UniProtKB - P28159 (SUH_DROME)
Protein
Suppressor of hairless protein
Gene
Su(H)
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations (PubMed:21262215, PubMed:1617729, PubMed:8674407, PubMed:10673509). Binds directly the 5'-GTGRGAR-3' DNA consensus sequence, which is present in the regulatory region of several genes (PubMed:10673509). Acts as a transcriptional repressor when it is not associated with Notch proteins (PubMed:10673509). When associated with some Notch protein, it acts as a transcriptional activator that activates transcription of Notch target genes (PubMed:10673509). Required for transcription of Sim (PubMed:10673509). Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence (PubMed:1744127, PubMed:1617730, PubMed:1617729). Required for neurogenesis in imaginal disks (PubMed:1617730, PubMed:7813798). In the larval brain, might play a role as a transducer of Notch signaling during type II neuroblast development (PubMed:21262215). Also functions independently of the Notch pathway, in the development of the bristle sensory organ precursor cell (PubMed:12642500).8 Publications
Caution
Despite some similarity with the 'phage' integrase family, PubMed:7958912 showed that it has no recombinase activity.Curated
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: FlyBase
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: FlyBase
- histone acetyltransferase binding Source: FlyBase
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: FlyBase
- RNA polymerase II-specific DNA-binding transcription factor binding Source: FlyBase
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: FlyBase
GO - Biological processi
- asymmetric cell division Source: FlyBase
- cell fate determination Source: FlyBase
- crystal cell differentiation Source: FlyBase
- hemopoiesis Source: FlyBase
- imaginal disc-derived leg morphogenesis Source: FlyBase
- imaginal disc-derived wing vein morphogenesis Source: FlyBase
- long-term memory Source: FlyBase
- negative regulation of compound eye cone cell fate specification Source: FlyBase
- negative regulation of Notch signaling pathway Source: FlyBase
- negative regulation of transcription, DNA-templated Source: FlyBase
- negative regulation of transcription by RNA polymerase II Source: FlyBase
- Notch signaling pathway Source: FlyBase
- positive regulation of G1/S transition of mitotic cell cycle Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- sensory organ precursor cell fate determination Source: UniProtKB
- wing disc dorsal/ventral pattern formation Source: FlyBase
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Repressor |
Biological process | Notch signaling pathway, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DME-350054, Notch-HLH transcription pathway |
SignaLinki | P28159 |
Names & Taxonomyi
Protein namesi | Recommended name: Suppressor of hairless proteinAlternative name(s): J kappa-recombination signal-binding protein RBP-J kappa |
Gene namesi | Name:Su(H) Synonyms:dRBP-JK ORF Names:CG3497 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0004837, Su(H) |
Subcellular locationi
Nucleus
- CSL-Notch-Mastermind transcription factor complex Source: FlyBase
- nucleus Source: FlyBase
- RNA polymerase II transcription repressor complex Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- polytene chromosome Source: FlyBase
- protein-containing complex Source: FlyBase
- transcription repressor complex Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Lethal during the first day of pupal development (PubMed:1617730). At the larval stage, results in a 'neurogenic' phenotype in imaginal disks, in which too many cells adopt the sensory organ precursor cell fate (PubMed:1617730). RNAi-mediated knockdown in type II neuroblasts, results in smaller cells (PubMed:1617730).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 315 | Y → F: No effect. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208572 | 1 – 594 | Suppressor of hairless proteinAdd BLAST | 594 |
Proteomic databases
PaxDbi | P28159 |
PTM databases
iPTMneti | P28159 |
Expressioni
Developmental stagei
Expressed in early syncytial embryo (PubMed:1617730, PubMed:1617729). During cellularization, transient accumulation in a striped pattern (PubMed:1617730, PubMed:1617729). From late stage 11 onward, expressed gradually in a small number of segmentally reiterated cells of the peripheral nervous system (PNS) (PubMed:1617730, PubMed:1617729). This expression is specific to the external sensory organs (PubMed:1617730). In the thoracic and abdominal segments, expressed in the trichogen and tormogen cells, two outer accessory cells present in all larval external sensory organs (PubMed:1617730). During the late third larval instar, expressed in many larval and imaginal tissues (PubMed:1617730). Broadly distributed in the disks, but most abundant in the posterior region of the wing pouch (PubMed:1617730). In the leg disk, predominant in a zone largely overlapping the posterior region (PubMed:1617730). In the eye-antenna disk, low level in both the retinal field posterior to the morphogenetic furrow and the central antenna region, while higher levels appears on the margins of the disk and in the vicinity of the morphogenetic furrow (PubMed:1617730). In pupae, expressed in many tissues at this stage, including developing muscle and epidermis (PubMed:1617730). In both microchaetes and macrochaetes of 24 hr pupae, expressed on the notum and the head (PubMed:1617730). Expressed in the tormogen cell and in specific bristle cells (PubMed:1617730).2 Publications
Gene expression databases
Bgeei | FBgn0004837, Expressed in second segment of antenna (Drosophila) and 68 other tissues |
ExpressionAtlasi | P28159, baseline and differential |
Genevisiblei | P28159, DM |
Interactioni
Subunit structurei
Interacts with activated cleaved Notch.
