Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5-hydroxytryptamine receptor 1B

Gene

HTR1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.12 Publications

Miscellaneous

A residue in the 7th transmembrane region (Thr-355 in human, 'Asn-351' in mouse and rat) is important for species-specific sensitivity to various agonists.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei355Important for species-specific agonist sensitivity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Enzyme and pathway databases

ReactomeiR-HSA-390666 Serotonin receptors
R-HSA-418594 G alpha (i) signalling events

Protein family/group databases

TCDBi9.A.14.3.6 the g-protein-coupled receptor (gpcr) family

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1B
Short name:
5-HT-1B
Short name:
5-HT1B
Alternative name(s):
S12
Serotonin 1D beta receptor
Short name:
5-HT-1D-beta
Serotonin receptor 1B
Gene namesi
Name:HTR1B
Synonyms:HTR1DB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000135312.5
HGNCiHGNC:5287 HTR1B
MIMi182131 gene
neXtProtiNX_P28222

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 49ExtracellularAdd BLAST49
Transmembranei50 – 75Helical; Name=1Add BLAST26
Topological domaini76 – 84Cytoplasmic9
Transmembranei85 – 110Helical; Name=2Add BLAST26
Topological domaini111 – 123ExtracellularAdd BLAST13
Transmembranei124 – 145Helical; Name=3Add BLAST22
Topological domaini146 – 165CytoplasmicAdd BLAST20
Transmembranei166 – 187Helical; Name=4Add BLAST22
Topological domaini188 – 205ExtracellularAdd BLAST18
Transmembranei206 – 228Helical; Name=5Add BLAST23
Topological domaini229 – 315CytoplasmicAdd BLAST87
Transmembranei316 – 336Helical; Name=6Add BLAST21
Topological domaini337 – 349ExtracellularAdd BLAST13
Transmembranei350 – 371Helical; Name=7Add BLAST22
Topological domaini372 – 390CytoplasmicAdd BLAST19

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi126L → A: No effect on agonist binding. 1 Publication1
Mutagenesisi129D → A: Abolishes agonist binding. 1 Publication1
Mutagenesisi130I → A: Abolishes agonist binding. 1 Publication1
Mutagenesisi133C → A: Abolishes agonist binding. 1 Publication1
Mutagenesisi134T → A: Slightly decreases agonist binding. 1 Publication1
Mutagenesisi200V → A: No effect on agonist binding. 1 Publication1
Mutagenesisi201V → A: No effect on agonist binding. 1 Publication1
Mutagenesisi203T → A: No effect on agonist binding. 1 Publication1
Mutagenesisi209T → A: No effect on agonist binding. 1 Publication1
Mutagenesisi212S → A: No effect on agonist binding. 1 Publication1
Mutagenesisi216A → S: No effect on agonist binding. 1 Publication1
Mutagenesisi327W → A: Abolishes agonist binding. 1 Publication1
Mutagenesisi330F → A: Abolishes agonist binding. 1 Publication1
Mutagenesisi331F → A: No effect on agonist binding. 1 Publication1
Mutagenesisi334S → A: No effect on agonist binding. 1 Publication1
Mutagenesisi337M → A: No effect on agonist binding. 1 Publication1
Mutagenesisi351F → A: No effect on agonist binding. 1 Publication1
Mutagenesisi352D → A: No effect on agonist binding. 1 Publication1
Mutagenesisi355T → A: No effect on agonist binding. 1 Publication1
Mutagenesisi359Y → A: No effect on agonist binding. 1 Publication1

Organism-specific databases

DisGeNETi3351
OpenTargetsiENSG00000135312
PharmGKBiPA29549

Chemistry databases

ChEMBLiCHEMBL1898
DrugBankiDB00918 Almotriptan
DB00321 Amitriptyline
DB00543 Amoxapine
DB00714 Apomorphine
DB01238 Aripiprazole
DB06216 Asenapine
DB08807 Bopindolol
DB01200 Bromocriptine
DB00248 Cabergoline
DB00363 Clozapine
DB04884 Dapoxetine
DB00320 Dihydroergotamine
DB00216 Eletriptan
DB01049 Ergoloid mesylate
DB00696 Ergotamine
DB00998 Frovatriptan
DB01221 Ketamine
DB00589 Lisuride
DB00408 Loxapine
DB05743 MAP-0004
DB00247 Methysergide
DB00952 Naratriptan
DB06096 NXN-188
DB00334 Olanzapine
DB00904 Ondansetron
DB01359 Penbutolol
DB01186 Pergolide
DB00960 Pindolol
DB00413 Pramipexole
DB00571 Propranolol
DB01224 Quetiapine
DB00953 Rizatriptan
DB00268 Ropinirole
DB00669 Sumatriptan
DB13025 Tiapride
DB09068 Vortioxetine
DB01392 Yohimbine
DB00246 Ziprasidone
DB00315 Zolmitriptan
GuidetoPHARMACOLOGYi2

