UniProtKB - P28366 (SECA_BACSU)
Protein translocase subunit SecA
secA
Functioni
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.
UniRule annotationCatalytic activityi
- ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2).UniRule annotation1 Publication EC:7.4.2.8
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 85 | ATPUniRule annotationCombined sources4 Publications | 1 | |
Binding sitei | 492 | ATPUniRule annotationCombined sources3 Publications | 1 | |
Metal bindingi | 825 | ZincUniRule annotation | 1 | |
Metal bindingi | 827 | ZincUniRule annotation | 1 | |
Metal bindingi | 836 | ZincUniRule annotation | 1 | |
Metal bindingi | 837 | ZincUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 79 – 80 | ATPCombined sources2 Publications | 2 | |
Nucleotide bindingi | 103 – 107 | ATPUniRule annotationCombined sources4 Publications | 5 |
GO - Molecular functioni
- ABC-type protein transporter activity Source: GO_Central
- ATP binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- protein-exporting ATPase activity Source: UniProtKB-EC
GO - Biological processi
- intracellular protein transmembrane transport Source: UniProtKB-UniRule
- protein import Source: InterPro
- protein targeting Source: UniProtKB-UniRule
- protein transport by the Sec complex Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Protein transport, Translocation, Transport |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | BSUB:BSU35300-MONOMER |
BRENDAi | 7.4.2.5, 658 |
Protein family/group databases
TCDBi | 3.A.5.2.1, the general secretory pathway (sec) family |
Names & Taxonomyi
Protein namesi | Recommended name: Protein translocase subunit SecAUniRule annotation (EC:7.4.2.8UniRule annotation1 Publication) |
Gene namesi | Name:secAUniRule annotation Synonyms:div+ Ordered Locus Names:BSU35300 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Plasma membrane
- Cell membrane UniRule annotation1 Publication; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Other locations
- Membrane raft 1 Publication
Note: Distribution is 50-50.UniRule annotation
Cytosol
- cytosol Source: GO_Central
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Other locations
- cell envelope Sec protein transport complex Source: GO_Central
- membrane raft Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 101 | K → N: Can restore growth of E.coli secA mutants. 1 Publication | 1 | |
Mutagenesisi | 106 | K → N: Loss of activity. Cannot complement E.coli secA mutants. 1 Publication | 1 | |
Mutagenesisi | 587 | G → C: Forms position 587-750 dimers upon oxidation in vitro; when associated with C-750. Does not form position 587-587 dimers (homodimers). 1 Publication | 1 | |
Mutagenesisi | 588 | N → C: Forms position 588-588 dimers upon oxidation in vitro (homodimers). 1 Publication | 1 | |
Mutagenesisi | 750 | R → C: Forms position 587-750 dimers upon oxidation in vitro; when associated with C-587. Also forms position 750-750 dimers (homodimers). 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109576 | 1 – 841 | Protein translocase subunit SecAAdd BLAST | 841 |
Proteomic databases
jPOSTi | P28366 |
PaxDbi | P28366 |
PRIDEi | P28366 |
Interactioni
Subunit structurei
Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may be involved. Monomer and many different homodimers can be isolated (PubMed:16989859), some of which are not formed in the presence of a synthetic signal peptide. A single SecA monomer interacts with SecY in the channel. Only shows some colocalization with FloA or FloT membrane assemblies (PubMed:27362352).
