CALR

Functionⁱ

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (By similarity). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.By similarity1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	26	Calcium; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	62	Calcium; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	64	Calcium; via carbonyl oxygenBy similarity	1
Binding siteⁱ	109	CarbohydrateBy similarity	1
Binding siteⁱ	111	CarbohydrateBy similarity	1
Binding siteⁱ	128	CarbohydrateBy similarity	1
Binding siteⁱ	135	CarbohydrateBy similarity	1
Binding siteⁱ	317	CarbohydrateBy similarity	1
Metal bindingⁱ	328	CalciumBy similarity	1

GO - Molecular functionⁱ

androgen receptor binding Source: Ensembl
calcium ion binding Source: UniProtKB
carbohydrate binding Source: UniProtKB-KW
integrin binding Source: Ensembl
mRNA binding Source: Ensembl
ubiquitin protein ligase binding Source: Ensembl
unfolded protein binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular senescence Source: Ensembl
cortical actin cytoskeleton organization Source: Ensembl
negative regulation of cell cycle arrest Source: Ensembl
negative regulation of intracellular steroid hormone receptor signaling pathway Source: Ensembl
negative regulation of neuron differentiation Source: Ensembl
negative regulation of retinoic acid receptor signaling pathway Source: Ensembl
negative regulation of transcription by RNA polymerase II Source: Ensembl
negative regulation of translation Source: Ensembl
negative regulation of trophoblast cell migration Source: Ensembl
peptide antigen assembly with MHC class I protein complex Source: Ensembl
positive regulation of cell population proliferation Source: Ensembl
positive regulation of dendritic cell chemotaxis Source: Ensembl
positive regulation of endothelial cell migration Source: Ensembl
positive regulation of gene expression Source: Ensembl
positive regulation of NIK/NF-kappaB signaling Source: Ensembl
positive regulation of phagocytosis Source: Ensembl
positive regulation of substrate adhesion-dependent cell spreading Source: Ensembl
protein export from nucleus Source: Ensembl
protein folding Source: InterPro
protein localization to nucleus Source: Ensembl
protein stabilization Source: UniProtKB
regulation of meiotic nuclear division Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chaperone
Ligand	Calcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-SSC-1236974 ER-Phagosome pathway R-SSC-3000480 Scavenging by Class A Receptors R-SSC-901042 Calnexin/calreticulin cycle R-SSC-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Reactomeⁱ

R-SSC-1236974 ER-Phagosome pathway
R-SSC-3000480 Scavenging by Class A Receptors
R-SSC-901042 Calnexin/calreticulin cycle
R-SSC-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Calreticulin Alternative name(s): CRP55 Calregulin Endoplasmic reticulum resident protein 60 Short name: ERp60 HACBP
Gene namesⁱ	Name:CALR
Organismⁱ	Sus scrofa (Pig)
Taxonomic identifierⁱ	9823 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus
Proteomesⁱ	UP000008227 Componentⁱ: Chromosome 2

Keywords - Cellular componentⁱ

Cytoplasm, Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 17	1 PublicationAdd BLAST		17
ChainⁱPRO_0000004175	18 – 417	CalreticulinAdd BLAST		400

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	137 ↔ 163	By similarity
Modified residueⁱ	159	N6-acetyllysineBy similarity	1
Modified residueⁱ	209	N6-acetyllysineBy similarity	1

Keywords - PTMⁱ

Acetylation, Disulfide bond

Proteomic databases

PaxDbⁱ	P28491
PeptideAtlas More...PeptideAtlasⁱ	P28491
PRIDEⁱ	P28491

Expressionⁱ

Tissue specificityⁱ

In blastocyst expressed in all blastomeres (at protein level). In embryos, expressed in spleen, kidney, liver, fat, muscle, ovary, granulosa cells and cumulus cells.1 Publication

Developmental stageⁱ

Expressed in immature GV-stage oocytes, mature MII-stage oocytes, parthenogenetically activated MII-stage oocytes and in pronuclear embryos (at protein level). During in vitro oocyte maturation, expression initially increases after 12 hours of culture, followed by a strong decline at 30 hours (MI stage) and 44 hours (MII stage). Expression remains constant in MII-stage oocytes after parthenogenetic activation, increasing in two- and four-cell parthenotes. Not detected in blastocyst stage embryos.1 Publication

Gene expression databases

Bgeeⁱ	ENSSSCG00000013746 Expressed in 6 organ(s), highest expression level in liver
ExpressionAtlasⁱ	P28491 baseline and differential
Genevisibleⁱ	P28491 SS

