UniProtKB - P28067 (DMA_HUMAN)
Protein
HLA class II histocompatibility antigen, DM alpha chain
Gene
HLA-DMA
Organism
Homo sapiens (Human)
Status
Functioni
Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.4 Publications
GO - Molecular functioni
- MHC class II protein complex binding Source: UniProtKB
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- peptide antigen assembly with MHC class II protein complex Source: UniProtKB
Keywordsi
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
PathwayCommonsi | P28067 |
Reactomei | R-HSA-2132295, MHC class II antigen presentation |
SIGNORi | P28067 |
Names & Taxonomyi
Protein namesi | Recommended name: HLA class II histocompatibility antigen, DM alpha chainAlternative name(s): MHC class II antigen DMA Really interesting new gene 6 protein |
Gene namesi | Name:HLA-DMA Synonyms:DMA, RING6 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4934, HLA-DMA |
MIMi | 142855, gene |
neXtProti | NX_P28067 |
VEuPathDBi | HostDB:ENSG00000204257.14 |
Subcellular locationi
Lysosome
Endosome
Note: Localizes to late endocytic compartment. Associates with lysosome membranes.
Endosome
- late endosome membrane Source: UniProtKB-SubCell
Lysosome
- lysosomal membrane Source: Reactome
Plasma Membrane
- MHC class II protein complex Source: UniProtKB
Other locations
- cell surface Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: HPA
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 27 – 233 | LumenalSequence analysisAdd BLAST | 207 | |
Transmembranei | 234 – 254 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 255 – 261 | CytoplasmicSequence analysis | 7 |
Keywords - Cellular componenti
Endosome, Lysosome, Membrane, MHC IIPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 124 | R → A: Decreases the interaction with MHCII and peptide exchange; when associated with A-220. 1 Publication | 1 | |
Mutagenesisi | 126 | F → A: Decreases the interaction with MHCII and peptide exchange. 1 Publication | 1 | |
Mutagenesisi | 151 | N → A or R: Abrogates the interaction with MHCII and peptide exchange. 1 Publication | 1 | |
Mutagenesisi | 199 | I → N: Decreases the interaction with MHCII and peptide exchange. 1 Publication | 1 | |
Mutagenesisi | 220 | R → A: Decreases the interaction with MHCII and peptide exchange; when associated with A-124. 1 Publication | 1 |
Miscellaneous databases
Pharosi | P28067, Tbio |
Genetic variation databases
BioMutai | HLA-DMA |
DMDMi | 133158 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000018958 | 27 – 261 | HLA class II histocompatibility antigen, DM alpha chainAdd BLAST | 235 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 41 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Disulfide bondi | 50 ↔ 105 | Combined sources1 Publication | ||
Disulfide bondi | 147 ↔ 202 | Combined sources1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | P28067 |
jPOSTi | P28067 |
MassIVEi | P28067 |
MaxQBi | P28067 |
PaxDbi | P28067 |
PeptideAtlasi | P28067 |
PRIDEi | P28067 |
ProteomicsDBi | 54442 |
PTM databases
GlyGeni | P28067, 1 site |
iPTMneti | P28067 |
PhosphoSitePlusi | P28067 |
Expressioni
Gene expression databases
Bgeei | ENSG00000204257, Expressed in lymph node and 220 other tissues |
ExpressionAtlasi | P28067, baseline and differential |
Genevisiblei | P28067, HS |
Organism-specific databases
HPAi | ENSG00000204257, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
P28067
With | #Exp. | IntAct |
---|---|---|
HLA-DMB [P28068] | 22 | EBI-3865396,EBI-2877138 |
Protein-protein interaction databases
DIPi | DIP-6184N |
IntActi | P28067, 10 interactors |
MINTi | P28067 |
STRINGi | 9606.ENSP00000363976 |
Miscellaneous databases
RNActi | P28067, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28067 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28067 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 121 – 215 | Ig-like C1-typeAdd BLAST | 95 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 27 – 124 | Alpha-1Add BLAST | 98 | |
Regioni | 125 – 217 | Alpha-2Add BLAST | 93 | |
Regioni | 218 – 233 | Connecting peptideSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Belongs to the MHC class II family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S6RX, Eukaryota |
HOGENOMi | CLU_069380_1_0_1 |
InParanoidi | P28067 |
PhylomeDBi | P28067 |
TreeFami | TF333797 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit 3.10.320.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003006, Ig/MHC_CS IPR003597, Ig_C1-set IPR011162, MHC_I/II-like_Ag-recog IPR014745, MHC_II_a/b_N IPR001003, MHC_II_a_N |
Pfami | View protein in Pfam PF07654, C1-set, 1 hit PF00993, MHC_II_alpha, 1 hit |
SMARTi | View protein in SMART SM00407, IGc1, 1 hit SM00920, MHC_II_alpha, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF54452, SSF54452, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit PS00290, IG_MHC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 21 potential isoforms that are computationally mapped.Show allAlign All
P28067-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGHEQNQGAA LLQMLPLLWL LPHSWAVPEA PTPMWPDDLQ NHTFLHTVYC
60 70 80 90 100
QDGSPSVGLS EAYDEDQLFF FDFSQNTRVP RLPEFADWAQ EQGDAPAILF
110 120 130 140 150
DKEFCEWMIQ QIGPKLDGKI PVSRGFPIAE VFTLKPLEFG KPNTLVCFVS
160 170 180 190 200
NLFPPMLTVN WHDHSVPVEG FGPTFVSAVD GLSFQAFSYL NFTPEPSDIF
210 220 230 240 250
