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Protein

Protein max

Gene

Max

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. CpG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC:MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyi

Protein namesi
Recommended name:
Protein maxBy similarity
Alternative name(s):
Myc-associated factor XBy similarity
Myc-binding novel HLH/LZ protein
Protein myn
Gene namesi
Name:MaxImported
Synonyms:Myn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:96921 Max

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001272702 – 160Protein maxAdd BLAST159

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei11PhosphoserineBy similarity1
Modified residuei66N6-acetyllysineBy similarity1
Modified residuei107PhosphoserineBy similarity1
Modified residuei153N6-acetyllysineBy similarity1
Modified residuei154N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated.Curated

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP28574
MaxQBiP28574
PaxDbiP28574
PeptideAtlasiP28574
PRIDEiP28574

PTM databases

iPTMnetiP28574
PhosphoSitePlusiP28574

Expressioni

Inductioni

By serum; in 3T3 fibroblasts.

Gene expression databases

BgeeiENSMUSG00000059436 Expressed in 246 organ(s), highest expression level in small intestine
CleanExiMM_MAX

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MYC or MAD. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with SPAG9. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-105 Transcriptional repressor Mad-Max complex
CPX-97 Transcriptional activator Myc-Max complex
CORUMiP28574
DIPiDIP-116N
IntActiP28574, 78 interactors
MINTiP28574
STRINGi10090.ENSMUSP00000106025

Structurei

Secondary structure

1160
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP28574
SMRiP28574
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28574

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 74bHLHPROSITE-ProRule annotationAdd BLAST52

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 102Leucine-zipperAdd BLAST22

Sequence similaritiesi

Belongs to the MAX family.Curated

Phylogenomic databases

eggNOGiKOG2483 Eukaryota
ENOG4111V1C LUCA
GeneTreeiENSGT00530000064011
HOGENOMiHOG000293257
HOVERGENiHBG008542
InParanoidiP28574
KOiK04453
OMAiYTNRKGS
OrthoDBiEOG091G1312
PhylomeDBiP28574
TreeFamiTF318841

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR037933 MAX
PANTHERiPTHR10328 PTHR10328, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P28574-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDNDDIEVE SDEEQPRFQS AADKRAHHNA LERKRRDHIK DSFHSLRDSV
60 70 80 90 100
PSLQGEKASR AQILDKATEY IQYMRRKNHT HQQDIDDLKR QNALLEQQVR
110 120 130 140 150
ALEKARSSAQ LQTNYPSSDN SLYTNAKGGT ISAFDGGSDS SSESEPEEPQ
160
SRKKLRMEAS
Length:160
Mass (Da):18,245
Last modified:May 23, 2018 - v2
Checksum:i86D133137727A57A
GO
Isoform 2 (identifier: P28574-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     13-21: Missing.

Show »
Length:151
Mass (Da):17,172
Checksum:iA6A836F5F39793EF
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P7P4A0A1W2P7P4_MOUSE
Protein max
Max
124Annotation score:
A0A1W2P702A0A1W2P702_MOUSE
Protein max
Max
14Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16P → A in AAA39797 (PubMed:1840505).1
Sequence conflicti79H → D in AAA39797 (PubMed:1840505).1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05957813 – 21Missing in isoform 2. Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63903 mRNA Translation: AAA39797.1
AK080431 mRNA Translation: BAC37914.1
AC124556 Genomic DNA No translation available.
BC138671 mRNA Translation: AAI38672.1
BC138672 mRNA Translation: AAI38673.1
BC145369 mRNA Translation: AAI45370.1
CCDSiCCDS36479.1 [P28574-1]
CCDS49094.1 [P28574-2]
PIRiA38488
RefSeqiNP_001139648.1, NM_001146176.1 [P28574-2]
NP_032584.2, NM_008558.2 [P28574-1]
UniGeneiMm.268548
Mm.491131

Genome annotation databases

EnsembliENSMUST00000082136; ENSMUSP00000080778; ENSMUSG00000059436 [P28574-2]
ENSMUST00000110395; ENSMUSP00000106025; ENSMUSG00000059436 [P28574-1]
GeneIDi17187
KEGGimmu:17187
UCSCiuc007nyw.2 mouse
uc007nyx.2 mouse

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63903 mRNA Translation: AAA39797.1
AK080431 mRNA Translation: BAC37914.1
AC124556 Genomic DNA No translation available.
BC138671 mRNA Translation: AAI38672.1
BC138672 mRNA Translation: AAI38673.1
BC145369 mRNA Translation: AAI45370.1
CCDSiCCDS36479.1 [P28574-1]
CCDS49094.1 [P28574-2]
PIRiA38488
RefSeqiNP_001139648.1, NM_001146176.1 [P28574-2]
NP_032584.2, NM_008558.2 [P28574-1]
UniGeneiMm.268548
Mm.491131

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A93NMR-B74-102[»]
2A93NMR-B74-102[»]
3U5VX-ray1.70A22-36[»]
ProteinModelPortaliP28574
SMRiP28574
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-105 Transcriptional repressor Mad-Max complex
CPX-97 Transcriptional activator Myc-Max complex
CORUMiP28574
DIPiDIP-116N
IntActiP28574, 78 interactors
MINTiP28574
STRINGi10090.ENSMUSP00000106025

PTM databases

iPTMnetiP28574
PhosphoSitePlusiP28574

Proteomic databases

EPDiP28574
MaxQBiP28574
PaxDbiP28574
PeptideAtlasiP28574
PRIDEiP28574

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082136; ENSMUSP00000080778; ENSMUSG00000059436 [P28574-2]
ENSMUST00000110395; ENSMUSP00000106025; ENSMUSG00000059436 [P28574-1]
GeneIDi17187
KEGGimmu:17187
UCSCiuc007nyw.2 mouse
uc007nyx.2 mouse

Organism-specific databases

CTDi4149
MGIiMGI:96921 Max

Phylogenomic databases

eggNOGiKOG2483 Eukaryota
ENOG4111V1C LUCA
GeneTreeiENSGT00530000064011
HOGENOMiHOG000293257
HOVERGENiHBG008542
InParanoidiP28574
KOiK04453
OMAiYTNRKGS
OrthoDBiEOG091G1312
PhylomeDBiP28574
TreeFamiTF318841

Enzyme and pathway databases

ReactomeiR-MMU-8953750 Transcriptional Regulation by E2F6

Miscellaneous databases

ChiTaRSiMax mouse
EvolutionaryTraceiP28574
PROiPR:P28574
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059436 Expressed in 246 organ(s), highest expression level in small intestine
CleanExiMM_MAX

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR037933 MAX
PANTHERiPTHR10328 PTHR10328, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAX_MOUSE
AccessioniPrimary (citable) accession number: P28574
Secondary accession number(s): B2RS19, Q8C4Y1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: May 23, 2018
Last modified: November 7, 2018
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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