UniProtKB - P28574 (MAX_MOUSE)
Protein max
Max
Functioni
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. CpG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC:MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements (By similarity).
By similarityGO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor binding Source: Ensembl
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: UniProtKB
- E-box binding Source: MGI
- identical protein binding Source: MGI
- protein-containing complex binding Source: MGI
- protein heterodimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cellular response to peptide hormone stimulus Source: Ensembl
- cellular response to starvation Source: Ensembl
- negative regulation of gene expression Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- neuron apoptotic process Source: Ensembl
- positive regulation of transcription, DNA-templated Source: ComplexPortal
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- protein-containing complex assembly Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: NTNU_SB
- response to axon injury Source: Ensembl
- response to insulin Source: Ensembl
- retina development in camera-type eye Source: Ensembl
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-8953750, Transcriptional Regulation by E2F6 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein maxBy similarityAlternative name(s): Myc-associated factor XBy similarity Myc-binding novel HLH/LZ protein Protein myn |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96921, Max |
VEuPathDBi | HostDB:ENSMUSG00000059436 |
Subcellular locationi
Nucleus
- Mad-Max complex Source: Ensembl
- MLL1 complex Source: UniProtKB
- Myc-Max complex Source: ComplexPortal
- nucleus Source: MGI
- PML body Source: MGI
- RNA polymerase II transcription regulator complex Source: MGI
Other locations
- chromatin Source: MGI
- cytoplasm Source: MGI
- dendrite Source: MGI
- protein-DNA complex Source: MGI
Keywords - Cellular componenti
Cell projection, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000127270 | 2 – 160 | Protein maxAdd BLAST | 159 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 11 | PhosphoserineBy similarity | 1 | |
Modified residuei | 66 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 107 | PhosphoserineBy similarity | 1 | |
Modified residuei | 153 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 154 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P28574 |
jPOSTi | P28574 |
MaxQBi | P28574 |
PaxDbi | P28574 |
PeptideAtlasi | P28574 |
PRIDEi | P28574 |
ProteomicsDBi | 293415 [P28574-1] 328864 340525 |
PTM databases
iPTMneti | P28574 |
PhosphoSitePlusi | P28574 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000059436, Expressed in secondary oocyte and 270 other tissues |
ExpressionAtlasi | P28574, baseline and differential |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MYC or MAD. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2.
Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10.
Interacts with SPAG9. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity.
2 PublicationsBinary interactionsi
P28574
With | #Exp. | IntAct |
---|---|---|
Ccnt1 [Q9QWV9] | 2 | EBI-1183003,EBI-2655009 |
Cdk9 [Q99J95] | 2 | EBI-1183003,EBI-2654963 |
Myc [P01108] | 7 | EBI-1183003,EBI-1183114 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: Ensembl
- identical protein binding Source: MGI
- protein heterodimerization activity Source: InterPro
Protein-protein interaction databases
ComplexPortali | CPX-105, Transcriptional repressor Mad-Max complex CPX-97, Transcriptional activator Myc-Max complex |
CORUMi | P28574 |
DIPi | DIP-116N |
IntActi | P28574, 79 interactors |
MINTi | P28574 |
STRINGi | 10090.ENSMUSP00000106025 |
Miscellaneous databases
RNActi | P28574, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P28574 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28574 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 23 – 74 | bHLHPROSITE-ProRule annotationAdd BLAST | 52 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 81 – 102 | Leucine-zipperAdd BLAST | 22 | |
Regioni | 104 – 160 | DisorderedSequence analysisAdd BLAST | 57 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 13 – 40 | Basic and acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 104 – 128 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 145 – 160 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00530000064011 |
HOGENOMi | CLU_109424_1_0_1 |
