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Protein

Gap junction beta-1 protein

Gene

Gjb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

GO - Molecular functioni

  • gap junction channel activity Source: MGI
  • protein homodimerization activity Source: MGI

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-190704 Oligomerization of connexins into connexons
R-MMU-190861 Gap junction assembly

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction beta-1 protein
Alternative name(s):
Connexin-32
Short name:
Cx32
Gene namesi
Name:Gjb1
Synonyms:Cxn-32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95719 Gjb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 45HelicalSequence analysisAdd BLAST23
Topological domaini46 – 75ExtracellularSequence analysisAdd BLAST30
Transmembranei76 – 95HelicalSequence analysisAdd BLAST20
Topological domaini96 – 130CytoplasmicSequence analysisAdd BLAST35
Transmembranei131 – 153HelicalSequence analysisAdd BLAST23
Topological domaini154 – 191ExtracellularSequence analysisAdd BLAST38
Transmembranei192 – 214HelicalSequence analysisAdd BLAST23
Topological domaini215 – 283CytoplasmicSequence analysisAdd BLAST69

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice lacking both Gja12 and Gjb1 display a severe demyelination phenotype associated with oligodendrocyte death. These mice develop action tremors, tonic seizures, sporadic convulsions and loss of consciousness preceding death in the sixth week after birth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000578511 – 283Gap junction beta-1 proteinAdd BLAST283

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233PhosphoserineBy similarity1
Modified residuei258PhosphoserineCombined sources1
Modified residuei266PhosphoserineCombined sources1
Modified residuei277PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28230
PaxDbiP28230
PRIDEiP28230

PTM databases

iPTMnetiP28230
PhosphoSitePlusiP28230

Miscellaneous databases

PMAP-CutDBiP28230

Expressioni

Gene expression databases

BgeeiENSMUSG00000047797
CleanExiMM_GJB1
GenevisibleiP28230 MM

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins. Interacts with CNST.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

IntActiP28230, 1 interactor
STRINGi10090.ENSMUSP00000062723

Structurei

3D structure databases

ProteinModelPortaliP28230
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFM8 Eukaryota
ENOG410Y7VN LUCA
GeneTreeiENSGT00910000144026
HOGENOMiHOG000231127
HOVERGENiHBG009576
InParanoidiP28230
KOiK07620
OMAiYLVVRAC
OrthoDBiEOG091G0FKH
PhylomeDBiP28230
TreeFamiTF329606

Family and domain databases

Gene3Di1.20.1440.80, 1 hit
InterProiView protein in InterPro
IPR000500 Connexin
IPR002267 Connexin32
IPR019570 Connexin_CCC
IPR017990 Connexin_CS
IPR013092 Connexin_N
IPR038359 Connexin_N_sf
PANTHERiPTHR11984 PTHR11984, 1 hit
PfamiView protein in Pfam
PF00029 Connexin, 1 hit
PRINTSiPR00206 CONNEXIN
PR01138 CONNEXINB1
SMARTiView protein in SMART
SM00037 CNX, 1 hit
SM01089 Connexin_CCC, 1 hit
PROSITEiView protein in PROSITE
PS00407 CONNEXINS_1, 1 hit
PS00408 CONNEXINS_2, 1 hit

Sequencei

Sequence statusi: Complete.

P28230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNWTGLYTLL SGVNRHSTAI GRVWLSVIFI FRIMVLVVAA ESVWGDEKSS
60 70 80 90 100
FICNTLQPGC NSVCYDHFFP ISHVRLWSLQ LILVSTPALL VAMHVAHQQH
110 120 130 140 150
IEKKMLRLEG HGDPLHLEEV KRHKVHISGT LWWTYVISVV FRLLFEAVFM
160 170 180 190 200
YVFYLLYPGY AMVRLVKCEA FPCPNTVDCF VSRPTEKTVF TVFMLAASGI
210 220 230 240 250
CIILNVAEVV YLIIRACARR AQRRSNPPSR KGSGFGHRLS PEYKQNEINK
260 270 280
LLSEQDGSLK DILRRSPGTG AGLAEKSDRC SAC
Length:283
Mass (Da):32,004
Last modified:May 10, 2004 - v3
Checksum:iC79FC46AA13BC5D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235F → S in AAA37296 (PubMed:2060697).Curated1
Sequence conflicti263 – 266LRRS → PXPH in AAA37296 (PubMed:2060697).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63802 mRNA Translation: AAA37296.2
M81447 Genomic DNA Translation: AAA37496.1
AJ271753 Genomic DNA Translation: CAB72446.1
BC026833 mRNA Translation: AAH26833.1
CCDSiCCDS30314.1
PIRiB49769
RefSeqiNP_001289425.1, NM_001302496.1
NP_001289426.1, NM_001302497.1
NP_001289427.1, NM_001302498.1
NP_032150.2, NM_008124.3
UniGeneiMm.21198

Genome annotation databases

EnsembliENSMUST00000052130; ENSMUSP00000062723; ENSMUSG00000047797
ENSMUST00000119080; ENSMUSP00000113904; ENSMUSG00000047797
ENSMUST00000119190; ENSMUSP00000113516; ENSMUSG00000047797
GeneIDi14618
KEGGimmu:14618
UCSCiuc009txk.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCXB1_MOUSE
AccessioniPrimary (citable) accession number: P28230
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: May 10, 2004
Last modified: June 20, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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