UniProtKB - P28325 (CYTD_HUMAN)
Protein
Cystatin-D
Gene
CST5
Organism
Homo sapiens (Human)
Status
Functioni
Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B.1 Publication
Caution
The truncated forms found may result from N-terminal proteolysis.1 Publication
Temperature dependencei
Thermostable.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 32 | Reactive siteBy similarity | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase inhibitor activity Source: UniProtKB
Keywordsi
Molecular function | Protease inhibitor, Thiol protease inhibitor |
Enzyme and pathway databases
PathwayCommonsi | P28325 |
Protein family/group databases
MEROPSi | I25.005 |
Names & Taxonomyi
Protein namesi | Recommended name: Cystatin-DAlternative name(s): Cystatin-5 |
Gene namesi | Name:CST5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000170367.4 |
HGNCi | HGNC:2477, CST5 |
MIMi | 123858, gene |
neXtProti | NX_P28325 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: UniProtKB
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 1473 |
OpenTargetsi | ENSG00000170367 |
PharmGKBi | PA26978 |
Miscellaneous databases
Pharosi | P28325, Tbio |
Polymorphism and mutation databases
BioMutai | CST5 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | 1 PublicationAdd BLAST | 20 | |
ChainiPRO_0000006645 | 21 – 142 | Cystatin-DAdd BLAST | 122 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 95 ↔ 105 | |||
Disulfide bondi | 119 ↔ 139 |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P28325 |
MassIVEi | P28325 |
PaxDbi | P28325 |
PeptideAtlasi | P28325 |
PRIDEi | P28325 |
ProteomicsDBi | 54464 |
Expressioni
Tissue specificityi
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall bladder tissue.1 Publication
Gene expression databases
Bgeei | ENSG00000170367, Expressed in saliva-secreting gland and 59 other tissues |
Genevisiblei | P28325, HS |
Organism-specific databases
HPAi | ENSG00000170367, Tissue enriched (salivary) |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 107855, 24 interactors |
IntActi | P28325, 7 interactors |
STRINGi | 9606.ENSP00000307132 |
Miscellaneous databases
RNActi | P28325, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28325 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28325 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 70 – 74 | Secondary area of contact | 5 |
Sequence similaritiesi
Belongs to the cystatin family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502SC50, Eukaryota |
GeneTreei | ENSGT00940000164560 |
HOGENOMi | CLU_118168_0_1_1 |
InParanoidi | P28325 |
OMAi | FTIREYN |
OrthoDBi | 1565344at2759 |
PhylomeDBi | P28325 |
Family and domain databases
CDDi | cd00042, CY, 1 hit |
InterProi | View protein in InterPro IPR000010, Cystatin_dom IPR018073, Prot_inh_cystat_CS |
Pfami | View protein in Pfam PF00031, Cystatin, 1 hit |
SMARTi | View protein in SMART SM00043, CY, 1 hit |
PROSITEi | View protein in PROSITE PS00287, CYSTATIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P28325-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMWPMHTPLL LLTALMVAVA GSASAQSRTL AGGIHATDLN DKSVQCALDF
60 70 80 90 100
AISEYNKVIN KDEYYSRPLQ VMAAYQQIVG GVNYYFNVKF GRTTCTKSQP
110 120 130 140
NLDNCPFNDQ PKLKEEEFCS FQINEVPWED KISILNYKCR KV
Mass spectrometryi
Molecular mass is 13154.4675±0.0101 Da. Determined by ESI. Variant Arg-46.1 Publication
Molecular mass is 13596.7015±0.0049 Da. Determined by ESI. Variant Arg-46.1 Publication
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_002208 | 46 | C → R in 45% of the population. 1 PublicationCorresponds to variant dbSNP:rs1799841Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59964 Genomic DNA Translation: CAA42590.1 X70377 mRNA Translation: CAA49838.1 AL591074 Genomic DNA No translation available. BC062678 mRNA Translation: AAH62678.1 BC069514 mRNA Translation: AAH69514.1 |
CCDSi | CCDS13162.1 |
PIRi | A47142 |
RefSeqi | NP_001891.2, NM_001900.4 |
Genome annotation databases
Ensembli | ENST00000304710; ENSP00000307132; ENSG00000170367 |
GeneIDi | 1473 |
KEGGi | hsa:1473 |
UCSCi | uc002wtr.2, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59964 Genomic DNA Translation: CAA42590.1 X70377 mRNA Translation: CAA49838.1 AL591074 Genomic DNA No translation available. BC062678 mRNA Translation: AAH62678.1 BC069514 mRNA Translation: AAH69514.1 |
CCDSi | CCDS13162.1 |
PIRi | A47142 |
RefSeqi | NP_001891.2, NM_001900.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RN7 | X-ray | 2.50 | A | 21-142 | [»] | |
1ROA | X-ray | 1.80 | A | 21-142 | [»] | |
SMRi | P28325 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107855, 24 interactors |
IntActi | P28325, 7 interactors |
STRINGi | 9606.ENSP00000307132 |
Protein family/group databases
MEROPSi | I25.005 |
Polymorphism and mutation databases
BioMutai | CST5 |
Proteomic databases
jPOSTi | P28325 |
MassIVEi | P28325 |
PaxDbi | P28325 |
PeptideAtlasi | P28325 |
PRIDEi | P28325 |
ProteomicsDBi | 54464 |
Protocols and materials databases
Antibodypediai | 24931, 135 antibodies |
DNASUi | 1473 |
Genome annotation databases
Ensembli | ENST00000304710; ENSP00000307132; ENSG00000170367 |
GeneIDi | 1473 |
KEGGi | hsa:1473 |
UCSCi | uc002wtr.2, human |
Organism-specific databases
CTDi | 1473 |
DisGeNETi | 1473 |
EuPathDBi | HostDB:ENSG00000170367.4 |
GeneCardsi | CST5 |
HGNCi | HGNC:2477, CST5 |
HPAi | ENSG00000170367, Tissue enriched (salivary) |
MIMi | 123858, gene |
neXtProti | NX_P28325 |
OpenTargetsi | ENSG00000170367 |
PharmGKBi | PA26978 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SC50, Eukaryota |
GeneTreei | ENSGT00940000164560 |
HOGENOMi | CLU_118168_0_1_1 |
InParanoidi | P28325 |
OMAi | FTIREYN |
OrthoDBi | 1565344at2759 |
PhylomeDBi | P28325 |
Enzyme and pathway databases
PathwayCommonsi | P28325 |
Miscellaneous databases
BioGRID-ORCSi | 1473, 3 hits in 844 CRISPR screens |
ChiTaRSi | CST5, human |
EvolutionaryTracei | P28325 |
GeneWikii | CST5 |
GenomeRNAii | 1473 |
Pharosi | P28325, Tbio |
PROi | PR:P28325 |
RNActi | P28325, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170367, Expressed in saliva-secreting gland and 59 other tissues |
Genevisiblei | P28325, HS |
Family and domain databases
CDDi | cd00042, CY, 1 hit |
InterProi | View protein in InterPro IPR000010, Cystatin_dom IPR018073, Prot_inh_cystat_CS |
Pfami | View protein in Pfam PF00031, Cystatin, 1 hit |
SMARTi | View protein in SMART SM00043, CY, 1 hit |
PROSITEi | View protein in PROSITE PS00287, CYSTATIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYTD_HUMAN | |
Accessioni | P28325Primary (citable) accession number: P28325 Secondary accession number(s): Q5JRF5, Q9UCA0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations