UniProtKB - P28491 (CALR_PIG)
Protein
Calreticulin
Gene
CALR
Organism
Sus scrofa (Pig)
Status
Functioni
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (By similarity). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.By similarity1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 26 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 62 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 64 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 109 | CarbohydrateBy similarity | 1 | |
Binding sitei | 111 | CarbohydrateBy similarity | 1 | |
Binding sitei | 128 | CarbohydrateBy similarity | 1 | |
Binding sitei | 135 | CarbohydrateBy similarity | 1 | |
Binding sitei | 317 | CarbohydrateBy similarity | 1 | |
Metal bindingi | 328 | CalciumBy similarity | 1 |
GO - Molecular functioni
- androgen receptor binding Source: Ensembl
- calcium ion binding Source: UniProtKB
- carbohydrate binding Source: UniProtKB-KW
- integrin binding Source: Ensembl
- mRNA binding Source: Ensembl
- ubiquitin protein ligase binding Source: Ensembl
- unfolded protein binding Source: InterPro
GO - Biological processi
- cellular senescence Source: Ensembl
- cortical actin cytoskeleton organization Source: Ensembl
- endoplasmic reticulum unfolded protein response Source: GO_Central
- negative regulation of cell cycle arrest Source: Ensembl
- negative regulation of intracellular steroid hormone receptor signaling pathway Source: Ensembl
- negative regulation of neuron differentiation Source: Ensembl
- negative regulation of retinoic acid receptor signaling pathway Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- negative regulation of translation Source: Ensembl
- negative regulation of trophoblast cell migration Source: Ensembl
- peptide antigen assembly with MHC class I protein complex Source: Ensembl
- positive regulation of cell population proliferation Source: Ensembl
- positive regulation of dendritic cell chemotaxis Source: Ensembl
- positive regulation of endothelial cell migration Source: Ensembl
- positive regulation of gene expression Source: Ensembl
- positive regulation of NIK/NF-kappaB signaling Source: Ensembl
- positive regulation of phagocytosis Source: Ensembl
- positive regulation of substrate adhesion-dependent cell spreading Source: Ensembl
- protein export from nucleus Source: Ensembl
- protein folding Source: GO_Central
- protein localization to nucleus Source: Ensembl
- protein stabilization Source: UniProtKB
- regulation of meiotic nuclear division Source: Ensembl
Keywordsi
Molecular function | Chaperone |
Ligand | Calcium, Lectin, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SSC-1236974, ER-Phagosome pathway R-SSC-3000480, Scavenging by Class A Receptors R-SSC-901042, Calnexin/calreticulin cycle R-SSC-983170, Antigen Presentation: Folding, assembly and peptide loading of class I MHC |
Names & Taxonomyi
Protein namesi | Recommended name: CalreticulinAlternative name(s): CRP55 Calregulin Endoplasmic reticulum resident protein 60 Short name: ERp60 HACBP |
Gene namesi | Name:CALR |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:86149, CALR |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen 1 Publication
Other locations
- Sarcoplasmic reticulum lumen 1 Publication
- perinuclear region 1 Publication
- Membrane 1 Publication
Note: During oocyte maturation and after parthenogenetic activation accumulates in the plasma membrane region. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (PubMed:20222029).1 Publication
Cytosol
- cytosol Source: Ensembl
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
- endoplasmic reticulum quality control compartment Source: Ensembl
- MHC class I peptide loading complex Source: Ensembl
- sarcoplasmic reticulum lumen Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular space Source: Ensembl
Nucleus
- nuclear envelope Source: Ensembl
Plasma Membrane
- external side of plasma membrane Source: Ensembl
Other locations
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- polysome Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Membrane, Sarcoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | 1 PublicationAdd BLAST | 17 | |
ChainiPRO_0000004175 | 18 – 417 | CalreticulinAdd BLAST | 400 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 137 ↔ 163 | By similarity | ||
Modified residuei | 159 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 209 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, Disulfide bondProteomic databases
PaxDbi | P28491 |
PeptideAtlasi | P28491 |
PRIDEi | P28491 |
Expressioni
Tissue specificityi
In blastocyst expressed in all blastomeres (at protein level). In embryos, expressed in spleen, kidney, liver, fat, muscle, ovary, granulosa cells and cumulus cells.1 Publication
Developmental stagei
Expressed in immature GV-stage oocytes, mature MII-stage oocytes, parthenogenetically activated MII-stage oocytes and in pronuclear embryos (at protein level). During in vitro oocyte maturation, expression initially increases after 12 hours of culture, followed by a strong decline at 30 hours (MI stage) and 44 hours (MII stage). Expression remains constant in MII-stage oocytes after parthenogenetic activation, increasing in two- and four-cell parthenotes. Not detected in blastocyst stage embryos.1 Publication
Gene expression databases
Bgeei | ENSSSCG00000013746, Expressed in uterus and 37 other tissues |
ExpressionAtlasi | P28491, baseline and differential |
Genevisiblei | P28491, SS |
Interactioni
Subunit structurei
Monomer.
Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.
Interacts with GABARAP, NR3C1, PDIA3/ERp57 and TRIM21.
Interacts with PPIB and SPACA9.
