alp1

Functionⁱ

Secreted alkaline protease that allows assimilation of proteinaceous substrates. Acts as a significant virulence factor in invasive aspergillosis. Involved in immune evasion from the human and mice complement systems during infection. Efficiently cleaves important components of the complement cascade such as such as C3, C4, C5, and C1q, as well as IgG, which leads to down-regulation of complement activation at the hyphal surface.3 Publications

Catalytic activityⁱ

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.

pH dependenceⁱ

Optimum pH is 7-8.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	162	Charge relay systemBy similarity	1
Active siteⁱ	193	Charge relay systemBy similarity	1
Active siteⁱ	349	Charge relay systemBy similarity	1

GO - Molecular functionⁱ

fibrinogen binding
Source: AspGD
Inferred from direct assayⁱ
- 21870122
IgE binding
Source: UniProtKB
Inferred from direct assayⁱ
- 10677362
peptidase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 20498262
serine-type endopeptidase activity Source: InterPro
serine-type peptidase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 20303595

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

active evasion of host immune response via regulation of host complement system
Source: UniProtKB
Inferred from direct assayⁱ
- 20303595
complement activation
Source: AspGD
Inferred from mutant phenotypeⁱ
- 20498262
elastin catabolic process
Source: AspGD
Inferred from direct assayⁱ
- 8500876
pathogenesis
Source: UniProtKB
Inferred from direct assayⁱ
- 20303595
protein catabolic process
Source: AspGD
Inferred from mutant phenotypeⁱ
- 20498262

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Serine protease
Biological process	Virulence

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	S08.053

MEROPSⁱ

S08.053

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Alkaline protease 1 (EC:3.4.21.63) Short name: ALP Alternative name(s): Aspergillopeptidase B Aspergillus proteinase B Elastase Elastinolytic serine proteinase Oryzin Allergen: Asp f 13
Gene namesⁱ	Name:alp1 Synonyms:alk1, alp ORF Names:AFUA_4G11800
Organismⁱ	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifierⁱ	330879 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Neosartorya fumigata
Proteomesⁱ	UP000002530 Componentsⁱ: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

EuPathDBⁱ	FungiDB:Afu4g11800

EuPathDBⁱ

FungiDB:Afu4g11800

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Allergenic propertiesⁱ

Causes an allergic reaction in human. Binds to IgE via three immunodominant epitopes localized at the C-terminal part.

Keywords - Diseaseⁱ

Allergen

Protein family/group databases

Allergomeⁱ

3111 Asp f 13.0101
66 Asp f 13

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 21	By similarityAdd BLAST	21
PropeptideⁱPRO_0000027082	22 – 101	1 PublicationAdd BLAST	80
ChainⁱPRO_0000027083	102 – 403	Alkaline protease 1Add BLAST	302

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	253	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	307	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	367	N-linked (GlcNAc...) asparagineSequence analysis	1

Keywords - PTMⁱ

Glycoprotein, Zymogen

Expressionⁱ

Inductionⁱ

Expression is controlled by the prtT transcription factor.2 Publications

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	5085.CADAFUBP00006700

STRINGⁱ

5085.CADAFUBP00006700

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P28296
SMRⁱ	P28296
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	159 – 384	Peptidase S8Add BLAST		226

Sequence similaritiesⁱ

Belongs to the peptidase S8 family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

HOGENOMⁱ	HOG000199176
InParanoidⁱ	P28296
KEGG Orthology (KO) More...KOⁱ	K18549
OMAⁱ	LSAWYTS
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4A4B

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd04077 Peptidases_S8_PCSK9_Proteinase, 1 hit
Gene3Dⁱ	3.30.70.80, 1 hit 3.40.50.200, 1 hit
InterProⁱ	View protein in InterPro IPR034193 PCSK9_ProteinaseK-like IPR000209 Peptidase_S8/S53_dom IPR036852 Peptidase_S8/S53_dom_sf IPR023827 Peptidase_S8_Asp-AS IPR022398 Peptidase_S8_His-AS IPR023828 Peptidase_S8_Ser-AS IPR015500 Peptidase_S8_subtilisin-rel IPR010259 S8pro/Inhibitor_I9 IPR037045 S8pro/Inhibitor_I9_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF05922 Inhibitor_I9, 1 hit PF00082 Peptidase_S8, 1 hit
PRINTSⁱ	PR00723 SUBTILISIN
SUPFAMⁱ	SSF52743 SSF52743, 1 hit
PROSITEⁱ	View protein in PROSITE PS00136 SUBTILASE_ASP, 1 hit PS00137 SUBTILASE_HIS, 1 hit PS00138 SUBTILASE_SER, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P28296-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLSIKRTLLL LGAVLPAVFG APVQETRRAA QKIPGKYIVT FKPGTDTATI 
        60         70         80         90        100
ESHTLWATDL HKRNLERRDT TSGEPPVGIE KSYKIKDFAA YAGSFDDATI 
       110        120        130        140        150
EEIRKSADVA HVEEDQIWYL DALTTQKGAP WGLGSISHKG QASTDYIYDT 
       160        170        180        190        200
SAGAGTYAYV VDSGINVNHV EFESRASLAY NAAGGSHVDS IGHGTHVAGT 
       210        220        230        240        250
IGGKTYGVAK KTNLLSVKVF QGESSSTSII LDGFNWAVND IVSKGRTKKA 
       260        270        280        290        300
AINMSLGGGY SYAFNNAVEN AFDEGVLSVV AAGNENSDAS NTSPASAPNA 
       310        320        330        340        350
LTVAAINKSN ARASFSNYGS VVDIFAPGQD ILSAWIGSTT ATNTISGTSM 
       360        370        380        390        400
ATPHIVGLSV YLMGLENLSG PAAVTARIKE LATNGVVTNV KGSPNKLAYN 

