Max

Functionⁱ

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. CpG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC:MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements (By similarity).By similarity

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
- 12970171
- 8521822
DNA-binding transcription factor activity Source: MGI
E-box binding Source: MGI
protein-containing complex binding Source: MGI
protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific Source: UniProtKB
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 8521822
RNA polymerase II regulatory region sequence-specific DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
sequence-specific DNA binding Source: MGI
transcription factor activity, RNA polymerase II core promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 8521822

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to peptide hormone stimulus Source: Ensembl
cellular response to starvation Source: Ensembl
negative regulation of gene expression Source: MGI
negative regulation of transcription by RNA polymerase II Source: MGI
neuron apoptotic process Source: Ensembl
positive regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from direct assayⁱ
- 10601024
protein-containing complex assembly Source: MGI
regulation of transcription, DNA-templated
Source: MGI
Inferred from genetic interactionⁱ
- 8521822
regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from direct assayⁱ
- 8521822
response to axon injury Source: Ensembl
response to insulin Source: Ensembl
retina development in camera-type eye Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-8953750 Transcriptional Regulation by E2F6

Reactomeⁱ

R-MMU-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein maxBy similarity Alternative name(s): Myc-associated factor XBy similarity Myc-binding novel HLH/LZ protein Protein myn
Gene namesⁱ	Name:MaxImported Synonyms:Myn
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 12

Organism-specific databases

MGIⁱ	MGI:96921 Max

Keywords - Cellular componentⁱ

Cell projection, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000127270	2 – 160	Protein maxAdd BLAST		159

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineBy similarity	1
Modified residueⁱ	2	PhosphoserineBy similarity	1
Modified residueⁱ	11	PhosphoserineBy similarity	1
Modified residueⁱ	66	N6-acetyllysineBy similarity	1
Modified residueⁱ	107	PhosphoserineBy similarity	1
Modified residueⁱ	153	N6-acetyllysineBy similarity	1
Modified residueⁱ	154	N6-acetyllysineBy similarity	1

Post-translational modificationⁱ

Phosphorylated.Curated

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	P28574
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P28574
PaxDbⁱ	P28574
PeptideAtlas More...PeptideAtlasⁱ	P28574
PRIDEⁱ	P28574

PTM databases

iPTMnetⁱ	P28574
PhosphoSitePlusⁱ	P28574

Expressionⁱ

Inductionⁱ

By serum; in 3T3 fibroblasts.

Gene expression databases

Bgeeⁱ	ENSMUSG00000059436 Expressed in 246 organ(s), highest expression level in small intestine
CleanExⁱ	MM_MAX

Interactionⁱ

Subunit structureⁱ

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MYC or MAD. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with SPAG9. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
Ccnt1	Q9QWV9	2	EBI-1183003,EBI-2655009
Cdk9	Q99J95	2	EBI-1183003,EBI-2654963
Myc	P01108	6	EBI-1183003,EBI-1183114

GO - Molecular functionⁱ

protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-105 Transcriptional repressor Mad-Max complex CPX-97 Transcriptional activator Myc-Max complex
CORUMⁱ	P28574
Database of interacting proteins More...DIPⁱ	DIP-116N
IntActⁱ	P28574, 78 interactors
Molecular INTeraction database More...MINTⁱ	P28574
STRINGⁱ	10090.ENSMUSP00000106025

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Helixⁱ	30 – 36	Combined sources		7
Helixⁱ	74 – 101	Combined sources		28

Show more details

3D structure databases

ProteinModelPortalⁱ	P28574
SMRⁱ	P28574
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P28574

EvolutionaryTraceⁱ

P28574

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	23 – 74	bHLHPROSITE-ProRule annotationAdd BLAST		52

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	81 – 102	Leucine-zipperAdd BLAST		22