Interacts (via P-domain) with PDIA5 (By similarity).
Interacts with CLCC1 (By similarity).
By similarityGO - Molecular functioni
- androgen receptor binding Source: Ensembl
- integrin binding Source: Ensembl
- ubiquitin protein ligase binding Source: Ensembl
- unfolded protein binding Source: InterPro
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000014615 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 191 – 202 | 1-1Add BLAST | 12 | |
Repeati | 210 – 221 | 1-2Add BLAST | 12 | |
Repeati | 227 – 238 | 1-3Add BLAST | 12 | |
Repeati | 244 – 255 | 1-4Add BLAST | 12 | |
Repeati | 259 – 269 | 2-1Add BLAST | 11 | |
Repeati | 273 – 283 | 2-2Add BLAST | 11 | |
Repeati | 287 – 297 | 2-3Add BLAST | 11 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 18 – 197 | N-domainAdd BLAST | 180 | |
Regioni | 191 – 255 | 4 X approximate repeatsAdd BLAST | 65 | |
Regioni | 198 – 308 | P-domainAdd BLAST | 111 | |
Regioni | 237 – 270 | Interaction with PPIBBy similarityAdd BLAST | 34 | |
Regioni | 259 – 297 | 3 X approximate repeatsAdd BLAST | 39 | |
Regioni | 309 – 417 | C-domainAdd BLAST | 109 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 414 – 417 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 351 – 407 | Asp/Glu/Lys-richAdd BLAST | 57 |
Domaini
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity
Sequence similaritiesi
Belongs to the calreticulin family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG0674, Eukaryota |
GeneTreei | ENSGT00950000182915 |
HOGENOMi | CLU_018224_0_2_1 |
InParanoidi | P28491 |
OMAi | VGFELWQ |
OrthoDBi | 822188at2759 |
TreeFami | TF338438 |
Family and domain databases
Gene3Di | 2.10.250.10, 2 hits |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009169, Calreticulin IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 2 hits |
PIRSFi | PIRSF002356, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 3 hits PS00014, ER_TARGET, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P28491-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLPVPLLLG LVGLAAAEPT IYFKEQFLDG DGWTDRWIES KHKPDFGRFV
60 70 80 90 100
LSSGKFYGDQ EKDKGLQTSQ DARFYALSAR FEPFSNKGQT LVVQFTVKHE
110 120 130 140 150
QNIDCGGGYV KLFPDGLDQT DMHGDSEYNI MFGPDICGPG TKKVHVIFNY
160 170 180 190 200
KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW
210 220 230 240 250
DFLPPKKIKD PDAVKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP
260 270 280 290 300
EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS
310 320 330 340 350
PDSNIYAYEN FAVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT
360 370 380 390 400
KAAEKQMKDK QDEEQRLKEE EEEKKRKEEE EVDKEDEEDK DEDEEEEDEK
410
EEEEEEDAAA GQAKDEL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A287B0P6 | A0A287B0P6_PIG | Calreticulin | CALR | 369 | Annotation score: | ||
A0A287AG29 | A0A287AG29_PIG | Calreticulin | CALR | 400 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | A → V in CAA23142 (PubMed:8672129).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ984146 mRNA Translation: ADD52600.1 CU463133 Genomic DNA No translation available. F14591 mRNA Translation: CAA23142.1 |
PIRi | B33208 |
RefSeqi | NP_001167604.1, NM_001174133.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ984146 mRNA Translation: ADD52600.1 CU463133 Genomic DNA No translation available. F14591 mRNA Translation: CAA23142.1 |
PIRi | B33208 |
RefSeqi | NP_001167604.1, NM_001174133.1 |
3D structure databases
BMRBi | P28491 |
SMRi | P28491 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000014615 |
Proteomic databases
PaxDbi | P28491 |
PeptideAtlasi | P28491 |
PRIDEi | P28491 |
Genome annotation databases
Organism-specific databases
CTDi | 811 |
VGNCi | VGNC:86149, CALR |
Phylogenomic databases
eggNOGi | KOG0674, Eukaryota |
GeneTreei | ENSGT00950000182915 |
HOGENOMi | CLU_018224_0_2_1 |
InParanoidi | P28491 |
OMAi | VGFELWQ |
OrthoDBi | 822188at2759 |
TreeFami | TF338438 |
Enzyme and pathway databases
Reactomei | R-SSC-1236974, ER-Phagosome pathway R-SSC-3000480, Scavenging by Class A Receptors R-SSC-901042, Calnexin/calreticulin cycle R-SSC-983170, Antigen Presentation: Folding, assembly and peptide loading of class I MHC |
Gene expression databases
Bgeei | ENSSSCG00000013746, Expressed in uterus and 37 other tissues |
ExpressionAtlasi | P28491, baseline and differential |
Genevisiblei | P28491, SS |
Family and domain databases
Gene3Di | 2.10.250.10, 2 hits |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009169, Calreticulin IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 2 hits |
PIRSFi | PIRSF002356, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 3 hits PS00014, ER_TARGET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CALR_PIG | |
Accessioni | P28491Primary (citable) accession number: P28491 Secondary accession number(s): D4N5N1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | May 16, 2012 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families