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Protein

Platelet-derived growth factor subunit A

Gene

Pdgfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Growth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-RNO-114608 Platelet degranulation
R-RNO-1257604 PIP3 activates AKT signaling
R-RNO-186763 Downstream signal transduction
R-RNO-186797 Signaling by PDGF
R-RNO-3000171 Non-integrin membrane-ECM interactions
R-RNO-5673001 RAF/MAP kinase cascade
R-RNO-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-derived growth factor subunit A
Short name:
PDGF subunit A
Alternative name(s):
PDGF-1
Platelet-derived growth factor A chain
Platelet-derived growth factor alpha polypeptide
Gene namesi
Name:Pdgfa
Synonyms:Rpa1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi3282 Pdgfa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
PropeptideiPRO_000002336221 – 85Removed in mature formAdd BLAST65
ChainiPRO_000002336386 – 204Platelet-derived growth factor subunit AAdd BLAST119

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi96 ↔ 140By similarity
Disulfide bondi123InterchainBy similarity
Disulfide bondi129 ↔ 177By similarity
Disulfide bondi132InterchainBy similarity
Disulfide bondi133 ↔ 179By similarity
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP28576
PRIDEiP28576

Expressioni

Developmental stagei

In kidney epithelial tissues, the shorter form predominates in young (1 day old) rats while the longer form becomes more prevalant during aging.

Gene expression databases

BgeeiENSRNOG00000001312
ExpressionAtlasiP28576 baseline and differential
GenevisibleiP28576 RN

Interactioni

Subunit structurei

Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFB; antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001775

Chemistry databases

BindingDBiP28576

Structurei

3D structure databases

ProteinModelPortaliP28576
SMRiP28576
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni158 – 162Receptor binding siteSequence analysis5

Domaini

The long form contains a basic insert which acts as a cell retention signal.

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEXJ Eukaryota
ENOG4111GMH LUCA
GeneTreeiENSGT00510000046755
HOVERGENiHBG053546
InParanoidiP28576
KOiK04359
PhylomeDBiP28576

Family and domain databases

CDDicd00135 PDGF, 1 hit
Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR023581 PD_growth_factor_CS
IPR000072 PDGF/VEGF_dom
IPR006782 PDGF_N
PfamiView protein in Pfam
PF00341 PDGF, 1 hit
PF04692 PDGF_N, 1 hit
SMARTiView protein in SMART
SM00141 PDGF, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS00249 PDGF_1, 1 hit
PS50278 PDGF_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P28576-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRTWACLLLL GCGYLAHALA EEAEIPRELI ERLARSQIHS IRDLQRLLEI
60 70 80 90 100
DSVGAEDALE TNLRAHGSHT VKHVPEKRPV PIRRKRSIEE AIPAVCKTRT
110 120 130 140 150
VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV
160 170 180 190 200
KVAKVEYVRK KPKLKEVQVR LEEHLECACA TSNLNPDHRE EETGRRRESG

KKRK
Length:204
Mass (Da):23,307
Last modified:February 1, 1994 - v2
Checksum:iFA413F74E86F742C
GO
Isoform Short (identifier: P28576-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-196: GRR → DVR
     197-204: Missing.

Show »
Length:196
Mass (Da):22,338
Checksum:i204910ED38EEA1F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85 – 111KRSIE…RSQVD → REVLRKPFPQFARPGRSFTR YLGARWT in BAA00987 (PubMed:8447423).CuratedAdd BLAST27
Sequence conflicti119I → T in AAA41932 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004609194 – 196GRR → DVR in isoform Short. 2 Publications3
Alternative sequenceiVSP_004610197 – 204Missing in isoform Short. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06894 mRNA Translation: AAB59693.1
Z14120 mRNA Translation: CAA78490.1
D10106 mRNA Translation: BAA00987.1
L06238 mRNA Translation: AAA41932.1
S57864 mRNA Translation: AAB26134.2
PIRiA48851
S25096
RefSeqiNP_036933.1, NM_012801.1
UniGeneiRn.10999

Genome annotation databases

EnsembliENSRNOT00000001775; ENSRNOP00000001775; ENSRNOG00000001312 [P28576-2]
ENSRNOT00000042117; ENSRNOP00000040116; ENSRNOG00000001312 [P28576-2]
GeneIDi25266
KEGGirno:25266
UCSCiRGD:3282 rat [P28576-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPDGFA_RAT
AccessioniPrimary (citable) accession number: P28576
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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