UniProtKB - P28298 (ACEA_EMENI)
Protein
Isocitrate lyase
Gene
acuD
Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Functioni
Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity
Catalytic activityi
Cofactori
Mg2+By similarity
: glyoxylate cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Isocitrate lyase (acuD)
- Malate synthase, glyoxysomal (acuE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 168 | MagnesiumBy similarity | 1 | |
Active sitei | 206 | Proton acceptor1 Publication | 1 | |
Binding sitei | 243 | SubstrateBy similarity | 1 | |
Binding sitei | 457 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- isocitrate lyase activity Source: AspGD
- metal ion binding Source: UniProtKB-KW
- methylisocitrate lyase activity Source: UniProtKB-EC
GO - Biological processi
- acetate catabolic process Source: AspGD
- carbon utilization Source: AspGD
- fatty acid catabolic process Source: AspGD
- glyoxylate cycle Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
Names & Taxonomyi
Protein namesi | Recommended name: Isocitrate lyase1 Publication (EC:4.1.3.1By similarity)Short name: ICLCurated Short name: IsocitraseCurated Short name: IsocitrataseCurated Alternative name(s): Threo-D(S)-isocitrate glyoxylate-lyaseCurated |
Gene namesi | Name:acuD1 Publication ORF Names:AN5634 |
Organismi | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
Taxonomic identifieri | 227321 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Nidulantes › |
Proteomesi |
|
Subcellular locationi
Peroxisome
- Glyoxysome By similarity
Peroxisome
- glyoxysome Source: UniProtKB-SubCell
- peroxisomal matrix Source: AspGD
- peroxisome Source: AspGD
Keywords - Cellular componenti
Glyoxysome, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068790 | 1 – 538 | Isocitrate lyaseAdd BLAST | 538 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
STRINGi | 162425.CADANIAP00003436 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28298 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28298 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 97 – 99 | Substrate bindingBy similarity | 3 | |
Regioni | 207 – 208 | Substrate bindingBy similarity | 2 | |
Regioni | 423 – 427 | Substrate bindingBy similarity | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1260, Eukaryota |
HOGENOMi | CLU_019214_2_2_1 |
InParanoidi | P28298 |
OMAi | LEKDWAE |
OrthoDBi | 905115at2759 |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 1 hit |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P28298-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSYIEEEDQR YWDEVAAVKN WWKDSRWRYT KRPFTAEQIV AKRGNLKIEY
60 70 80 90 100
PSNVQAKKLW GILERNFKNK EASFTYGCLD PTMVTQMAKY LDTVYVSGWQ
110 120 130 140 150
SSSTASSTDE PSPDLADYPM NTVPNKVNHL WMAQLFHDRK QREERMTTPK
160 170 180 190 200
DQRHKVANVD YLRPIIADAD TGHGGLTAVM KLTKLFVERG AAGIHIEDQA
210 220 230 240 250
PGTKKCGHMA GKVLVPISEH INRLVAIRAQ ADIMGTDLLA IARTDSEAAT
260 270 280 290 300
LITSTIDHRD HPFIIGSTNP DIQPLNDLMV MAEQAGKNGA ELQAIEDEWL
310 320 330 340 350
AKAGLKLFND AVVDAINNSP LPNKKAAIEK YLTQSKGKSN LEARAIAKEI
360 370 380 390 400
AGTDIYFDWE APRTREGYYR YQGGTQCAIN RAVAYAPFAD LIWMESKLPD
410 420 430 440 450
YKQAKEFADG VHAVWPEQKL AYNLSPSFNW KKAMPRDEQE TYIKRLGALG
460 470 480 490 500
YAWQFITLAG LHTTALISDT FAKAYAKQGM RAYGELVQEP EMANGVDVVT
510 520 530
HQKWSGANYV DNMLKMITGG VSSTAAMGKG VTEDQFKS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 17 | Missing in CAA44572 (PubMed:1531185).Curated | 1 | |
Sequence conflicti | 68 | Missing in CAA44572 (PubMed:1531185).Curated | 1 | |
Sequence conflicti | 157 | A → T in CAA44572 (PubMed:1531185).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62696 Genomic DNA Translation: CAA44572.1 AACD01000098 Genomic DNA Translation: EAA62727.1 BN001305 Genomic DNA Translation: CBF81510.1 |
PIRi | S26857 |
RefSeqi | XP_663238.1, XM_658146.1 |
Genome annotation databases
EnsemblFungii | CBF81510; CBF81510; ANIA_05634 EAA62727; EAA62727; AN5634.2 |
GeneIDi | 2871925 |
KEGGi | ani:AN5634.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62696 Genomic DNA Translation: CAA44572.1 AACD01000098 Genomic DNA Translation: EAA62727.1 BN001305 Genomic DNA Translation: CBF81510.1 |
PIRi | S26857 |
RefSeqi | XP_663238.1, XM_658146.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DQU | X-ray | 2.80 | A | 1-538 | [»] | |
SMRi | P28298 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 162425.CADANIAP00003436 |
Genome annotation databases
EnsemblFungii | CBF81510; CBF81510; ANIA_05634 EAA62727; EAA62727; AN5634.2 |
GeneIDi | 2871925 |
KEGGi | ani:AN5634.2 |
Phylogenomic databases
eggNOGi | KOG1260, Eukaryota |
HOGENOMi | CLU_019214_2_2_1 |
InParanoidi | P28298 |
OMAi | LEKDWAE |
OrthoDBi | 905115at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
Miscellaneous databases
EvolutionaryTracei | P28298 |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 1 hit |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACEA_EMENI | |
Accessioni | P28298Primary (citable) accession number: P28298 Secondary accession number(s): C8VFW4, Q5B1E6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | May 1, 2007 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families