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UniProtKB - P28230 (CXB1_MOUSE)
Protein
Gap junction beta-1 protein
Gene
Gjb1
Organism
Mus musculus (Mouse)
Status
Functioni
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
GO - Molecular functioni
- gap junction channel activity Source: MGI
- identical protein binding Source: MGI
GO - Biological processi
- cell-cell signaling Source: GO_Central
- epididymis development Source: Ensembl
- purine ribonucleotide transport Source: MGI
Enzyme and pathway databases
Reactomei | R-MMU-190704, Oligomerization of connexins into connexons |
Names & Taxonomyi
Protein namesi | Recommended name: Gap junction beta-1 proteinAlternative name(s): Connexin-32 Short name: Cx32 |
Gene namesi | Name:Gjb1 Synonyms:Cxn-32 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95719, Gjb1 |
VEuPathDBi | HostDB:ENSMUSG00000047797 |
Subcellular locationi
Plasma membrane
Other locations
Plasma Membrane
- connexin complex Source: GO_Central
- lateral plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- gap junction Source: MGI
- integral component of membrane Source: UniProtKB-KW
- protein-containing complex Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 23 – 45 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 46 – 75 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 76 – 95 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 96 – 130 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 131 – 153 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 154 – 191 | ExtracellularSequence analysisAdd BLAST | 38 | |
Transmembranei | 192 – 214 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 215 – 283 | CytoplasmicSequence analysisAdd BLAST | 69 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Gap junction, MembranePathology & Biotechi
Disruption phenotypei
Mice lacking both Gja12 and Gjb1 display a severe demyelination phenotype associated with oligodendrocyte death. These mice develop action tremors, tonic seizures, sporadic convulsions and loss of consciousness preceding death in the sixth week after birth.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057851 | 1 – 283 | Gap junction beta-1 proteinAdd BLAST | 283 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 233 | PhosphoserineBy similarity | 1 | |
Modified residuei | 258 | PhosphoserineCombined sources | 1 | |
Modified residuei | 266 | PhosphoserineCombined sources | 1 | |
Modified residuei | 277 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P28230 |
MaxQBi | P28230 |
PaxDbi | P28230 |
PRIDEi | P28230 |
ProteomicsDBi | 284073 |
PTM databases
iPTMneti | P28230 |
PhosphoSitePlusi | P28230 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000047797, Expressed in liver and 162 other tissues |
Genevisiblei | P28230, MM |
Interactioni
Subunit structurei
A connexon is composed of a hexamer of connexins.
Interacts with CNST.
1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
IntActi | P28230, 1 interactor |
STRINGi | 10090.ENSMUSP00000062723 |
Miscellaneous databases
RNActi | P28230, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502R1QN, Eukaryota |
GeneTreei | ENSGT01030000234513 |
HOGENOMi | CLU_037388_4_1_1 |
InParanoidi | P28230 |
OMAi | MGAWIQQ |
OrthoDBi | 1131301at2759 |
PhylomeDBi | P28230 |
TreeFami | TF329606 |
Family and domain databases
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002267, Connexin32 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01138, CONNEXINB1 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P28230-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNWTGLYTLL SGVNRHSTAI GRVWLSVIFI FRIMVLVVAA ESVWGDEKSS
60 70 80 90 100
FICNTLQPGC NSVCYDHFFP ISHVRLWSLQ LILVSTPALL VAMHVAHQQH
110 120 130 140 150
IEKKMLRLEG HGDPLHLEEV KRHKVHISGT LWWTYVISVV FRLLFEAVFM
160 170 180 190 200
YVFYLLYPGY AMVRLVKCEA FPCPNTVDCF VSRPTEKTVF TVFMLAASGI
210 220 230 240 250
CIILNVAEVV YLIIRACARR AQRRSNPPSR KGSGFGHRLS PEYKQNEINK
260 270 280
LLSEQDGSLK DILRRSPGTG AGLAEKSDRC SAC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 235 | F → S in AAA37296 (PubMed:2060697).Curated | 1 | |
Sequence conflicti | 263 – 266 | LRRS → PXPH in AAA37296 (PubMed:2060697).Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63802 mRNA Translation: AAA37296.2 M81447 Genomic DNA Translation: AAA37496.1 AJ271753 Genomic DNA Translation: CAB72446.1 BC026833 mRNA Translation: AAH26833.1 |
CCDSi | CCDS30314.1 |
PIRi | B49769 |
RefSeqi | NP_001289425.1, NM_001302496.1 NP_001289426.1, NM_001302497.1 NP_001289427.1, NM_001302498.1 NP_032150.2, NM_008124.3 |
Genome annotation databases
Ensembli | ENSMUST00000052130; ENSMUSP00000062723; ENSMUSG00000047797 ENSMUST00000119080; ENSMUSP00000113904; ENSMUSG00000047797 ENSMUST00000119190; ENSMUSP00000113516; ENSMUSG00000047797 |
GeneIDi | 14618 |
KEGGi | mmu:14618 |
UCSCi | uc009txk.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63802 mRNA Translation: AAA37296.2 M81447 Genomic DNA Translation: AAA37496.1 AJ271753 Genomic DNA Translation: CAB72446.1 BC026833 mRNA Translation: AAH26833.1 |
CCDSi | CCDS30314.1 |
PIRi | B49769 |
RefSeqi | NP_001289425.1, NM_001302496.1 NP_001289426.1, NM_001302497.1 NP_001289427.1, NM_001302498.1 NP_032150.2, NM_008124.3 |
3D structure databases
SMRi | P28230 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P28230, 1 interactor |
STRINGi | 10090.ENSMUSP00000062723 |
PTM databases
iPTMneti | P28230 |
PhosphoSitePlusi | P28230 |
Proteomic databases
jPOSTi | P28230 |
MaxQBi | P28230 |
PaxDbi | P28230 |
PRIDEi | P28230 |
ProteomicsDBi | 284073 |
Protocols and materials databases
Antibodypediai | 542, 568 antibodies from 31 providers |
DNASUi | 14618 |
Genome annotation databases
Ensembli | ENSMUST00000052130; ENSMUSP00000062723; ENSMUSG00000047797 ENSMUST00000119080; ENSMUSP00000113904; ENSMUSG00000047797 ENSMUST00000119190; ENSMUSP00000113516; ENSMUSG00000047797 |
GeneIDi | 14618 |
KEGGi | mmu:14618 |
UCSCi | uc009txk.2, mouse |
Organism-specific databases
CTDi | 2705 |
MGIi | MGI:95719, Gjb1 |
VEuPathDBi | HostDB:ENSMUSG00000047797 |
Phylogenomic databases
eggNOGi | ENOG502R1QN, Eukaryota |
GeneTreei | ENSGT01030000234513 |
HOGENOMi | CLU_037388_4_1_1 |
InParanoidi | P28230 |
OMAi | MGAWIQQ |
OrthoDBi | 1131301at2759 |
PhylomeDBi | P28230 |
TreeFami | TF329606 |
Enzyme and pathway databases
Reactomei | R-MMU-190704, Oligomerization of connexins into connexons |
Miscellaneous databases
BioGRID-ORCSi | 14618, 0 hits in 64 CRISPR screens |
ChiTaRSi | Gjb1, mouse |
PROi | PR:P28230 |
RNActi | P28230, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000047797, Expressed in liver and 162 other tissues |
Genevisiblei | P28230, MM |
Family and domain databases
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002267, Connexin32 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01138, CONNEXINB1 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CXB1_MOUSE | |
Accessioni | P28230Primary (citable) accession number: P28230 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | May 10, 2004 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families