Sequence similaritiesⁱ

Belongs to the MAX family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2483 Eukaryota ENOG4111V1C LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000064011
HOGENOMⁱ	HOG000293257
HOVERGENⁱ	HBG008542
InParanoidⁱ	P28574
KEGG Orthology (KO) More...KOⁱ	K04453
OMAⁱ	YTNRKGS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G1312
PhylomeDBⁱ	P28574
TreeFamⁱ	TF318841

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR037933 MAX
PANTHERⁱ	PTHR10328 PTHR10328, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: P28574-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDNDDIEVE SDEEQPRFQS AADKRAHHNA LERKRRDHIK DSFHSLRDSV 
        60         70         80         90        100
PSLQGEKASR AQILDKATEY IQYMRRKNHT HQQDIDDLKR QNALLEQQVR 
       110        120        130        140        150
ALEKARSSAQ LQTNYPSSDN SLYTNAKGGT ISAFDGGSDS SSESEPEEPQ 
       160
SRKKLRMEAS

Length:160

Mass (Da):18,245

Last modified:May 23, 2018 - v2

Checksum:ⁱ86D133137727A57A

GO

Isoform 2 (identifier: P28574-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
13-21: Missing.

« Hide

        10         20         30         40         50
MSDNDDIEVE SDADKRAHHN ALERKRRDHI KDSFHSLRDS VPSLQGEKAS 
        60         70         80         90        100
RAQILDKATE YIQYMRRKNH THQQDIDDLK RQNALLEQQV RALEKARSSA 
       110        120        130        140        150
QLQTNYPSSD NSLYTNAKGG TISAFDGGSD SSSESEPEEP QSRKKLRMEA 

S

Show »

Length:151

Mass (Da):17,172

Checksum:ⁱA6A836F5F39793EF

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A1W2P7P4	A0A1W2P7P4_MOUSE	Protein max Protein max	Max	124	Annotation score:
A0A1W2P702	A0A1W2P702_MOUSE	Protein max Protein max	Max	14	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	16	P → A in AAA39797 (PubMed:1840505).		1
Sequence conflictⁱ	79	H → D in AAA39797 (PubMed:1840505).		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_059578	13 – 21	Missing in isoform 2. Curated		9

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M63903 mRNA Translation: AAA39797.1 AK080431 mRNA Translation: BAC37914.1 AC124556 Genomic DNA No translation available. BC138671 mRNA Translation: AAI38672.1 BC138672 mRNA Translation: AAI38673.1 BC145369 mRNA Translation: AAI45370.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS36479.1 [P28574-1] CCDS49094.1 [P28574-2]
PIRⁱ	A38488
NCBI Reference Sequences More...RefSeqⁱ	NP_001139648.1, NM_001146176.1 [P28574-2] NP_032584.2, NM_008558.2 [P28574-1]
UniGeneⁱ	Mm.268548 Mm.491131

Genome annotation databases

Ensemblⁱ	ENSMUST00000082136; ENSMUSP00000080778; ENSMUSG00000059436 [P28574-2] ENSMUST00000110395; ENSMUSP00000106025; ENSMUSG00000059436 [P28574-1]
GeneIDⁱ	17187
KEGGⁱ	mmu:17187
UCSC genome browser More...UCSCⁱ	uc007nyw.2 mouse uc007nyx.2 mouse

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P28574	protein max isoform X4		Florida manatee		160	UniRef100_P28574
protein max isoform X1		MESAU		160
Protein L-Myc-1-like protein		CRIGR		160
P28574-2	Protein L-Myc-1-like protein		CRIGR		151	UniRef100_A0A061I643
protein max isoform X6		Florida manatee		151
protein max isoform X2		MESAU		151

Protein	Similar proteins		Species	Score	Length	Source
P28574	Protein max	)	HUMAN		160	UniRef90_P61244
Protein max		FELCA		160
Protein max		RAT		160
MAX isoform 7		PANTR		160
Protein max isoform a		CALJA		160
+95
P28574-2	Isoform Short of Protein max		RAT		151	UniRef90_P52164-2
MYC associated factor X		PANTR		162
Uncharacterized protein		RABIT		160
Protein max		FELCA		158
Uncharacterized protein		MONDO		151
+69