Interacts with Hairless, this interaction preventing its DNA-binding activity.
Interacts with insv (via BEN domain).
2 PublicationsBinary interactionsi
Hide detailsP28159
With | #Exp. | IntAct |
---|---|---|
insv [Q8SYK5] | 4 | EBI-92180,EBI-192407 |
N [P07207] | 7 | EBI-92180,EBI-103438 |
Notch1 [Q01705] from Mus musculus. | 3 | EBI-92180,EBI-1392707 |
GO - Molecular functioni
- histone acetyltransferase binding Source: FlyBase
- RNA polymerase II-specific DNA-binding transcription factor binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 60899, 85 interactors |
DIPi | DIP-177N |
IntActi | P28159, 22 interactors |
MINTi | P28159 |
STRINGi | 7227.FBpp0080261 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28159 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 429 – 519 | IPT/TIGAdd BLAST | 91 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 131 – 141 | DNA bindingBy similarityAdd BLAST | 11 | |
Regioni | 239 – 244 | DNA bindingBy similarity | 6 | |
Regioni | 266 – 271 | DNA bindingBy similarity | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 35 – 42 | Gln-rich | 8 | |
Compositional biasi | 58 – 88 | Gln-richAdd BLAST | 31 | |
Compositional biasi | 541 – 558 | Asn-richAdd BLAST | 18 | |
Compositional biasi | 569 – 576 | Gln-rich | 8 |
Sequence similaritiesi
Belongs to the Su(H) family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3743, Eukaryota |
HOGENOMi | CLU_022207_2_1_1 |
InParanoidi | P28159 |
OMAi | DMPHFGL |
OrthoDBi | 444988at2759 |
PhylomeDBi | P28159 |
Family and domain databases
CDDi | cd01176, IPT_RBP-Jkappa, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 2.60.40.1450, 1 hit |
InterProi | View protein in InterPro IPR015350, Beta-trefoil_DNA-bd_dom IPR036358, BTD_sf IPR040159, CLS_fam IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR008967, p53-like_TF_DNA-bd IPR015351, RBP-J/Cbf11/Cbf12_DNA-bd IPR037095, RBP-J/Cbf11/Cbf12_DNA-bd_sf IPR038007, RBP-Jkappa_IPT |
PANTHERi | PTHR10665, PTHR10665, 1 hit |
Pfami | View protein in Pfam PF09270, BTD, 1 hit PF09271, LAG1-DNAbind, 1 hit |
SMARTi | View protein in SMART SM01268, BTD, 1 hit SM01267, LAG1_DNAbind, 1 hit |
SUPFAMi | SSF110217, SSF110217, 1 hit SSF49417, SSF49417, 1 hit SSF81296, SSF81296, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P28159-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSYSQFNLN AAAPPAIAYE TTVVNPNGSP LDPHQQQQQQ SQDMPHFGLP
60 70 80 90 100
GPQPPSSQQQ QQQLQVHHQQ QQQQQQQQQQ QQHQQQMQMS LLPGPYRPHI
110 120 130 140 150
EEKKLTRDAM EKYMRERNDM VIVILHAKVA QKSYGNEKRF FCPPPCIYLF
160 170 180 190 200
GSGWRRRYEE MLQQGEGEQG AQLCAFIGIG SSDQDMQQLD LNGKQYCAAK
210 220 230 240 250
TLFISDSDKR KHFMLSVKMF YGNGHDIGVF NSKRIKVISK PSKKKQSLKN
260 270 280 290 300
ADLCIASGTN VALFNRLRSQ TVSTRYLHVE NGHFHASSTQ WGAFTIHLLD
310 320 330 340 350
DNESESEEFQ VRDGYIHYGA TVKLVCSVTG MALPRLIIRK VDKQMALLEA
360 370 380 390 400
DDPVSQLHKC AFYMKDTDRM YLCLSQEKII QFQATPCPKE PNKEMINDGA
410 420 430 440 450
CWTIISTDKA EYQFYEGMGP VASPVTPVPI VNSLNLNGGG DVAMLELSGD
460 470 480 490 500
NFTPHLQVWF GDVEAETMYR CTETLLCVVP EISQFRGEWL WVRQPTQVPI
