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UniProtKB - P28296 (ORYZ_ASPFU)
Protein
Alkaline protease 1
Gene
alp1
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Functioni
Secreted alkaline protease that allows assimilation of proteinaceous substrates. Acts as a significant virulence factor in invasive aspergillosis. Involved in immune evasion from the human and mice complement systems during infection. Efficiently cleaves important components of the complement cascade such as such as C3, C4, C5, and C1q, as well as IgG, which leads to down-regulation of complement activation at the hyphal surface.
3 PublicationsCatalytic activityi
- Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides. EC:3.4.21.63
pH dependencei
Optimum pH is 7-8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 162 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 193 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 349 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- fibrinogen binding Source: AspGD
- IgE binding Source: UniProtKB
- peptidase activity Source: UniProtKB
- serine-type endopeptidase activity Source: GO_Central
- serine-type peptidase activity Source: UniProtKB
GO - Biological processi
- complement activation Source: AspGD
- elastin catabolic process Source: AspGD
- evasion of host immune response via regulation of host complement system Source: UniProtKB
- protein catabolic process Source: AspGD
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Virulence |
Protein family/group databases
MEROPSi | S08.053 |
Names & Taxonomyi
Protein namesi | Recommended name: Alkaline protease 1 (EC:3.4.21.63)Short name: ALP Alternative name(s): Aspergillopeptidase B Aspergillus proteinase B Elastase Elastinolytic serine proteinase Oryzin Allergen: Asp f 13 |
Gene namesi | Name:alp1 Synonyms:alk1, alp ORF Names:AFUA_4G11800 |
Organismi | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
Taxonomic identifieri | 330879 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:Afu4g11800 |
Subcellular locationi
Extracellular region or secreted
- Secreted 4 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPathology & Biotechi
Allergenic propertiesi
Causes an allergic reaction in human. Binds to IgE via three immunodominant epitopes localized at the C-terminal part.
Keywords - Diseasei
AllergenProtein family/group databases
Allergomei | 3111, Asp f 13.0101 66, Asp f 13 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | By similarityAdd BLAST | 21 | |
PropeptideiPRO_0000027082 | 22 – 101 | 1 PublicationAdd BLAST | 80 | |
ChainiPRO_0000027083 | 102 – 403 | Alkaline protease 1Add BLAST | 302 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 253 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 307 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 367 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, ZymogenExpressioni
Inductioni
Expression is controlled by the prtT transcription factor.2 Publications
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 120 | Inhibitor I9Sequence analysisAdd BLAST | 85 | |
Domaini | 130 – 403 | Peptidase S8PROSITE-ProRule annotationAdd BLAST | 274 |
Sequence similaritiesi
Belongs to the peptidase S8 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1153, Eukaryota |
HOGENOMi | CLU_011263_1_4_1 |
InParanoidi | P28296 |
OMAi | GEGVWAY |
OrthoDBi | 921536at2759 |
Family and domain databases
CDDi | cd04077, Peptidases_S8_PCSK9_ProteinaseK_like, 1 hit |
Gene3Di | 3.30.70.80, 1 hit 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR034193, PCSK9_ProteinaseK-like IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR023827, Peptidase_S8_Asp-AS IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel IPR010259, S8pro/Inhibitor_I9 IPR037045, S8pro/Inhibitor_I9_sf |
Pfami | View protein in Pfam PF05922, Inhibitor_I9, 1 hit PF00082, Peptidase_S8, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51892, SUBTILASE, 1 hit PS00136, SUBTILASE_ASP, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P28296-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSIKRTLLL LGAVLPAVFG APVQETRRAA QKIPGKYIVT FKPGTDTATI
60 70 80 90 100
ESHTLWATDL HKRNLERRDT TSGEPPVGIE KSYKIKDFAA YAGSFDDATI
110 120 130 140 150
EEIRKSADVA HVEEDQIWYL DALTTQKGAP WGLGSISHKG QASTDYIYDT
160 170 180 190 200
SAGAGTYAYV VDSGINVNHV EFESRASLAY NAAGGSHVDS IGHGTHVAGT
210 220 230 240 250
IGGKTYGVAK KTNLLSVKVF QGESSSTSII LDGFNWAVND IVSKGRTKKA
260 270 280 290 300
AINMSLGGGY SYAFNNAVEN AFDEGVLSVV AAGNENSDAS NTSPASAPNA
310 320 330 340 350
LTVAAINKSN ARASFSNYGS VVDIFAPGQD ILSAWIGSTT ATNTISGTSM
360 370 380 390 400
ATPHIVGLSV YLMGLENLSG PAAVTARIKE LATNGVVTNV KGSPNKLAYN
GNA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 106 – 107 | SA → RG in CAA77666 (PubMed:1601287).Curated | 2 | |
Sequence conflicti | 295 | A → R in AAB07672 (PubMed:8500876).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11580 Genomic DNA Translation: CAA77666.1 M99420 Genomic DNA Translation: AAB07672.1 AAHF01000005 Genomic DNA Translation: EAL89613.1 |
PIRi | S22184 |
RefSeqi | XP_751651.1, XM_746558.1 |
Genome annotation databases
EnsemblFungii | EAL89613; EAL89613; AFUA_4G11800 |
GeneIDi | 3509271 |
KEGGi | afm:AFUA_4G11800 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11580 Genomic DNA Translation: CAA77666.1 M99420 Genomic DNA Translation: AAB07672.1 AAHF01000005 Genomic DNA Translation: EAL89613.1 |
PIRi | S22184 |
RefSeqi | XP_751651.1, XM_746558.1 |
3D structure databases
SMRi | P28296 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 746128.CADAFUBP00006700 |
Protein family/group databases
Allergomei | 3111, Asp f 13.0101 66, Asp f 13 |
MEROPSi | S08.053 |
Genome annotation databases
EnsemblFungii | EAL89613; EAL89613; AFUA_4G11800 |
GeneIDi | 3509271 |
KEGGi | afm:AFUA_4G11800 |
Organism-specific databases
VEuPathDBi | FungiDB:Afu4g11800 |
Phylogenomic databases
eggNOGi | KOG1153, Eukaryota |
HOGENOMi | CLU_011263_1_4_1 |
InParanoidi | P28296 |
OMAi | GEGVWAY |
OrthoDBi | 921536at2759 |
Family and domain databases
CDDi | cd04077, Peptidases_S8_PCSK9_ProteinaseK_like, 1 hit |
Gene3Di | 3.30.70.80, 1 hit 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR034193, PCSK9_ProteinaseK-like IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR023827, Peptidase_S8_Asp-AS IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel IPR010259, S8pro/Inhibitor_I9 IPR037045, S8pro/Inhibitor_I9_sf |
Pfami | View protein in Pfam PF05922, Inhibitor_I9, 1 hit PF00082, Peptidase_S8, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51892, SUBTILASE, 1 hit PS00136, SUBTILASE_ASP, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ORYZ_ASPFU | |
Accessioni | P28296Primary (citable) accession number: P28296 Secondary accession number(s): Q4WQ71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | December 6, 2005 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families