SCIVTHEIDR YTAIAYWVPR NALPSDLLEN VLCGVAFGLG VLGIIVGIVL
260
IIYFRKPCSG D
Computationally mapped potential isoform sequencesi
There are 21 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y732 | H0Y732_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 290 | Annotation score: | ||
Q5SP00 | Q5SP00_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 227 | Annotation score: | ||
A0A140T9G2 | A0A140T9G2_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 227 | Annotation score: | ||
A0A140T9H8 | A0A140T9H8_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 261 | Annotation score: | ||
A0A140T9K8 | A0A140T9K8_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 290 | Annotation score: | ||
A0A140T9N9 | A0A140T9N9_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 290 | Annotation score: | ||
A0A140T9T3 | A0A140T9T3_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 290 | Annotation score: | ||
A0A140T9V7 | A0A140T9V7_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 290 | Annotation score: | ||
A0A140T9X4 | A0A140T9X4_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 290 | Annotation score: | ||
A0A140T9N7 | A0A140T9N7_HUMAN | HLA class II histocompatibility ant... | HLA-DMA | 290 | Annotation score: | ||
There are more potential isoformsShow all |
Polymorphismi
The following alleles of DMA are known: DMA*01:01, DMA*01:02, DMA*01:03 (DMA3.2) and DMA*01:04 (DMA3.4). The sequence shown is that of DMA*01:01.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_016746 | 162 | H → Q in allele DMA*01:03 and allele DMA*01:04. | 1 | |
Natural variantiVAR_016747 | 163 | D → H in allele DMA*01:03 and allele DMA*01:04. | 1 | |
Natural variantiVAR_016748 | 166 | V → I in allele DMA*01:02 and allele DMA*01:04. Corresponds to variant dbSNP:rs1063478Ensembl. | 1 | |
Natural variantiVAR_016749 | 181 | G → A in allele DMA*01:03. Corresponds to variant dbSNP:rs6926628Ensembl. | 1 | |
Natural variantiVAR_016750 | 210 | R → C in allele DMA*01:04. Corresponds to variant dbSNP:rs17214044Ensembl. | 1 | |
Natural variantiVAR_016751 | 210 | R → H in allele DMA*01:03. Corresponds to variant dbSNP:rs41555121Ensembl. | 1 | |
Natural variantiVAR_056544 | 235 | V → M. Corresponds to variant dbSNP:rs9469319Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62744 mRNA Translation: CAA44606.1 AL935042 Genomic DNA No translation available. Z24753 Genomic DNA No translation available. U04878 Genomic DNA Translation: AAA56994.1 U04877 Genomic DNA Translation: AAA56993.1 |
CCDSi | CCDS4761.1 |
PIRi | I38490 S17886 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62744 mRNA Translation: CAA44606.1 AL935042 Genomic DNA No translation available. Z24753 Genomic DNA No translation available. U04878 Genomic DNA Translation: AAA56994.1 U04877 Genomic DNA Translation: AAA56993.1 |
CCDSi | CCDS4761.1 |
PIRi | I38490 S17886 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HDM | X-ray | 2.50 | A | 27-230 | [»] | |
2BC4 | X-ray | 2.27 | A/C | 27-229 | [»] | |
4FQX | X-ray | 2.60 | C | 27-225 | [»] | |
4GBX | X-ray | 3.00 | C | 27-225 | [»] | |
SMRi | P28067 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-6184N |
IntActi | P28067, 10 interactors |
MINTi | P28067 |
STRINGi | 9606.ENSP00000363976 |
PTM databases
GlyGeni | P28067, 1 site |
iPTMneti | P28067 |
PhosphoSitePlusi | P28067 |
Genetic variation databases
BioMutai | HLA-DMA |
DMDMi | 133158 |
Proteomic databases
EPDi | P28067 |
jPOSTi | P28067 |
MassIVEi | P28067 |
MaxQBi | P28067 |
PaxDbi | P28067 |
PeptideAtlasi | P28067 |
PRIDEi | P28067 |
ProteomicsDBi | 54442 |
Protocols and materials databases
Antibodypediai | 2402, 373 antibodies |
Genome annotation databases
Organism-specific databases
GeneCardsi | HLA-DMA |
HGNCi | HGNC:4934, HLA-DMA |
HPAi | ENSG00000204257, Low tissue specificity |
MIMi | 142855, gene |
neXtProti | NX_P28067 |
VEuPathDBi | HostDB:ENSG00000204257.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S6RX, Eukaryota |
HOGENOMi | CLU_069380_1_0_1 |
InParanoidi | P28067 |
PhylomeDBi | P28067 |
TreeFami | TF333797 |
Enzyme and pathway databases
PathwayCommonsi | P28067 |
Reactomei | R-HSA-2132295, MHC class II antigen presentation |
SIGNORi | P28067 |
Miscellaneous databases
ChiTaRSi | HLA-DMA, human |
EvolutionaryTracei | P28067 |
Pharosi | P28067, Tbio |
PROi | PR:P28067 |
RNActi | P28067, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204257, Expressed in lymph node and 220 other tissues |
ExpressionAtlasi | P28067, baseline and differential |
Genevisiblei | P28067, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit 3.10.320.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003006, Ig/MHC_CS IPR003597, Ig_C1-set IPR011162, MHC_I/II-like_Ag-recog IPR014745, MHC_II_a/b_N IPR001003, MHC_II_a_N |
Pfami | View protein in Pfam PF07654, C1-set, 1 hit PF00993, MHC_II_alpha, 1 hit |
SMARTi | View protein in SMART SM00407, IGc1, 1 hit SM00920, MHC_II_alpha, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF54452, SSF54452, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit PS00290, IG_MHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DMA_HUMAN | |
Accessioni | P28067Primary (citable) accession number: P28067 Secondary accession number(s): Q29639, Q29640 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | February 10, 2021 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families