Protein	Similar proteins		Species	Score	Length	Source
P28574	Protein max	)	HUMAN		160	UniRef50_P61244
Protein max		FELCA		160
Protein max		RAT		160
Protein max		DANRE		165
Protein max		CHICK		160
+426

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M63903 mRNA Translation: AAA39797.1 AK080431 mRNA Translation: BAC37914.1 AC124556 Genomic DNA No translation available. BC138671 mRNA Translation: AAI38672.1 BC138672 mRNA Translation: AAI38673.1 BC145369 mRNA Translation: AAI45370.1
CCDSⁱ	CCDS36479.1 [P28574-1] CCDS49094.1 [P28574-2]
PIRⁱ	A38488
RefSeqⁱ	NP_001139648.1, NM_001146176.1 [P28574-2] NP_032584.2, NM_008558.2 [P28574-1]
UniGeneⁱ	Mm.268548 Mm.491131

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1A93	NMR	-	B	74-102	[»]
	2A93	NMR	-	B	74-102	[»]
	3U5V	X-ray	1.70	A	22-36	[»]
ProteinModelPortalⁱ	P28574
SMRⁱ	P28574
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-105 Transcriptional repressor Mad-Max complex CPX-97 Transcriptional activator Myc-Max complex
CORUMⁱ	P28574
DIPⁱ	DIP-116N
IntActⁱ	P28574, 78 interactors
MINTⁱ	P28574
STRINGⁱ	10090.ENSMUSP00000106025

PTM databases

iPTMnetⁱ	P28574
PhosphoSitePlusⁱ	P28574

Proteomic databases

EPDⁱ	P28574
MaxQBⁱ	P28574
PaxDbⁱ	P28574
PeptideAtlasⁱ	P28574
PRIDEⁱ	P28574

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000082136; ENSMUSP00000080778; ENSMUSG00000059436 [P28574-2] ENSMUST00000110395; ENSMUSP00000106025; ENSMUSG00000059436 [P28574-1]
GeneIDⁱ	17187
KEGGⁱ	mmu:17187
UCSCⁱ	uc007nyw.2 mouse uc007nyx.2 mouse

Organism-specific databases

CTDⁱ	4149
MGIⁱ	MGI:96921 Max

Phylogenomic databases

eggNOGⁱ	KOG2483 Eukaryota ENOG4111V1C LUCA
GeneTreeⁱ	ENSGT00530000064011
HOGENOMⁱ	HOG000293257
HOVERGENⁱ	HBG008542
InParanoidⁱ	P28574
KOⁱ	K04453
OMAⁱ	YTNRKGS
OrthoDBⁱ	EOG091G1312
PhylomeDBⁱ	P28574
TreeFamⁱ	TF318841

Enzyme and pathway databases

Reactomeⁱ	R-MMU-8953750 Transcriptional Regulation by E2F6

Reactomeⁱ

R-MMU-8953750 Transcriptional Regulation by E2F6

Miscellaneous databases

ChiTaRSⁱ	Max mouse
EvolutionaryTraceⁱ	P28574
Protein Ontology More...PROⁱ	PR:P28574
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000059436 Expressed in 246 organ(s), highest expression level in small intestine
CleanExⁱ	MM_MAX

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR037933 MAX
PANTHERⁱ	PTHR10328 PTHR10328, 1 hit
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MAX_MOUSE
Accessionⁱ	P28574Primary (citable) accession number: P28574 Secondary accession number(s): B2RS19, Q8C4Y1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
	Last sequence update:	May 23, 2018
	Last modified:	November 7, 2018
	This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P28574 (MAX_MOUSE)

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Protein max

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