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UniProtKB - P28367 (RF2_BACSU)
Protein
Peptide chain release factor 2
Gene
prfB
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.
Miscellaneous
The gene for this protein contains a UGA in-frame termination codon after Leu-24; a naturally occurring frameshift enables complete translation of RF-2. This provides a mechanism for the protein to regulate its own production.
GO - Molecular functioni
- translation release factor activity, codon specific Source: UniProtKB-UniRule
Keywordsi
Biological process | Protein biosynthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU35290-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Peptide chain release factor 2Short name: RF-2 |
Gene namesi | Name:prfB Ordered Locus Names:BSU35290 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166803 | 1 – 366 | Peptide chain release factor 2Add BLAST | 366 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 251 | N5-methylglutamineBy similarity | 1 |
Post-translational modificationi
Methylated by PrmC. Methylation increases the termination efficiency of RF2 (By similarity).By similarity
Keywords - PTMi
MethylationProteomic databases
PaxDbi | P28367 |
PRIDEi | P28367 |
Family & Domainsi
Sequence similaritiesi
Belongs to the prokaryotic/mitochondrial release factor family.Curated
Phylogenomic databases
eggNOGi | COG1186, Bacteria |
InParanoidi | P28367 |
OMAi | YVFHPYQ |
PhylomeDBi | P28367 |
Family and domain databases
HAMAPi | MF_00094, Rel_fac_2, 1 hit |
InterProi | View protein in InterPro IPR005139, PCRF IPR000352, Pep_chain_release_fac_I IPR045853, Pep_chain_release_fac_I_sf IPR004374, PrfB |
Pfami | View protein in Pfam PF03462, PCRF, 1 hit PF00472, RF-1, 1 hit |
SMARTi | View protein in SMART SM00937, PCRF, 1 hit |
SUPFAMi | SSF75620, SSF75620, 1 hit |
TIGRFAMsi | TIGR00020, prfB, 1 hit |
PROSITEi | View protein in PROSITE PS00745, RF_PROK_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P28367-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELSEIRAEL ENMASRLADF RGSLDLESKE ARIAELDEQM ADPEFWNDQQ
60 70 80 90 100
KAQTVINEAN GLKDYVNSYK KLNESHEELQ MTHDLLKEEP DTDLQLELEK
110 120 130 140 150
ELKSLTKEFN EFELQLLLSE PYDKNNAILE LHPGAGGTES QDWGSMLLRM
160 170 180 190 200
YTRWGERRGF KVETLDYLPG DEAGIKSVTL LIKGHNAYGY LKAEKGVHRL
210 220 230 240 250
VRISPFDSSG RRHTSFVSCE VMPEFNDEID IDIRTEDIKV DTYRASGAGG
260 270 280 290 300
QHVNTTDSAV RITHLPTNVV VTCQTERSQI KNRERAMKML KAKLYQRRIE
310 320 330 340 350
EQQAELDEIR GEQKEIGWGS QIRSYVFHPY SMVKDHRTNT EMGNVQAVMD
360
GDIDTFIDAY LRSKLS
Sequence cautioni
The sequence BAA01123 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 338 | T → D in BAA01123 (PubMed:1901557).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF013188 Genomic DNA Translation: AAC97534.1 AF017113 Genomic DNA Translation: AAC67303.1 AL009126 Genomic DNA Translation: CAB15546.1 D10279 Genomic DNA Translation: BAA01123.1 Different initiation. |
PIRi | H69681, JN0146 |
RefSeqi | NP_391409.1, NC_000964.3 WP_010886623.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15546; CAB15546; BSU_35290 |
GeneIDi | 936692 |
KEGGi | bsu:BSU35290 |
PATRICi | fig|224308.43.peg.3694 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF013188 Genomic DNA Translation: AAC97534.1 AF017113 Genomic DNA Translation: AAC67303.1 AL009126 Genomic DNA Translation: CAB15546.1 D10279 Genomic DNA Translation: BAA01123.1 Different initiation. |
PIRi | H69681, JN0146 |
RefSeqi | NP_391409.1, NC_000964.3 WP_010886623.1, NZ_JNCM01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6SZS | electron microscopy | 3.06 | z | 5-366 | [»] | |
SMRi | P28367 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU35290 |
Proteomic databases
PaxDbi | P28367 |
PRIDEi | P28367 |
Genome annotation databases
EnsemblBacteriai | CAB15546; CAB15546; BSU_35290 |
GeneIDi | 936692 |
KEGGi | bsu:BSU35290 |
PATRICi | fig|224308.43.peg.3694 |
Phylogenomic databases
eggNOGi | COG1186, Bacteria |
InParanoidi | P28367 |
OMAi | YVFHPYQ |
PhylomeDBi | P28367 |
Enzyme and pathway databases
BioCyci | BSUB:BSU35290-MONOMER |
Family and domain databases
HAMAPi | MF_00094, Rel_fac_2, 1 hit |
InterProi | View protein in InterPro IPR005139, PCRF IPR000352, Pep_chain_release_fac_I IPR045853, Pep_chain_release_fac_I_sf IPR004374, PrfB |
Pfami | View protein in Pfam PF03462, PCRF, 1 hit PF00472, RF-1, 1 hit |
SMARTi | View protein in SMART SM00937, PCRF, 1 hit |
SUPFAMi | SSF75620, SSF75620, 1 hit |
TIGRFAMsi | TIGR00020, prfB, 1 hit |
PROSITEi | View protein in PROSITE PS00745, RF_PROK_I, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RF2_BACSU | |
Accessioni | P28367Primary (citable) accession number: P28367 Secondary accession number(s): O34444 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families