Interactionⁱ

Subunit structureⁱ

Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with GABARAP, NR3C1, PDIA3/ERp57 and TRIM21. Interacts with PPIB and SPACA9. Interacts (via P-domain) with PDIA5 (By similarity).By similarity

GO - Molecular functionⁱ

androgen receptor binding Source: Ensembl
integrin binding Source: Ensembl
ubiquitin protein ligase binding Source: Ensembl
unfolded protein binding Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	9823.ENSSSCP00000014615

STRINGⁱ

9823.ENSSSCP00000014615

Structureⁱ

3D structure databases

SMRⁱ	P28491
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	191 – 202	1-1Add BLAST	12
Repeatⁱ	210 – 221	1-2Add BLAST	12
Repeatⁱ	227 – 238	1-3Add BLAST	12
Repeatⁱ	244 – 255	1-4Add BLAST	12
Repeatⁱ	259 – 269	2-1Add BLAST	11
Repeatⁱ	273 – 283	2-2Add BLAST	11
Repeatⁱ	287 – 297	2-3Add BLAST	11

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	18 – 197	N-domainAdd BLAST	180
Regionⁱ	191 – 255	4 X approximate repeatsAdd BLAST	65
Regionⁱ	198 – 308	P-domainAdd BLAST	111
Regionⁱ	237 – 270	Interaction with PPIBBy similarityAdd BLAST	34
Regionⁱ	259 – 297	3 X approximate repeatsAdd BLAST	39
Regionⁱ	309 – 417	C-domainAdd BLAST	109

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	414 – 417	Prevents secretion from ERPROSITE-ProRule annotation		4

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	351 – 407	Asp/Glu/Lys-richAdd BLAST		57

Domainⁱ

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity

The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity

The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesⁱ

Belongs to the calreticulin family.Curated

Keywords - Domainⁱ

Repeat, Signal

Phylogenomic databases

eggNOGⁱ	KOG0674 Eukaryota ENOG410XRR7 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00950000182915
HOGENOMⁱ	HOG000192435
InParanoidⁱ	P28491
KEGG Orthology (KO) More...KOⁱ	K08057
OMAⁱ	DTWIYSK
Database of Orthologous Groups More...OrthoDBⁱ	822188at2759
TreeFamⁱ	TF338438

Family and domain databases

Gene3Dⁱ	2.10.250.10, 2 hits
InterProⁱ	View protein in InterPro IPR001580 Calret/calnex IPR018124 Calret/calnex_CS IPR009169 Calreticulin IPR009033 Calreticulin/calnexin_P_dom_sf IPR013320 ConA-like_dom_sf
PANTHERⁱ	PTHR11073 PTHR11073, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00262 Calreticulin, 2 hits
PIRSFⁱ	PIRSF002356 Calreticulin, 1 hit
PRINTSⁱ	PR00626 CALRETICULIN
SUPFAMⁱ	SSF49899 SSF49899, 1 hit SSF63887 SSF63887, 1 hit
PROSITEⁱ	View protein in PROSITE PS00803 CALRETICULIN_1, 1 hit PS00804 CALRETICULIN_2, 1 hit PS00805 CALRETICULIN_REPEAT, 3 hits PS00014 ER_TARGET, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

P28491-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLLPVPLLLG LVGLAAAEPT IYFKEQFLDG DGWTDRWIES KHKPDFGRFV 
        60         70         80         90        100
LSSGKFYGDQ EKDKGLQTSQ DARFYALSAR FEPFSNKGQT LVVQFTVKHE 
       110        120        130        140        150
QNIDCGGGYV KLFPDGLDQT DMHGDSEYNI MFGPDICGPG TKKVHVIFNY 
       160        170        180        190        200
KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW 
       210        220        230        240        250
DFLPPKKIKD PDAVKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP 
       260        270        280        290        300
EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS 
       310        320        330        340        350
PDSNIYAYEN FAVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT 
       360        370        380        390        400
KAAEKQMKDK QDEEQRLKEE EEEKKRKEEE EVDKEDEEDK DEDEEEEDEK 
       410 
EEEEEEDAAA GQAKDEL

