UniProtKB - P28073 (PSB6_RAT)
Protein
Proteasome subunit beta type-6
Gene
Psmb6
Organism
Rattus norvegicus (Rat)
Status
Functioni
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB6 displays a peptidylglutamyl-hydrolyzing activity also termed postacidic or caspase-like activity, meaning that the peptides bond hydrolysis occurs directly after acidic residues.By similarity
Catalytic activityi
- Cleavage of peptide bonds with very broad specificity.By similarity EC:3.4.25.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 34 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- endopeptidase activity Source: GO_Central
- threonine-type endopeptidase activity Source: UniProtKB-KW
GO - Biological processi
- proteasomal protein catabolic process Source: GO_Central
- proteasomal ubiquitin-independent protein catabolic process Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Threonine protease |
Enzyme and pathway databases
Reactomei | R-RNO-1169091, Activation of NF-kappaB in B cells R-RNO-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-RNO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-RNO-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-RNO-174154, APC/C:Cdc20 mediated degradation of Securin R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-187577, SCF(Skp2)-mediated degradation of p27/p21 R-RNO-195253, Degradation of beta-catenin by the destruction complex R-RNO-202424, Downstream TCR signaling R-RNO-2467813, Separation of Sister Chromatids R-RNO-2871837, FCERI mediated NF-kB activation R-RNO-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-RNO-350562, Regulation of ornithine decarboxylase (ODC) R-RNO-382556, ABC-family proteins mediated transport R-RNO-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-RNO-4608870, Asymmetric localization of PCP proteins R-RNO-4641257, Degradation of AXIN R-RNO-4641258, Degradation of DVL R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-RNO-5607764, CLEC7A (Dectin-1) signaling R-RNO-5610780, Degradation of GLI1 by the proteasome R-RNO-5610785, GLI3 is processed to GLI3R by the proteasome R-RNO-5632684, Hedgehog 'on' state R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5668541, TNFR2 non-canonical NF-kB pathway R-RNO-5676590, NIK-->noncanonical NF-kB signaling R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689603, UCH proteinases R-RNO-5689880, Ub-specific processing proteases R-RNO-68827, CDT1 association with the CDC6:ORC:origin complex R-RNO-68949, Orc1 removal from chromatin R-RNO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-RNO-69481, G2/M Checkpoints R-RNO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-RNO-75815, Ubiquitin-dependent degradation of Cyclin D R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-8939902, Regulation of RUNX2 expression and activity R-RNO-8941858, Regulation of RUNX3 expression and activity R-RNO-8948751, Regulation of PTEN stability and activity R-RNO-8951664, Neddylation R-RNO-9020702, Interleukin-1 signaling R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
MEROPSi | T01.010 |
Names & Taxonomyi
Protein namesi | Recommended name: Proteasome subunit beta type-6 (EC:3.4.25.1By similarity)Alternative name(s): Macropain delta chain Multicatalytic endopeptidase complex delta chain Proteasome chain 5 Proteasome delta chain Proteasome subunit Y |
Gene namesi | Name:Psmb6 Synonyms:Psmb6l |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61881, Psmb6 |
Subcellular locationi
Nucleus
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- proteasome complex Source: RGD
- proteasome core complex Source: UniProtKB
- proteasome core complex, beta-subunit complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
PropeptideiPRO_0000026617 | 2 – 33 | Removed in mature form1 PublicationAdd BLAST | 32 | |
ChainiPRO_0000026618 | 34 – 238 | Proteasome subunit beta type-6Add BLAST | 205 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 68 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, Phosphoprotein, ZymogenProteomic databases
jPOSTi | P28073 |
PaxDbi | P28073 |
PRIDEi | P28073 |
2D gel databases
World-2DPAGEi | 0004:P28073 |
PTM databases
PhosphoSitePlusi | P28073 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000019551, Expressed in quadriceps femoris and 18 other tissues |
Genevisiblei | P28073, RN |
Interactioni
Subunit structurei
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.
