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UniProtKB - P28002 (COMT1_MEDSA)
Protein
Caffeic acid 3-O-methyltransferase
Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Functioni
Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid (Probable) (PubMed:1898010).
The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins (Probable) (PubMed:1898010).
1 Publication1 PublicationCatalytic activityi
- EC:2.1.1.681 PublicationThis reaction proceeds in the forward1 Publication direction.
- (E)-5-hydroxyferulate + S-adenosyl-L-methionine = E-sinapate + H+ + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
: phenylpropanoid biosynthesis Pathwayi
This protein is involved in the pathway phenylpropanoid biosynthesis, which is part of Aromatic compound metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway phenylpropanoid biosynthesis and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 131 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 208 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 231 | S-adenosyl-L-methioninePROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 251 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 252 | S-adenosyl-L-methionine; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 265 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Active sitei | 269 | Proton acceptorPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Active sitei | 297 | By similarity | 1 | |
Active sitei | 329 | By similarity | 1 |
GO - Molecular functioni
- caffeate O-methyltransferase activity Source: UniProtKB-EC
- protein dimerization activity Source: InterPro
- S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB
GO - Biological processi
- lignin biosynthetic process Source: UniProtKB-KW
- methylation Source: UniProtKB-KW
- phenylpropanoid biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Lignin biosynthesis |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.68, 3078 |
UniPathwayi | UPA00711 |
Names & Taxonomyi
Protein namesi | Recommended name: Caffeic acid 3-O-methyltransferase1 Publication (EC:2.1.1.681 Publication)Short name: CAOMTCurated Short name: COMT1 Publication Alternative name(s): S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferaseCurated |
Organismi | Medicago sativa (Alfalfa) |
Taxonomic identifieri | 3879 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fabales › Fabaceae › Papilionoideae › 50 kb inversion clade › NPAAA clade › Hologalegina › IRL clade › Trifolieae › Medicago |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063204 | 1 – 365 | Caffeic acid 3-O-methyltransferaseAdd BLAST | 365 |
Proteomic databases
PRIDEi | P28002 |
Expressioni
Tissue specificityi
More abundant in roots and stems.1 Publication
Inductioni
By infection, plant wounding, or elicitor treatment of cell cultures.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein dimerization activity Source: InterPro
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P28002 |
SMRi | P28002 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P28002 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 130 – 136 | Substrate bindingCurated | 7 | |
Regioni | 162 – 180 | Substrate bindingCuratedAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.Curated
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR012967, Plant_MeTrfase_dimerisation IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF08100, Dimerisation, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P28002-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSTGETQIT PTHISDEEAN LFAMQLASAS VLPMILKSAL ELDLLEIIAK
60 70 80 90 100
AGPGAQISPI EIASQLPTTN PDAPVMLDRM LRLLACYIIL TCSVRTQQDG
110 120 130 140 150
KVQRLYGLAT VAKYLVKNED GVSISALNLM NQDKVLMESW YHLKDAVLDG
160 170 180 190 200
GIPFNKAYGM TAFEYHGTDP RFNKVFNKGM SDHSTITMKK ILETYTGFEG
210 220 230 240 250
LKSLVDVGGG TGAVINTIVS KYPTIKGINF DLPHVIEDAP SYPGVEHVGG
260 270 280 290 300
DMFVSIPKAD AVFMKWICHD WSDEHCLKFL KNCYEALPDN GKVIVAECIL
310 320 330 340 350
PVAPDSSLAT KGVVHIDVIM LAHNPGGKER TQKEFEDLAK GAGFQGFKVH
360
CNAFNTYIME FLKKV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | I → N in ACY06328 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63853 mRNA Translation: AAB46623.1 GU066087 mRNA Translation: ACY06328.1 |
PIRi | T09673 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63853 mRNA Translation: AAB46623.1 GU066087 mRNA Translation: ACY06328.1 |
PIRi | T09673 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KYW | X-ray | 2.40 | A/C/F | 1-365 | [»] | |
1KYZ | X-ray | 2.20 | A/C/E | 1-365 | [»] | |
AlphaFoldDBi | P28002 | |||||
SMRi | P28002 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PRIDEi | P28002 |
Enzyme and pathway databases
UniPathwayi | UPA00711 |
BRENDAi | 2.1.1.68, 3078 |
Miscellaneous databases
EvolutionaryTracei | P28002 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR012967, Plant_MeTrfase_dimerisation IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF08100, Dimerisation, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | COMT1_MEDSA | |
Accessioni | P28002Primary (citable) accession number: P28002 Secondary accession number(s): D0VDZ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 100 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families