Polymorphism and mutation databases

BioMutaiHTR1B
DMDMi112821

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689161 – 3905-hydroxytryptamine receptor 1BAdd BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi32N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi122 ↔ 199PROSITE-ProRule annotation1 Publication
Lipidationi388S-palmitoyl cysteineSequence analysis1

Post-translational modificationi

Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation.1 Publication
Palmitoylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP28222
PRIDEiP28222
ProteomicsDBi54452

PTM databases

iPTMnetiP28222
PhosphoSitePlusiP28222

Expressioni

Tissue specificityi

Detected in cerebral artery smooth muscle cells (at protein level). Detected in brain cortex, striatum, amygdala, medulla, hippocampus, caudate nucleus and putamen.3 Publications

Gene expression databases

BgeeiENSG00000135312 Expressed in 72 organ(s), highest expression level in popliteal artery
CleanExiHS_HTR1B
ExpressionAtlasiP28222 baseline and differential
GenevisibleiP28222 HS

Organism-specific databases

HPAiHPA028808

Interactioni

Subunit structurei

Homodimer. Heterodimer with HTR1D.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MDFIQ997506EBI-1056863,EBI-724076

Protein-protein interaction databases

BioGridi109583, 5 interactors
CORUMiP28222
IntActiP28222, 6 interactors
STRINGi9606.ENSP00000358963

Chemistry databases

BindingDBiP28222

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP28222
SMRiP28222
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 134Agonist binding10
Regioni327 – 331Agonist binding5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 148DRY motif; important for ligand-induced conformation changes and signaling3
Motifi365 – 369NPxxY motif; important for ligand-induced conformation changes and signaling5

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.1 Publication

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118795
HOGENOMiHOG000239242
HOVERGENiHBG106962
InParanoidiP28222
KOiK04153
OMAiACWFHMA
OrthoDBiEOG091G06VI
PhylomeDBiP28222
TreeFamiTF316350

Family and domain databases

InterProiView protein in InterPro
IPR002147 5HT1B_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF16 PTHR24247:SF16, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00513 5HT1BRECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P28222-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEPGAQCAP PPPAGSETWV PQANLSSAPS QNCSAKDYIY QDSISLPWKV
60 70 80 90 100
LLVMLLALIT LATTLSNAFV IATVYRTRKL HTPANYLIAS LAVTDLLVSI
110 120 130 140 150
LVMPISTMYT VTGRWTLGQV VCDFWLSSDI TCCTASILHL CVIALDRYWA
160 170 180 190 200
ITDAVEYSAK RTPKRAAVMI ALVWVFSISI SLPPFFWRQA KAEEEVSECV
210 220 230 240 250
VNTDHILYTV YSTVGAFYFP TLLLIALYGR IYVEARSRIL KQTPNRTGKR
260 270 280 290 300
LTRAQLITDS PGSTSSVTSI NSRVPDVPSE SGSPVYVNQV KVRVSDALLE
310 320 330 340 350
KKKLMAARER KATKTLGIIL GAFIVCWLPF FIISLVMPIC KDACWFHLAI
360 370 380 390
FDFFTWLGYL NSLINPIIYT MSNEDFKQAF HKLIRFKCTS
Length:390
Mass (Da):43,568
Last modified:December 1, 1992 - v1
Checksum:iCD874DC7EB44CF12
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_011715124F → C1 PublicationCorresponds to variant dbSNP:rs130060Ensembl.1
Natural variantiVAR_011831219F → L. Corresponds to variant dbSNP:rs130061Ensembl.1
Natural variantiVAR_011832367I → V. Corresponds to variant dbSNP:rs130063Ensembl.1
Natural variantiVAR_011833374E → K. Corresponds to variant dbSNP:rs130064Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89478 Genomic DNA No translation available.
D10995 Genomic DNA Translation: BAA01763.1
M83180 Genomic DNA Translation: AAA36029.1
L09732 Genomic DNA Translation: AAA36030.1
M81590 mRNA Translation: AAA60316.1
M75128 Genomic DNA Translation: AAA58675.1
AB041370 Genomic DNA Translation: BAA94455.1
AY225227 Genomic DNA Translation: AAO67712.1
AL049595 Genomic DNA No translation available.
BC069065 mRNA Translation: AAH69065.1
BC096206 mRNA Translation: AAH96206.1
BC096207 mRNA Translation: AAH96207.1
BC096208 mRNA Translation: AAH96208.1
CCDSiCCDS4986.1
PIRiJN0268
RefSeqiNP_000854.1, NM_000863.2
UniGeneiHs.123016