By similarityProtein-protein interaction databases
BioGRIDi | 248287, 3 interactors |
IntActi | P28073, 1 interactor |
STRINGi | 10116.ENSRNOP00000026507 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28073 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase T1B family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0174, Eukaryota |
GeneTreei | ENSGT00940000155114 |
HOGENOMi | CLU_035750_5_1_1 |
InParanoidi | P28073 |
OMAi | DWINAPH |
OrthoDBi | 1172133at2759 |
PhylomeDBi | P28073 |
TreeFami | TF106221 |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR000243, Pept_T1A_subB IPR035140, Proteasome_beta6 IPR016050, Proteasome_bsu_CS IPR001353, Proteasome_sua/b IPR023333, Proteasome_suB-type |
PANTHERi | PTHR11599:SF46, PTHR11599:SF46, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit |
PRINTSi | PR00141, PROTEASOME |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00854, PROTEASOME_BETA_1, 1 hit PS51476, PROTEASOME_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P28073-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAALAVRGA VSAPAFGPEA LTPDWENREV STGTTIMAVQ FDGGVVLGAD
60 70 80 90 100
SRTTTGSYIA NRVTDKLTPI HDHIFCCRSG SAADTQAVAD AVTYQLGFHS
110 120 130 140 150
IELNEPPLVH TAASLFKEMC YRYREDLMAG IIIAGWDPQE GGQVYSVPMG
160 170 180 190 200
GMMVRQSFAI GGSGSSYIYG YVDATYREGM TKDECLQFTA NALALAMERD
210 220 230
GSSGGVIRLA AIQQSGVERQ VLLGDQIPKV TISTLPPP
Sequence cautioni
The sequence BAA01586 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC058451 mRNA Translation: AAH58451.1 AABR03076595 Genomic DNA No translation available. D10754 mRNA Translation: BAA01586.1 Different initiation. BN000325 mRNA Translation: CAE48380.1 |
PIRi | JX0228 S09086 |
RefSeqi | NP_001316812.1, NM_001329883.1 NP_476440.2, NM_057099.3 |
Genome annotation databases
Ensembli | ENSRNOT00000026507; ENSRNOP00000026507; ENSRNOG00000019551 |
GeneIDi | 100360846 29666 |
KEGGi | rno:100360846 rno:29666 |
UCSCi | RGD:61881, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC058451 mRNA Translation: AAH58451.1 AABR03076595 Genomic DNA No translation available. D10754 mRNA Translation: BAA01586.1 Different initiation. BN000325 mRNA Translation: CAE48380.1 |
PIRi | JX0228 S09086 |
RefSeqi | NP_001316812.1, NM_001329883.1 NP_476440.2, NM_057099.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6EPC | electron microscopy | 12.30 | 1 | 1-238 | [»] | |
6EPD | electron microscopy | 15.40 | 1 | 1-238 | [»] | |
6EPE | electron microscopy | 12.80 | 1 | 1-238 | [»] | |
6EPF | electron microscopy | 11.80 | 1 | 1-238 | [»] | |
6TU3 | electron microscopy | 2.70 | H/V | 1-238 | [»] | |
SMRi | P28073 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248287, 3 interactors |
IntActi | P28073, 1 interactor |
STRINGi | 10116.ENSRNOP00000026507 |
Protein family/group databases
MEROPSi | T01.010 |
PTM databases
PhosphoSitePlusi | P28073 |
2D gel databases
World-2DPAGEi | 0004:P28073 |
Proteomic databases
jPOSTi | P28073 |
PaxDbi | P28073 |
PRIDEi | P28073 |
Genome annotation databases
Ensembli | ENSRNOT00000026507; ENSRNOP00000026507; ENSRNOG00000019551 |
GeneIDi | 100360846 29666 |
KEGGi | rno:100360846 rno:29666 |
UCSCi | RGD:61881, rat |
Organism-specific databases
CTDi | 5694 |
RGDi | 61881, Psmb6 |
Phylogenomic databases
eggNOGi | KOG0174, Eukaryota |
GeneTreei | ENSGT00940000155114 |
HOGENOMi | CLU_035750_5_1_1 |
InParanoidi | P28073 |
OMAi | DWINAPH |
OrthoDBi | 1172133at2759 |
PhylomeDBi | P28073 |
TreeFami | TF106221 |
Enzyme and pathway databases
Reactomei | R-RNO-1169091, Activation of NF-kappaB in B cells R-RNO-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-RNO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-RNO-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-RNO-174154, APC/C:Cdc20 mediated degradation of Securin R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-187577, SCF(Skp2)-mediated degradation of p27/p21 R-RNO-195253, Degradation of beta-catenin by the destruction complex R-RNO-202424, Downstream TCR signaling R-RNO-2467813, Separation of Sister Chromatids R-RNO-2871837, FCERI mediated NF-kB activation R-RNO-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-RNO-350562, Regulation of ornithine decarboxylase (ODC) R-RNO-382556, ABC-family proteins mediated transport R-RNO-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-RNO-4608870, Asymmetric localization of PCP proteins R-RNO-4641257, Degradation of AXIN R-RNO-4641258, Degradation of DVL R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-RNO-5607764, CLEC7A (Dectin-1) signaling R-RNO-5610780, Degradation of GLI1 by the proteasome R-RNO-5610785, GLI3 is processed to GLI3R by the proteasome R-RNO-5632684, Hedgehog 'on' state R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5668541, TNFR2 non-canonical NF-kB pathway R-RNO-5676590, NIK-->noncanonical NF-kB signaling R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689603, UCH proteinases R-RNO-5689880, Ub-specific processing proteases R-RNO-68827, CDT1 association with the CDC6:ORC:origin complex R-RNO-68949, Orc1 removal from chromatin R-RNO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-RNO-69481, G2/M Checkpoints R-RNO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-RNO-75815, Ubiquitin-dependent degradation of Cyclin D R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-8939902, Regulation of RUNX2 expression and activity R-RNO-8941858, Regulation of RUNX3 expression and activity R-RNO-8948751, Regulation of PTEN stability and activity R-RNO-8951664, Neddylation R-RNO-9020702, Interleukin-1 signaling R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:P28073 |
Gene expression databases
Bgeei | ENSRNOG00000019551, Expressed in quadriceps femoris and 18 other tissues |
Genevisiblei | P28073, RN |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR000243, Pept_T1A_subB IPR035140, Proteasome_beta6 IPR016050, Proteasome_bsu_CS IPR001353, Proteasome_sua/b IPR023333, Proteasome_suB-type |
PANTHERi | PTHR11599:SF46, PTHR11599:SF46, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit |
PRINTSi | PR00141, PROTEASOME |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00854, PROTEASOME_BETA_1, 1 hit PS51476, PROTEASOME_BETA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSB6_RAT | |
Accessioni | P28073Primary (citable) accession number: P28073 Secondary accession number(s): Q6IE68, Q6PDW5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families