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Protein

ETS translocation variant 4

Gene

Etv4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. May play a regulatory role during embryogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi342 – 422ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

  • DNA binding Source: MGI
  • DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-5687128 MAPK6/MAPK4 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
ETS translocation variant 4
Alternative name(s):
Polyomavirus enhancer activator 3
Short name:
Protein PEA3
Gene namesi
Name:Etv4
Synonyms:Pea-3, Pea3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:99423 Etv4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041171 – 485ETS translocation variant 4Add BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki95Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei100PhosphoserineBy similarity1
Cross-linki138Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei139PhosphoserineBy similarity1
Modified residuei148PhosphoserineBy similarity1
Modified residuei215PhosphoserineBy similarity1
Cross-linki227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki261Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki323Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylated.
Sumoylated; enhanced upon ERK/MAP kinase pathway activation it positively regulates the transcriptional activator capacity. Sumoylation at Lys-95 probably requires phosphorylation at Ser-100. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2 (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP28322
PRIDEiP28322

PTM databases

iPTMnetiP28322
PhosphoSitePlusiP28322

Expressioni

Tissue specificityi

Epididymis and brain.

Gene expression databases

BgeeiENSMUSG00000017724 Expressed in 201 organ(s), highest expression level in thymus
ExpressionAtlasiP28322 baseline and differential
GenevisibleiP28322 MM

Interactioni

Protein-protein interaction databases

BioGridi202104, 2 interactors
DIPiDIP-60466N
IntActiP28322, 1 interactor
STRINGi10090.ENSMUSP00000129261

Structurei

3D structure databases

ProteinModelPortaliP28322
SMRiP28322
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 75Asp/Glu-rich (acidic)Add BLAST27
Compositional biasi147 – 245Gln-richAdd BLAST99

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3806 Eukaryota
ENOG410Z0ZF LUCA
GeneTreeiENSGT00760000118907
HOGENOMiHOG000230986
HOVERGENiHBG000231
InParanoidiP28322
KOiK15592

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR006715 ETS_PEA3_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PF04621 ETS_PEA3_N, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P28322-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MERRMKGGYL DQRVPYTFCS KSPGNGSLGE ALMVPQGKLM DPGSLPPSDS
60 70 80 90 100
EDLFQDLSHF QETWLAEAQV PDSDEQFVPD FHSENSFHSP TTRIKKEPQS
110 120 130 140 150
PRTDPALSCS RKPPLPYHHG EQCLYSSAYD SPRQIAIKSP APGAPGQSPL
160 170 180 190 200
QPFSRAEQQQ SLLRASSSSQ SHPGHGYLGE HSSVFQQPVD MCHSFTSPQG
210 220 230 240 250
GGREPLPAPY QHQLSEPCPP YPQQNFKQEY HDPLYEQAGQ PASSQGGVSG
260 270 280 290 300
HRYPGAGVVI KQERTDFAYD SDVPGCASMY LHPEGFSGPS PGDGVMGYGY
310 320 330 340 350
EKSLRPFPDD VCIVPEKFEG DIKQEGIGAF REGPPYQRRG ALQLWQFLVA
360 370 380 390 400
LLDDPTNAHF IAWTGRGMEF KLIEPEEVAR LWGIQKNRPA MNYDKLSRSL
410 420 430 440 450
RYYYEKGIMQ KVAGERYVYK FVCEPEALFS LAFPDNQRPA LKAEFDRPVS
460 470 480
EEDTVPLSHL DESPAYLPEL TGPAPPFGHR GGYSY
Length:485
Mass (Da):54,008
Last modified:September 18, 2013 - v2
Checksum:i3A5FF4EFD7E828EF
GO
Isoform 2 (identifier: P28322-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTKSSNHNCL...GPVAGGSGRM
     86-86: S → LA
     127-132: Missing.

Note: No experimental confirmation available.
Show »
Length:555
Mass (Da):60,847
Checksum:i778AB1AF19B202BF
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A5C3A2A5C3_MOUSE
ETS translocation variant 4
Etv4
486Annotation score:
A2A5C2A2A5C2_MOUSE
ETS translocation variant 4
Etv4
480Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316E → K in CAA44872 (PubMed:1547944).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0479431M → MTKSSNHNCLLRPENKPGLW GPGAQAASLRPSPATLVVSS PGHAEHPPAAPAQTPGPQVS ASARGPGPVAGGSGRM in isoform 2. 1 Publication1
Alternative sequenceiVSP_04794486S → LA in isoform 2. 1 Publication1
Alternative sequenceiVSP_047945127 – 132Missing in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63190 mRNA Translation: CAA44872.1
AK144590 mRNA Translation: BAE25952.1
AL591436 Genomic DNA No translation available.
CCDSiCCDS48937.1 [P28322-1]
PIRiS24061
RefSeqiNP_032841.2, NM_008815.3 [P28322-1]
UniGeneiMm.5025

Genome annotation databases

EnsembliENSMUST00000164750; ENSMUSP00000129261; ENSMUSG00000017724 [P28322-1]
GeneIDi18612
KEGGimmu:18612
UCSCiuc007lpt.1 mouse [P28322-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63190 mRNA Translation: CAA44872.1
AK144590 mRNA Translation: BAE25952.1
AL591436 Genomic DNA No translation available.
CCDSiCCDS48937.1 [P28322-1]
PIRiS24061
RefSeqiNP_032841.2, NM_008815.3 [P28322-1]
UniGeneiMm.5025

3D structure databases

ProteinModelPortaliP28322
SMRiP28322
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202104, 2 interactors
DIPiDIP-60466N
IntActiP28322, 1 interactor
STRINGi10090.ENSMUSP00000129261

PTM databases

iPTMnetiP28322
PhosphoSitePlusiP28322

Proteomic databases

PaxDbiP28322
PRIDEiP28322

Protocols and materials databases

DNASUi18612
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000164750; ENSMUSP00000129261; ENSMUSG00000017724 [P28322-1]
GeneIDi18612
KEGGimmu:18612
UCSCiuc007lpt.1 mouse [P28322-1]

Organism-specific databases

CTDi2118
MGIiMGI:99423 Etv4

Phylogenomic databases

eggNOGiKOG3806 Eukaryota
ENOG410Z0ZF LUCA
GeneTreeiENSGT00760000118907
HOGENOMiHOG000230986
HOVERGENiHBG000231
InParanoidiP28322
KOiK15592

Enzyme and pathway databases

ReactomeiR-MMU-5687128 MAPK6/MAPK4 signaling

Miscellaneous databases

ChiTaRSiEtv4 mouse
PROiPR:P28322
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017724 Expressed in 201 organ(s), highest expression level in thymus
ExpressionAtlasiP28322 baseline and differential
GenevisibleiP28322 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR006715 ETS_PEA3_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PF04621 ETS_PEA3_N, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiETV4_MOUSE
AccessioniPrimary (citable) accession number: P28322
Secondary accession number(s): Q3UMZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: September 18, 2013
Last modified: November 7, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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