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Protein

5-hydroxytryptamine receptor 1B

Gene

Htr1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.6 Publications

Miscellaneous

A residue in the 7th transmembrane region ('Thr-355' in human, Asn-351 in mouse and rat) is important for species-specific sensitivity to various agonists.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei351Important for species-specific agonist sensitivityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Enzyme and pathway databases

ReactomeiR-MMU-390666 Serotonin receptors
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1B
Short name:
5-HT-1B
Short name:
5-HT1B
Alternative name(s):
Serotonin receptor 1B
Gene namesi
Name:Htr1b
Synonyms:5ht1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:96274 Htr1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 45ExtracellularBy similarityAdd BLAST45
Transmembranei46 – 71Helical; Name=1By similarityAdd BLAST26
Topological domaini72 – 80CytoplasmicBy similarity9
Transmembranei81 – 106Helical; Name=2By similarityAdd BLAST26
Topological domaini107 – 119ExtracellularBy similarityAdd BLAST13
Transmembranei120 – 141Helical; Name=3By similarityAdd BLAST22
Topological domaini142 – 161CytoplasmicBy similarityAdd BLAST20
Transmembranei162 – 183Helical; Name=4By similarityAdd BLAST22
Topological domaini184 – 201ExtracellularBy similarityAdd BLAST18
Transmembranei202 – 224Helical; Name=5By similarityAdd BLAST23
Topological domaini225 – 311CytoplasmicBy similarityAdd BLAST87
Transmembranei312 – 332Helical; Name=6By similarityAdd BLAST21
Topological domaini333 – 345ExtracellularBy similarityAdd BLAST13
Transmembranei346 – 367Helical; Name=7By similarityAdd BLAST22
Topological domaini368 – 386CytoplasmicBy similarityAdd BLAST19

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant male mice display increased aggressivity towards intruders. Treatment with a Htr1b agonist does not trigger increased locomotor behavior in mutant mice, contrary to what is observed with wild-type mice. Treatment with a Htr1b agonist does not inhibit 5-hydroxytryptamine release in the frontal cortex and hippocampus of mutant mice, contrary to what is observed with wild-type mice. Likewise, Htr1b agonists do not inhibit dopamine and acetylcholine release in brains from mutant mice.5 Publications

Chemistry databases

ChEMBLiCHEMBL3708691
GuidetoPHARMACOLOGYi2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689171 – 3865-hydroxytryptamine receptor 1BAdd BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi28N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi118 ↔ 195PROSITE-ProRule annotation
Lipidationi384S-palmitoyl cysteineSequence analysis1

Post-translational modificationi

Phosphorylated.By similarity
Palmitoylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP28334
PRIDEiP28334

PTM databases

iPTMnetiP28334
PhosphoSitePlusiP28334

Expressioni

Tissue specificityi

Predominantly expressed in striatum and Purkinje cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000049511 Expressed in 150 organ(s), highest expression level in caudate-putamen
CleanExiMM_HTR1B
ExpressionAtlasiP28334 baseline and differential
GenevisibleiP28334 MM

Interactioni

Subunit structurei

Homodimer. Heterodimer with HTR1D (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050898

Chemistry databases

BindingDBiP28334

Structurei

3D structure databases

ProteinModelPortaliP28334
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 130Agonist bindingBy similarity10
Regioni323 – 327Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 144DRY motif; important for ligand-induced conformation changes and signalingBy similarity3
Motifi361 – 365NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118795
HOGENOMiHOG000239242
HOVERGENiHBG106962
InParanoidiP28334
KOiK04153
OMAiVNTDHVL
OrthoDBiEOG091G06VI
PhylomeDBiP28334
TreeFamiTF316350

Family and domain databases

InterProiView protein in InterPro
IPR002147 5HT1B_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF16 PTHR24247:SF16, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00513 5HT1BRECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P28334-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEQGIQCAP PPPAASQTGV PLTNLSHNCS ADGYIYQDSI ALPWKVLLVA
60 70 80 90 100
LLALITLATT LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP
110 120 130 140 150
ISTMYTVTGR WTLGQVVCDF WLSSDITCCT ASIMHLCVIA LDRYWAITDA
160 170 180 190 200
VEYSAKRTPK RAAIMIVLVW VFSISISLPP FFWRQAKAEE EMLDCFVNTD
210 220 230 240 250
HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP NKTGKRLTRA
260 270 280 290 300
QLITDSPGST SSVTSINSRA PDVPSESGSP VYVNQVKVRV SDALLEKKKL
310 320 330 340 350
MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF
360 370 380
NWLGYLNSLI NPIIYTMSNE DFKQAFHKLI RFKCAG
Length:386
Mass (Da):43,079
Last modified:December 1, 1992 - v1
Checksum:i58F70FBEA770C0B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11597 Genomic DNA Translation: CAA77678.1
M85151 Genomic DNA Translation: AAA83221.1
CCDSiCCDS23370.1
PIRiA42688
RefSeqiNP_034612.1, NM_010482.1
UniGeneiMm.445308

Genome annotation databases

EnsembliENSMUST00000051005; ENSMUSP00000050898; ENSMUSG00000049511
ENSMUST00000183482; ENSMUSP00000139389; ENSMUSG00000049511
GeneIDi15551
KEGGimmu:15551
UCSCiuc009qvl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11597 Genomic DNA Translation: CAA77678.1
M85151 Genomic DNA Translation: AAA83221.1
CCDSiCCDS23370.1
PIRiA42688
RefSeqiNP_034612.1, NM_010482.1
UniGeneiMm.445308

3D structure databases

ProteinModelPortaliP28334
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050898

Chemistry databases

BindingDBiP28334
ChEMBLiCHEMBL3708691
GuidetoPHARMACOLOGYi2

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP28334
PhosphoSitePlusiP28334

Proteomic databases

PaxDbiP28334
PRIDEiP28334

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051005; ENSMUSP00000050898; ENSMUSG00000049511
ENSMUST00000183482; ENSMUSP00000139389; ENSMUSG00000049511
GeneIDi15551
KEGGimmu:15551
UCSCiuc009qvl.1 mouse

Organism-specific databases

CTDi3351
MGIiMGI:96274 Htr1b

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118795
HOGENOMiHOG000239242
HOVERGENiHBG106962
InParanoidiP28334
KOiK04153
OMAiVNTDHVL
OrthoDBiEOG091G06VI
PhylomeDBiP28334
TreeFamiTF316350

Enzyme and pathway databases

ReactomeiR-MMU-390666 Serotonin receptors
R-MMU-418594 G alpha (i) signalling events

Miscellaneous databases

PROiPR:P28334
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049511 Expressed in 150 organ(s), highest expression level in caudate-putamen
CleanExiMM_HTR1B
ExpressionAtlasiP28334 baseline and differential
GenevisibleiP28334 MM

Family and domain databases

InterProiView protein in InterPro
IPR002147 5HT1B_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF16 PTHR24247:SF16, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00513 5HT1BRECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5HT1B_MOUSE
AccessioniPrimary (citable) accession number: P28334
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 12, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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