3 PublicationsProtein-protein interaction databases
DIPi | DIP-59805N |
IntActi | P28366, 7 interactors |
MINTi | P28366 |
STRINGi | 224308.BSU35300 |
Structurei
Secondary structure
3D structure databases
SMRi | P28366 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28366 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 786 – 813 | DisorderedSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0653, Bacteria |
InParanoidi | P28366 |
OMAi | MVHYDVQ |
PhylomeDBi | P28366 |
Family and domain databases
CDDi | cd18803, SF2_C_secA, 1 hit |
Gene3Di | 3.40.50.300, 3 hits |
HAMAPi | MF_01382, SecA, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR004027, SEC_C_motif IPR000185, SecA IPR020937, SecA_CS IPR011115, SecA_DEAD IPR014018, SecA_motor_DEAD IPR011130, SecA_preprotein_X-link_dom IPR044722, SecA_SF2_C IPR011116, SecA_Wing/Scaffold IPR036266, SecA_Wing/Scaffold_sf IPR036670, SecA_X-link_sf |
PANTHERi | PTHR30612, PTHR30612, 1 hit |
Pfami | View protein in Pfam PF02810, SEC-C, 1 hit PF07517, SecA_DEAD, 1 hit PF01043, SecA_PP_bind, 1 hit PF07516, SecA_SW, 1 hit |
PRINTSi | PR00906, SECA |
SMARTi | View protein in SMART SM00957, SecA_DEAD, 1 hit SM00958, SecA_PP_bind, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF81767, SSF81767, 1 hit SSF81886, SSF81886, 1 hit |
TIGRFAMsi | TIGR00963, secA, 1 hit |
PROSITEi | View protein in PROSITE PS01312, SECA, 1 hit PS51196, SECA_MOTOR_DEAD, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLGILNKMFD PTKRTLNRYE KIANDIDAIR GDYENLSDDA LKHKTIEFKE
60 70 80 90 100
RLEKGATTDD LLVEAFAVVR EASRRVTGMF PFKVQLMGGV ALHDGNIAEM
110 120 130 140 150
KTGEGKTLTS TLPVYLNALT GKGVHVVTVN EYLASRDAEQ MGKIFEFLGL
160 170 180 190 200
TVGLNLNSMS KDEKREAYAA DITYSTNNEL GFDYLRDNMV LYKEQMVQRP
210 220 230 240 250
LHFAVIDEVD SILIDEARTP LIISGQAAKS TKLYVQANAF VRTLKAEKDY
260 270 280 290 300
TYDIKTKAVQ LTEEGMTKAE KAFGIDNLFD VKHVALNHHI NQALKAHVAM
310 320 330 340 350
QKDVDYVVED GQVVIVDSFT GRLMKGRRYS EGLHQAIEAK EGLEIQNESM
360 370 380 390 400
TLATITFQNY FRMYEKLAGM TGTAKTEEEE FRNIYNMQVV TIPTNRPVVR
410 420 430 440 450
DDRPDLIYRT MEGKFKAVAE DVAQRYMTGQ PVLVGTVAVE TSELISKLLK
460 470 480 490 500
NKGIPHQVLN AKNHEREAQI IEEAGQKGAV TIATNMAGRG TDIKLGEGVK
510 520 530 540 550
ELGGLAVVGT ERHESRRIDN QLRGRSGRQG DPGITQFYLS MEDELMRRFG
560 570 580 590 600
AERTMAMLDR FGMDDSTPIQ SKMVSRAVES SQKRVEGNNF DSRKQLLQYD
610 620 630 640 650
DVLRQQREVI YKQRFEVIDS ENLREIVENM IKSSLERAIA AYTPREELPE
660 670 680 690 700
EWKLDGLVDL INTTYLDEGA LEKSDIFGKE PDEMLELIMD RIITKYNEKE
710 720 730 740 750
EQFGKEQMRE FEKVIVLRAV DSKWMDHIDA MDQLRQGIHL RAYAQTNPLR
760 770 780 790 800
EYQMEGFAMF EHMIESIEDE VAKFVMKAEI ENNLEREEVV QGQTTAHQPQ
810 820 830 840
EGDDNKKAKK APVRKVVDIG RNAPCHCGSG KKYKNCCGRT E
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 126 | V → I in CAA43977 (PubMed:1832735).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10279 Genomic DNA Translation: BAA01122.1 U56901 Genomic DNA Translation: AAC44957.1 AL009126 Genomic DNA Translation: CAB15547.1 X62035 Genomic DNA Translation: CAA43977.1 |
