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Protein

Proteasome subunit alpha type-5

Gene

PSMA5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).3 Publications

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-6798695 Neutrophil degranulation
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SIGNORiP28066

Protein family/group databases

MEROPSiT01.975

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-5 (EC:3.4.25.11 Publication)
Alternative name(s):
Macropain zeta chain
Multicatalytic endopeptidase complex zeta chain
Proteasome zeta chain
Gene namesi
Name:PSMA5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143106.12
HGNCiHGNC:9534 PSMA5
MIMi176844 gene
neXtProtiNX_P28066

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5686
OpenTargetsiENSG00000143106
PharmGKBiPA33879

Chemistry databases

ChEMBLiCHEMBL3831201

Polymorphism and mutation databases

BioMutaiPSMA5
DMDMi38258905

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241171 – 241Proteasome subunit alpha type-5Add BLAST241

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei16PhosphoserineCombined sources1
Modified residuei55PhosphothreonineCombined sources1
Modified residuei56PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Glycosylationi198O-linked (GlcNAc) serineBy similarity1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP28066
MaxQBiP28066
PaxDbiP28066
PeptideAtlasiP28066
PRIDEiP28066
ProteomicsDBi54441
TopDownProteomicsiP28066-1 [P28066-1]

2D gel databases

DOSAC-COBS-2DPAGEiP28066
REPRODUCTION-2DPAGEiIPI00291922
SWISS-2DPAGEiP28066

PTM databases

iPTMnetiP28066
PhosphoSitePlusiP28066
SwissPalmiP28066

Expressioni

Tissue specificityi

Expressed in fetal brain (at protein level).1 Publication

Inductioni

Up-regulated in colon cancer cell lines. Up-regulated in fetal Down syndrome (DS) brain (at protein level). May be the target of the transcriptional activator NFE2L2.2 Publications

Gene expression databases

BgeeiENSG00000143106
CleanExiHS_PSMA5
ExpressionAtlasiP28066 baseline and differential
GenevisibleiP28066 HS

Organism-specific databases

HPAiHPA028392
HPA028398
HPA028441

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi111659, 145 interactors
CORUMiP28066
DIPiDIP-29368N
IntActiP28066, 34 interactors
MINTiP28066
STRINGi9606.ENSP00000271308

Chemistry databases

BindingDBiP28066

Structurei

Secondary structure

1241
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 21Combined sources3
Helixi22 – 31Combined sources10
Beta strandi37 – 42Combined sources6
Beta strandi45 – 51Combined sources7
Beta strandi57 – 59Combined sources3
Helixi61 – 63Combined sources3
Beta strandi67 – 71Combined sources5
Beta strandi74 – 80Combined sources7
Helixi82 – 84Combined sources3
Helixi85 – 103Combined sources19
Helixi109 – 117Combined sources9
Turni118 – 121Combined sources4
Beta strandi125 – 128Combined sources4
Beta strandi133 – 135Combined sources3
Beta strandi139 – 147Combined sources9
Beta strandi150 – 156Combined sources7
Beta strandi162 – 171Combined sources10
Helixi174 – 184Combined sources11
Helixi191 – 205Combined sources15
Beta strandi206 – 208Combined sources3
Beta strandi214 – 220Combined sources7
Beta strandi222 – 224Combined sources3
Helixi231 – 238Combined sources8

3D structure databases

ProteinModelPortaliP28066
SMRiP28066
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0176 Eukaryota
COG0638 LUCA
GeneTreeiENSGT00550000074958
HOGENOMiHOG000091085
HOVERGENiHBG003005
InParanoidiP28066
KOiK02729
OMAiFQVEYAR
OrthoDBiEOG091G0GX6
PhylomeDBiP28066
TreeFamiTF106211

Family and domain databases

CDDicd03753 proteasome_alpha_type_5, 1 hit
Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR033812 Proteasome_alpha_type_5
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28066-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFLTRSEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGI QTSEGVCLAV
60 70 80 90 100
EKRITSPLME PSSIEKIVEI DAHIGCAMSG LIADAKTLID KARVETQNHW
110 120 130 140 150
FTYNETMTVE SVTQAVSNLA LQFGEEDADP GAMSRPFGVA LLFGGVDEKG
160 170 180 190 200
PQLFHMDPSG TFVQCDARAI GSASEGAQSS LQEVYHKSMT LKEAIKSSLI
210 220 230 240
ILKQVMEEKL NATNIELATV QPGQNFHMFT KEELEEVIKD I
Length:241
Mass (Da):26,411
Last modified:October 31, 2003 - v3
Checksum:i5610CDA00469120A
GO
Isoform 2 (identifier: P28066-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.

Note: No experimental confirmation available.
Show »
Length:183
Mass (Da):20,035
Checksum:i24D3C1197D3AD8A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27A → D in CAA43962 (PubMed:1888762).Curated1
Sequence conflicti184V → L in CAA43962 (PubMed:1888762).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0462411 – 58Missing in isoform 2. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61970 mRNA Translation: CAA43962.1
CR456847 mRNA Translation: CAG33128.1
AK304448 mRNA Translation: BAG65266.1
AK313351 mRNA Translation: BAG36153.1
AL356735 Genomic DNA No translation available.
AL390252 Genomic DNA No translation available.
CH471122 Genomic DNA Translation: EAW56381.1
CH471122 Genomic DNA Translation: EAW56383.1
BC102018 mRNA Translation: AAI02019.1
BC102019 mRNA Translation: AAI02020.1
BC102020 mRNA Translation: AAI02021.1
BC103751 mRNA Translation: AAI03752.1
CCDSiCCDS55619.1 [P28066-2]
CCDS799.1 [P28066-1]
PIRiS17521
RefSeqiNP_001186701.1, NM_001199772.1 [P28066-2]
NP_001186702.1, NM_001199773.1 [P28066-2]
NP_001186703.1, NM_001199774.1 [P28066-2]
NP_002781.2, NM_002790.3 [P28066-1]
UniGeneiHs.485246

Genome annotation databases

EnsembliENST00000271308; ENSP00000271308; ENSG00000143106 [P28066-1]
ENST00000538610; ENSP00000440618; ENSG00000143106 [P28066-2]
GeneIDi5686
KEGGihsa:5686
UCSCiuc001dxn.4 human [P28066-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSA5_HUMAN
AccessioniPrimary (citable) accession number: P28066
Secondary accession number(s): B2R8F6
, B4E2V4, Q3T1C1, Q6IBF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 31, 2003
Last modified: July 18, 2018
This is version 206 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

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