InParanoidi | P28574 |
OMAi | QIQTNYS |
OrthoDBi | 1545748at2759 |
PhylomeDBi | P28574 |
TreeFami | TF318841 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
IDEALi | IID50243 |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR037933, MAX |
PANTHERi | PTHR10328, PTHR10328, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSDNDDIEVE SDEEQPRFQS AADKRAHHNA LERKRRDHIK DSFHSLRDSV
60 70 80 90 100
PSLQGEKASR AQILDKATEY IQYMRRKNHT HQQDIDDLKR QNALLEQQVR
110 120 130 140 150
ALEKARSSAQ LQTNYPSSDN SLYTNAKGGT ISAFDGGSDS SSESEPEEPQ
160
SRKKLRMEAS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P7P4 | A0A1W2P7P4_MOUSE | Protein max | Max | 124 | Annotation score: | ||
A0A1W2P702 | A0A1W2P702_MOUSE | Protein max | Max | 14 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | P → A in AAA39797 (PubMed:1840505). | 1 | |
Sequence conflicti | 79 | H → D in AAA39797 (PubMed:1840505). | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059578 | 13 – 21 | Missing in isoform 2. Curated | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63903 mRNA Translation: AAA39797.1 AK080431 mRNA Translation: BAC37914.1 AC124556 Genomic DNA No translation available. BC138671 mRNA Translation: AAI38672.1 BC138672 mRNA Translation: AAI38673.1 BC145369 mRNA Translation: AAI45370.1 |
CCDSi | CCDS36479.1 [P28574-1] CCDS49094.1 [P28574-2] |
PIRi | A38488 |
RefSeqi | NP_001139648.1, NM_001146176.1 [P28574-2] NP_032584.2, NM_008558.2 [P28574-1] |
Genome annotation databases
Ensembli | ENSMUST00000082136; ENSMUSP00000080778; ENSMUSG00000059436 [P28574-2] ENSMUST00000110395; ENSMUSP00000106025; ENSMUSG00000059436 [P28574-1] |
GeneIDi | 17187 |
KEGGi | mmu:17187 |
UCSCi | uc007nyw.2, mouse uc007nyx.2, mouse |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63903 mRNA Translation: AAA39797.1 AK080431 mRNA Translation: BAC37914.1 AC124556 Genomic DNA No translation available. BC138671 mRNA Translation: AAI38672.1 BC138672 mRNA Translation: AAI38673.1 BC145369 mRNA Translation: AAI45370.1 |
CCDSi | CCDS36479.1 [P28574-1] CCDS49094.1 [P28574-2] |
PIRi | A38488 |
RefSeqi | NP_001139648.1, NM_001146176.1 [P28574-2] NP_032584.2, NM_008558.2 [P28574-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A93 | NMR | - | B | 74-102 | [»] | |
2A93 | NMR | - | B | 74-102 | [»] | |
3U5V | X-ray | 1.70 | A | 22-36 | [»] | |
SMRi | P28574 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-105, Transcriptional repressor Mad-Max complex CPX-97, Transcriptional activator Myc-Max complex |
CORUMi | P28574 |
DIPi | DIP-116N |
IntActi | P28574, 79 interactors |
MINTi | P28574 |
STRINGi | 10090.ENSMUSP00000106025 |
PTM databases
iPTMneti | P28574 |
PhosphoSitePlusi | P28574 |
Proteomic databases
EPDi | P28574 |
jPOSTi | P28574 |
MaxQBi | P28574 |
PaxDbi | P28574 |
PeptideAtlasi | P28574 |
PRIDEi | P28574 |
ProteomicsDBi | 293415 [P28574-1] 328864 340525 |
Protocols and materials databases
Antibodypediai | 159, 565 antibodies from 39 providers |
DNASUi | 17187 |
Genome annotation databases
Ensembli | ENSMUST00000082136; ENSMUSP00000080778; ENSMUSG00000059436 [P28574-2] ENSMUST00000110395; ENSMUSP00000106025; ENSMUSG00000059436 [P28574-1] |
GeneIDi | 17187 |
KEGGi | mmu:17187 |
UCSCi | uc007nyw.2, mouse uc007nyx.2, mouse |
Organism-specific databases
CTDi | 4149 |
MGIi | MGI:96921, Max |
VEuPathDBi | HostDB:ENSMUSG00000059436 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00530000064011 |
HOGENOMi | CLU_109424_1_0_1 |
InParanoidi | P28574 |
OMAi | QIQTNYS |
OrthoDBi | 1545748at2759 |
PhylomeDBi | P28574 |
TreeFami | TF318841 |
Enzyme and pathway databases
Reactomei | R-MMU-8953750, Transcriptional Regulation by E2F6 |
Miscellaneous databases
BioGRID-ORCSi | 17187, 22 hits in 64 CRISPR screens |
ChiTaRSi | Max, mouse |
EvolutionaryTracei | P28574 |
PROi | PR:P28574 |
RNActi | P28574, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000059436, Expressed in secondary oocyte and 270 other tissues |
ExpressionAtlasi | P28574, baseline and differential |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
IDEALi | IID50243 |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR037933, MAX |
PANTHERi | PTHR10328, PTHR10328, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAX_MOUSE | |
Accessioni | P28574Primary (citable) accession number: P28574 Secondary accession number(s): B2RS19, Q8C4Y1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | May 23, 2018 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families