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Protein

Protein translocase subunit SecA

Gene

secA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: May bind 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi825ZincUniRule annotation1
Metal bindingi827ZincUniRule annotation1
Metal bindingi836ZincUniRule annotation1
Metal bindingi837ZincUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 107ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU35300-MONOMER
BRENDAi3.6.3.B1 658

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecAUniRule annotation
Gene namesi
Name:secAUniRule annotation
Synonyms:div+
Ordered Locus Names:BSU35300
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi587G → C: Forms position 587-750 dimers upon oxidation in vitro; when associated with C-750. Does not form position 587-587 dimers (homodimers). 1 Publication1
Mutagenesisi588N → C: Forms position 588-588 dimers upon oxidation in vitro (homodimers). 1 Publication1
Mutagenesisi750R → C: Forms position 587-750 dimers upon oxidation in vitro; when associated with C-587. Also forms position 750-750 dimers (homodimers). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001095761 – 841Protein translocase subunit SecAAdd BLAST841

Proteomic databases

PaxDbiP28366
PRIDEiP28366

Interactioni

Subunit structurei

Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may be involved. Monomer and many different homodimers can be isolated (PubMed:16989859), some of which are not formed in the presence of a synthetic signal peptide. A single SecA monomer interacts with SecY in the channel.2 Publications

Protein-protein interaction databases

DIPiDIP-59805N
IntActiP28366, 7 interactors
MINTiP28366
STRINGi224308.Bsubs1_010100019096

Structurei

Secondary structure

1841
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP28366
SMRiP28366
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28366

Family & Domainsi

Sequence similaritiesi

Belongs to the SecA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0653 LUCA
HOGENOMiHOG000218169
InParanoidiP28366
KOiK03070
OMAiFEYDEVM
PhylomeDBiP28366

Family and domain databases

HAMAPiMF_01382 SecA, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR004027 SEC_C_motif
IPR000185 SecA
IPR020937 SecA_CS
IPR011115 SecA_DEAD
IPR014018 SecA_motor_DEAD
IPR011130 SecA_preprotein_X-link_dom
IPR011116 SecA_Wing/Scaffold
IPR036266 SecA_Wing/Scaffold_sf
IPR036670 SecA_X-link_sf
PANTHERiPTHR30612 PTHR30612, 2 hits
PfamiView protein in Pfam
PF02810 SEC-C, 1 hit
PF07517 SecA_DEAD, 1 hit
PF01043 SecA_PP_bind, 1 hit
PF07516 SecA_SW, 1 hit
PRINTSiPR00906 SECA
SMARTiView protein in SMART
SM00957 SecA_DEAD, 1 hit
SM00958 SecA_PP_bind, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
SSF81767 SSF81767, 1 hit
SSF81886 SSF81886, 2 hits
TIGRFAMsiTIGR00963 secA, 1 hit
PROSITEiView protein in PROSITE
PS01312 SECA, 1 hit
PS51196 SECA_MOTOR_DEAD, 1 hit

Sequencei

Sequence statusi: Complete.

