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Protein

Protein Wnt-8

Gene

wnt8

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in ventral mesodermal patterning during embryogenesis. Mimics Nieuwkoop center activity. Causes dorsal mesodermal differentiation of animal cap ectoderm when coexpressed with noggin and nuclear, sequence-specific DNA-binding protein xBra. None of these molecules causes dorsal mesoderm formation when expressed alone.1 Publication

GO - Molecular functioni

  • frizzled binding Source: ParkinsonsUK-UCL
  • protease binding Source: ParkinsonsUK-UCL

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-8
Short name:
XWnt-8
Gene namesi
Name:wnt8
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-866281 wnt8a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000004144723 – 358Protein Wnt-8Add BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 661 Publication
Glycosylationi104N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi105 ↔ 1131 Publication
Disulfide bondi115 ↔ 1331 Publication
Disulfide bondi181 ↔ 1951 Publication
Disulfide bondi183 ↔ 1901 Publication
Lipidationi187O-palmitoleoyl serine1 Publication1
Disulfide bondi260 ↔ 2981 Publication
Glycosylationi263N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi276 ↔ 2911 Publication
Glycosylationi282N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi295 ↔ 3371 Publication
Disulfide bondi313 ↔ 3281 Publication
Disulfide bondi315 ↔ 3251 Publication
Disulfide bondi320 ↔ 3211 Publication

Post-translational modificationi

Palmitoleoylation is required for efficient binding to frizzled receptors (PubMed:22653731). Depalmitoleoylation leads to Wnt signaling pathway inhibition (By similarity).By similarity1 Publication
Proteolytic processing by tiki1 and tiki2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of wnt8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei39 – 40Cleavage; by tiki1 and tiki21 Publication2
Sitei42 – 43Cleavage; by tiki1 and tiki21 Publication2

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

PTM databases

iPTMnetiP28026

Expressioni

Developmental stagei

Mid-blastula, decline by tailbud.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked, leading to inactivation. Interacts with the long chain of cer1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ptk7Q6PA072EBI-6257743,EBI-7036323

GO - Molecular functioni

Protein-protein interaction databases

BioGridi99305, 1 interactor
IntActiP28026, 2 interactors
MINTiP28026

Structurei

Secondary structure

1358
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiP28026
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG001595
KOiK00714

Family and domain databases

InterProiView protein in InterPro
IPR034312 Protein_Wnt-8A/8C
IPR005817 Wnt
IPR013301 Wnt8
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PTHR12027:SF92 PTHR12027:SF92, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01892 WNT8PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28026-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQNTTLFILA TLLIFCPFFT ASAWSVNNFL MTGPKAYLTY SASVAVGAQN
60 70 80 90 100
GIEECKYQFA WERWNCPEST LQLATHNGLR SATRETSFVH AISSAGVMYT
110 120 130 140 150
LTRNCSMGDF DNCGCDDSRN GRIGGRGWVW GGCSDNAEFG ERISKLFVDG
160 170 180 190 200
LETGQDARAL MNLHNNEAGR LAVKETMKRT CKCHGISGSC SIQTCWLQLA
210 220 230 240 250
EFRDIGNHLK IKHDQALKLE MDKRKMRSGN SADNRGAIAD AFSSVAGSEL
260 270 280 290 300
IFLEDSPDYC LKNISLGLQG TEGRECLQSG KNLSQWERRS CKRLCTDCGL
310 320 330 340 350
RVEEKKTEII SSCNCKFHWC CTVKCEQCKQ VVIKHFCARR ERDSNMLNTK

RKNRGHRR
Length:358
Mass (Da):40,176
Last modified:October 1, 1996 - v2
Checksum:i8BC8B9E20016E504
GO

Sequence cautioni

The sequence AAA50012 differs from that shown.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243S → T in AAA50012 (PubMed:1991549).Curated1
Sequence conflicti292K → R in AAA50012 (PubMed:1991549).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57234 mRNA Translation: CAA40510.1
M55058 mRNA Translation: AAA50012.1 Sequence problems.
PIRiS18771
RefSeqiNP_001081637.1, NM_001088168.1
UniGeneiXl.49

Genome annotation databases

GeneIDi397970
KEGGixla:397970

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57234 mRNA Translation: CAA40510.1
M55058 mRNA Translation: AAA50012.1 Sequence problems.
PIRiS18771
RefSeqiNP_001081637.1, NM_001088168.1
UniGeneiXl.49

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F0AX-ray3.25B23-338[»]
SMRiP28026
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi99305, 1 interactor
IntActiP28026, 2 interactors
MINTiP28026

PTM databases

iPTMnetiP28026

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397970
KEGGixla:397970

Organism-specific databases

CTDi397970
XenbaseiXB-GENE-866281 wnt8a

Phylogenomic databases

HOVERGENiHBG001595
KOiK00714

Family and domain databases

InterProiView protein in InterPro
IPR034312 Protein_Wnt-8A/8C
IPR005817 Wnt
IPR013301 Wnt8
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PTHR12027:SF92 PTHR12027:SF92, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01892 WNT8PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWNT8_XENLA
AccessioniPrimary (citable) accession number: P28026
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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