510 520 530 540 550
SLVRNDGIIY ATGLTFTYTP EPGPRPHCNT QAEDVMRARQ NNNNNNITSI
560 570 580 590
SNNNNSNNAG SPAAGGGLQQ QQQQHQALPS ISEVQWNSHG SGLS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX2JAI1 | X2JAI1_DROME | Suppressor of hairless, isoform C | Su(H) anon-WO0257455.3, BG:DS00929.10, br7, C, CBF1 | 594 | Annotation score: |
Sequence cautioni
The sequence AAA06211 differs from that shown. Reason: Frameshift.Curated
The sequence CAA41282 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 116 – 117 | ER → DG in AAA06211 (PubMed:1744127).Curated | 2 | |
Sequence conflicti | 116 – 117 | ER → DG in CAA41282 (PubMed:1744127).Curated | 2 | |
Sequence conflicti | 492 | V → A in AAA06211 (PubMed:1744127).Curated | 1 | |
Sequence conflicti | 492 | V → A in CAA41282 (PubMed:1744127).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 80 – 82 | Missing in strain: LAMTO 3, LAMTO 4, LAMTO 12, LAMTO 18, LAMTO 19, LAMTO 21, LAMTO 31, LAMTO 35, LAMTO 101 and LAMTO 111. | 3 | |
Natural varianti | 81 – 82 | Missing in strain: LAMTO 13, LAMTO 37 and LAMTO 134. | 2 | |
Natural varianti | 82 | Missing in strain: LAMTO 124. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S69213 Genomic DNA Translation: AAA06211.1 Frameshift. X58393 mRNA Translation: CAA41282.1 Different initiation. M94383 mRNA Translation: AAA28919.1 AE014134 Genomic DNA Translation: AAF53434.1 AY069091 mRNA Translation: AAL39236.1 AF088255 Genomic DNA Translation: AAD39698.1 AF088256 Genomic DNA Translation: AAD39699.1 AF088257 Genomic DNA Translation: AAD39700.1 AF088258 Genomic DNA Translation: AAD39701.1 AF088259 Genomic DNA Translation: AAD39702.1 AF088260 Genomic DNA Translation: AAD39703.1 AF088261 Genomic DNA Translation: AAD39704.1 AF088262 Genomic DNA Translation: AAD39705.1 AF088263 Genomic DNA Translation: AAD39706.1 AF088264 Genomic DNA Translation: AAD39707.1 AF088265 Genomic DNA Translation: AAD39708.1 AF088266 Genomic DNA Translation: AAD39709.1 AF088267 Genomic DNA Translation: AAD39710.1 AF088268 Genomic DNA Translation: AAD39711.1 AF088269 Genomic DNA Translation: AAD39712.1 AF088270 Genomic DNA Translation: AAD39713.1 AF088271 Genomic DNA Translation: AAD39714.1 AF088272 Genomic DNA Translation: AAD39715.1 AF088273 Genomic DNA Translation: AAD39716.1 AF088274 Genomic DNA Translation: AAD39717.1 |
PIRi | A41585 A42770 |
RefSeqi | NP_001285948.1, NM_001299019.1 NP_476868.1, NM_057520.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0080700; FBpp0080261; FBgn0004837 FBtr0346621; FBpp0312201; FBgn0004837 |
GeneIDi | 34881 |
KEGGi | dme:Dmel_CG3497 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S69213 Genomic DNA Translation: AAA06211.1 Frameshift. X58393 mRNA Translation: CAA41282.1 Different initiation. M94383 mRNA Translation: AAA28919.1 AE014134 Genomic DNA Translation: AAF53434.1 AY069091 mRNA Translation: AAL39236.1 AF088255 Genomic DNA Translation: AAD39698.1 AF088256 Genomic DNA Translation: AAD39699.1 AF088257 Genomic DNA Translation: AAD39700.1 AF088258 Genomic DNA Translation: AAD39701.1 