources4
Beta strandi102 – 105Combined sources4
Helixi108 – 113Combined sources6
Helixi115 – 127Combined sources13
Helixi129 – 132Combined sources4

3D structure databases

ProteinModelPortaliP28632
SMRiP28632
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28632

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108WPU Bacteria
COG3050 LUCA
HOGENOMiHOG000218328
KOiK02344
OMAiQICAHEH

Family and domain databases

Gene3Di3.40.50.10220, 1 hit
InterProiView protein in InterPro
IPR004615 DNA_pol_III_psi
IPR036654 DNA_pol_III_psi_sf
IPR018382 DNA_pol_III_psi_subgr
PfamiView protein in Pfam
PF03603 DNA_III_psi, 1 hit
PIRSFiPIRSF029225 DNA_pol_III_psi, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD032475 DNA_pol_III_psi, 1 hit
SUPFAMiSSF102220 SSF102220, 1 hit
TIGRFAMsiTIGR00664 DNA_III_psi, 1 hit

Sequencei

Sequence statusi: Complete.

P28632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRRDWQLQ QLGITQWSLR RPGALQGEIA IAIPAHVRLV MVANDLPALT
60 70 80 90 100
DPLVSDVLRA LTVSPDQVLQ LTPEKIAMLP QGSHCNSWRL GTDEPLSLEG
110 120 130
AQVASPALTD LRANPTARAA LWQQICTYEH DFFPRND
Length:137
Mass (Da):15,174
Last modified:December 1, 1992 - v1
Checksum:i5B7356201E7E27FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04575 Genomic DNA Translation: AAA24435.1
L05387 Unassigned DNA Translation: AAA03076.1
X06117 Genomic DNA No translation available.
U14003 Genomic DNA Translation: AAA97268.1
U00096 Genomic DNA Translation: AAC77325.1
AP009048 Genomic DNA Translation: BAE78360.1
PIRiA48647
RefSeqiNP_418789.1, NC_000913.3
WP_000204012.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77325; AAC77325; b4372
BAE78360; BAE78360; BAE78360
GeneIDi948890
KEGGiecj:JW4334
eco:b4372
PATRICifig|1411691.4.peg.2316

Similar proteinsi

Entry informationi

Entry nameiHOLD_ECOLI
AccessioniPrimary (citable) accession number: P28632
Secondary accession number(s): Q2M5U6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 20, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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