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Protein

Cystatin-D

Gene

CST5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B.1 Publication

Caution

The truncated forms found may result from N-terminal proteolysis.1 Publication

Temperature dependencei

Thermostable.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Reactive siteBy similarity1

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: UniProtKB

Keywordsi

Molecular functionProtease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI25.005

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-D
Alternative name(s):
Cystatin-5
Gene namesi
Name:CST5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000170367.4
HGNCiHGNC:2477 CST5
MIMi123858 gene
neXtProtiNX_P28325

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1473
OpenTargetsiENSG00000170367
PharmGKBiPA26978

Polymorphism and mutation databases

BioMutaiCST5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 201 PublicationAdd BLAST20
ChainiPRO_000000664521 – 142Cystatin-DAdd BLAST122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi95 ↔ 105
Disulfide bondi119 ↔ 139

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP28325
PaxDbiP28325
PeptideAtlasiP28325
PRIDEiP28325
ProteomicsDBi54464

Expressioni

Tissue specificityi

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall bladder tissue.1 Publication

Gene expression databases

BgeeiENSG00000170367 Expressed in 32 organ(s), highest expression level in saliva-secreting gland
CleanExiHS_CST5
GenevisibleiP28325 HS

Organism-specific databases

HPAiCAB024944
HPA061449

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi107855, 24 interactors
STRINGi9606.ENSP00000307132

Structurei

Secondary structure

1142
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP28325
SMRiP28325
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28325

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi70 – 74Secondary area of contact5

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410KIFQ Eukaryota
ENOG4110QA3 LUCA
GeneTreeiENSGT00900000140934
HOGENOMiHOG000231754
HOVERGENiHBG009556
InParanoidiP28325
KOiK13901
OMAiPFNDQPE
OrthoDBiEOG091G0TP1
PhylomeDBiP28325

Family and domain databases

CDDicd00042 CY, 1 hit
InterProiView protein in InterPro
IPR027214 Cystatin
IPR000010 Cystatin_dom
IPR018073 Prot_inh_cystat_CS
PANTHERiPTHR11413 PTHR11413, 1 hit
PfamiView protein in Pfam
PF00031 Cystatin, 1 hit
SMARTiView protein in SMART
SM00043 CY, 1 hit
PROSITEiView protein in PROSITE
PS00287 CYSTATIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28325-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMWPMHTPLL LLTALMVAVA GSASAQSRTL AGGIHATDLN DKSVQCALDF
60 70 80 90 100
AISEYNKVIN KDEYYSRPLQ VMAAYQQIVG GVNYYFNVKF GRTTCTKSQP
110 120 130 140
NLDNCPFNDQ PKLKEEEFCS FQINEVPWED KISILNYKCR KV
Length:142
Mass (Da):16,080
Last modified:December 1, 1992 - v1
Checksum:iCEFA89BA87A0DA68
GO

Mass spectrometryi

Molecular mass is 13154.4675±0.0101 Da from positions 29 - 142. Determined by ESI. Variant Arg-46.1 Publication
Molecular mass is 13596.7015±0.0049 Da from positions 25 - 142. Determined by ESI. Variant Arg-46.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00220846C → R in 45% of the population. 1 PublicationCorresponds to variant dbSNP:rs1799841Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59964 Genomic DNA Translation: CAA42590.1
X70377 mRNA Translation: CAA49838.1
AL591074 Genomic DNA No translation available.
BC062678 mRNA Translation: AAH62678.1
BC069514 mRNA Translation: AAH69514.1
CCDSiCCDS13162.1
PIRiA47142
RefSeqiNP_001891.2, NM_001900.4
UniGeneiHs.121489

Genome annotation databases

EnsembliENST00000304710; ENSP00000307132; ENSG00000170367
GeneIDi1473
KEGGihsa:1473
UCSCiuc002wtr.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59964 Genomic DNA Translation: CAA42590.1
X70377 mRNA Translation: CAA49838.1
AL591074 Genomic DNA No translation available.
BC062678 mRNA Translation: AAH62678.1
BC069514 mRNA Translation: AAH69514.1
CCDSiCCDS13162.1
PIRiA47142
RefSeqiNP_001891.2, NM_001900.4
UniGeneiHs.121489

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RN7X-ray2.50A21-142[»]
1ROAX-ray1.80A21-142[»]
ProteinModelPortaliP28325
SMRiP28325
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107855, 24 interactors
STRINGi9606.ENSP00000307132

Protein family/group databases

MEROPSiI25.005

Polymorphism and mutation databases

BioMutaiCST5

Proteomic databases

EPDiP28325
PaxDbiP28325
PeptideAtlasiP28325
PRIDEiP28325
ProteomicsDBi54464

Protocols and materials databases

DNASUi1473
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304710; ENSP00000307132; ENSG00000170367
GeneIDi1473
KEGGihsa:1473
UCSCiuc002wtr.2 human

Organism-specific databases

CTDi1473
DisGeNETi1473
EuPathDBiHostDB:ENSG00000170367.4
GeneCardsiCST5
HGNCiHGNC:2477 CST5
HPAiCAB024944
HPA061449
MIMi123858 gene
neXtProtiNX_P28325
OpenTargetsiENSG00000170367
PharmGKBiPA26978
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410KIFQ Eukaryota
ENOG4110QA3 LUCA
GeneTreeiENSGT00900000140934
HOGENOMiHOG000231754
HOVERGENiHBG009556
InParanoidiP28325
KOiK13901
OMAiPFNDQPE
OrthoDBiEOG091G0TP1
PhylomeDBiP28325

Miscellaneous databases

EvolutionaryTraceiP28325
GeneWikiiCST5
GenomeRNAii1473
PROiPR:P28325
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170367 Expressed in 32 organ(s), highest expression level in saliva-secreting gland
CleanExiHS_CST5
GenevisibleiP28325 HS

Family and domain databases

CDDicd00042 CY, 1 hit
InterProiView protein in InterPro
IPR027214 Cystatin
IPR000010 Cystatin_dom
IPR018073 Prot_inh_cystat_CS
PANTHERiPTHR11413 PTHR11413, 1 hit
PfamiView protein in Pfam
PF00031 Cystatin, 1 hit
SMARTiView protein in SMART
SM00043 CY, 1 hit
PROSITEiView protein in PROSITE
PS00287 CYSTATIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYTD_HUMAN
AccessioniPrimary (citable) accession number: P28325
Secondary accession number(s): Q5JRF5, Q9UCA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 7, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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