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Protein

Proteasome subunit beta

Gene

psmB

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The T.acidophilum proteasome is able to cleave oligopeptides after Tyr, Leu, Phe, and to a lesser extent after Glu and Arg. Thus, displays chymotrypsin-like activity and low level of caspase-like and trypsin-like activities.UniRule annotation1 Publication

Miscellaneous

The configuration of the closed gate contains an opening large enough to allow rapid entry of tetrapeptides but able to impede the entry of proteins and longer peptides.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation1 Publication

Activity regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation2 Publications

Kineticsi

  1. KM=39 µM for Suc-LLVY-Amc (at 55 degrees Celsius)1 Publication
  1. Vmax=28 nmol/min/mg enzyme with Suc-LLVY-Amc as substrate (at 55 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9NucleophileUniRule annotation1 Publication1

GO - Molecular functioni

  • endopeptidase activity Source: UniProtKB
  • threonine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciTACI273075:G1GT7-642-MONOMER

Protein family/group databases

MEROPSiT01.002

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome beta subunitUniRule annotation
Proteasome core protein PsmBUniRule annotation
Gene namesi
Name:psmBUniRule annotation
Ordered Locus Names:Ta0612
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9T → S: No effect on catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000266751 – 8Removed in mature form; by autocatalysisUniRule annotation1 Publication8
ChainiPRO_00000266769 – 211Proteasome subunit betaAdd BLAST203

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Proteomic databases

PRIDEiP28061

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.UniRule annotation6 Publications

Protein-protein interaction databases

STRINGi273075.Ta0612

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP28061
SMRiP28061
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28061

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00970 Archaea
COG0638 LUCA
HOGENOMiHOG000091083
KOiK03433
OMAiDLCVIRK
OrthoDBiPOG093Z0CM8

Family and domain databases

CDDicd03764 proteasome_beta_archeal, 1 hit
Gene3Di3.60.20.10, 1 hit
HAMAPiMF_02113_A Proteasome_B_A, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR019983 Pept_T1A_Psome_bsu_arc
IPR000243 Pept_T1A_subB
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PANTHERiPTHR11599:SF108 PTHR11599:SF108, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PRINTSiPR00141 PROTEASOME
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR03634 arc_protsome_B, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28061-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNQTLETGTT TVGITLKDAV IMATERRVTM ENFIMHKNGK KLFQIDTYTG
60 70 80 90 100
MTIAGLVGDA QVLVRYMKAE LELYRLQRRV NMPIEAVATL LSNMLNQVKY
110 120 130 140 150
MPYMVQLLVG GIDTAPHVFS IDAAGGSVED IYASTGSGSP FVYGVLESQY
160 170 180 190 200
SEKMTVDEGV DLVIRAISAA KQRDSASGGM IDVAVITRKD GYVQLPTDQI
210
ESRIRKLGLI L
Length:211
Mass (Da):23,147
Last modified:August 1, 1992 - v1
Checksum:iF81B9360641873DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83674 Genomic DNA Translation: AAA72102.1
AL445064 Genomic DNA Translation: CAC11751.1
PIRiA42068
RefSeqiWP_010901036.1, NC_002578.1

Genome annotation databases

EnsemblBacteriaiCAC11751; CAC11751; CAC11751
GeneIDi1456195
KEGGitac:Ta0612

Similar proteinsi

Entry informationi

Entry nameiPSB_THEAC
AccessioniPrimary (citable) accession number: P28061
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 12, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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