UniProtKB - P28565 (5HT1D_RAT)
Protein
5-hydroxytryptamine receptor 1D
Gene
Htr1d
Organism
Rattus norvegicus (Rat)
Status
Functioni
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.2 Publications
GO - Molecular functioni
- G protein-coupled receptor activity Source: GO_Central
- G protein-coupled serotonin receptor activity Source: UniProtKB
- neurotransmitter receptor activity Source: GO_Central
- serotonin binding Source: RGD
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: UniProtKB
- adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: GO_Central
- chemical synaptic transmission Source: GO_Central
- G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
- intestine smooth muscle contraction Source: RGD
- regulation of behavior Source: InterPro
- regulation of locomotion Source: InterPro
- response to toxic substance Source: RGD
- vasoconstriction Source: InterPro
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-390666, Serotonin receptors R-RNO-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: 5-hydroxytryptamine receptor 1DShort name: 5-HT-1D Short name: 5-HT1D Alternative name(s): Serotonin receptor 1D |
Gene namesi | Name:Htr1d |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2847, Htr1d |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Other locations
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 35 | ExtracellularBy similarityAdd BLAST | 35 | |
Transmembranei | 36 – 61 | Helical; Name=1By similarityAdd BLAST | 26 | |
Topological domaini | 62 – 72 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 73 – 95 | Helical; Name=2By similarityAdd BLAST | 23 | |
Topological domaini | 96 – 109 | ExtracellularBy similarityAdd BLAST | 14 | |
Transmembranei | 110 – 131 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 132 – 151 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 152 – 173 | Helical; Name=4By similarityAdd BLAST | 22 | |
Topological domaini | 174 – 191 | ExtracellularBy similarityAdd BLAST | 18 | |
Transmembranei | 192 – 214 | Helical; Name=5By similarityAdd BLAST | 23 | |
Topological domaini | 215 – 299 | CytoplasmicBy similarityAdd BLAST | 85 | |
Transmembranei | 300 – 323 | Helical; Name=6By similarityAdd BLAST | 24 | |
Topological domaini | 324 – 332 | ExtracellularBy similarity | 9 | |
Transmembranei | 333 – 357 | Helical; Name=7By similarityAdd BLAST | 25 | |
Topological domaini | 358 – 374 | CytoplasmicBy similarityAdd BLAST | 17 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068931 | 1 – 374 | 5-hydroxytryptamine receptor 1DAdd BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 5 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 17 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 21 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 108 ↔ 185 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P28565 |
PRIDEi | P28565 |
PTM databases
GlyGeni | P28565, 3 sites |
PhosphoSitePlusi | P28565 |
Expressioni
Tissue specificityi
Detected in dorsal raphe.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000012038, Expressed in brain and 6 other tissues |
Genevisiblei | P28565, RN |
Interactioni
Subunit structurei
Homodimer. Heterodimer with HTR1B (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000016047 |
Chemistry databases
BindingDBi | P28565 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 111 – 120 | Agonist bindingBy similarity | 10 | |
Regioni | 311 – 315 | Agonist bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 132 – 134 | DRY motif; important for ligand-induced conformation changesBy similarity | 3 | |
Motifi | 349 – 353 | NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity | 5 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222287 |
HOGENOMi | CLU_009579_11_1_1 |
InParanoidi | P28565 |
OMAi | ITFCTAS |
OrthoDBi | 703991at2759 |
PhylomeDBi | P28565 |
TreeFami | TF316350 |
Family and domain databases
InterProi | View protein in InterPro IPR000505, 5HT1D_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF17, PTHR24247:SF17, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00514, 5HT1DRECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P28565-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLPNQSLEG LPQEASNRSL NATGAWDPEV LQALRISLVV VLSIITLATV
60 70 80 90 100
LSNAFVLTTI LLTKKLHTPA NYLIGSLATT DLLVSILVMP ISIAYTTTRT
110 120 130 140 150
WNFGQILCDI WVSSDITCCT ASILHLCVIA LDRYWAITDA LEYSKRRTAG
160 170 180 190 200
HAAAMIAAVW AISICISIPP LFWRQATAHE EMSDCLVNTS QISYTIYSTC
210 220 230 240 250
GAFYIPSILL IILYGRIYVA ARSRILNPPS LYGKRFTTAQ LITGSAGSSL
260 270 280 290 300
CSLNPSLHES HTHTVGSPLF FNQVKIKLAD SILERKRISA ARERKATKTL
310 320 330 340 350
GIILGAFIIC WLPFFVVSLV LPICRDSCWI HPALFDFFTW LGYLNSLINP
360 370
VIYTVFNEDF RQAFQRVVHF RKAS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M89953 Genomic DNA Translation: AAA40614.1 |
PIRi | I77467 |
RefSeqi | NP_036984.1, NM_012852.1 XP_008762430.1, XM_008764208.2 XP_017448642.1, XM_017593153.1 XP_017448643.1, XM_017593154.1 XP_017448644.1, XM_017593155.1 XP_017448645.1, XM_017593156.1 XP_017448646.1, XM_017593157.1 XP_017448647.1, XM_017593158.1 |
Genome annotation databases
Ensembli | ENSRNOT00000016046; ENSRNOP00000016047; ENSRNOG00000012038 |
GeneIDi | 25323 |
KEGGi | rno:25323 |
UCSCi | RGD:2847, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M89953 Genomic DNA Translation: AAA40614.1 |
PIRi | I77467 |
RefSeqi | NP_036984.1, NM_012852.1 XP_008762430.1, XM_008764208.2 XP_017448642.1, XM_017593153.1 XP_017448643.1, XM_017593154.1 XP_017448644.1, XM_017593155.1 XP_017448645.1, XM_017593156.1 XP_017448646.1, XM_017593157.1 XP_017448647.1, XM_017593158.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000016047 |
Chemistry databases
BindingDBi | P28565 |
ChEMBLi | CHEMBL5450 |
DrugCentrali | P28565 |
GuidetoPHARMACOLOGYi | 3 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P28565, 3 sites |
PhosphoSitePlusi | P28565 |
Proteomic databases
PaxDbi | P28565 |
PRIDEi | P28565 |
Genome annotation databases
Ensembli | ENSRNOT00000016046; ENSRNOP00000016047; ENSRNOG00000012038 |
GeneIDi | 25323 |
KEGGi | rno:25323 |
UCSCi | RGD:2847, rat |
Organism-specific databases
CTDi | 3352 |
RGDi | 2847, Htr1d |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222287 |
HOGENOMi | CLU_009579_11_1_1 |
InParanoidi | P28565 |
OMAi | ITFCTAS |
OrthoDBi | 703991at2759 |
PhylomeDBi | P28565 |
TreeFami | TF316350 |
Enzyme and pathway databases
Reactomei | R-RNO-390666, Serotonin receptors R-RNO-418594, G alpha (i) signalling events |
Miscellaneous databases
PROi | PR:P28565 |
Gene expression databases
Bgeei | ENSRNOG00000012038, Expressed in brain and 6 other tissues |
Genevisiblei | P28565, RN |
Family and domain databases
InterProi | View protein in InterPro IPR000505, 5HT1D_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF17, PTHR24247:SF17, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00514, 5HT1DRECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 5HT1D_RAT | |
Accessioni | P28565Primary (citable) accession number: P28565 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries