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Protein

Dihydroorotate dehydrogenase (fumarate)

Gene

URA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor. Molecular oxygen can replace fumarate in vitro. Does not use oxaloacetate or NAD or NADP as electron acceptors.6 Publications

Miscellaneous

Present with 264000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(S)-dihydroorotate + fumarate = orotate + succinate.1 Publication

Cofactori

FMN1 PublicationNote: Binds 1 FMN per subunit.1 Publication

Enzyme regulationi

The activity is independent of the presence of oxygen.

Kineticsi

  1. KM=8.4 µM for (S)-dihydroorotate (at pH 7.5)3 Publications
  2. KM=45 µM for fumarate (at pH 7.5)3 Publications
  3. KM=115 µM for 2,6-dichloroindophenol3 Publications
  1. Vmax=20.4 µmol/min/mg enzyme (with 2,6-dichloroindophenol as electron acceptor)3 Publications
  2. Vmax=5.1 µmol/min/mg enzyme (with fumarate as electron acceptor)3 Publications

pH dependencei

Optimum pH is 8.5. Active from pH 7 to pH 10.3 Publications

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in the pathway UMP biosynthesis via de novo pathway, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46SubstrateBy similarity1
Binding sitei130FMNBy similarity1
Binding sitei130SubstrateBy similarity1
Active sitei133NucleophileBy similarity1
Binding sitei167FMNBy similarity1
Binding sitei195FMN; via carbonyl oxygenBy similarity1
Binding sitei224FMN; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 47FMNBy similarity2
Nucleotide bindingi252 – 253FMNBy similarity2
Nucleotide bindingi274 – 275FMNBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyrimidine biosynthesis
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciMetaCyc:YKL216W-MONOMER
YEAST:YKL216W-MONOMER
ReactomeiR-SCE-500753 Pyrimidine biosynthesis
SABIO-RKiP28272
UniPathwayiUPA00070

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase (fumarate) (EC:1.3.98.1)
Short name:
DHOD
Short name:
DHODase
Short name:
DHOdehase
Alternative name(s):
Dihydroorotate oxidase
Gene namesi
Name:URA1
Ordered Locus Names:YKL216W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL216W
SGDiS000001699 URA1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5621

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485061 – 314Dihydroorotate dehydrogenase (fumarate)Add BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP28272
PaxDbiP28272
PRIDEiP28272

PTM databases

iPTMnetiP28272

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GFAPP141363EBI-14386,EBI-744302From Homo sapiens.

Protein-protein interaction databases

BioGridi33949, 104 interactors
DIPiDIP-6573N
IntActiP28272, 11 interactors
MINTiP28272
STRINGi4932.YKL216W

Chemistry databases

BindingDBiP28272

Structurei

3D structure databases

ProteinModelPortaliP28272
SMRiP28272
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 74Substrate bindingBy similarity5
Regioni196 – 197Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00500000044924
HOGENOMiHOG000225104
InParanoidiP28272
KOiK00226
OMAiSCPHAKG
OrthoDBiEOG092C37TJ

Family and domain databases

CDDicd04741 DHOD_1A_like, 1 hit
Gene3Di2.30.26.10, 2 hits
3.20.20.70, 3 hits
HAMAPiMF_00224 DHO_dh_type1, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033886 DHOD_1A
IPR023359 Dihydro_DH_chainA_dom2
IPR005720 Dihydroorotate_DH
IPR024920 Dihydroorotate_DH_1
IPR012135 Dihydroorotate_DH_1_2
IPR001295 Dihydroorotate_DH_CS
PfamiView protein in Pfam
PF01180 DHO_dh, 1 hit
PIRSFiPIRSF000164 DHO_oxidase, 1 hit
TIGRFAMsiTIGR01037 pyrD_sub1_fam, 1 hit
PROSITEiView protein in PROSITE
PS00911 DHODEHASE_1, 1 hit
PS00912 DHODEHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P28272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTASLTTKFL NNTYENPFMN ASGVHCMTTQ ELDELANSKA GAFITKSATT
60 70 80 90 100
LEREGNPEPR YISVPLGSIN SMGLPNEGID YYLSYVLNRQ KNYPDAPAIF
110 120 130 140 150
FSVAGMSIDE NLNLLRKIQD SEFNGITELN LSCPNVPGKP QVAYDFDLTK
160 170 180 190 200
ETLEKVFAFF KKPLGVKLPP YFDFAHFDIM AKILNEFPLA YVNSINSIGN
210 220 230 240 250
GLFIDVEKES VVVKPKNGFG GIGGEYVKPT ALANVRAFYT RLRPEIKVIG
260 270 280 290 300
TGGIKSGKDA FEHLLCGASM LQIGTELQKE GVKIFERIEK ELKDIMEAKG
310
YTSIDQFRGK LNSI
Length:314
Mass (Da):34,801
Last modified:December 1, 1992 - v1
Checksum:i0F1FF9BDA7F8D68E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti58E → K in strain: CLIB 95, CLIB 219, CLIB 382, CLIB 388, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13, YIIc12 and YIIc17. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83295 Genomic DNA Translation: AAA34566.1
X59371 Genomic DNA Translation: CAA42014.1
AJ585637 Genomic DNA Translation: CAE52157.1
AJ585638 Genomic DNA Translation: CAE52158.1
AJ585639 Genomic DNA Translation: CAE52159.1
AJ585640 Genomic DNA Translation: CAE52160.1
AJ585641 Genomic DNA Translation: CAE52161.1
AJ585642 Genomic DNA Translation: CAE52162.1
AJ585643 Genomic DNA Translation: CAE52163.1
AJ585644 Genomic DNA Translation: CAE52164.1
AJ585645 Genomic DNA Translation: CAE52165.1
AJ585646 Genomic DNA Translation: CAE52166.1
AJ585647 Genomic DNA Translation: CAE52167.1
AJ585648 Genomic DNA Translation: CAE52168.1
AJ585649 Genomic DNA Translation: CAE52169.1
AJ585650 Genomic DNA Translation: CAE52170.1
AJ585651 Genomic DNA Translation: CAE52171.1
X75951 Genomic DNA Translation: CAA53557.1
Z28216 Genomic DNA Translation: CAA82061.1
BK006944 Genomic DNA Translation: DAA08953.1
PIRiJC1276
RefSeqiNP_012706.1, NM_001179781.1

Genome annotation databases

EnsemblFungiiYKL216W; YKL216W; YKL216W
GeneIDi853664
KEGGisce:YKL216W

Similar proteinsi

Entry informationi

Entry nameiPYRD_YEAST
AccessioniPrimary (citable) accession number: P28272
Secondary accession number(s): D6VWY7, Q2XN75, Q70DC7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 18, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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