UniProtKB - P28301 (LYOX_MOUSE)
Protein
Protein-lysine 6-oxidase
Gene
Lox
Organism
Mus musculus (Mouse)
Status
Functioni
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (PubMed:27432961).1 Publication
Catalytic activityi
- EC:1.4.3.131 Publication
Cofactori
Protein has several cofactor binding sites:- Cu cationBy similarity
- lysine tyrosylquinone residueBy similarityNote: Contains 1 lysine tyrosylquinone.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 286 | CopperSequence analysis | 1 | |
Metal bindingi | 288 | CopperSequence analysis | 1 | |
Metal bindingi | 290 | CopperSequence analysis | 1 |
GO - Molecular functioni
- collagen binding Source: UniProtKB
- copper ion binding Source: MGI
- protein-lysine 6-oxidase activity Source: UniProtKB
GO - Biological processi
- aorta development Source: MGI
- ascending aorta development Source: MGI
- blood vessel development Source: MGI
- blood vessel morphogenesis Source: UniProtKB
- bone mineralization Source: MGI
- cell chemotaxis Source: MGI
- cellular response to chemokine Source: MGI
- cellular response to organic substance Source: MGI
- collagen fibril organization Source: MGI
- connective tissue development Source: MGI
- descending aorta development Source: MGI
- DNA biosynthetic process Source: MGI
- elastic fiber assembly Source: MGI
- heart development Source: MGI
- lung development Source: MGI
- muscle cell cellular homeostasis Source: MGI
- muscle fiber development Source: MGI
- osteoblast differentiation Source: MGI
- peptidyl-lysine oxidation Source: UniProtKB
- platelet-derived growth factor receptor-beta signaling pathway Source: MGI
- protein kinase B signaling Source: MGI
- protein oxidation Source: MGI
- regulation of apoptotic process Source: MGI
- regulation of bone development Source: MGI
- regulation of gene expression Source: MGI
- regulation of megakaryocyte differentiation Source: MGI
- regulation of platelet-derived growth factor receptor-beta signaling pathway Source: MGI
- regulation of protein phosphorylation Source: MGI
- regulation of receptor binding Source: MGI
- regulation of striated muscle tissue development Source: MGI
- regulation of transforming growth factor beta receptor signaling pathway Source: MGI
- response to drug Source: Ensembl
- response to hormone Source: MGI
- response to steroid hormone Source: MGI
- wound healing Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Copper, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1566948, Elastic fibre formation R-MMU-2243919, Crosslinking of collagen fibrils |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-lysine 6-oxidase (EC:1.4.3.131 Publication)Alternative name(s): Lysyl oxidase Ras excision protein Cleaved into the following 2 chains: Protein-lysine 6-oxidase, long formBy similarity Protein-lysine 6-oxidase, short formBy similarity |
Gene namesi | Name:Lox Synonyms:Rrg |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96817, Lox |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
- extracellular space
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular matrix Source: MGI
- extracellular region Source: UniProtKB
- extracellular space Source: BHF-UCL
Nucleus
- nucleus Source: MGI
Other locations
- collagen trimer Source: MGI
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 292 | M → R: Homozygous mice display tortuous vessels aneurysm, loss of oxidase activity and die perinatally of aortic aneurysm and dissection. Heterozygous mice display thicker arterial wall with discontinuous elastic lamellae. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | By similarityAdd BLAST | 21 | |
PropeptideiPRO_0000018522 | 22 – 162 | Removed by BMP1By similarityAdd BLAST | 141 | |