PIRi | JQ0647 |
RefSeqi | NP_391410.1, NC_000964.3 WP_003228033.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15547; CAB15547; BSU_35300 |
GeneIDi | 936711 |
KEGGi | bsu:BSU35300 |
PATRICi | fig|224308.179.peg.3820 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10279 Genomic DNA Translation: BAA01122.1 U56901 Genomic DNA Translation: AAC44957.1 AL009126 Genomic DNA Translation: CAB15547.1 X62035 Genomic DNA Translation: CAA43977.1 |
PIRi | JQ0647 |
RefSeqi | NP_391410.1, NC_000964.3 WP_003228033.1, NZ_JNCM01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M6N | X-ray | 2.70 | A | 1-802 | [»] | |
1M74 | X-ray | 3.00 | A | 1-802 | [»] | |
1TF2 | X-ray | 2.90 | A | 1-841 | [»] | |
1TF5 | X-ray | 2.18 | A | 1-841 | [»] | |
2IBM | X-ray | 3.20 | A/B | 1-780 | [»] | |
3DL8 | X-ray | 7.50 | A/B | 1-779 | [»] | |
3IQM | X-ray | 3.40 | A | 1-802 | [»] | |
3IQY | X-ray | 3.30 | A | 1-841 | [»] | |
3JV2 | X-ray | 2.50 | A/B | 1-780 | [»] | |
5EUL | X-ray | 3.70 | A | 1-780 | [»] | |
6ITC | electron microscopy | 3.45 | A | 1-780 | [»] | |
SMRi | P28366 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-59805N |
IntActi | P28366, 7 interactors |
MINTi | P28366 |
STRINGi | 224308.BSU35300 |
Protein family/group databases
TCDBi | 3.A.5.2.1, the general secretory pathway (sec) family |
Proteomic databases
jPOSTi | P28366 |
PaxDbi | P28366 |
PRIDEi | P28366 |
Genome annotation databases
EnsemblBacteriai | CAB15547; CAB15547; BSU_35300 |
GeneIDi | 936711 |
KEGGi | bsu:BSU35300 |
PATRICi | fig|224308.179.peg.3820 |
Phylogenomic databases
eggNOGi | COG0653, Bacteria |
InParanoidi | P28366 |
OMAi | MVHYDVQ |
PhylomeDBi | P28366 |
Enzyme and pathway databases
BioCyci | BSUB:BSU35300-MONOMER |
BRENDAi | 7.4.2.5, 658 |
Miscellaneous databases
EvolutionaryTracei | P28366 |
Family and domain databases
CDDi | cd18803, SF2_C_secA, 1 hit |
Gene3Di | 3.40.50.300, 3 hits |
HAMAPi | MF_01382, SecA, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR004027, SEC_C_motif IPR000185, SecA IPR020937, SecA_CS IPR011115, SecA_DEAD IPR014018, SecA_motor_DEAD IPR011130, SecA_preprotein_X-link_dom IPR044722, SecA_SF2_C IPR011116, SecA_Wing/Scaffold IPR036266, SecA_Wing/Scaffold_sf IPR036670, SecA_X-link_sf |
PANTHERi | PTHR30612, PTHR30612, 1 hit |
Pfami | View protein in Pfam PF02810, SEC-C, 1 hit PF07517, SecA_DEAD, 1 hit PF01043, SecA_PP_bind, 1 hit PF07516, SecA_SW, 1 hit |
PRINTSi | PR00906, SECA |
SMARTi | View protein in SMART SM00957, SecA_DEAD, 1 hit SM00958, SecA_PP_bind, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF81767, SSF81767, 1 hit SSF81886, SSF81886, 1 hit |
TIGRFAMsi | TIGR00963, secA, 1 hit |
PROSITEi | View protein in PROSITE PS01312, SECA, 1 hit PS51196, SECA_MOTOR_DEAD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SECA_BACSU | |
Accessioni | P28366Primary (citable) accession number: P28366 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families