UniProtKB - P28619 (RNPH_BACSU)
Protein
Ribonuclease PH
Gene
rph
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. Plays a role in the secondary pathway of 23S rRNA 3' end maturation (PubMed:19880604).UniRule annotation1 Publication
Miscellaneous
Sulfate ions in the crystal structure may represent the inorganic phosphate substrate.1 Publication
Catalytic activityi
- EC:2.7.7.56UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 86 | Phosphate (substrate) bindingUniRule annotation | 1 |
GO - Molecular functioni
- 3'-5'-exoribonuclease activity Source: UniProtKB-UniRule
- tRNA binding Source: UniProtKB-UniRule
- tRNA nucleotidyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- rRNA 3'-end processing Source: UniProtKB
- rRNA catabolic process Source: GO_Central
- tRNA processing Source: UniProtKB-UniRule
Keywordsi
Molecular function | Nucleotidyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | rRNA processing, tRNA processing |
Enzyme and pathway databases
BioCyci | BSUB:BSU28370-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)Short name: RNase PHUniRule annotation Alternative name(s): tRNA nucleotidyltransferaseUniRule annotation |
Gene namesi | Name:rphUniRule annotation Ordered Locus Names:BSU28370 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Correct processing of the 3' end of 23S rRNA no longer occurs in the absence of mrnC.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 68 – 76 | RATNQRTIR → QATNQQTIQ: Protein crystallizes as a dimer. 1 Publication | 9 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139869 | 1 – 245 | Ribonuclease PHAdd BLAST | 245 |
Proteomic databases
jPOSTi | P28619 |
PaxDbi | P28619 |
PRIDEi | P28619 |
Interactioni
Subunit structurei
Homohexameric ring arranged as a trimer of dimers (PubMed:14767080). It has been suggested that the active form is the dimer which binds tRNA and that the hexameric form protects the substrate recognition loop (approximately residues 65-82) from proteolysis (PubMed:14767080).
UniRule annotation1 PublicationProtein-protein interaction databases
STRINGi | 224308.BSU28370 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28619 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28619 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 124 – 126 | Phosphate (substrate) bindingUniRule annotation1 Publication | 3 |
Sequence similaritiesi
Belongs to the RNase PH family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0689, Bacteria |
InParanoidi | P28619 |
OMAi | KGKGQGW |
PhylomeDBi | P28619 |
Family and domain databases
CDDi | cd11362, RNase_PH_bact, 1 hit |
Gene3Di | 3.30.230.70, 1 hit |
HAMAPi | MF_00564, RNase_PH, 1 hit |
InterProi | View protein in InterPro IPR001247, ExoRNase_PH_dom1 IPR015847, ExoRNase_PH_dom2 IPR036345, ExoRNase_PH_dom2_sf IPR027408, PNPase/RNase_PH_dom_sf IPR020568, Ribosomal_S5_D2-typ_fold IPR002381, RNase_PH_bac-type IPR018336, RNase_PH_CS |
Pfami | View protein in Pfam PF01138, RNase_PH, 1 hit PF03725, RNase_PH_C, 1 hit |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55666, SSF55666, 1 hit |
TIGRFAMsi | TIGR01966, RNasePH, 1 hit |
PROSITEi | View protein in PROSITE PS01277, RIBONUCLEASE_PH, 1 hit |
i Sequence
Sequence statusi: Complete.
P28619-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRHDGRQHDE LRPITFDLDF ISHPEGSVLI TAGNTKVICN ASVEDRVPPF
60 70 80 90 100
LRGGGKGWIT AEYSMLPRAT NQRTIRESSK GKISGRTMEI QRLIGRALRA
110 120 130 140 150
VVDLEKLGER TIWIDCDVIQ ADGGTRTASI TGAFLAMAIA IGKLIKAGTI
160 170 180 190 200
KTNPITDFLA AISVGIDKEQ GILLDLNYEE DSSAEVDMNV IMTGSGRFVE
210 220 230 240
LQGTGEEATF SREDLNGLLG LAEKGIQELI DKQKEVLGDS LPELK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M85163 Genomic DNA Translation: AAA22705.1 Z75208 Genomic DNA Translation: CAA99554.1 AL009126 Genomic DNA Translation: CAB14797.1 |
PIRi | A44914 |
RefSeqi | NP_390715.1, NC_000964.3 WP_003246039.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14797; CAB14797; BSU28370 |
GeneIDi | 51992458 937463 |
KEGGi | bsu:BSU28370 |
PATRICi | fig|224308.179.peg.3082 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M85163 Genomic DNA Translation: AAA22705.1 Z75208 Genomic DNA Translation: CAA99554.1 AL009126 Genomic DNA Translation: CAB14797.1 |
PIRi | A44914 |
RefSeqi | NP_390715.1, NC_000964.3 WP_003246039.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OYP | X-ray | 2.76 | A/B/C/D/E/F | 1-245 | [»] | |
1OYR | X-ray | 3.10 | A/B/C/D/E/F | 1-245 | [»] | |
1OYS | X-ray | 2.40 | A | 1-245 | [»] | |
SMRi | P28619 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU28370 |
Proteomic databases
jPOSTi | P28619 |
PaxDbi | P28619 |
PRIDEi | P28619 |
Genome annotation databases
EnsemblBacteriai | CAB14797; CAB14797; BSU28370 |
GeneIDi | 51992458 937463 |
KEGGi | bsu:BSU28370 |
PATRICi | fig|224308.179.peg.3082 |
Phylogenomic databases
eggNOGi | COG0689, Bacteria |
InParanoidi | P28619 |
OMAi | KGKGQGW |
PhylomeDBi | P28619 |
Enzyme and pathway databases
BioCyci | BSUB:BSU28370-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P28619 |
PROi | PR:P28619 |
Family and domain databases
CDDi | cd11362, RNase_PH_bact, 1 hit |
Gene3Di | 3.30.230.70, 1 hit |
HAMAPi | MF_00564, RNase_PH, 1 hit |
InterProi | View protein in InterPro IPR001247, ExoRNase_PH_dom1 IPR015847, ExoRNase_PH_dom2 IPR036345, ExoRNase_PH_dom2_sf IPR027408, PNPase/RNase_PH_dom_sf IPR020568, Ribosomal_S5_D2-typ_fold IPR002381, RNase_PH_bac-type IPR018336, RNase_PH_CS |
Pfami | View protein in Pfam PF01138, RNase_PH, 1 hit PF03725, RNase_PH_C, 1 hit |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55666, SSF55666, 1 hit |
TIGRFAMsi | TIGR01966, RNasePH, 1 hit |
PROSITEi | View protein in PROSITE PS01277, RIBONUCLEASE_PH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNPH_BACSU | |
Accessioni | P28619Primary (citable) accession number: P28619 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families