Genome annotation databases

EnsembliENST00000369947; ENSP00000358963; ENSG00000135312
GeneIDi3351
KEGGihsa:3351

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89478 Genomic DNA No translation available.
D10995 Genomic DNA Translation: BAA01763.1
M83180 Genomic DNA Translation: AAA36029.1
L09732 Genomic DNA Translation: AAA36030.1
M81590 mRNA Translation: AAA60316.1
M75128 Genomic DNA Translation: AAA58675.1
AB041370 Genomic DNA Translation: BAA94455.1
AY225227 Genomic DNA Translation: AAO67712.1
AL049595 Genomic DNA No translation available.
BC069065 mRNA Translation: AAH69065.1
BC096206 mRNA Translation: AAH96206.1
BC096207 mRNA Translation: AAH96207.1
BC096208 mRNA Translation: AAH96208.1
CCDSiCCDS4986.1
PIRiJN0268
RefSeqiNP_000854.1, NM_000863.2
UniGeneiHs.123016

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G1Xmodel-A1-390[»]
4IAQX-ray2.80A33-239[»]
A304-390[»]
4IARX-ray2.70A33-239[»]
A306-390[»]
5V54X-ray3.90A/B37-390[»]
6G79electron microscopy3.78S34-390[»]
ProteinModelPortaliP28222
SMRiP28222
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109583, 5 interactors
CORUMiP28222
IntActiP28222, 6 interactors
STRINGi9606.ENSP00000358963

Chemistry databases

BindingDBiP28222
ChEMBLiCHEMBL1898
DrugBankiDB00918 Almotriptan
DB00321 Amitriptyline
DB00543 Amoxapine
DB00714 Apomorphine
DB01238 Aripiprazole
DB06216 Asenapine
DB08807 Bopindolol
DB01200 Bromocriptine
DB00248 Cabergoline
DB00363 Clozapine
DB04884 Dapoxetine
DB00320 Dihydroergotamine
DB00216 Eletriptan
DB01049 Ergoloid mesylate
DB00696 Ergotamine
DB00998 Frovatriptan
DB01221 Ketamine
DB00589 Lisuride
DB00408 Loxapine
DB05743 MAP-0004
DB00247 Methysergide
DB00952 Naratriptan
DB06096 NXN-188
DB00334 Olanzapine
DB00904 Ondansetron
DB01359 Penbutolol
DB01186 Pergolide
DB00960 Pindolol
DB00413 Pramipexole
DB00571 Propranolol
DB01224 Quetiapine
DB00953 Rizatriptan
DB00268 Ropinirole
DB00669 Sumatriptan
DB13025 Tiapride
DB09068 Vortioxetine
DB01392 Yohimbine
DB00246 Ziprasidone
DB00315 Zolmitriptan
GuidetoPHARMACOLOGYi2

Protein family/group databases

TCDBi9.A.14.3.6 the g-protein-coupled receptor (gpcr) family
GPCRDBiSearch...

PTM databases

iPTMnetiP28222
PhosphoSitePlusiP28222

Polymorphism and mutation databases

BioMutaiHTR1B
DMDMi112821

Proteomic databases

PaxDbiP28222
PRIDEiP28222
ProteomicsDBi54452

Protocols and materials databases

DNASUi3351
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369947; ENSP00000358963; ENSG00000135312
GeneIDi3351
KEGGihsa:3351

Organism-specific databases

CTDi3351
DisGeNETi3351
EuPathDBiHostDB:ENSG00000135312.5
GeneCardsiHTR1B
HGNCiHGNC:5287 HTR1B
HPAiHPA028808
MIMi182131 gene
neXtProtiNX_P28222
OpenTargetsiENSG00000135312
PharmGKBiPA29549
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118795
HOGENOMiHOG000239242
HOVERGENiHBG106962
InParanoidiP28222
KOiK04153
OMAiACWFHMA
OrthoDBiEOG091G06VI
PhylomeDBiP28222
TreeFamiTF316350

Enzyme and pathway databases

ReactomeiR-HSA-390666 Serotonin receptors
R-HSA-418594 G alpha (i) signalling events

Miscellaneous databases

GeneWikii5-HT1B_receptor
GenomeRNAii3351
PROiPR:P28222
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135312 Expressed in 72 organ(s), highest expression level in popliteal artery
CleanExiHS_HTR1B
ExpressionAtlasiP28222 baseline and differential
GenevisibleiP28222 HS

Family and domain databases

InterProiView protein in InterPro
IPR002147 5HT1B_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF16 PTHR24247:SF16, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00513 5HT1BRECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5HT1B_HUMAN
AccessioniPrimary (citable) accession number: P28222
Secondary accession number(s): Q4VAY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 7, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  7. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again