P28366-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLGILNKMFD PTKRTLNRYE KIANDIDAIR GDYENLSDDA LKHKTIEFKE
60 70 80 90 100
RLEKGATTDD LLVEAFAVVR EASRRVTGMF PFKVQLMGGV ALHDGNIAEM
110 120 130 140 150
KTGEGKTLTS TLPVYLNALT GKGVHVVTVN EYLASRDAEQ MGKIFEFLGL
160 170 180 190 200
TVGLNLNSMS KDEKREAYAA DITYSTNNEL GFDYLRDNMV LYKEQMVQRP
210 220 230 240 250
LHFAVIDEVD SILIDEARTP LIISGQAAKS TKLYVQANAF VRTLKAEKDY
260 270 280 290 300
TYDIKTKAVQ LTEEGMTKAE KAFGIDNLFD VKHVALNHHI NQALKAHVAM
310 320 330 340 350
QKDVDYVVED GQVVIVDSFT GRLMKGRRYS EGLHQAIEAK EGLEIQNESM
360 370 380 390 400
TLATITFQNY FRMYEKLAGM TGTAKTEEEE FRNIYNMQVV TIPTNRPVVR
410 420 430 440 450
DDRPDLIYRT MEGKFKAVAE DVAQRYMTGQ PVLVGTVAVE TSELISKLLK
460 470 480 490 500
NKGIPHQVLN AKNHEREAQI IEEAGQKGAV TIATNMAGRG TDIKLGEGVK
510 520 530 540 550
ELGGLAVVGT ERHESRRIDN QLRGRSGRQG DPGITQFYLS MEDELMRRFG
560 570 580 590 600
AERTMAMLDR FGMDDSTPIQ SKMVSRAVES SQKRVEGNNF DSRKQLLQYD
610 620 630 640 650
DVLRQQREVI YKQRFEVIDS ENLREIVENM IKSSLERAIA AYTPREELPE
660 670 680 690 700
EWKLDGLVDL INTTYLDEGA LEKSDIFGKE PDEMLELIMD RIITKYNEKE
710 720 730 740 750
EQFGKEQMRE FEKVIVLRAV DSKWMDHIDA MDQLRQGIHL RAYAQTNPLR
760 770 780 790 800
EYQMEGFAMF EHMIESIEDE VAKFVMKAEI ENNLEREEVV QGQTTAHQPQ
810 820 830 840
EGDDNKKAKK APVRKVVDIG RNAPCHCGSG KKYKNCCGRT E
Length:841
Mass (Da):95,531
Last modified:December 1, 1992 - v1
Checksum:i9AAC3630139F5EFF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126V → I in CAA43977 (PubMed:1832735).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10279 Genomic DNA Translation: BAA01122.1
U56901 Genomic DNA Translation: AAC44957.1
AL009126 Genomic DNA Translation: CAB15547.1
X62035 Genomic DNA Translation: CAA43977.1
PIRiJQ0647
RefSeqiNP_391410.1, NC_000964.3
WP_003228033.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15547; CAB15547; BSU35300
GeneIDi936711
KEGGibsu:BSU35300
PATRICifig|224308.179.peg.3820

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10279 Genomic DNA Translation: BAA01122.1
U56901 Genomic DNA Translation: AAC44957.1
AL009126 Genomic DNA Translation: CAB15547.1
X62035 Genomic DNA Translation: CAA43977.1
PIRiJQ0647
RefSeqiNP_391410.1, NC_000964.3
WP_003228033.1, NZ_JNCM01000033.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M6NX-ray2.70A1-802[»]
1M74X-ray3.00A1-802[»]
1TF2X-ray2.90A1-841[»]
1TF5X-ray2.18A1-841[»]
2IBMX-ray3.20A/B1-780[»]
3DL8X-ray7.50A/B1-779[»]
3IQMX-ray3.40A1-802[»]
3IQYX-ray3.30A1-841[»]
3JV2X-ray2.50A/B1-780[»]
5EULX-ray3.70A1-780[»]
ProteinModelPortaliP28366
SMRiP28366
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59805N
IntActiP28366, 7 interactors
MINTiP28366
STRINGi224308.Bsubs1_010100019096

Proteomic databases

PaxDbiP28366
PRIDEiP28366

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15547; CAB15547; BSU35300
GeneIDi936711
KEGGibsu:BSU35300
PATRICifig|224308.179.peg.3820

Phylogenomic databases

eggNOGiCOG0653 LUCA
HOGENOMiHOG000218169
InParanoidiP28366
KOiK03070
OMAiFEYDEVM
PhylomeDBiP28366

Enzyme and pathway databases

BioCyciBSUB:BSU35300-MONOMER
BRENDAi3.6.3.B1 658

Miscellaneous databases

EvolutionaryTraceiP28366

Family and domain databases

HAMAPiMF_01382 SecA, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR004027 SEC_C_motif
IPR000185 SecA
IPR020937 SecA_CS
IPR011115 SecA_DEAD
IPR014018 SecA_motor_DEAD
IPR011130 SecA_preprotein_X-link_dom
IPR011116 SecA_Wing/Scaffold
IPR036266 SecA_Wing/Scaffold_sf
IPR036670 SecA_X-link_sf
PANTHERiPTHR30612 PTHR30612, 2 hits
PfamiView protein in Pfam
PF02810 SEC-C, 1 hit
PF07517 SecA_DEAD, 1 hit
PF01043 SecA_PP_bind, 1 hit
PF07516 SecA_SW, 1 hit
PRINTSiPR00906 SECA
SMARTiView protein in SMART
SM00957 SecA_DEAD, 1 hit
SM00958 SecA_PP_bind, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
SSF81767 SSF81767, 1 hit
SSF81886 SSF81886, 2 hits
TIGRFAMsiTIGR00963 secA, 1 hit
PROSITEiView protein in PROSITE
PS01312 SECA, 1 hit
PS51196 SECA_MOTOR_DEAD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSECA_BACSU
AccessioniPrimary (citable) accession number: P28366
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 10, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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