ChainiPRO_0000018523 | 163 – 411 | Protein-lysine 6-oxidase, long formBy similarityAdd BLAST | 249 | |
ChainiPRO_0000447886 | 213 – 411 | Protein-lysine 6-oxidase, short formBy similarityAdd BLAST | 199 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 91 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 138 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 181 | SulfotyrosineBy similarity | 1 | |
Disulfide bondi | 232 ↔ 238 | By similarity | ||
Disulfide bondi | 285 ↔ 334 | By similarity | ||
Cross-linki | 314 ↔ 349 | Lysine tyrosylquinone (Lys-Tyr)By similarity | ||
Disulfide bondi | 318 ↔ 324 | By similarity | ||
Disulfide bondi | 345 ↔ 355 | By similarity | ||
Modified residuei | 349 | 2',4',5'-topaquinoneBy similarity | 1 | |
Disulfide bondi | 392 ↔ 406 | By similarity |
Post-translational modificationi
The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.By similarity
Proteolytically cleaved by BMP1 which removes the propeptide (PubMed:20181949). Also proteolytically cleaved by ADAMTS2 and ADAMTS14, but not by ADAMTS3, at an additional cleavage site downstream of the BMP1 cleavage site (By similarity). The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin (PubMed:16251195). Cleavage by BMP1 to remove the propeptide does not increase enzymatic activity but increases binding to collagen (By similarity). Cleavage by ADAMTS2 produces a form with reduced collagen-binding activity (By similarity).By similarity2 Publications
Sulfated at Tyr-181 and also at either Tyr-177 or Tyr-178 which enhances binding to collagen.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 212 – 213 | Cleavage; by ADAMTS2 and ADAMTS14By similarity | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, LTQ, Sulfation, TPQProteomic databases
PaxDbi | P28301 |
PeptideAtlasi | P28301 |
PRIDEi | P28301 |
PTM databases
GlyGeni | P28301, 2 sites |
iPTMneti | P28301 |
PhosphoSitePlusi | P28301 |
Expressioni
Tissue specificityi
Expressed in aorta (at protein level). Expressed in embryonic, juvenil and adult aorta.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000024529, Expressed in stroma of bone marrow and 222 other tissues |
ExpressionAtlasi | P28301, baseline and differential |
Genevisiblei | P28301, MM |
Interactioni
Subunit structurei
Interacts with MFAP4.
By similarityBinary interactionsi
Hide detailsP28301
With | #Exp. | IntAct |
---|---|---|
Adamtsl2 [Q7TSK7] | 3 | EBI-642911,EBI-25406979 |
Hspa1b [P17879] | 2 | EBI-642911,EBI-397360 |
ADAMTS10 [Q9H324] from Homo sapiens. | 3 | EBI-642911,EBI-7096115 |
ADAMTSL4 [Q6UY14] from Homo sapiens. | 2 | EBI-642911,EBI-742002 |
HSPA1B [P08107] From a different organism. | 6 | EBI-642911,EBI-629985 |
RAF1 [P04049] from Homo sapiens. | 2 | EBI-642911,EBI-365996 |
Protein-protein interaction databases
BioGRIDi | 201191, 5 interactors |
IntActi | P28301, 16 interactors |
MINTi | P28301 |
STRINGi | 10090.ENSMUSP00000129247 |
Miscellaneous databases
RNActi | P28301, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 207 – 411 | Lysyl-oxidase likeAdd BLAST | 205 |
Sequence similaritiesi
Belongs to the lysyl oxidase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QWQR, Eukaryota |
GeneTreei | ENSGT00940000154779 |
HOGENOMi | CLU_002555_2_1_1 |
InParanoidi | P28301 |
OMAi | IVRCDVR |
OrthoDBi | 815466at2759 |
PhylomeDBi | P28301 |
TreeFami | TF326061 |
Family and domain databases
InterProi | View protein in InterPro IPR001695, Lysyl_oxidase IPR019828, Lysyl_oxidase_CS |
Pfami | View protein in Pfam PF01186, Lysyl_oxidase, 1 hit |
PRINTSi | PR00074, LYSYLOXIDASE |
