UniProtKB - P28004 (PRP45_YEAST)
Protein
Pre-mRNA-processing protein 45
Gene
PRP45
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in pre-mRNA splicing. Associated with the spliceosome throughout the splicing reactions, until after the second catalytic step.2 Publications
Miscellaneous
Present with 1670 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-processing protein 45 |
Gene namesi | Name:PRP45 Synonyms:FUN20 Ordered Locus Names:YAL032C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YAL032C |
SGDi | S000000030, PRP45 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Nucleus
- nucleus Source: SGD
- post-mRNA release spliceosomal complex Source: SGD
- U2-type catalytic step 1 spliceosome Source: SGD
- U2-type catalytic step 2 spliceosome Source: SGD
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084825 | 1 – 379 | Pre-mRNA-processing protein 45Add BLAST | 379 |
Proteomic databases
MaxQBi | P28004 |
PaxDbi | P28004 |
PRIDEi | P28004 |
PTM databases
iPTMneti | P28004 |
Interactioni
Subunit structurei
Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
Interacts with CLF1, PRP22 and PRP46.
Interacts with SPP382.
3 PublicationsBinary interactionsi
Hide detailsP28004
With | #Exp. | IntAct |
---|---|---|
PRP46 [Q12417] | 4 | EBI-20640,EBI-710 |
SYF1 [Q04048] | 3 | EBI-20640,EBI-540 |
YLR345W [Q06137] | 3 | EBI-20640,EBI-33827 |
Protein-protein interaction databases
BioGRIDi | 31734, 255 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex |
DIPi | DIP-2774N |
IntActi | P28004, 37 interactors |
MINTi | P28004 |
STRINGi | 4932.YAL032C |
Miscellaneous databases
RNActi | P28004, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P28004 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the SNW family.Curated
Phylogenomic databases
eggNOGi | KOG2441, Eukaryota |
GeneTreei | ENSGT00390000010423 |
HOGENOMi | CLU_006601_3_1_1 |
InParanoidi | P28004 |
OMAi | PDLQYDS |
Family and domain databases
InterProi | View protein in InterPro IPR017862, SKI-int_prot_SKIP IPR004015, SKI-int_prot_SKIP_SNW-dom |
PANTHERi | PTHR12096, PTHR12096, 1 hit |
Pfami | View protein in Pfam PF02731, SKIP_SNW, 1 hit |
i Sequence
Sequence statusi: Complete.
P28004-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFSNRLPPPK HSQGRVSTAL SSDRVEPAIL TDQIAKNVKL DDFIPKRQSN
60 70 80 90 100
FELSVPLPTK AEIQECTART KSYIQRLVNA KLANSNNRAS SRYVTETHQA
110 120 130 140 150
PANLLLNNSH HIEVVSKQMD PLLPRFVGKK ARKVVAPTEN DEVVPVLHMD
160 170 180 190 200
GSNDRGEADP NEWKIPAAVS NWKNPNGYTV ALERRVGKAL DNENNTINDG
210 220 230 240 250
FMKLSEALEN ADKKARQEIR SKMELKRLAM EQEMLAKESK LKELSQRARY
260 270 280 290 300
HNGTPQTGAI VKPKKQTSTV ARLKELAYSQ GRDVSEKIIL GAAKRSEQPD
310 320 330 340 350
LQYDSRFFTR GANASAKRHE DQVYDNPLFV QQDIESIYKT NYEKLDEAVN
360 370
VKSEGASGSH GPIQFTKAES DDKSDNYGA
Sequence cautioni
The sequence CAA44455 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62577 Genomic DNA Translation: CAA44455.1 Frameshift. U12980 Genomic DNA Translation: AAC05000.1 BK006935 Genomic DNA Translation: DAA06956.1 |
PIRi | S23409 |
RefSeqi | NP_009370.1, NM_001178177.1 |
Genome annotation databases
EnsemblFungii | YAL032C_mRNA; YAL032C; YAL032C |
GeneIDi | 851201 |
KEGGi | sce:YAL032C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62577 Genomic DNA Translation: CAA44455.1 Frameshift. U12980 Genomic DNA Translation: AAC05000.1 BK006935 Genomic DNA Translation: DAA06956.1 |
PIRi | S23409 |
RefSeqi | NP_009370.1, NM_001178177.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5GM6 | electron microscopy | 3.50 | P | 1-379 | [»] | |
5GMK | electron microscopy | 3.40 | P | 1-379 | [»] | |
5LJ3 | electron microscopy | 3.80 | K | 1-379 | [»] | |
5LJ5 | electron microscopy | 3.80 | K | 1-379 | [»] | |
5LQW | electron microscopy | 5.80 | M | 1-379 | [»] | |
5MPS | electron microscopy | 3.85 | K | 1-379 | [»] | |
5MQ0 | electron microscopy | 4.17 | K | 1-379 | [»] | |
5WSG | electron microscopy | 4.00 | P | 1-379 | [»] | |
5Y88 | electron microscopy | 3.70 | Q | 1-379 | [»] | |
5YLZ | electron microscopy | 3.60 | Q | 1-379 | [»] | |
6BK8 | electron microscopy | 3.30 | E | 1-379 | [»] | |
6EXN | electron microscopy | 3.70 | K | 1-379 | [»] | |
6J6G | electron microscopy | 3.20 | P | 1-379 | [»] | |
6J6H | electron microscopy | 3.60 | P | 1-379 | [»] | |
6J6N | electron microscopy | 3.86 | P | 1-379 | [»] | |
6J6Q | electron microscopy | 3.70 | P | 1-379 | [»] | |
SMRi | P28004 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31734, 255 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex |
DIPi | DIP-2774N |
IntActi | P28004, 37 interactors |
MINTi | P28004 |
STRINGi | 4932.YAL032C |
PTM databases
iPTMneti | P28004 |
Proteomic databases
MaxQBi | P28004 |
PaxDbi | P28004 |
PRIDEi | P28004 |
Genome annotation databases
EnsemblFungii | YAL032C_mRNA; YAL032C; YAL032C |
GeneIDi | 851201 |
KEGGi | sce:YAL032C |
Organism-specific databases
EuPathDBi | FungiDB:YAL032C |
SGDi | S000000030, PRP45 |
Phylogenomic databases
eggNOGi | KOG2441, Eukaryota |
GeneTreei | ENSGT00390000010423 |
HOGENOMi | CLU_006601_3_1_1 |
InParanoidi | P28004 |
OMAi | PDLQYDS |
Miscellaneous databases
PROi | PR:P28004 |
RNActi | P28004, protein |
Family and domain databases
InterProi | View protein in InterPro IPR017862, SKI-int_prot_SKIP IPR004015, SKI-int_prot_SKIP_SNW-dom |
PANTHERi | PTHR12096, PTHR12096, 1 hit |
Pfami | View protein in Pfam PF02731, SKIP_SNW, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP45_YEAST | |
Accessioni | P28004Primary (citable) accession number: P28004 Secondary accession number(s): D6VPI6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome I
Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names