GNA

Length:403

Mass (Da):42,190

Last modified:December 6, 2005 - v2

Checksum:ⁱ2D900D1AE22CDD4A

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	106 – 107	SA → RG in CAA77666 (PubMed:1601287).Curated		2
Sequence conflictⁱ	295	A → R in AAB07672 (PubMed:8500876).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z11580 Genomic DNA Translation: CAA77666.1 M99420 Genomic DNA Translation: AAB07672.1 AAHF01000005 Genomic DNA Translation: EAL89613.1
PIRⁱ	S22184
NCBI Reference Sequences More...RefSeqⁱ	XP_751651.1, XM_746558.1

Genome annotation databases

EnsemblFungiⁱ	EAL89613; EAL89613; AFUA_4G11800
GeneIDⁱ	3509271
KEGGⁱ	afm:AFUA_4G11800

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P28296	Alkaline protease 1		ASPFC		403	UniRef100_B0Y708
Uncharacterized protein		ASPFM		403
Alkaline protease (Fragment)		ASPFM		341

Protein	Similar proteins		Species	Score	Length	Source
P28296	Alkaline protease 1		NEOFI		403	UniRef90_A1CWF3
Alkaline protease 1		ASPFC		403
Uncharacterized protein		ASPFM		403
Alkaline protease 1		Aspergillus lentulus		403
Alkaline protease (Fragment)		Aspergillus sp. MK245		331
+10

Protein	Similar proteins		Species	Score	Length	Source
P28296	Alkaline protease 1		EMENI		403	UniRef50_Q00208
Subtilisin-like proteinase Mp1		MAGPO		404
Alkaline proteinase		HYPAT		409
Alkaline protease 1		NEOFI		403
Alkaline protease 1		ASPFC		403
+195

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z11580 Genomic DNA Translation: CAA77666.1 M99420 Genomic DNA Translation: AAB07672.1 AAHF01000005 Genomic DNA Translation: EAL89613.1
PIRⁱ	S22184
RefSeqⁱ	XP_751651.1, XM_746558.1

3D structure databases

ProteinModelPortalⁱ	P28296
SMRⁱ	P28296
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	5085.CADAFUBP00006700

STRINGⁱ

5085.CADAFUBP00006700

Protein family/group databases

Allergomeⁱ	3111 Asp f 13.0101 66 Asp f 13
MEROPSⁱ	S08.053

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	EAL89613; EAL89613; AFUA_4G11800
GeneIDⁱ	3509271
KEGGⁱ	afm:AFUA_4G11800

Organism-specific databases

EuPathDBⁱ	FungiDB:Afu4g11800

EuPathDBⁱ

FungiDB:Afu4g11800

Phylogenomic databases

HOGENOMⁱ	HOG000199176
InParanoidⁱ	P28296
KOⁱ	K18549
OMAⁱ	LSAWYTS
OrthoDBⁱ	EOG092C4A4B

Family and domain databases

CDDⁱ	cd04077 Peptidases_S8_PCSK9_Proteinase, 1 hit
Gene3Dⁱ	3.30.70.80, 1 hit 3.40.50.200, 1 hit
InterProⁱ	View protein in InterPro IPR034193 PCSK9_ProteinaseK-like IPR000209 Peptidase_S8/S53_dom IPR036852 Peptidase_S8/S53_dom_sf IPR023827 Peptidase_S8_Asp-AS IPR022398 Peptidase_S8_His-AS IPR023828 Peptidase_S8_Ser-AS IPR015500 Peptidase_S8_subtilisin-rel IPR010259 S8pro/Inhibitor_I9 IPR037045 S8pro/Inhibitor_I9_sf
Pfamⁱ	View protein in Pfam PF05922 Inhibitor_I9, 1 hit PF00082 Peptidase_S8, 1 hit
PRINTSⁱ	PR00723 SUBTILISIN
SUPFAMⁱ	SSF52743 SSF52743, 1 hit
PROSITEⁱ	View protein in PROSITE PS00136 SUBTILASE_ASP, 1 hit PS00137 SUBTILASE_HIS, 1 hit PS00138 SUBTILASE_SER, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ORYZ_ASPFU
Accessionⁱ	P28296Primary (citable) accession number: P28296 Secondary accession number(s): Q4WQ71
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
	Last sequence update:	December 6, 2005
	Last modified:	November 7, 2018
	This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

UniProtKB

UniParc

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Proteomes

Annotation systems

Supporting data

UniProtKB - P28296 (ORYZ_ASPFU)

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Alkaline protease 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Keywords - Diseasei

Protein family/group databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