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Protein

H/ACA ribonucleoprotein complex subunit GAR1

Gene

GAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic component of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP), which catalyzes pseudouridylation of rRNA and is required for ribosome biogenesis (PubMed:9303321, PubMed:9843512). This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1 (PubMed:9303321, PubMed:9843512). Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs (PubMed:9843512). The H/ACA snoRNP complex also mediates pseudouridylation of other types of RNAs (PubMed:21131909). The H/ACA snoRNP complex mediates pseudouridylation at position 93 in U2 snRNA (PubMed:21131909). Essential for growth (PubMed:9303321).2 Publications

Miscellaneous

Present with 5730 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • box H/ACA snoRNA binding Source: SGD

GO - Biological processi

  • snoRNA guided rRNA pseudouridine synthesis Source: GO_Central
  • snRNA pseudouridine synthesis Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-31136-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit GAR1
Alternative name(s):
snoRNP protein GAR1
Gene namesi
Name:GAR1
Ordered Locus Names:YHR089C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR089C
SGDiS000001131 GAR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085661 – 205H/ACA ribonucleoprotein complex subunit GAR1Add BLAST205

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4Asymmetric dimethylarginine1 Publication1
Modified residuei8Asymmetric dimethylarginine1 Publication1
Modified residuei11Asymmetric dimethylarginine1 Publication1
Modified residuei15Asymmetric dimethylarginine1 Publication1
Modified residuei19Asymmetric dimethylarginine1 Publication1
Cross-linki77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei147Asymmetric dimethylarginine1 Publication1
Modified residuei154Asymmetric dimethylarginine1 Publication1
Modified residuei158Asymmetric dimethylarginine1 Publication1
Modified residuei162Asymmetric dimethylarginine1 Publication1
Modified residuei165Asymmetric dimethylarginine1 Publication1
Modified residuei171Asymmetric dimethylarginine1 Publication1
Modified residuei174Asymmetric dimethylarginine1 Publication1
Modified residuei180Asymmetric dimethylarginine1 Publication1
Modified residuei184Asymmetric dimethylarginine1 Publication1
Modified residuei189Asymmetric dimethylarginine1 Publication1
Modified residuei193Asymmetric dimethylarginine1 Publication1
Modified residuei197Asymmetric dimethylarginine1 Publication1
Modified residuei201Asymmetric dimethylarginine1 Publication1

Post-translational modificationi

Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues.1 Publication

Keywords - PTMi

Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

MaxQBiP28007
PaxDbiP28007
PRIDEiP28007

PTM databases

iPTMnetiP28007

Interactioni

Subunit structurei

Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs) (PubMed:9556561, PubMed:9843512, PubMed:21131909). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10 (PubMed:9556561, PubMed:9843512, PubMed:21131909). The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind. Interacts with snoRNAs (PubMed:9556561, PubMed:9843512).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NHP2P324953EBI-7321,EBI-12014

Protein-protein interaction databases

BioGridi36524, 192 interactors
ComplexPortaliCPX-737 Box H/ACA ribonucleoprotein complex
DIPiDIP-4484N
IntActiP28007, 83 interactors
MINTiP28007
STRINGi4932.YHR089C

Structurei

Secondary structure

1205
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 47Combined sources11
Beta strandi50 – 55Combined sources6
Beta strandi57 – 61Combined sources5
Beta strandi66 – 68Combined sources3
Beta strandi74 – 84Combined sources11
Beta strandi89 – 94Combined sources6
Helixi100 – 102Combined sources3
Beta strandi108 – 112Combined sources5
Helixi113 – 115Combined sources3
Helixi119 – 122Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3U28X-ray1.90C32-124[»]
3UAIX-ray3.06C32-124[»]
ProteinModelPortaliP28007
SMRiP28007
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 21RGG-box 1Add BLAST18
Regioni147 – 205RGG-box 2Add BLAST59

Sequence similaritiesi

Belongs to the GAR1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00730000111223
InParanoidiP28007
KOiK11128
OMAiFQQRDMG
OrthoDBiEOG092C5OR3

Family and domain databases

Gene3Di2.40.10.230, 1 hit
InterProiView protein in InterPro
IPR038664 Gar1/Naf1_Cbf5-bd_sf
IPR021154 H/ACA_rnp_Gar1
IPR007504 H/ACA_rnp_Gar1/Naf1
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR23237 PTHR23237, 1 hit
PfamiView protein in Pfam
PF04410 Gar1, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD020235 Gar1_RNA_bd, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit

Sequencei

Sequence statusi: Complete.

P28007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRGGNRGG RGGFRGGFRG GRTGSARSFQ QGPPDTVLEM GAFLHPCEGD
60 70 80 90 100
IVCRSINTKI PYFNAPIYLE NKTQVGKVDE ILGPLNEVFF TIKCGDGVQA
110 120 130 140 150
TSFKEGDKFY IAADKLLPIE RFLPKPKVVG PPKPKNKKKR SGAPGGRGGA
160 170 180 190 200
SMGRGGSRGG FRGGRGGSSF RGGRGGSSFR GGSRGGSFRG GSRGGSRGGF

RGGRR
Length:205
Mass (Da):21,480
Last modified:August 1, 1992 - v1
Checksum:iC197FE85644DD0F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63617 Genomic DNA Translation: CAA45162.1
U00060 Genomic DNA Translation: AAB68929.1
AY558284 Genomic DNA Translation: AAS56610.1
BK006934 Genomic DNA Translation: DAA06785.1
PIRiS19634
RefSeqiNP_011957.1, NM_001179219.1

Genome annotation databases

EnsemblFungiiYHR089C; YHR089C; YHR089C
GeneIDi856489
KEGGisce:YHR089C

Similar proteinsi

Entry informationi

Entry nameiGAR1_YEAST
AccessioniPrimary (citable) accession number: P28007
Secondary accession number(s): D3DL41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 20, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

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