AF088259 Genomic DNA Translation: AAD39702.1 AF088260 Genomic DNA Translation: AAD39703.1 AF088261 Genomic DNA Translation: AAD39704.1 AF088262 Genomic DNA Translation: AAD39705.1 AF088263 Genomic DNA Translation: AAD39706.1 AF088264 Genomic DNA Translation: AAD39707.1 AF088265 Genomic DNA Translation: AAD39708.1 AF088266 Genomic DNA Translation: AAD39709.1 AF088267 Genomic DNA Translation: AAD39710.1 AF088268 Genomic DNA Translation: AAD39711.1 AF088269 Genomic DNA Translation: AAD39712.1 AF088270 Genomic DNA Translation: AAD39713.1 AF088271 Genomic DNA Translation: AAD39714.1 AF088272 Genomic DNA Translation: AAD39715.1 AF088273 Genomic DNA Translation: AAD39716.1 AF088274 Genomic DNA Translation: AAD39717.1 |
PIRi | A41585 A42770 |
RefSeqi | NP_001285948.1, NM_001299019.1 NP_476868.1, NM_057520.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5E24 | X-ray | 2.14 | E/F | 99-522 | [»] | |
SMRi | P28159 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 60899, 85 interactors |
DIPi | DIP-177N |
IntActi | P28159, 22 interactors |
MINTi | P28159 |
STRINGi | 7227.FBpp0080261 |
PTM databases
iPTMneti | P28159 |
Proteomic databases
PaxDbi | P28159 |
Genome annotation databases
EnsemblMetazoai | FBtr0080700; FBpp0080261; FBgn0004837 FBtr0346621; FBpp0312201; FBgn0004837 |
GeneIDi | 34881 |
KEGGi | dme:Dmel_CG3497 |
Organism-specific databases
CTDi | 34881 |
FlyBasei | FBgn0004837, Su(H) |
Phylogenomic databases
eggNOGi | KOG3743, Eukaryota |
HOGENOMi | CLU_022207_2_1_1 |
InParanoidi | P28159 |
OMAi | DMPHFGL |
OrthoDBi | 444988at2759 |
PhylomeDBi | P28159 |
Enzyme and pathway databases
Reactomei | R-DME-350054, Notch-HLH transcription pathway |
SignaLinki | P28159 |
Miscellaneous databases
BioGRID-ORCSi | 34881, 0 hits in 3 CRISPR screens |
GenomeRNAii | 34881 |
PROi | PR:P28159 |
Gene expression databases
Bgeei | FBgn0004837, Expressed in second segment of antenna (Drosophila) and 68 other tissues |
ExpressionAtlasi | P28159, baseline and differential |
Genevisiblei | P28159, DM |
Family and domain databases
CDDi | cd01176, IPT_RBP-Jkappa, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 2.60.40.1450, 1 hit |
InterProi | View protein in InterPro IPR015350, Beta-trefoil_DNA-bd_dom IPR036358, BTD_sf IPR040159, CLS_fam IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR008967, p53-like_TF_DNA-bd IPR015351, RBP-J/Cbf11/Cbf12_DNA-bd IPR037095, RBP-J/Cbf11/Cbf12_DNA-bd_sf IPR038007, RBP-Jkappa_IPT |
PANTHERi | PTHR10665, PTHR10665, 1 hit |
Pfami | View protein in Pfam PF09270, BTD, 1 hit PF09271, LAG1-DNAbind, 1 hit |
SMARTi | View protein in SMART SM01268, BTD, 1 hit SM01267, LAG1_DNAbind, 1 hit |
SUPFAMi | SSF110217, SSF110217, 1 hit SSF49417, SSF49417, 1 hit SSF81296, SSF81296, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SUH_DROME | |
Accessioni | P28159Primary (citable) accession number: P28159 Secondary accession number(s): Q3S1M8 Q9V446 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | April 7, 2021 | |
This is version 199 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families