UniProtKB - P28630 (HOLA_ECOLI)
DNA polymerase III subunit delta
holA
Functioni
Catalytic activityi
- EC:2.7.7.7
GO - Molecular functioni
- DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- DNA-dependent DNA replication Source: EcoCyc
Keywordsi
Molecular function | DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11412-MONOMER MetaCyc:EG11412-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:holA Ordered Locus Names:b0640, JW0635 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105505 | 1 – 343 | DNA polymerase III subunit deltaAdd BLAST | 343 |
Proteomic databases
jPOSTi | P28630 |
PaxDbi | P28630 |
PRIDEi | P28630 |
Interactioni
Subunit structurei
The DNA polymerase III holoenzyme complex contains at least 10 different subunits organized into 3 functionally essential subassemblies: the Pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The Pol III core (subunits alpha, epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities (PubMed:2040637). The polymerase is tethered to the template via the dimeric beta sliding clamp processivity factor. The clamp-loading complex (also called gamma complex) assembles the beta sliding clamp onto the primed template and plays a central role in the organization and communication at the replication fork. The clamp-loading complex contains delta, delta', psi and chi, and 3 copies of either or both of two different DnaX proteins, gamma and tau. The DNA replisome complex has a single clamp loader (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase (PubMed:20413500, PubMed:22157955). The clamp loader hydrolyzes ATP to assemble the beta processivity factor onto the primed template (PubMed:2040637, PubMed:9927437) and plays a central role in the organization and communication at the replication fork; the minimal complex to load the beta sliding clamp on DNA is delta, delta', gamma (PubMed:9927437).
5 PublicationsBinary interactionsi
Hide detailsP28630
Protein-protein interaction databases
BioGRIDi | 4259908, 201 interactors 851889, 23 interactors |
ComplexPortali | CPX-1926, DNA polymerase III clamp loader complex |
DIPi | DIP-9931N |
IntActi | P28630, 49 interactors |
STRINGi | 511145.b0640 |
Structurei
Secondary structure
3D structure databases
SMRi | P28630 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28630 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 140 | Domain 11 PublicationAdd BLAST | 140 | |
Regioni | 141 – 210 | Domain 21 PublicationAdd BLAST | 70 | |
Regioni | 211 – 343 | Domain 31 PublicationAdd BLAST | 133 |
Domaini
Phylogenomic databases
eggNOGi | COG1466, Bacteria |
HOGENOMi | CLU_044694_0_2_6 |
InParanoidi | P28630 |
PhylomeDBi | P28630 |
Family and domain databases
InterProi | View protein in InterPro IPR008921, DNA_pol3_clamp-load_cplx_C IPR032780, DNA_pol3_delt_C IPR010372, DNA_pol3_delta_N IPR005790, DNA_polIII_delta IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF14840, DNA_pol3_delt_C, 1 hit PF06144, DNA_pol3_delta, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01128, holA, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MIRLYPEQLR AQLNEGLRAA YLLLGNDPLL LQESQDAVRQ VAAAQGFEEH
60 70 80 90 100
HTFSIDPNTD WNAIFSLCQA MSLFASRQTL LLLLPENGPN AAINEQLLTL
110 120 130 140 150
TGLLHDDLLL IVRGNKLSKA QENAAWFTAL ANRSVQVTCQ TPEQAQLPRW
160 170 180 190 200
VAARAKQLNL ELDDAANQVL CYCYEGNLLA LAQALERLSL LWPDGKLTLP
210 220 230 240 250
RVEQAVNDAA HFTPFHWVDA LLMGKSKRAL HILQQLRLEG SEPVILLRTL
260 270 280 290 300
QRELLLLVNL KRQSAHTPLR ALFDKHRVWQ NRRGMMGEAL NRLSQTQLRQ
310 320 330 340
AVQLLTRTEL TLKQDYGQSV WAELEGLSLL LCHKPLADVF IDG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04576 Genomic DNA Translation: AAA23675.1 M94267 Genomic DNA Translation: AAB59047.1 U82598 Genomic DNA Translation: AAB40841.1 U00096 Genomic DNA Translation: AAC73741.1 AP009048 Genomic DNA Translation: BAA35287.1 M18277 Genomic DNA No translation available. |
PIRi | A45251 |
RefSeqi | NP_415173.1, NC_000913.3 WP_000620535.1, NZ_SSZK01000037.1 |
Genome annotation databases
EnsemblBacteriai | AAC73741; AAC73741; b0640 BAA35287; BAA35287; BAA35287 |
GeneIDi | 947573 |
KEGGi | ecj:JW0635 eco:b0640 |
PATRICi | fig|1411691.4.peg.1628 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04576 Genomic DNA Translation: AAA23675.1 M94267 Genomic DNA Translation: AAB59047.1 U82598 Genomic DNA Translation: AAB40841.1 U00096 Genomic DNA Translation: AAC73741.1 AP009048 Genomic DNA Translation: BAA35287.1 M18277 Genomic DNA No translation available. |
PIRi | A45251 |
RefSeqi | NP_415173.1, NC_000913.3 WP_000620535.1, NZ_SSZK01000037.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JQJ | X-ray | 2.90 | C/D | 1-343 | [»] | |
1JQL | X-ray | 2.50 | B | 1-140 | [»] | |
1JR3 | X-ray | 2.70 | D | 1-343 | [»] | |
1XXH | X-ray | 3.45 | A/F | 1-343 | [»] | |
1XXI | X-ray | 4.10 | A/F | 1-343 | [»] | |
3GLF | X-ray | 3.39 | A/F | 1-343 | [»] | |
3GLG | X-ray | 3.25 | A/F | 1-343 | [»] | |
3GLH | X-ray | 3.89 | A/F/K | 1-343 | [»] | |
3GLI | X-ray | 3.50 | A/F | 1-343 | [»] | |
SMRi | P28630 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259908, 201 interactors 851889, 23 interactors |
ComplexPortali | CPX-1926, DNA polymerase III clamp loader complex |
DIPi | DIP-9931N |
IntActi | P28630, 49 interactors |
STRINGi | 511145.b0640 |
Proteomic databases
jPOSTi | P28630 |
PaxDbi | P28630 |
PRIDEi | P28630 |
Genome annotation databases
EnsemblBacteriai | AAC73741; AAC73741; b0640 BAA35287; BAA35287; BAA35287 |
GeneIDi | 947573 |
KEGGi | ecj:JW0635 eco:b0640 |
PATRICi | fig|1411691.4.peg.1628 |
Organism-specific databases
EchoBASEi | EB1384 |
Phylogenomic databases
eggNOGi | COG1466, Bacteria |
HOGENOMi | CLU_044694_0_2_6 |
InParanoidi | P28630 |
PhylomeDBi | P28630 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11412-MONOMER MetaCyc:EG11412-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P28630 |
PROi | PR:P28630 |
Family and domain databases
InterProi | View protein in InterPro IPR008921, DNA_pol3_clamp-load_cplx_C IPR032780, DNA_pol3_delt_C IPR010372, DNA_pol3_delta_N IPR005790, DNA_polIII_delta IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF14840, DNA_pol3_delt_C, 1 hit PF06144, DNA_pol3_delta, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01128, holA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HOLA_ECOLI | |
Accessioni | P28630Primary (citable) accession number: P28630 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references