UniProtKB - P28246 (BCR_ECOLI)
Protein
Bicyclomycin resistance protein
Gene
bcr
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance (PubMed:2694948). Probable membrane translocase. A transporter able to export peptides. When overexpressed, allows cells deleted for multiple peptidases (pepA, pepB, pepD and pepN) to grow in the presence of dipeptides Ala-Gln or Gly-Tyr which otherwise inhibit growth (PubMed:20067529). Cells overexpressing this protein have decreased intracellular levels of Ala-Gln dipeptide, and in a system that produces the Ala-Gln dipeptide overproduction of this protein increases export of the dipeptide (PubMed:20067529).1 Publication
GO - Molecular functioni
- cysteine transmembrane transporter activity Source: EcoCyc
- dipeptide transmembrane transporter activity Source: EcoCyc
- xenobiotic transmembrane transporter activity Source: GO_Central
GO - Biological processi
- cysteine export across plasma membrane Source: EcoCyc
- dipeptide transmembrane transport Source: EcoCyc
- protein transport Source: UniProtKB-KW
- response to antibiotic Source: UniProtKB-KW
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: EcoCyc
Keywordsi
Biological process | Antibiotic resistance, Peptide transport, Protein transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:BCR-MONOMER MetaCyc:BCR-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.2.7, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Bicyclomycin resistance proteinAlternative name(s): Sulfonamide resistance protein |
Gene namesi | Name:bcr Synonyms:bicA, bicR, sur1 Publication, suxA Ordered Locus Names:b2182, JW5363 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 26 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 27 – 48 | PeriplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 49 – 65 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 66 – 76 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 77 – 98 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 99 – 105 | PeriplasmicSequence analysis | 7 | |
Transmembranei | 106 – 122 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 123 – 137 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 138 – 159 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 160 – 165 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 166 – 185 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 186 – 214 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 215 – 239 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 240 – 250 | PeriplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 251 – 268 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 269 – 285 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 286 – 302 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 303 – 308 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 309 – 332 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 333 – 344 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 345 – 366 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 367 – 372 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 373 – 390 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 391 – 396 | Cytoplasmic1 Publication | 6 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173315 | 1 – 396 | Bicyclomycin resistance proteinAdd BLAST | 396 |
Proteomic databases
PaxDbi | P28246 |
PRIDEi | P28246 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260471, 162 interactors |
STRINGi | 511145.b2182 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2814, Bacteria |
HOGENOMi | CLU_001265_47_0_6 |
InParanoidi | P28246 |
PhylomeDBi | P28246 |
Family and domain databases
InterProi | View protein in InterPro IPR004812, Efflux_drug-R_Bcr/CmlA IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR004734, Multidrug-R |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00880, 2_A_01_02, 1 hit TIGR00710, efflux_Bcr_CflA, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P28246-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTRQHSSFA IVFILGLLAM LMPLSIDMYL PALPVISAQF GVPAGSTQMT
60 70 80 90 100
LSTYILGFAL GQLIYGPMAD SFGRKPVVLG GTLVFAAAAV ACALANTIDQ
110 120 130 140 150
LIVMRFFHGL AAAAASVVIN ALMRDIYPKE EFSRMMSFVM LVTTIAPLMA
160 170 180 190 200
PIVGGWVLVW LSWHYIFWIL ALAAILASAM IFFLIKETLP PERRQPFHIR
210 220 230 240 250
TTIGNFAALF RHKRVLSYML ASGFSFAGMF SFLSAGPFVY IEINHVAPEN
260 270 280 290 300
FGYYFALNIV FLFVMTIFNS RFVRRIGALN MFRSGLWIQF IMAAWMVISA
310 320 330 340 350
LLGLGFWSLV VGVAAFVGCV SMVSSNAMAV ILDEFPHMAG TASSLAGTFR
360 370 380 390
FGIGAIVGAL LSLATFNSAW PMIWSIAFCA TSSILFCLYA SRPKKR
Sequence cautioni
The sequence AAA16406 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAA45230 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 99 | D → V in CAA45230 (PubMed:8486276).Curated | 1 | |
Sequence conflicti | 246 | V → I (Ref. 1) Curated | 1 | |
Sequence conflicti | 246 | V → I in CAA45230 (PubMed:8486276).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63703 Genomic DNA Translation: CAA45230.1 Different initiation. U00008 Genomic DNA Translation: AAA16406.1 Different initiation. U00096 Genomic DNA Translation: AAC75243.1 AP009048 Genomic DNA Translation: BAE76647.1 |
PIRi | E64987 |
RefSeqi | NP_416687.1, NC_000913.3 WP_000213361.1, NZ_SSZK01000027.1 |
Genome annotation databases
EnsemblBacteriai | AAC75243; AAC75243; b2182 BAE76647; BAE76647; BAE76647 |
GeneIDi | 57729695 944808 |
KEGGi | ecj:JW5363 eco:b2182 |
PATRICi | fig|1411691.4.peg.54 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63703 Genomic DNA Translation: CAA45230.1 Different initiation. U00008 Genomic DNA Translation: AAA16406.1 Different initiation. U00096 Genomic DNA Translation: AAC75243.1 AP009048 Genomic DNA Translation: BAE76647.1 |
PIRi | E64987 |
RefSeqi | NP_416687.1, NC_000913.3 WP_000213361.1, NZ_SSZK01000027.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260471, 162 interactors |
STRINGi | 511145.b2182 |
Protein family/group databases
TCDBi | 2.A.1.2.7, the major facilitator superfamily (mfs) |
Proteomic databases
PaxDbi | P28246 |
PRIDEi | P28246 |
Genome annotation databases
EnsemblBacteriai | AAC75243; AAC75243; b2182 BAE76647; BAE76647; BAE76647 |
GeneIDi | 57729695 944808 |
KEGGi | ecj:JW5363 eco:b2182 |
PATRICi | fig|1411691.4.peg.54 |
Organism-specific databases
EchoBASEi | EB1390 |
Phylogenomic databases
eggNOGi | COG2814, Bacteria |
HOGENOMi | CLU_001265_47_0_6 |
InParanoidi | P28246 |
PhylomeDBi | P28246 |
Enzyme and pathway databases
BioCyci | EcoCyc:BCR-MONOMER MetaCyc:BCR-MONOMER |
Miscellaneous databases
PROi | PR:P28246 |
Family and domain databases
InterProi | View protein in InterPro IPR004812, Efflux_drug-R_Bcr/CmlA IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR004734, Multidrug-R |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00880, 2_A_01_02, 1 hit TIGR00710, efflux_Bcr_CflA, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BCR_ECOLI | |
Accessioni | P28246Primary (citable) accession number: P28246 Secondary accession number(s): Q2MAQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | July 19, 2003 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families