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Protein

Protein Wnt-5

Gene

Wnt5

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May have a role in limb and CNS development; may be a downstream target of Dll that acts in the specification of these primordia.2 Publications

GO - Molecular functioni

  • frizzled binding Source: GO_Central
  • signaling receptor binding Source: FlyBase

GO - Biological processi

  • axon extension Source: FlyBase
  • axon guidance Source: FlyBase
  • cell fate commitment Source: GO_Central
  • chemorepulsion of axon Source: FlyBase
  • dendrite guidance Source: FlyBase
  • determination of muscle attachment site Source: FlyBase
  • negative chemotaxis Source: FlyBase
  • positive regulation of axon extension Source: FlyBase
  • positive regulation of axon guidance Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • Wnt signaling pathway Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-DME-3238698 WNT ligand biogenesis and trafficking
R-DME-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
SignaLinkiP28466

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-5
Alternative name(s):
dWnt-3
dWnt-5
Gene namesi
Name:Wnt5
Synonyms:Wnt-3, WNT-5
ORF Names:CG6407
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0010194 Wnt5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000004147930 – 1004Protein Wnt-5Add BLAST975

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi115N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi307N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi341N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi422N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi484N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi485N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi528N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi583 ↔ 594By similarity
Glycosylationi593N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi633 ↔ 641By similarity
Disulfide bondi643 ↔ 661By similarity
Glycosylationi724N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi862 ↔ 876By similarity
Disulfide bondi864 ↔ 871By similarity
Lipidationi868O-palmitoleoyl serine; by PORCNBy similarity1
Disulfide bondi949 ↔ 964By similarity
Glycosylationi952N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi979 ↔ 994By similarity
Disulfide bondi981 ↔ 991By similarity
Disulfide bondi986 ↔ 987By similarity

Post-translational modificationi

Glycosylated, glycosylation is stimulated by porcupine at the ER.1 Publication
Palmitoleoylated by porcupine. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport Wnt5 to unique sites at the cell surface. Depalmitoleoylated by notum, leading to inhibit Wnt signaling pathway.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiP28466
PRIDEiP28466

Expressioni

Tissue specificityi

Dynamic expression pattern during embryogenesis. Expression is seen in the limb primordia of the head and thoracic segments, mesodermal and neurogenic regions.2 Publications

Gene expression databases

BgeeiFBgn0010194
ExpressionAtlasiP28466 baseline and differential
GenevisibleiP28466 DM

Interactioni

Subunit structurei

Interacts with porcupine (por).1 Publication

GO - Molecular functioni

  • frizzled binding Source: GO_Central
  • signaling receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi59151, 16 interactors
DIPiDIP-22455N
IntActiP28466, 8 interactors
MINTiP28466
STRINGi7227.FBpp0074394

Structurei

3D structure databases

ProteinModelPortaliP28466
SMRiP28466
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi436 – 470Ser-richAdd BLAST35
Compositional biasi959 – 994Cys-richAdd BLAST36

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
InParanoidiP28466
KOiK00444
OMAiRFEPIIQ
OrthoDBiEOG091G0OFF
PhylomeDBiP28466

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 2 hits
PRINTSiPR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCYRKRHFL LWLLRAVCML HLTARGAYAT VGLQGVPTWI YLGLKSPFIE
60 70 80 90 100
FGNQVEQLAN SSIPLNMTKD EQANMHQEGL RKLGTFIKPV DLRDSETGFV
110 120 130 140 150
KADLTKRLVF DRPNNITSRP IHPIQEEMDQ KQIILLDEDT DENGLPASLT
160 170 180 190 200
DEDRKFIVPM ALKNISPDPR WAATTPSPSA LQPNAKAIST IVPSPLAQVE
210 220 230 240 250
GDPTSNIDDL KKHILFLHNM TKTNSNFESK FVKFPSLQKD KAKTSGAGGS
260 270 280 290 300
PPNPKRPQRP IHQYSAPIAP PTPKVPAPDG GGVGGAAYNP GEQPIGGYYQ
310 320 330 340 350
NEELANNQSL LKPTDTDSHP AAGGSSHGQK NPSEPQVILL NETLSTETSI
360 370 380 390 400
EADRSPSINQ PKAGSPARTT KRPPCLRNPE SPKCIRQRRR EEQQRQRERD
410 420 430 440 450
EWFRGQSQYM QPRFEPIIQT INNTKRFAVS IEIPDSFKVS SEGSDGELLS
460 470 480 490 500
RVERSQPSIS SSSSSSSSSS RKIMPDYIKV SMENNTSVTD YFKHDVVMTS
510 520 530 540 550
ADVASDREFL IKNMEEHGGA GSANSHHNDT TPTADAYSET IDLNPNNCYS
560 570 580 590 600
AIGLSNSQKK QCVKHTSVMP AISRGARAAI QECQFQFKNR RWNCSTTNDE
610 620 630 640 650
TVFGPMTSLA APEMAFIHAL AAATVTSFIA RACRDGQLAS CSCSRGSRPK
660 670 680 690 700
QLHDDWKWGG CGDNLEFAYK FATDFIDSRE KETNRETRGV KRKREEINKN
710 720 730 740 750
RMHSDDTNAF NIGIKRNKNV DAKNDTSLVV RNVRKSTEAE NSHILNENFD
760 770 780 790 800
QHLLELEQRI TKEILTSKID EEEMIKLQEK IKQEIVNTKF FKGEQQPRKK
810 820 830 840 850
KRKNQRAAAD APAYPRNGIK ESYKDGGILP RSTATVKARS LMNLHNNEAG
860 870 880 890 900
RRAVIKKARI TCKCHGVSGS CSLITCWQQL SSIREIGDYL REKYEGATKV
910 920 930 940 950
KINKRGRLQI KDLQFKVPTA HDLIYLDESP DWCRNSYALH WPGTHGRVCH
960 970 980 990 1000
KNSSGLESCA ILCCGRGYNT KNIIVNERCN CKFHWCCQVK CEVCTKVLEE

HTCK
Length:1,004
Mass (Da):112,459
Last modified:March 1, 2004 - v2
Checksum:i4E2BB4DC7165CAA5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti282G → GGGG (PubMed:1425336).Curated1
Sequence conflicti317D → E in CAA46002 (PubMed:1425336).Curated1
Sequence conflicti470S → SSSS (PubMed:1425336).Curated1
Sequence conflicti525S → G (PubMed:1425336).Curated1
Sequence conflicti525S → G (PubMed:1358729).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64736 mRNA Translation: CAA46002.1
M97450 mRNA Translation: AAA29020.1
AE014298 Genomic DNA Translation: AAF48853.1
BT010268 mRNA Translation: AAQ23586.1
PIRiA48821
RefSeqiNP_001285407.1, NM_001298478.1
NP_476924.1, NM_057576.5
UniGeneiDm.5375

Genome annotation databases

EnsemblMetazoaiFBtr0074623; FBpp0074394; FBgn0010194
FBtr0343046; FBpp0309792; FBgn0010194
GeneIDi32838
KEGGidme:Dmel_CG6407

Similar proteinsi

Entry informationi

Entry nameiWNT5_DROME
AccessioniPrimary (citable) accession number: P28466
Secondary accession number(s): Q01535, Q9VWT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: March 1, 2004
Last modified: July 18, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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