UniProtKB - P28299 (ACEA_NEUCR)
Protein
Isocitrate lyase
Gene
acu-3
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Functioni
Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity
Catalytic activityi
Cofactori
Mg2+By similarity
: glyoxylate cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Isocitrate lyase (acu-3)
- Malate synthase, glyoxysomal (acu-9)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 177 | MagnesiumBy similarity | 1 | |
Active sitei | 215 | Proton acceptorBy similarity | 1 | |
Binding sitei | 252 | SubstrateBy similarity | 1 | |
Binding sitei | 468 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- isocitrate lyase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- methylisocitrate lyase activity Source: UniProtKB-EC
GO - Biological processi
- glyoxylate cycle Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
Names & Taxonomyi
Protein namesi | Recommended name: Isocitrate lyase1 Publication (EC:4.1.3.1By similarity)Short name: ICLCurated Short name: IsocitraseCurated Short name: IsocitrataseCurated Alternative name(s): Threo-D(S)-isocitrate glyoxylate-lyaseCurated |
Gene namesi | Name:acu-31 Publication ORF Names:2E4.10, NCU04230 |
Organismi | Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
Taxonomic identifieri | 367110 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:NCU04230 |
Subcellular locationi
Peroxisome
- Glyoxysome 1 Publication
Peroxisome
- glyoxysome Source: UniProtKB-SubCell
Keywords - Cellular componenti
Glyoxysome, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068794 | 1 – 548 | Isocitrate lyaseAdd BLAST | 548 |
Expressioni
Inductioni
By acetate.1 Publication
Interactioni
Subunit structurei
Homotetramer.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 106 – 108 | Substrate bindingBy similarity | 3 | |
Regioni | 216 – 217 | Substrate bindingBy similarity | 2 | |
Regioni | 434 – 438 | Substrate bindingBy similarity | 5 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_019214_2_2_1 |
InParanoidi | P28299 |
OMAi | LEKDWAE |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 1 hit |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P28299-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAANNMVNPA VDPALEDELF AKEVEEVKKW WSDSRWRQTK RPFTAEQIVS
60 70 80 90 100
KRGNLKIEYA SNAQAKKLWK ILEDRFAKRD ASYTYGCLEP TMVTQMAKYL
110 120 130 140 150
DTVYVSGWQS SSTASSSDEP GPDLADYPYT TVPNKVGHLF MAQLFHDRKQ
160 170 180 190 200
RQERLSVPKD QREKLANIDY LRPIVADADT GHGGLTAVMK LTKLFIEKGA
210 220 230 240 250
AGIHIEDQAP GTKKCGHMAG KVLVPIQEHI NRLVAIRAQA DIMGSDLLCI
260 270 280 290 300
ARTDAEAATL ITTTIDPRDH AFILGCTNPD LEPLADLMMK AEAEGKTGAQ
310 320 330 340 350
LQAIEDDWLA KADLKRFDEA VLDVIAKGKF SNAKDLAAKY QAAVKGKQIS
360 370 380 390 400
NREARAIARQ LLGQEIFFDW ESPRTREGYY RLKGGCDCSI NRAISYAPYC
410 420 430 440 450
DAIWMESKLP DYAQAEEFAK GVHAVWPEQK LAYNLSPSFN WKTAMGRDDQ
460 470 480 490 500
ETYIRRLAKL GYCWQFITLA GLHTTALISD QFAKAYSKIG MRAYGELVQE
510 520 530 540
PEIDNGVDVV KHQKWSGATY VDELQKMVTG GVSSTAAMGK GVTEDQFH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 132 | V → C in CAA44573 (PubMed:1531185).Curated | 1 | |
Sequence conflicti | 286 | D → H in CAA44573 (PubMed:1531185).Curated | 1 | |
Sequence conflicti | 422 – 424 | VHA → PR in CAA44573 (PubMed:1531185).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62697 Genomic DNA Translation: CAA44573.1 AL451022 Genomic DNA Translation: CAC18302.1 CM002240 Genomic DNA Translation: EAA31618.1 |
PIRi | S26858 |
RefSeqi | XP_960854.1, XM_955761.3 |
Genome annotation databases
EnsemblFungii | EAA31618; EAA31618; NCU04230 |
GeneIDi | 3877001 |
KEGGi | ncr:NCU04230 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62697 Genomic DNA Translation: CAA44573.1 AL451022 Genomic DNA Translation: CAC18302.1 CM002240 Genomic DNA Translation: EAA31618.1 |
PIRi | S26858 |
RefSeqi | XP_960854.1, XM_955761.3 |
3D structure databases
SMRi | P28299 |
ModBasei | Search... |
Genome annotation databases
EnsemblFungii | EAA31618; EAA31618; NCU04230 |
GeneIDi | 3877001 |
KEGGi | ncr:NCU04230 |
Organism-specific databases
VEuPathDBi | FungiDB:NCU04230 |
Phylogenomic databases
HOGENOMi | CLU_019214_2_2_1 |
InParanoidi | P28299 |
OMAi | LEKDWAE |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 1 hit |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACEA_NEUCR | |
Accessioni | P28299Primary (citable) accession number: P28299 Secondary accession number(s): Q7RVC2, Q9HEI3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families