PROSITEi | View protein in PROSITE PS00926, LYSYL_OXIDASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P28301-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRFAWAVLLL GPLQLCPLLR CAPQTPREPP AAPGAWRQTI QWENNGQVFS
60 70 80 90 100
LLSLGAQYQP QRRRDPSATA RRPDGDAASQ PRTPILLLRD NRTASTRART
110 120 130 140 150
PSPSGVAAGR PRPAARHWFQ AGFSPSGARD GASRRAANRT ASPQPPQLSN
160 170 180 190 200
LRPPSHIDRM VGDDPYNPYK YSDDNPYYNY YDTYERPRPG SRNRPGYGTG
210 220 230 240 250
YFQYGLPDLV PDPYYIQAST YVQKMSMYNL RCAAEENCLA SSAYRADVRD
260 270 280 290 300
YDHRVLLRFP QRVKNQGTSD FLPSRPRYSW EWHSCHQHYH SMDEFSHYDL
310 320 330 340 350
LDANTQRRVA EGHKASFCLE DTSCDYGYHR RFACTAHTQG LSPGCYDTYA
360 370 380 390 400
ADIDCQWIDI TDVQPGNYIL KVSVNPSYLV PESDYTNNVV RCDIRYTGHH
410
AYASGCTISP Y
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 333 | A → G in AAA99899 (PubMed:8100214).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65142 mRNA Translation: AAA19032.1 M65143 mRNA Translation: AAA20185.1 L04262, L04263, L04264 Genomic DNA Translation: AAA99899.1 BC018439 mRNA Translation: AAH18439.1 |
CCDSi | CCDS50289.1 |
PIRi | A47005, OXMSL |
RefSeqi | NP_001273110.1, NM_001286181.1 NP_001273111.1, NM_001286182.1 NP_034858.2, NM_010728.3 |
Genome annotation databases
Ensembli | ENSMUST00000025409; ENSMUSP00000025409; ENSMUSG00000024529 ENSMUST00000171470; ENSMUSP00000129247; ENSMUSG00000024529 |
GeneIDi | 16948 |
KEGGi | mmu:16948 |
UCSCi | uc008exe.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65142 mRNA Translation: AAA19032.1 M65143 mRNA Translation: AAA20185.1 L04262, L04263, L04264 Genomic DNA Translation: AAA99899.1 BC018439 mRNA Translation: AAH18439.1 |
CCDSi | CCDS50289.1 |
PIRi | A47005, OXMSL |
RefSeqi | NP_001273110.1, NM_001286181.1 NP_001273111.1, NM_001286182.1 NP_034858.2, NM_010728.3 |
3D structure databases
SMRi | P28301 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201191, 5 interactors |
IntActi | P28301, 16 interactors |
MINTi | P28301 |
STRINGi | 10090.ENSMUSP00000129247 |
PTM databases
GlyGeni | P28301, 2 sites |
iPTMneti | P28301 |
PhosphoSitePlusi | P28301 |
Proteomic databases
PaxDbi | P28301 |
PeptideAtlasi | P28301 |
PRIDEi | P28301 |
Protocols and materials databases
Antibodypediai | 25606, 795 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025409; ENSMUSP00000025409; ENSMUSG00000024529 ENSMUST00000171470; ENSMUSP00000129247; ENSMUSG00000024529 |
GeneIDi | 16948 |
KEGGi | mmu:16948 |
UCSCi | uc008exe.2, mouse |
Organism-specific databases
CTDi | 4015 |
MGIi | MGI:96817, Lox |
Phylogenomic databases
eggNOGi | ENOG502QWQR, Eukaryota |
GeneTreei | ENSGT00940000154779 |
HOGENOMi | CLU_002555_2_1_1 |
InParanoidi | P28301 |
OMAi | IVRCDVR |
OrthoDBi | 815466at2759 |
PhylomeDBi | P28301 |
TreeFami | TF326061 |
Enzyme and pathway databases
Reactomei | R-MMU-1566948, Elastic fibre formation R-MMU-2243919, Crosslinking of collagen fibrils |
Miscellaneous databases
BioGRID-ORCSi | 16948, 1 hit in 17 CRISPR screens |
ChiTaRSi | Lox, mouse |
PROi | PR:P28301 |
RNActi | P28301, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024529, Expressed in stroma of bone marrow and 222 other tissues |
ExpressionAtlasi | P28301, baseline and differential |
Genevisiblei | P28301, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001695, Lysyl_oxidase IPR019828, Lysyl_oxidase_CS |
Pfami | View protein in Pfam PF01186, Lysyl_oxidase, 1 hit |
PRINTSi | PR00074, LYSYLOXIDASE |
PROSITEi | View protein in PROSITE PS00926, LYSYL_OXIDASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LYOX_MOUSE | |
Accessioni | P28301Primary (citable) accession number: P28301 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | February 10, 2021 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families