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UniProtKB - P28598 (CH60_BACSU)
Protein
Chaperonin GroEL
Gene
groEL
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.
UniRule annotationCatalytic activityi
- ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.UniRule annotation EC:5.6.1.7
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 413 | ATP; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 492 | ATPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 29 – 32 | ATPUniRule annotation | 4 | |
Nucleotide bindingi | 86 – 90 | ATPUniRule annotation | 5 | |
Nucleotide bindingi | 476 – 478 | ATPUniRule annotation | 3 |
GO - Molecular functioni
- ATP binding Source: GO_Central
- ATP hydrolysis activity Source: InterPro
- isomerase activity Source: UniProtKB-KW
- unfolded protein binding Source: GO_Central
GO - Biological processi
- chaperone cofactor-dependent protein refolding Source: GO_Central
- protein folding Source: GO_Central
- protein refolding Source: GO_Central
- response to heat Source: GO_Central
Keywordsi
Molecular function | Chaperone, Isomerase |
Biological process | Stress response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU06030-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Chaperonin GroELUniRule annotation (EC:5.6.1.7UniRule annotation)Alternative name(s): 60 kDa chaperoninUniRule annotation Chaperonin-60UniRule annotation Short name: Cpn60UniRule annotation |
Gene namesi | Name:groELUniRule annotation Synonyms:groLUniRule annotation, mopA Ordered Locus Names:BSU06030 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- GroEL-GroES complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000063279 | 2 – 544 | Chaperonin GroELAdd BLAST | 543 |
Proteomic databases
jPOSTi | P28598 |
PaxDbi | P28598 |
PRIDEi | P28598 |
Interactioni
Subunit structurei
Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back.
Interacts with the co-chaperonin GroES.
UniRule annotationGO - Molecular functioni
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
DIPi | DIP-58540N |
IntActi | P28598, 1 interactor |
STRINGi | 224308.BSU06030 |
Family & Domainsi
Sequence similaritiesi
Belongs to the chaperonin (HSP60) family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0459, Bacteria |
InParanoidi | P28598 |
OMAi | TDTDKME |
PhylomeDBi | P28598 |
Family and domain databases
CDDi | cd03344, GroEL, 1 hit |
Gene3Di | 1.10.560.10, 1 hit 3.30.260.10, 1 hit 3.50.7.10, 1 hit |
HAMAPi | MF_00600, CH60, 1 hit |
InterProi | View protein in InterPro IPR018370, Chaperonin_Cpn60_CS IPR001844, Chaprnin_Cpn60 IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00298, CHAPERONIN60 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
TIGRFAMsi | TIGR02348, GroEL, 1 hit |
PROSITEi | View protein in PROSITE PS00296, CHAPERONINS_CPN60, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P28598-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKEIKFSEE ARRAMLRGVD ALADAVKVTL GPKGRNVVLE KKFGSPLITN
60 70 80 90 100
DGVTIAKEIE LEDAFENMGA KLVAEVASKT NDVAGDGTTT ATVLAQAMIR
110 120 130 140 150
EGLKNVTAGA NPVGVRKGME QAVAVAIENL KEISKPIEGK ESIAQVAAIS
160 170 180 190 200
AADEEVGSLI AEAMERVGND GVITIEESKG FTTELEVVEG MQFDRGYASP
210 220 230 240 250
YMVTDSDKME AVLDNPYILI TDKKITNIQE ILPVLEQVVQ QGKPLLLIAE
260 270 280 290 300
DVEGEALATL VVNKLRGTFN AVAVKAPGFG DRRKAMLEDI AVLTGGEVIT
310 320 330 340 350
EDLGLDLKST QIAQLGRASK VVVTKENTTI VEGAGETDKI SARVTQIRAQ
360 370 380 390 400
VEETTSEFDR EKLQERLAKL AGGVAVIKVG AATETELKER KLRIEDALNS
410 420 430 440 450
TRAAVEEGIV SGGGTALVNV YNKVAAVEAE GDAQTGINIV LRALEEPIRQ
460 470 480 490 500
IAHNAGLEGS VIVERLKNEE IGVGFNAATG EWVNMIEKGI VDPTKVTRSA
510 520 530 540
LQNAASVAAM FLTTEAVVAD KPEENGGGAG MPDMGGMGGM GGMM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | M → N in BAA22519 (PubMed:1369494).Curated | 1 | |
Sequence conflicti | 125 | V → L in BAA22519 (PubMed:1369494).Curated | 1 | |
Sequence conflicti | 202 | M → L in BAA22519 (PubMed:1369494).Curated | 1 | |
Sequence conflicti | 375 | A → R in BAA22519 (PubMed:1369494).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84965 Genomic DNA Translation: AAA22531.1 M81132 Genomic DNA Translation: AAA22503.1 D10972 Genomic DNA Translation: BAA22519.1 AB007637 Genomic DNA Translation: BAA22747.1 AL009126 Genomic DNA Translation: CAB12422.1 D88802 Genomic DNA Translation: BAA19727.1 |
PIRi | B41884 |
RefSeqi | NP_388484.1, NC_000964.3 WP_003243151.1, NZ_JNCM01000032.1 |
Genome annotation databases
EnsemblBacteriai | CAB12422; CAB12422; BSU_06030 |
GeneIDi | 938045 |
KEGGi | bsu:BSU06030 |
PATRICi | fig|224308.179.peg.648 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84965 Genomic DNA Translation: AAA22531.1 M81132 Genomic DNA Translation: AAA22503.1 D10972 Genomic DNA Translation: BAA22519.1 AB007637 Genomic DNA Translation: BAA22747.1 AL009126 Genomic DNA Translation: CAB12422.1 D88802 Genomic DNA Translation: BAA19727.1 |
PIRi | B41884 |
RefSeqi | NP_388484.1, NC_000964.3 WP_003243151.1, NZ_JNCM01000032.1 |
3D structure databases
SMRi | P28598 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-58540N |
IntActi | P28598, 1 interactor |
STRINGi | 224308.BSU06030 |
Proteomic databases
jPOSTi | P28598 |
PaxDbi | P28598 |
PRIDEi | P28598 |
Genome annotation databases
EnsemblBacteriai | CAB12422; CAB12422; BSU_06030 |
GeneIDi | 938045 |
KEGGi | bsu:BSU06030 |
PATRICi | fig|224308.179.peg.648 |
Phylogenomic databases
eggNOGi | COG0459, Bacteria |
InParanoidi | P28598 |
OMAi | TDTDKME |
PhylomeDBi | P28598 |
Enzyme and pathway databases
BioCyci | BSUB:BSU06030-MONOMER |
Family and domain databases
CDDi | cd03344, GroEL, 1 hit |
Gene3Di | 1.10.560.10, 1 hit 3.30.260.10, 1 hit 3.50.7.10, 1 hit |
HAMAPi | MF_00600, CH60, 1 hit |
InterProi | View protein in InterPro IPR018370, Chaperonin_Cpn60_CS IPR001844, Chaprnin_Cpn60 IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00298, CHAPERONIN60 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
TIGRFAMsi | TIGR02348, GroEL, 1 hit |
PROSITEi | View protein in PROSITE PS00296, CHAPERONINS_CPN60, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CH60_BACSU | |
Accessioni | P28598Primary (citable) accession number: P28598 Secondary accession number(s): O05526 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families