Length:417

Mass (Da):48,288

Last modified:May 16, 2012 - v3

Checksum:ⁱ31A9E4CC95F2D54F

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A287AG29	A0A287AG29_PIG	Calreticulin Calreticulin	CALR	383	Annotation score:
A0A287B0P6	A0A287B0P6_PIG	Calreticulin Calreticulin	CALR	369	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	15	A → V in CAA23142 (PubMed:8672129).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	GQ984146 mRNA Translation: ADD52600.1 CU463133 Genomic DNA No translation available. F14591 mRNA Translation: CAA23142.1
PIRⁱ	B33208
NCBI Reference Sequences More...RefSeqⁱ	NP_001167604.1, NM_001174133.1

Genome annotation databases

Ensemblⁱ	ENSSSCT00000015020; ENSSSCP00000014615; ENSSSCG00000013746
GeneIDⁱ	100381266
KEGGⁱ	ssc:100381266

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P28491	Calreticulin	)	HUMAN		417	UniRef90_P27797
CALR isoform 2		PONAB		417
Uncharacterized protein		Cebus capucinus imitator		424
Uncharacterized protein		AOTNA		424
Calreticulin		RABIT		418
+104

Protein	Similar proteins		Species	Score	Length	Source
P28491	Calreticulin	)	HUMAN		417	UniRef50_P27797
CALR isoform 2		PONAB		417
Uncharacterized protein		Cebus capucinus imitator		424
Uncharacterized protein		AOTNA		424
Calreticulin		RABIT		418
+425

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	GQ984146 mRNA Translation: ADD52600.1 CU463133 Genomic DNA No translation available. F14591 mRNA Translation: CAA23142.1
PIRⁱ	B33208
RefSeqⁱ	NP_001167604.1, NM_001174133.1

3D structure databases

SMRⁱ	P28491
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	9823.ENSSSCP00000014615

STRINGⁱ

9823.ENSSSCP00000014615

Proteomic databases

PaxDbⁱ	P28491
PeptideAtlasⁱ	P28491
PRIDEⁱ	P28491

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSSSCT00000015020; ENSSSCP00000014615; ENSSSCG00000013746
GeneIDⁱ	100381266
KEGGⁱ	ssc:100381266

Organism-specific databases

CTDⁱ	811

CTDⁱ

811

Phylogenomic databases

eggNOGⁱ	KOG0674 Eukaryota ENOG410XRR7 LUCA
GeneTreeⁱ	ENSGT00950000182915
HOGENOMⁱ	HOG000192435
InParanoidⁱ	P28491
KOⁱ	K08057
OMAⁱ	DTWIYSK
OrthoDBⁱ	822188at2759
TreeFamⁱ	TF338438

Enzyme and pathway databases

Reactomeⁱ	R-SSC-1236974 ER-Phagosome pathway R-SSC-3000480 Scavenging by Class A Receptors R-SSC-901042 Calnexin/calreticulin cycle R-SSC-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Reactomeⁱ

R-SSC-1236974 ER-Phagosome pathway
R-SSC-3000480 Scavenging by Class A Receptors
R-SSC-901042 Calnexin/calreticulin cycle
R-SSC-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Gene expression databases

Bgeeⁱ	ENSSSCG00000013746 Expressed in 6 organ(s), highest expression level in liver
ExpressionAtlasⁱ	P28491 baseline and differential
Genevisibleⁱ	P28491 SS

Family and domain databases

Gene3Dⁱ	2.10.250.10, 2 hits
InterProⁱ	View protein in InterPro IPR001580 Calret/calnex IPR018124 Calret/calnex_CS IPR009169 Calreticulin IPR009033 Calreticulin/calnexin_P_dom_sf IPR013320 ConA-like_dom_sf
PANTHERⁱ	PTHR11073 PTHR11073, 1 hit
Pfamⁱ	View protein in Pfam PF00262 Calreticulin, 2 hits
PIRSFⁱ	PIRSF002356 Calreticulin, 1 hit
PRINTSⁱ	PR00626 CALRETICULIN
SUPFAMⁱ	SSF49899 SSF49899, 1 hit SSF63887 SSF63887, 1 hit
PROSITEⁱ	View protein in PROSITE PS00803 CALRETICULIN_1, 1 hit PS00804 CALRETICULIN_2, 1 hit PS00805 CALRETICULIN_REPEAT, 3 hits PS00014 ER_TARGET, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CALR_PIG
Accessionⁱ	P28491Primary (citable) accession number: P28491 Secondary accession number(s): D4N5N1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
	Last sequence update:	May 16, 2012
	Last modified:	May 8, 2019
	This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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UniParc

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Supporting data

UniProtKB - P28491 (CALR_PIG)

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Calreticulin

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Other locations

Cytosol

Endoplasmic reticulum

Extracellular region or secreted

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

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Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